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Summary of Contents for celvivo CLINOSTAR 30003
- Page 1 CLINOSTAR USER & SERVICE MANUAL MODEL 30003...
- Page 2 CelVivo makes no representations or warranties with respect to this manual. In no event shall CelVivo be held liable for any damage, direct or incidental, arising out of, or related to, the use of this manual.
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Page 3: Table Of Contents
TABLE OF CONTENTS PREFACE ..............................................6 GENERAL NOTES ........................................6 Identification of the device and its certification..........................6 CE Certification ........................................6 WEEE ............................................. 6 UL Approval ..........................................6 Electrical data - Power supply .................................... 7 Electrical data - ClinoStar ...................................... 7 ClinoStar Specifications ...................................... - Page 4 Using a bridge-device ..................................38 3.5. Connecting your ClinoStar to the CelVivo.Cloud ......................38 3.5.1. Creating a CelVivo.Cloud user and your CelVivo.Cloud Lab ..................39 3.5.2. Adding an additional ClinoStar to the CelVivo.Cloud Lab ................... 41 3.5.3. Inviting more users to your Lab ..............................41 3.5.4.
- Page 5 4.3. Operating the ClinoStar ..................................46 4.3.1. The "Identity" Controls ..................................47 4.3.2. The "Overview" Controls ..................................47 4.3.2.1. Initiating a video-stream from a ClinoReactor ........................48 4.3.3. The "Axles" Controls ....................................49 4.3.4. The “Climate" controls ..................................50 4.3.5. Thresholds and logic for alarms ..............................
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Page 6: Preface
This product has been certified by UL in compliance with relevant standards as described in the Declaration of Conformity. This product is or may be covered by one or more pending patent applications (see www.celvivo.com/patents) for one or more countries including the US. -
Page 7: Electrical Data - Power Supply
Electrical data - Power supply Electrica l data Ra ted Volta ge [V] 100 - 240 V Power frequency [Hz] 50-60 Hz Nomina l power [A] 1.8A Power cord length 2 meters Appliance Cla ss Class I equipment Pollution Degree Overvolta ge ca tegory Electrical data - ClinoStar Electrica l data... - Page 8 CO₂-da ta CO₂ range [Vol.-% CO₂] Ambient to 10 % CO₂ mea surement CO₂ ca libra tion Factory calibrated for 10 years O₂/N₂-da ta (If Hypoxia-module present) Oxygen ra nge [Vol.% O₂ a djusted by 2-21% (Ambient) N₂) O₂ mea surement Luminescence–based optical technology O₂...
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Page 10: Applicability Of The Instructions
WARRANTY Requirements for warranty CelVivo warrants the operational safety and functions of the ClinoStar only under the condition that: • the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, the device is not modified, •... -
Page 11: Explanation Of Safety Information And Symbols
EXPLANATION OF SAFETY INFORMATION AND SYMBOLS Safety information and symbols used in the operating instructions WARNING Indicates a hazardous situation which, if not avoided, could result in death or serious injuries. CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries. -
Page 12: Symbols On The Device
Symbols on the device European Union CE mark Waste Electrical and Electronic Equipment Directive 2012/19/EU Equipment contains a UV-C light source. Turn off the UV lamp before servicing. Refer to operating instructions to avoid hazardous situations. The operating instructions should be considered when operating the device. UL approval mark... -
Page 13: Intended Purpose Of The Device
Do not use this equipment in a hazardous atmosphere or hazardous with materials for which the equipment was not designed. CelVivo is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by CelVivo. -
Page 14: Incorrect Use
Release hazardous microorganisms or viruses that might be able to transverse a 0.22 µm pore size filter. Do not use consumables or parts longer than recommend by CelVivo. This may jeopardize the safety of the persons operating the system. Using consumables longer than recommended might also jeopardise the quality or validity of the results obtained. -
Page 15: Safety Notes On Gases
Applicable safety data sheets, hygiene guidelines, and technical regulations (National, local, or • institutional) which are in force in the user’s location. • The user must also instruct any cleaning personnel how to clean this instrument and issue personal safety instructions to any other persons who may come in contact with the instrument. -
