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Summary of Contents for Azure Ao Absorbance
- Page 1 Azure Ao Absorbance Microplate Reader User Manual Rev 20201009...
- Page 2 Foreword Thank you for purchasing the Azure Ao Microplate Reader. This user manual describes how the instrument works and how it should be operated. Please read carefully before operating. Keep this user manual for reference in case you encounter problems.
- Page 3 Avoid obstructing the vent on the instrument as it is designed to provide aeration. When finished with the instrument, shut the unit off. Unplug when not in regular use and cover to prevent dust accumulation. Azure Ao Absorbance Microplate Reader User Manual Page 3...
- Page 4 Instrument is dropped or outer shell damaged • The function has obviously changed The Maintenance of Instrument Microplate holder and instrument outer shell can be cleaned using a cloth dampened with alcohol. Azure Ao Absorbance Microplate Reader User Manual Page 4...
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Page 5: Table Of Contents
Settings 6.4.1 Language Setting 6.4.2 Filter Setting 6.4.3 Maintenance 6.4.4 Time and Date setting Maintenance, Storage & Transportation Maintenance 7.1.1 Light Source Replacement 7.1.2 Add Optical Filter Storage and transportation Troubleshooting Azure Ao Absorbance Microplate Reader User Manual Page 5... -
Page 6: Brief Introduction
Brief Introduction The Azure Ao Absorbance Microplate Reader is a professional instrument for performing EIA tests, measuring concentrations, measuring absorbance, and performing Enzyme - Linked Immunosorbent Assays (ELISA). This type of reader is widely used in biology, agriculture, food and environment research, and is greatly beneficial to the growing number of ELISA kit users. -
Page 7: Features
CV≤0.3%(0 – 3Abs); CV≤2%(3 – 4Abs) Accuracy (450nm) ±0.005 or ±1%(0 – 3Abs), ±2%(3 – 4Abs) Channel Deviation <0.01A—450nm 1.0Abs Speed Single wavelength <15s/96well, double wavelength<28s/96well (Normal mode) Dimension (WXDXH) 440x295x225mm Weight (kg) 10kg Azure Ao Absorbance Microplate Reader User Manual Page 7... - Page 8 USB port: Use USB ports to export data from the instrument or to connect a printer, keyboard or mouse. • Back door: Access the light source or optical filter wheel. • Knob: Use the knob to open the back door of the instrument. Azure Ao Absorbance Microplate Reader User Manual Page 8...
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Page 9: Installation
Doing so may void warranty. Connect instrument to power supply. Instrument will start self-testing after powering on and will be immediately ready for use. Note: Do not connect instrument to an ungrounded outlet. Azure Ao Absorbance Microplate Reader User Manual Page 9... -
Page 10: Operation Guide
After completion of the self-check, instrument will enter the main software interface. See Fig 1. 4.2—Ao Software Overview The Azure Ao operates with an intuitive, touch-based user interface. All assay parameters and instrument functions can be accessed through four tabs at the top of the main interface – Protocol, Results, Settings, and Help. -
Page 11: The Results Tab
Export – Click to export data to USB. Print – Click to print using an Ao Printer. Azure Ao Absorbance Microplate Reader User Manual Page 11... -
Page 12: The Settings Tab
• Raw Intensity – Set instrument to return Optical Density or Raw light intensity measurements. Note: Other settings in this tab are password protected. Azure Biosystems does not recommend accessing these settings as they are reserved for use during the instrument manufacture process. -
Page 13: Operation Quick Guide
The protocol and data will be saved as a single file. After the plate is read, the software will automatically move to the Results tab. View, print or export raw data or analyzed data from this tab. Azure Ao Absorbance Microplate Reader User Manual Page 13... -
Page 14: Detailed Software Operation
Touch “File List” to retrieve saved program files. If the file was saved after reading a plate, selecting the saved file will open the program along with the saved plate data and results reading a plate. Figure 4 Azure Ao Absorbance Microplate Reader User Manual Page 14... - Page 15 Touch the selection box to the right of “Filter 1” to select a filter. See Fig 6. There are 5 standard filters included: 405nm, 450nm, 492nm, 595nm, and 630nm – as well as 3 additional customer selected filters. Choose one as Filter1. Figure 6 Azure Ao Absorbance Microplate Reader User Manual Page 15...
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Page 16: Layout
6.1.2—Layout Plate Layout 1. In the “Protocol” interface, you can touch the box to the right of “Plate Layout” to bring up the Plate Layout Interface. See Fig 8. Figure 8 Azure Ao Absorbance Microplate Reader User Manual Page 16... - Page 17 QC control. Steps for setting up the QC control samples are the same as above for setting standards. (See Fig 10). Figure 10 You can set up to 9 QC controls and can set several wells with the same number. Azure Ao Absorbance Microplate Reader User Manual Page 17...
- Page 18 Any mistake will affect curve fitting and the measured result. 3. “Unit” can be set by pressing ”Unit” button on the right. See Fig 12. Azure Ao Absorbance Microplate Reader User Manual Page 18...
