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SCIEX OptiMS Thermo MS Installation Manual

SCIEX OptiMS Thermo MS Installation Manual

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OptiMS Thermo MS Adapter
For Nanospray II, Nanospray Flex, and Nanospray Flex NG Ion
Sources
Installation Guide
RUO-IDV-05-3313-B | 5302405
December 2023

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Summary of Contents for SCIEX OptiMS Thermo MS

  • Page 1 OptiMS Thermo MS Adapter For Nanospray II, Nanospray Flex, and Nanospray Flex NG Ion Sources Installation Guide RUO-IDV-05-3313-B | 5302405 December 2023...
  • Page 2 SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are the sole and exclusive representations, warranties, and obligations of SCIEX. SCIEX...

  • Page 3: Table Of Contents

    6 Prepare the Buffer Trays......................42 7 Prepare the System........................ 43 Install the Cartridge.........................43 Remove and Install the Interface Plate................48 Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 3/83...
  • Page 4 9 Shut Down and Disconnect....................78 Remove the Cartridge......................78 Store the Cartridge........................82 Disconnect the CESI 8000 Plus System from the Mass Spectrometer........82 OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...

  • Page 5: Overview

    Applicable labels have been affixed to the system. International Standards The OptiMS Thermo MS Adapter for NanoSpray II, NanoSpray Flex, and NanoSpray Flex NG Ion Sources has been evaluated as part of the CESI 8000 Plus High Performance Separation-...

  • Page 6: Decommissioning And Disposal

    When the system is removed from service, obey national and local environmental regulations to divide and recycle different materials. Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE to get a copy of the form.

  • Page 7: Symbols, Indicators, And Labels

    Definition Hot Surface Hazard Symbols, Indicators, and Labels Note: If any of the labels used to identify a component become detached, then contact a SCIEX service representative. Documentation Symbols and Conventions The following symbols and conventions are used throughout the guide.
  • Page 8 Tip! Tips supply information that helps to apply the techniques in a procedure or gives a shortcut, but that is not essential to the completion of a procedure. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 9: Install The Adapter

    Use the manufacturer's instructions to install the ion source on the mass spectrometer. Pull the ion source drawer fully out, away from the inlet on the mass spectrometer. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 10 The top of the arm on the mounting bracket should be parallel with the horizontal plane of the XYZ manipulator. Use the alignment knobs on the XYZ manipulator to do an initial alignment: OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 11 Figure 2-3 Alignment of Rulers: Knob A Use knob B to align 14 on the moveable ruler with 0 on the static ruler. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 12 Install the adapter in the mounting bracket until the adapter is tight against the bracket. Make sure that the high-voltage cable hangs freely from the adapter. If required, then turn the adapter. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 13 Figure 2-8 Adapter Installed in the Mounting Bracket Push the ion source drawer toward the inlet on the mass spectrometer. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 14: Connect The Cables

    Connect the other end of the relay cable (Figure 2-10, item 1) to the mass spectrometer. Figure 2-10 Relay Cable OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...
  • Page 15 Connect the input cable between the ion source and the CESI 8000 Plus system. Connect the high-voltage input cable to the port on the ion source. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 16 Connect the red banana plug to the red port on the connections panel. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 17 Connect the black banana plug to the black port on the connections panel. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 18: Install The Adapter (Nanospray Ii)

    Make sure that the 1-inch adapter ring is not installed. Figure 2-13 Adapter Ring Item Description Socket head screws Ion max adapter 1-inch (deep) adapter ring OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405 18/83...
  • Page 19 Use the alignment knob to move the ion source stage as far away as possible from the inlet on the mass spectrometer. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 20 If the ion source stage is too close to the inlet on the mass spectrometer, then damage to the sprayer tip can occur. Install the adapter in the holder on the ion source. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 21 Make sure that the position of the adapter is tight, and then tighten the thumbscrew. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 22 Adapter installed in the ion source Remove the protective sleeve from the sprayer tip. Figure 2-18 Protective Sleeve and Sprayer Tip OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...
  • Page 23 10. Turn the sprayer assembly counterclockwise to lock it in the adapter. Figure 2-19 Lock the Sprayer Assembly in the Adapter Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 24: Connect The Cables

    On the right side of the CESI 8000 Plus system, open the access door to show the high- voltage connections panel. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...
  • Page 25 90 degrees clockwise to lock it in position. Connect the red banana plug to the red port on the connections panel. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 26 Connect the high-voltage input cable from the mass spectrometer to the Input port on the connections panel, and then turn the cable 90 degrees clockwise to lock it in position. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 27: Change The Mass Spectrometer Type

    Turn on power to the CESI 8000 Plus system. Click Start > CESI 8000 Software, and then click Change MS Type. Figure 3-1 Change MS Type Option Make sure that the Connect to AB SCIEX MS check box is cleared. Figure 3-2 Change MS Type Dialog Installation Guide...
  • Page 28 If the IP address that shows needs to be changed, then contact sciex.com/request-support. Click Change. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...