Page 16: Safety Notes On Carbon Dioxide (Co₂) And Nitrogen (N )
Safety notes on carbon dioxide (CO₂) and nitrogen (N CO₂ is rated as a harmful gas. Therefore, certain safety instructions must be observed when the ClinoStar is installed and operated. Safety notes on nitrogen (N This instrument can be adapted to use N to displace O₂... -
Page 17: Introduction
INTRODUCTION Thank you for selecting CelVivo products for your 3D cell culture needs. The CelVivo ClinoStar incubator is designed for use in industry, university, hospital and school laboratories or wherever there is a need to reliably culture cells in a clinostat in an accurately and reproducibly temperature and/or CO₂ and/or O... -
Page 18: Product Description
1.1. Product description The product consists of a clinostat-incubator unit, the ‘ClinoStar’ and is operated by a client through a browser. The ClinoStar is an incubator specifically designed for long-term culture of multicellular structures (e.g. as biopsies, organoids or spheroids). It uses the clinostat principle to keep them in shear-stress free suspension. -
Page 19: Clinostar Overview
1.3. ClinoStar Overview Figure 1 Principle components of the ClinoStar Glass walled door UV-C light source Camera window Door hatch Door gasket Stainless steel incubation chamber Front LED light window USB port (2) (Insert) Hypoxia module Refer to separate document on how to install hypoxia. (Insert) O sensor... - Page 20 Figure 2 ClinoReactor and holder Lighthouse UV-C light source Temperature sensor CO₂ sensor Back LED light (in axle behind the ClinoReactor holder)
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Page 21: Sensor System
Figure 3 CO₂ connection RJ45 internet cable connection USB socket Air vent Power supply socket Slots for stacking bars (used when you stack more than one ClinoStar) Reboot button Power supply bay (use Velcro straps to attach power supply to device) Cooling fan 1.4. -
Page 22: Fan System
Blue Continuous Normal Blue 1 sec. Normal – ClinoStar is being used on CelVivo.Cloud or through the Local Control Panel. Bright Blue 1 sec for 10 sec. The “Identify” function has been activated on the Local Control Panel or through the CelVivo.Cloud. -
Page 23: Camera System
All you need to access the CelVivo.Cloud web application is an internet connected device and a browser. Refer to sections 3.3, 3.4 and 3.6 to set this up. -
Page 24: Unpacking, Placing And Preparing The Equipment
2. UNPACKING, PLACING AND PREPARING THE EQUIPMENT CAUTION! RISK OF PERSONAL INJURY! The ClinoStar system including packaging weights approximately 35 kg and can be safely transported by a pallet lifter or similar. When unpacked, the ClinoStar weighs approximately 25 kg. and should be moved by at least two persons. -
Page 25: Arrival And Unpacking
2.1. Arrival and unpacking Upon arrival check the integrity of the shipping box. In case of any damage, take some pictures and report it to CelVivo and to the shipping company immediately (without unpacking it). If packing is undamaged and the shock-watch does not indicate impacts during transportation, unpack it, and check the unit and any optional accessories (see delivery receipt for list), for completeness and for absence of damage. - Page 26 Power supply and electrical cord Manufacturer: FSP Technology, model: FSP120-AAAN3 CO₂ and N₂ hose Ø8 mm (o.d) 2 meter CO₂ filter WiFi antenna User & service manual (this manual)
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Page 27: Content Of Hypoxia Package (Optional)
2.1.2. Content of Hypoxia package (optional) If you have ordered the Hypoxia unit together with your ClinoStar the unit will be preinstalled. The auxiliary parts will be delivered in a separate box. If you have ordered the Hypoxia module as an add-on module, you will receive the unit in a box including all required auxiliary parts and a quick install guide. -
Page 28: Installation Requirements
2.2. Installation requirements The ClinoStar is designed for indoor use only. Requirements for the installation site for the ClinoStar are given below. WARNING! Installing the ClinoStar in an environment that does not adhere to the requirements set forth in this section might damage the unit. The ambient temperature of the installation site must be 19 °C or above to avoid condensation occurring inside the ClinoStar which can result in failure of electronical and/or mechanical components. - Page 29 The relative humidity should not exceed 70% (max.) and the conditions must not be • condensing. • The unit must not be exposed to direct sunlight. The minimal distance to adjacent surfaces which must be observed are given in figure 6 •...