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Page 19: Shake Setting
1. On “Programs” interface, click “Shake” button under “Shake parameters” to set shake speed and time. Figure 13 2. Click button beside “Shake” to choose “On” or “Off”. See Fig 14. Figure 14 Azure Ao Absorbance Microplate Reader User Manual Page 19... -
Page 20: Calculate
1. On “Programs” interface, click box to the right of ”Calculate” to set “Calculate parameters”. Click button beside “Kinetic” to choose On or Off. Set parameters for kinetic reads when set to “On.” See Fig 17. Azure Ao Absorbance Microplate Reader User Manual Page 20... - Page 21 Change: Set change value (if the absorbance value set is less than the first reading, it will not be recorded.) b. First readings: Beginning reading and recording data points. Last readings: End of reading and recording data points. Azure Ao Absorbance Microplate Reader User Manual Page 21...
- Page 22 M1-M2,M1/M2,M1+M2,M1*M2,M2-M1,M2/M1 (M1,M2 refers to absorbance value for wavelength 1 and wavelength 2). Curve fitting In the “Calculate parameters” interface, Click button beside “Curves”. Interface will appear as in Figure 21. Figure 21 Azure Ao Absorbance Microplate Reader User Manual Page 22...
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Page 23: Interpret
5. Click “OK” to complete and return to main interface. 6.1.6—Quality Controls 1. In “Programs” interface, press area to the right of “Quality”. “Quality controls” interface will appear as in figure 23, below. Azure Ao Absorbance Microplate Reader User Manual Page 23... - Page 24 QC concentration or ABS. Set Concentration value, ABS and SD according to reagent user manual. 5. When using Quality controls, set up the QC sample on the Layout interface (refer to “Layout” section above). Azure Ao Absorbance Microplate Reader User Manual Page 24...
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Page 25: Report
Connect USB drive using USB port on the right side. Note: “Export” has same function on each data interface. Interpret analysis data Click to show the most recent interpreted result. Figure 26 Azure Ao Absorbance Microplate Reader User Manual Page 25... - Page 26 “Curve” interface). Axes Options: Linear/Linear (Linear is ABS and corresponding concentration), Linear/Log, (Linear is the ABS and corresponding log value of the concentration), Log/Linear, Log/Log, Log/Linear. Figure 28 Azure Ao Absorbance Microplate Reader User Manual Page 26...
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Page 27: Kinetics Report Interface
Click this button and the input cycle number to change and verify. Kinetics measurement Analysis indicates the data processing method for kinetic measurement. (See section 6.2.2 Kinetics setting). Azure Ao Absorbance Microplate Reader User Manual Page 27... - Page 28 At the A1 position click to see the absorbance change of each sample position. See Fig 33. You can input the sample position No. to check curve. Figure 32 Figure 33 Azure Ao Absorbance Microplate Reader User Manual Page 28...
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Page 29: Records
Results Interface will save data to a USB in .CSV format. 6.4—Settings This section introduces settings for language, filter, maintenance, print, date and time. Click Setting at the upper right of the main interface. Azure Ao Absorbance Microplate Reader User Manual Page 29... -
Page 30: Language Setting
Press ”Language” icon in the setting interface. See Fig 36. Figure 36 Click button right beside Language. See Fig 37. Select language needed and press OK. Currently English and Chinese are available. Figure 37 Azure Ao Absorbance Microplate Reader User Manual Page 30... -
Page 31: Filter Setting
Click the blank position No. and input the wavelength. The default unit is nm. See Fig 39. Figure 39 Note: The position numbers in software and filter wheel should be the same, or measurements will be incorrect or instrument may error. Click OK when settings are complete. Azure Ao Absorbance Microplate Reader User Manual Page 31... -
Page 32: Maintenance
Press “OK” to return to main interface. 6.4.4—Time and Date Setting Click “Date & time” to set. Format of Date is Month/Day/Year, with Time as Hour:Minute:Second. See Fig 41. Figure 41 Azure Ao Absorbance Microplate Reader User Manual Page 32... - Page 33 To change the date and time, click the setting area as shown in Fig 42. Figure 42 Click “OK” to complete setting. Azure Ao Absorbance Microplate Reader User Manual Page 33...
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Page 34: Maintenance, Storage & Transportation
Make sure instrument is flat and stable, then attach the plate. See Fig 44. Note: Do not touch the surface of the lamp. Hold the lamp base when replacing and apply even force to avoid damage. Figure 43 Azure Ao Absorbance Microplate Reader User Manual Page 34... -
Page 35: Add Optical Filter
• Avoid touching filter surface to prevent contamination that can lead to problems with precision. • Don’t touch any other mechanical or electric part when installing the filter wheel. Figure 45 Azure Ao Absorbance Microplate Reader User Manual Page 35... -
Page 36: Storage And Transportation
Figure 46 7.2—Storage and transportation 1. Store at room temperature (-10°C~40°C), with relative humidity less than 80%, no corrosive gas, and good ventilation. 2. Avoid heavy shock, vibration, and humidity during transportation. Azure Ao Absorbance Microplate Reader User Manual Page 36... -
Page 37: Troubleshooting
Check that microplate is placed level in drawer Start measurement is interrupted Communication interruption Press “Start/Stop” to restart measurement Strong light No filter in the selected Delete the empty filter setting filter position Azure Ao Absorbance Microplate Reader User Manual Page 37... - Page 38 • info@azurebiosystems.com Copyright © 2020 Azure Biosystems. All rights reserved. The Azure Biosystems logo and Azure Biosystems is a trademark of the ® Company. All other trademarks, service marks and trade names appearing in this brochure are the property of their respective owners.
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