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Jasco PU-1580 Operation Manual

Intelligent hplc pump
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MODEL PU-1580
Intelligent HPLC Pump
Operation Manual
P/N: 0302-0375B

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Summary of Contents for Jasco PU-1580

  • Page 1   MODEL PU-1580 Intelligent HPLC Pump Operation Manual P/N: 0302-0375B...
  • Page 2   Safety Considerations To ensure operation safety, this instrument must be operated correctly and maintained regularly according to schedule. Carefully read to fully understand all safety precautions in this manual before operating the instrument. This manual denotes precautions against actions that can result in hazardous situations or equipment damage by using the signal words WARNING, CAUTION, and Note.
  • Page 3 If a warning label peels off the instrument or becomes illegible, contact your local JASCO distributor (see list on the back cover of this manual) for a replacement label. Be sure to indicate the part number (P/N) on the label.

  • Page 4: Limited Warranty

      Limited Warranty Products sold by JASCO, unless otherwise specified, are warranted for a period of one year from the date of shipment to be free of defects in materials and workmanship. If any defects in the product are found during this warranty period, JASCO will repair or replace the defective part(s) or product free of charge.

  • Page 5: Regulatory Statements

    1990 + Amd.1:1992 + Amd.2: 1995----Safety requirements for electrical equipment for measurement, control and laboratory use. • A “Declaration of Conformity” in accordance with the above standards has been made and is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (Lc), Italy.

  • Page 6: Preface

    When not using the instrument, keep this manual stored in a safe place. Should this operations manual be lost, order a replacement from your local JASCO distributor. Installation Conditions To ensure operation safety, observe the following conditions: Do not operate the instrument under voltage fluctuations exceeding 10% of the recommended line voltage.

  • Page 7: Maintenance

    JASCO distributor before moving the instrument to another location. Consumable parts should be ordered according to part number from your local JASCO distributor. If a part number is unknown, give your JASCO distributor the model name and serial number of your instrument.

  • Page 8: Table Of Contents

      TABLE OF CONTENTS Safety Considerations................i Limited Warranty..................iii Regulatory Statements ................iv Preface ....................v Maintenance ...................vi TABLE OF CONTENTS................. vii Product Description and Specifications..........1 1.1. Product Description ..................1 1.2. Specifications ....................1 Part Names and Descriptions ............4 2.1.
  • Page 9   5.6.2. Editing time programs ................28 5.6.3. Example of a low-pressure gradient program........... 31 5.7. High- or low-pressure gradient operations............. 32 5.7.1. High- or low-pressure gradient program execution........32 5.7.2. Parameter changes while the gradient program is running ...... 33 5.7.3.

  • Page 10: Product Description And Specifications

    1. Product Description and Specifications 1.1. Product Description The Model PU-1580 Intelligent Pump is a high performance, high functionality pump for HPLC. The pump provides extremely consistent solvent delivery via SSQD (Slow Suction Quick Delivery). The pump may be operated in normal operation mode or program mode. Normal operation mode offers a constant flow rate or pressure.
  • Page 11 Programming of flow rate, composition ratio, maximum pressure, minimum pressure, event relay signals (three circuits), and solvent switching (64 steps, 10 files) is possible High pressure gradient: 2-solvent high-pressure (mixing) method using two PU-1580 units Low pressure gradient: 3-solvent low pressure (mixing) method using a low-pressure gradient unit (LG-1580-02) Composition ratio setting: 0 ~ 100%, 0.1% increments...
  • Page 12 Composition ratio setting range: 0 ~ 100% Diagnostic functions: Items checked: Memory (ROM, RAM), pressure sensors, DC power, memory backup Maintenance program: Flow rate correction function, pressure sensor inspection functions Operating temperature range: 10 ~ 40°C Additional specifications: Wetted materials: Sapphire, ceramics, stainless steel (SUS 316), fluoride resin, PEEK Pressure sensors:...

  • Page 13: Part Names And Descriptions

    2. Part Names and Descriptions 2.1. Front Panel LCD Panel Control Keys PU-1580 MODE FLOW TIMER ml/min PRGM CF/CP MONIT PUMP PRESS P.MAX P.MIN Pump Head Editing Keys SHIFT VALVE PRGM Drain Tube P.MAX VALVE P.MIN Purge Valve COMP FLOW...