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Page 30: Special Requirements For Stacking
ClinoStars can be stacked on top of each other, up to a limit of three units. The bearing surface must be able to carry the combined weight of the ClinoStars and accessories (approximately 25 kg per unit). For safe stacking, use the original CelVivo wall-mounts. Contact your representative for more information if required. -
Page 31: Power Connection
2.7. Network connection The ClinoStar can be connected to the CelVivo.Cloud through ethernet cable, through WiFi or through a relay-device like a tablet or computer in cases of more advanced security protocols. Refer to later sections regarding specific setup instructions for the various connection types. -
Page 32: Installation
3. INSTALLATION If you have purchased the hypoxia module, it is a good idea to mount this module before you install the ClinoStar. If you are mounting the hypoxia module onto an existing ClinoStar, unplug the ClinoStar from the electrical supply and pull it out away from the wall or unhook from the wall mount. 3.1. -
Page 33: Installing And Connecting The Clinostar
3.2. installing and connecting the ClinoStar WARNING! RISK OF PERSONAL INJURY Use a CO₂/O alarm system for the lab if the room is not properly ventilated. Wear personal protective equipment (PPE). Check the tube connection system with a leakage test. During normal use, elevated levels of CO₂ and or reduced O₂ levels may be found in and around the operating area of the ClinoStar WARNING! INCORRECT GAS INSTALLATION OR INSUFFICIENT... - Page 34 4. Connect the hose to the two-stage CO₂ pressure regulator (which is in turn connected to the outlet valve of the gas supply outlet). Connecting N (if equipped with Hypoxia) 1. Take the brass pressure regulator out of the shipping box. 2.
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Page 35: Creating A Local Connection To The Clinostar
Tablet) and the specific ClinoStar. This can be used to control the ClinoStar through a dedicated device in the Lab or if no internet connection is available. Even though you plan to use the CelVivo.Cloud to control your ClinoStar, establishing a local connection might be required to setup your internet connection. -
Page 36: Changing The Local Connection Settings
To get the maximum value of your new ClinoStar we strongly recommend that you connect the ClinoStar to the internet. This allows you to utilize the full value of CelVivo.Cloud including advanced logging, remote control, e-mail alarm-notifications, software updates, etc. -
Page 37: Setting Up An Ethernet Connection
3.4.1. Setting up an ethernet connection If you wish to connect your ClinoStar using an ethernet connection, simply plug an ethernet cable into the ethernet port on the back of the ClinoStar (point B in figure 3). Consult your IT-department prior to connecting any devices. -
Page 38: Using A Bridge-Device
Before setting up bridging please consult your IT-department to ensure that you are in line with your institution’s IT-policy. 3.5. Connecting your ClinoStar to the CelVivo.Cloud When the ClinoStar is connected to CO2, (N2), and a power supply, it is ready to go. When turned on, the... -
Page 39: Creating A Celvivo.cloud User And Your Celvivo.cloud Lab
PC or tablet. Refer to section 3.4 on setting up this connection. 3.5.1. Creating a CelVivo.Cloud user and your CelVivo.Cloud Lab Before you can add the ClinoStar to the CelVivo.Cloud you need to create a username and password as well as setting up a “virtual lab”. This step only needs to be done once. - Page 40 ClinoStar to the lab. Click Submit and wait. Figure 18 The CelVivo.Cloud will now add your ClinoStar to the newly created Lab and log you into the CelVivo.Cloud online experience. The process can take 2-3 minutes to complete.