  • Page 29: Align The System With The Mass Spectrometer

    Make sure that the capillary will not touch the mass spectrometer when the stage moves forward. Figure 4-1 Nanospray II Alignment Knob Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 29/83...
  • Page 30 Make sure that the CESI 8000 Plus system is approximately 4 inches (10 cm) away from the ion source stage so that the coolant tubes can be extended fully. Figure 4-2 System Near the Ion Source Stage OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 31 CESI 8000 Plus system aligns with the sprayer tip. WARNING! Personal Injury Hazard. Remove consumables from the mobile cart before the height is adjusted. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 31/83...
  • Page 32 When the CESI 8000 Plus system is in visual alignment with the mass spectrometer, step on the locking lever of each caster to lock each wheel of the mobile cart in position. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide...
  • Page 33 If the sprayer tip is too close to the inlet, then the accidental suction of rinse solution into the mass spectrometer can occur and cause damage. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 33/83...

  • Page 34: Align The System With The Mass Spectrometer (Nanospray Flex And Nanospray Flex Ng)

    Make sure that the CESI 8000 Plus system is approximately 18 cm to 20 cm (7.1 inches to 7.9 inches) away from the ion source so that the coolant tubes can be extended fully. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide...
  • Page 35 When the CESI 8000 Plus system is in visual alignment with the mass spectrometer, step on the locking lever of each caster to lock each wheel of the mobile cart in position. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 36 While holding the body of the adapter to prevent it from moving towards the inlet, install the sprayer in the adapter, aligning the arrow on the cartridge with the unlock position on the adapter. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 37 Move the sprayer tip until it is approximately 2 mm to 4 mm away from the ion source inlet. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 37/83...
  • Page 38 Align the System with the Mass Spectrometer Figure 4-12 Sprayer Tip at the Ion Source Inlet OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405 38/83...

  • Page 39: Prepare The Reagents

    Only prepare new 20% HAc solution when the 200 mM LE buffer, pH 4.0 is prepared. In a 100 mL glass bottle, add 80 mL of CE Grade Water. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 40: Prepare The 200 Mm Le Buffer, Ph 4.0

    Divide the beta-galactosidase stock solution into aliquots of 50 µL. Keep the beta-galactosidase stock solution at –35 °C to –15 °C for as long as 1 year. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 41: Prepare The Beta-Galactosidase Test Sample

    Keep the sample at 2 °C to 8 °C. At the end of the day, discard the solution that was not used. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 42: Prepare The Buffer Trays

    Note: This layout is the same for all five methods in the CESI 8000 Plus software. Figure 6-1 Buffer Tray Layout OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 43: Prepare The System

    Open the cartridge cover. Figure 7-1 Cartridge Cover Loosen the thumbscrews on the insertion bar, and then lift the insertion bar. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 43/83...
  • Page 44 Open the access panel. Figure 7-3 Access Panel Put the sprayer housing through the side panel, and then put the sprayer in the holster. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 45 To install the cartridge body, put the ends of the cartridge on the aligning blocks of the interface plate at a 45-degree angle, and lightly push the cartridge in. Figure 7-5 Aligning Blocks Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 45/83...
  • Page 46 Figure 7-6 T Slider Item Description Align the T slider in the groove OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405 46/83...
  • Page 47 Capillaries can be seen below the interface block within the ejectors Put the coolant and capillary tubing through the notched arm. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 48: Remove And Install The Interface Plate

    Cartridge. Turn off the power to the CESI 8000 Plus system. Loosen the thumbscrews on the sides of the interface plate. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...
  • Page 49 Hold the handle on the top of the interface plate and install it. Make sure that the interface plate is flush with the mounting surface of the CESI 8000 Plus system. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 50: Condition The Capillaries

    Use the CESI-MS conditioning method to rehydrate the coating of the bare fused-silica capillaries and to establish the electrical connection in the sprayer tip. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 51 Carefully immerse the sprayer tip in the Falcon tube. Figure 7-13 Sprayer Immersed in Liquid Run the CESI-MS conditioning method. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 51/83...

  • Page 52: Establish A Stable Spray

    In the 32 Karat software, go to the Direct Control window. Click the Pressure field. Figure 7-15 Pressure Settings Dialog to Fill the Conductive Liquid Capillary OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 53 When the conductive liquid capillary is filled with BGE, a droplet will show at the end of the stainless steel needle. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 54 Figure 7-18 Pressure Settings Dialog to Fill the Separation Capillary In the Pressure field, type 100. In the Duration field, type 2. Click Forward. Click Pressure. Click OK. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405 54/83...

  • Page 55: Optimize The Esi Voltage

    To set the values for voltage in the 32 Karat software, do this: Go to the Direct Control window, and then double-click the Voltage field. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 56 In the Interface Heater Temperature (IHT) field, type 50.0. On the MS tab, do this: In the Scan type field, select TOF MS. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 57 In the TOF Masses (Da) Max field, type 2000. In the Period Duration field, type 10. Click Start. If a non-SCIEX mass spectrometer is in use, then do this on the mass spectrometer acquisition computer: Set the ESI voltage to zero.
  • Page 58 In the Period Duration field, type 5. 16. Click Acquire, and then type a file name (for example, Baseline). 17. If a non-SCIEX mass spectrometer is in use, then do this on the mass spectrometer acquisition computer: Set the ESI voltage to 1.0 kV and continue to acquire data.