  • Page 14: Operation Panel

    2.2. Operation Panel MODE FLOW TIMER ml/min PRGM CF/CP MONIT PUMP PRESS P.MAX P.MIN kg/cm SHIFT VALVE PRGM P.MAX VALVE P.MIN COMP FLOW EVENT CLEAR EDIT/ENTER Figure 2.2 Operation Panel Function [PUMP] Used to start/stop solvent delivery. The pilot lamp is lit when the pump is on.

  • Page 15: Rear Panel

    [VALVE/4] Used to specify the solvent selection unit in normal operation mode and time program mode. [PMAX/5] Used to specify the maximum pressure in normal operation mode and time program mode. [PMIN/6] Used to specify the minimum pressure in normal operation mode and time program mode.
  • Page 16 Part Function Low-Pressure Gradient Unit Accepts a cable from the low-pressure gradient unit Connector (LG-1580-02). Solvent Selection Unit Connector Accepts a cable from the 6-solvent selector valve unit (LV-1580-03). Event Relay Output Connector Connects with external equipment (i.e., a 2-column switching valve unit).

  • Page 17: Right Side Panel

    2.4. Right Side Panel Pressed-in nuts for securing the injector holder Pressed-in nuts for securing the stack band Figure 2.4 Right Side Panel...

  • Page 18: Bottom Panel

    2.5. Bottom Panel Fixing screws for shipping Rubber feet Solvent drain port Vat post Figure 2.5 Bottom Panel Part Function Solvent drain port Allows mobile phase solvent or plunger washing solvent (water) leaks to drain. Vat post Secures the receiving vat. Fixing screws Fix internal moving parts to prevent damage during shipping.

  • Page 19: Power-On And Power-Off

    Note 1: If a ROM, RAM, or DC power error occurs, turn the power off and then on again. If the error reoccurs, contact your local JASCO distributor. Note 2: If a C-MOS RAM, or pressure sensor error occurs, follow the instructions provided in the maintenance manual.

  • Page 20: Power-Off

    Note 1: Although the pressure is given in MPa (as shown on the display panel), the pressure can also be given in MPa (megapascals) (refer to Section 3.2). In this operations manual MPa will be used when describing pressure. Note 2: The pump OFF timer is used to automatically turn off the pump. The time countdown begins from the moment the timer is set and the pump stops when the timer reaches 0.

  • Page 21: Operations In Normal Operation Mode

    4. Operations in Normal Operation Mode Delivery with a constant flow rate or constant pressure is performed in normal operation mode. 4.1. Switching Between Constant Flow Mode and Constant Pressure Mode The delivery mode is changed using the [CF/CP] key. The screen changes, as shown in Fig.
  • Page 22 Note 2: The number keys are integrated with the parameter keys. When a value is flashing the numbers can be used. (2) Changing the maximum and minimum pressure settings Delivery automatically stops when the pressure exceeds the maximum pressure setting or drops below the minimum pressure setting for two minutes.

  • Page 23: Constant Flow Rate Start And Stop

    (4) Changing the solvent selection unit setting The solvent selection unit enables solvent exchange for up to six solvents. Input range: 1 ~ 6 Constant flow monitor screen 1.000 (The solvent selection unit setting 10.0 cannot be checked using this screen.) Press [VALV/4] Press [3][EDIT/ENTER] to input 3 EVENT:...

  • Page 24: Error Messages During Pumping

    (2) Stop Press the [PUMP] key to stop the pump during pumping. The key lamp will be turned off. Note 1: The pump can be started and stopped automatically using timers (see Section 6.1 Timer Functions) Note 2: Parameters can be changed during pump operation. The new parameters will be valid immediately after being set.

  • Page 25: Constant Pressure Start And Stop

    (1) Changing the pressure setting Input range: 0 ~ 500 kgf/cm (in 1kgf/cm increments) Constant pressure monitor screen 5.0(10.0) MPa ((100) is the new setting.) PRESS 0. 0 MPa Press [EDIT/ENTER] Press [1][0][0][EDIT/ENTER] to input 100 Pressure flashes 5.0 MPa Pressure input screen PRESS 0.0 MPa...