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Page 41: Adding An Additional Clinostar To The Celvivo.cloud Lab
3.5.3. Inviting more users to your Lab If you need to invite more people to your Lab, you can easily do that through the CelVivo.Cloud account. When inviting a new user, you must decide if they should get the role as Lab Manager or a Lab User. -
Page 42: After Installation
Plea se note: If you are inviting a CelVivo.Cloud user, that is already registered in another Lab, they will receive a notification next time they sign-on to CelVivo.Cloud where they can accept or reject the invitation to your Lab. 3.5.4. After installation After the ClinoStar is properly positioned and connections have been made to control units, clean and disinfect the incubator interior and exterior (see section 5. -
Page 43: Operation
ClinoReactor can be viewed at the same time. Please also note, that the camera can only serve one channel at the time (Local Controls or CelVivo.Cloud). If you wish to have multiple persons looking at the same feed at the time same (ie for discussing results by video- or teleconference) this can only be done through the CelVivo.Cloud. -
Page 44: Navigating The Celvivo.cloud
(4) of the CelVivo.Cloud. 4.2.2. The ClinoStars overview screen When navigating to the ClinoStars section of the CelVivo.Cloud you will get a list of ClinoStars assigned to your lab. On the overview screen you can Assign a... -
Page 45: A Note On Working Online
Clinostar. Refer to section 4.3 for an introduction on how to control the ClinoStar. shows if the ClinoStar is “Online” on the CelVivo.Cloud platform. If its green the ClinoStar is connected. If the icon is red the ClinoStar is not connected and hence can’t be remotely controlled through CelVivo.Cloud. -
Page 46: Operating The Clinostar
If you are accessing the ClinoStar through the Local Control panel you are directed directly to the ClinoStar Control Panel when connecting. If you are connecting through the CelVivo.Cloud click the “Control ClinoStar” (marked “6” in figure 25 in section 4.2.2) -
Page 47: The "Identity" Controls
ClinoStar. In the top-right corner you can see whether the ClinoStar is connected to the CelVivo.Cloud. A green connection symbol shows that the ClinoStar is connected. A red connection symbol that connection has been lost. -
Page 48: Initiating A Video-Stream From A Clinoreactor
ClinoStar at the same time. Likewise, it is not possible to stream through the CelVivo.Cloud while also using the stream through the Local Control Panel. The camera feed will automatically turn-off, including the associated light in the ClinoStar when the user session expires. -
Page 49: The "Axles" Controls
ClinoStar and the device used to control the ClinoStar. A few seconds of delay must be expected when using the CelVivo.Cloud video stream. Hence, when adjust speed or rotation using the CelVivo.Cloud please allow a few seconds for the stream to reflect the changes made. 4.3.3. The "Axles" Controls This screen allows you to control the speed and direction of the 6 ClinoReactors individually. -
Page 50: The "Climate" Controls
Climate Setting will turn Red. In the example to the left, the CO₂ level has fallen to below the lower limit. Additionally, a warning message will appear at the top of the navigation window. If you are using the CelVivo.Cloud the alarm- information will also be shown on the frontpage. Figure 38 Figure 39... -
Page 51: The "Decontamination" Controls
To alert those in the lab, the LED on the appropriate ClinoStar door will flash red. Finally, if the ClinoStar is connected to the CelVivo.Cloud, anyone with Lab Manager status for that particular Lab will receive an email notification that an alarm has triggered (unless he/she has disabled this feature). -
Page 52: The "Configuration Controls
Figure 41 4.3.7. The "Configuration Controls" If you have the role of Lab Manager, the Configuration Controls are available through CelVivo.Cloud. All users have access through the Local Control Panel. Through these controls you can update the Operating System and Software of the ClinoStar (Updates can only be triggered through CelVivo.Cloud connection as they require internet access), run a factory-reset or reboot the ClinoStar. -
Page 53: The Factory Reset Menu
Lab in CelVivo.Cloud Figure 45 Once a ClinoStar is in RESET-state it can be unassigned from the CelVivo.Cloud by a Lab Manager (refer to figure 45) and available for assignment into another Lab. You will be able to control the ClinoStar through the Local Control Panel even though it is in a RESET state... -
Page 54: The Reboot Menu
If you need to perform a reset but do not have access to the CelVivo.Cloud or Local Control Panel refer to section 4.4 on how to execute a hardware triggered soft reset. 4.3.7.3. The Reboot Menu Using the reboot menu, you can request a reboot of the ClinoStar. -
Page 55: Performing A Hardware-Reset
CelVivo.Cloud. Refer to section 4.3.6 on the Configuration Controls for instructions on how to do this. If you do not have access to the Local Control Panel or you cannot connect to the CelVivo.Cloud and access the machine, you can contact CelVivo Support and receive a reset token. The reset-tokens can be placed... - Page 56 ClinoStar on CelVivo.Cloud. If required, the unassignment needs to be done by a Lab Manager following a reset procedure. Once a ClinoStar is in RESET-state it can be unassigned from the CelVivo.Cloud and will be available for assignment into another Lab.