  • Page 59: About Tune Files

    Fine-Tune the Position of the Sprayer Tip. 19. If a non-SCIEX mass spectrometer is in use, then do this: Monitor the spray stability for 20 minutes to 30 minutes to make sure that the baseline fluctuation is ≤ 40%. If the baseline fluctuation is > 40%, then condition the capillaries. Refer to the section: Condition the Capillaries.

  • Page 60: Fine-Tune The Position Of The Sprayer Tip

    Use this procedure to adjust the position of the sprayer tip and find the required ESI voltage for mass spectrometer acquisition: • After installing a cartridge OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 61 10. Set the ESI voltage to zero. 11. Turn off the separation voltage. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 61/83...

  • Page 62: Do A Test Separation

    As an alternative, PepCalMix can be used as the test sample. PepCalMix has 20 different stable isotope-labeled peptides and is available from SCIEX. For instructions about how to use PepCalMix, refer to the document: CESI 8000 Plus High Performance Separation-ESI Module User Guide.
  • Page 63 • The following figure shows the second segment of the tune method, with a duration of 44 minutes and an the optimum ESI voltage set for a stable spray. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 64 In the Scan Description section, from the Data type list, select Profile. In the Scan Ranges section, in the First Mass (m/z) field, type 300.00, and in the Last Mass (m/z) field, type 1500.00. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 65: Create And Start A Sequence With The Xcalibur Software

    Note: Make sure that the system will go into standby mode when the sequence completes. In the Xcalibur software, open the New Sequence Template window. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 66 Click OK. On the Sequence Setup-Home Page, highlight the row for the sample to run, and then click the Sequence Run icon OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...

  • Page 67: Manually Create The Cesi-Ms Separation Method

    In the 32 Karat software, click File > Method > New. Use the initial conditions in the following figure. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 67/83...
  • Page 68 In row 10 of the sequence table, click the Event cell, then select Separate. Set the parameters that show in the following figure, and then click OK. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 69: Create A Sequence In The 32 Karat Software

    Create a Sequence in the 32 Karat Software In the 32 Karat software, click File > Sequence > Sequence Wizard. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 70 To the right of the Method field, click , and then select the CESI-MS separation method. Click Next. Figure 8-10 Sequence Wizard - Unknowns Page OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405...
  • Page 71 Note: The Open File option is used to reprocess data files, not to acquire data. Select the applicable options. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 71/83...
  • Page 72 Note: If the Advance check box is selected for the inlet, outlet, or both vials, then the values for the vial positions are filled down automatically in the Sample Inject Inlet/Outlet columns in the sequence table. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 73: Load The Sample

    1.5 mL 0.1 N HCl 1 vial 1.5 mL Put the reagents in the following positions in the buffer tray. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 73/83...

  • Page 74: Start The Sequence In The 32 Karat Software

    To see the status of the first autocalibration run, monitor the Bgal_Installation sequence. The first autocalibration run in the following figure is being acquired. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 75: Analyze The Data

    Click File > Open, and then browse to the location where the beta-galactosidase data file was saved. Click Open. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 75/83...
  • Page 76 Note: If required, then use the mass spectrometer mass calibration to adjust the peak ranges 714.5 to 714.7 and 729.2 to 729.4. • Set automatic processing to include smoothing with a smoothing value of 5. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 77 Calculate the migration time of each peak. Make sure that the two peaks are separated by at least 0.05 minutes. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources...

  • Page 78: Shut Down And Disconnect

    Approximately 30 seconds are required. Wait for the pump to turn off. Loosen the thumbscrews on the insertion bar. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 79 Figure 9-3 Unlock the Sprayer from the Cartridge Item Description Sprayer in the Unlock position Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 79/83...
  • Page 80 11. Put the tubing with the sprayer end through the access panel. Figure 9-5 Sprayer and Tubing Through the Access Panel 12. Remove the coolant tubing from the notched arm. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources...
  • Page 81 Note: Drops of liquid coolant that might fall from the cartridge ends are normal and do not cause damage to the hardware. 14. Refer to the section: Store the Cartridge. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 81/83...

  • Page 82: Store The Cartridge

    Make sure that the cartridge has been removed. Refer to the section: Remove the Cartridge. Turn off the CESI 8000 Plus system. OptiMS Thermo MS Adapter For Nanospray II, Installation Guide Nanospray Flex, and Nanospray Flex NG Ion Sources RUO-IDV-05-3313-B | 5302405 82/83...
  • Page 83 11. Remove the adapter from the ion source. If required, then remove the adapter mounting bracket. 12. Keep the adapter, adapter mounting bracket and thumbscrews, and cables in a safe place. Installation Guide OptiMS Thermo MS Adapter For Nanospray II, RUO-IDV-05-3313-B | 5302405 Nanospray Flex, and Nanospray Flex NG Ion Sources 83/83...

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