  • Page 26: Operations In Program Mode

    5. Operations in Program Mode 5.1. Description In the program mode, gradient delivery and time programs for flow rate, pressure, solvent selection unit, and event relays can be performed. Time programs can contain up to 64 steps, and up to 10 program files can be stored in the memory of the instrument. Gradient delivery can be performed using either the high-pressure (mixing) method or the low-pressure (mixing) method.

  • Page 27: Setting The File Number (Loading Files)

    Refer to Section 5.5.1 for information regarding low- pressure gradient mode Edit the time program Refer to Section 5.4.2 for information regarding high- pressure gradient mode Refer to Section 5.5.2 for information regarding low- pressure gradient mode Execute Refer to Section 5.6 5.2.

  • Page 28: Gradient Mode Confirmation

    Normal operation mode 1.000 [CF/CP] 5.0 MPa 10.0 PRESS 0. 0 MPa [PRGM] Program operation mode screen [MONIT] HPG 2 T: 0.0 PRG 2 1.000 A 50.0 E000 V2 10.0 Figure 5.2 Switching Between Normal Operation Mode and Program Mode Note: Switching between normal operation mode and program mode can only be performed for Pump 1.

  • Page 29: Editing A Program In High-Pressure Gradient Mode

    Note: When the mode does not match the system being used, refer to Section 6.4 to change the mode. 5.5. Editing a Program in High-pressure Gradient Mode 5.5.1. Editing the initial conditions The flow rate, maximum pressure, minimum pressure, event relay output, solvent selection unit, and solvent composition are set as initial conditions.

  • Page 30: Editing Time Programs

    [MONIT] HPG 2 T: 0.0 PRG 2 1.000 A 50.0% E000 10.0 or [MONIT] ST 0 INITIAL Step 0 (Initial conditions screen) Composition ratio A 50.0% [xx] [EDIT/ENTER] [EDIT/ENTER] (Input a composition ratio) ST 0 INITIAL Composition ratio flashes A 50.0% (Awaiting input for A) Figure 5.6 Initial Conditions Screen 5.5.2.
  • Page 31 ST 0 INITIAL Step 0 (Initial conditions screen) A 50.0% ▼ ▲ ▼ ST 2 T:0.0 ST 1 T:0.0 Step 2 screen Step 1 screen [EDIT/ENTER] [EDIT/ENTER] ST 1 T:0.0 ST 2 T:0.0 Time flashes Time flashes (awaiting input) (awaiting input) Press [1][EDIT/ENTER] Press [2][EDIT/ENTER] to input 1 minute...
  • Page 32 After Step 1 has been programmed, press the [τ] key to advance to the next step. Repeat steps 1) through 5) to complete the time program. Table 5.1 lists the functions that can be used in time programs and the corresponding keys used to designate each of the functions.
  • Page 33 2) Skip correct settings by pressing [EDIT/ENTER] until the desired input screen appears. 3) Enter the correct setting. For example, when changing only the gradient composition ratio, perform the operations shown in Fig. 5.8. However, if settings are changed starting from the time setting, the function selection screen, shown on the right side of Fig.

  • Page 34: Example Of A High-Pressure Gradient Program

    5.5.2.4 Inserting steps into a time program This section describes how to insert a single step into a time program contained in a file. 1) Press [SHIFT][▼] to advance to the final step in the program. 2) Press [▼] to advance to a blank step. 3) Enter values according to the instructions provided in Section 5.5.2.1.

  • Page 35: Low-Pressure Gradient Mode

    Initial conditions: 1.000 ST 0 INITIAL Flow rate: 1 ml/min Maximum pressure: 10.0 MPa 10.0 A 50.0% Composition ratio: A 50% (B 50%) A: CH CN B: H O ▼ ▲ ST 1 T:20.0 Composition ratio after 20 minutes will be A: 100% and ▼...
  • Page 36 Note: When HPG is displayed in the program monitor screen, refer to Section 6.4 to change to the low-pressure gradient mode (LPG). (1) Flow rate, maximum pressure, minimum pressure, event relay output, and solvent selection unit changes: Parameters are changed in the same way that normal operation mode parameters are changed.