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Page 57: Cleaning And Disinfection
5. CLEANING AND DISINFECTION Regular cleaning of the ClinoStar, while sometimes a chore, is a necessity to help protect your cells from contamination and to keep the incubator functioning properly. Carefully following these simple steps will keep your incubator clean, help reduce chances of contamination, and help keep your cells growing well. -
Page 58: Tablet
4. Clean all the internal surfaces, axles, inner door, fan and door gaskets with mild, warm and soapy water. Be sure to reach all the corners and crevices where dirt, dust and germs can hide. An incubator with curved ‘corners’ makes this task easier. Remember to clean the back of the black ClinoReactor holders and the inner surface of the door and its gasket! Rinse all surfaces and parts using a cloth, wet with distilled water and gently wrung. -
Page 59: User Maintenance And Service
CelVivo. The unit is designed to need a minimum of maintenance by the user. All other service should be carried out by a CelVivo approved technician. The user should regularly perform the following checks: 6.1. Cleaning Perform according to use –... -
Page 60: Door Sealing Gasket
When using gas with a technical grade of 99.5 %, we recommend changing the gas filter once a year. When using gases with a lower purity, the filter should be changed more frequently. Please consult CelVivo. 6.4. Door sealing gasket Perform yearly. -
Page 61: Spare Parts And Accessories
7. SPARE PARTS AND ACCESSORIES 7.1. Spare parts and accessories for the ClinoStar Description Number Product number 41323-286 Door Gasket 41323-221 Gas filter (on hose) 41900-001 Hose 41900-002 RJ45 network cable (3 meters) 41900-003 Wireless Dongle 41902-004 Control Unit (tablet) 31001 Hypoxia module 30021... -
Page 62: Disposal
8. DISPOSAL 8.1. Disposal of the transport packing Packing element Material How to Dispose Door protection PE foam Plastic recycling Packing box Wood Wood recycling Insulating air cushion foil PE foil Plastic recycling PE foil Plastic recycling If recycling is not possible, all packing parts can also be disposed of with normal waste. CAUTION CONTAMINATION HAZARD! As the device can have been used for processing and treating infectious substances... -
Page 63: Disposal Of A Clinostar
8.2. Disposal of a ClinoStar This product complies with the European Union Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC and should be disposed of accordingly. The ClinoStar contain reusable materials and should be disposed of accordingly. All components, with the exception of the HEPA filters, can be disposed of after having been thoroughly cleaned and disinfected. -
Page 64: How To Get Help
There are several ways to get help. Please choose the most appropriate way for your needs. For Genera l Guida nce: User login at CelVivo.com. Have a look at this restricted region of the website where we will post a growing number of useful SOPs, tips and hints from other users. -
Page 65: Frequently Asked Questions
10. FREQUENTLY ASKED QUESTIONS As a rule of thumb, when you start to grow cells in 3D in the ClinoReactor we would recommend that you continue to use the same conditions for the cells as they are used to (media, additives, temperature, CO₂% and O₂%). - Page 66 Q: Have you ever experienced that spheroids/organoids quickly adhere to the ClinoReactor surface e.g. during gardening or image acquisition? Would you say this is cell type specific? In general, the materials used for our ClinoReactor are very low attachment materials. If cells/spheroids/ organoids are attaching to the ClinoReactor plastic surface, we would speculate that some kind of protein coating has been deposited on the plastic.
- Page 67 Please also refer to our FAQ at www.celvivo.com/support If you have further questions or tips, we would love to hear them. We will pass them on to all ClinoStar users and hopefully save them time and effort.
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