  • Page 37: Editing Time Programs

    5.6.2. Editing time programs 5.6.2.1 Time program input (1) Inputting an example program: As an example, a time program in which the initial conditions (solvent compositions of A=50%, B=50% and a flow rate of 1 ml/min) are changed to have a solvent composition of A=1.0%, B=99.0% (and C=0%) after 1 minute and then changed again to have a flow rate of 2 ml/min after 2 minutes.
  • Page 38 (2) Time program input method: The input sequence is as follows: time, function, value relative to the function. 1) From the initial conditions screen (Step 0 screen), press the [▼] key to advance to the Step 1 screen. 2) Press [EDIT/ENTER] (time flashes). 3) Enter the time in minutes (minimum increment: 0.1 min).
  • Page 39 (3) Step advance: [▼]: Advances to the next step [▲]: Returns to the previous step [SHIFT][▼]: Moves to the last step in the program [SHIFT][▲]: Moves to step 1 5.6.2.2 Correcting time programs When times and functions are incorrect: 1) Use the [▼] key to advance to the incorrect step. 2) Enter the correct settings, starting with time (Refer to Section 5.5.2.1).

  • Page 40: Example Of A Low-Pressure Gradient Program

    5.6.2.3 Deleting individual steps or several steps from a time program Deleting individual steps: 1) Press the [▼] key to advance to the step to be deleted. 2) Set the time in the step to 0 ([EDIT/ENTER][0][EDIT/ENTER]). 3) Press the [MONIT] key to return to the monitor screen. Steps will be rearranged in sequence and the selected step will be deleted.

  • Page 41: High- Or Low-Pressure Gradient Operations

    In the following example the solvent that increases as the gradient advances (acetonitrile) will be defined as A. A signal proportional to the composition of A will be output from the COMP output terminal on the back panel. An example program is described in Fig. 5.16. Initial conditions ST 0 INITIAL...

  • Page 42: Parameter Changes While The Gradient Program Is Running

    (1) Pumping under initial conditions: When solvent preparation and other work related to the analysis is complete, press the [PRGM RUN] key. Pumping will begin under the initial conditions. Wait for the baseline to stabilize. The appearance of the key lamps during pumping under initial conditions are described below: •[PUMP] key: Lamp is lit during pumping •[PRGM RUN] key: Lamp flashes during pumping under initial conditions...

  • Page 43: Operation In Conjunction With An Autosampler

    [MONIT] HPG 2 T: 0.0 PRG 2 1.000 A 50.0 E000 V2 10.0 Time and composition monitor screen Flow rate and pressure monitor screen [SHIFT] [COMP 1] [MONIT] PUMP OFF TIME 2.0 HOUR Pump OFF timer screen [VALV 4] [EVEN 3] EVENT: VALVE: Event relay output and solvent selection unit setting screen...
  • Page 44 Composition ratio Time "STATUS" FLUSH WAIT LOAD READY LOAD FLUSH WAIT LOAD FLUSH WAIT LOAD INJECT INJECT INJECT Autosampler start Figure 5.19 Autosampler Status and Gradient Program Progression Initially, the pump remains under the initial conditions until the first injection by the autosampler, after which, the program starts.

  • Page 45: Special Operations

    6. Special Operations Operations accessed using the [SHIFT] key are explained in this section. With the exception of the timer functions, these operations generally need only be performed once, because the settings are stored in CMOS-RAM and thus do not need to be reentered each time the power is turned on.

  • Page 46: On Timer Setting ([Shift][2])

    PUMP STOP!! TIMER OFF Figure 6.2 Pump Stop Screen Press the [CLEAR] key to return to the monitor screen. The timer will be OFF in the monitor screen. Turning the OFF timer off: Repeat the operations in described in (1) and set the time to 0. The timer will be turned OFF.

  • Page 47: Flow Rate Correction ([Shift][3])

    When the ON timer is set, the [PUMP] key lamp will flash regardless of the current pumping status and the time countdown will begin. Turning the ON timer off: Reset the ON timer to 0. Note: The timer is automatically set to OFF when the power is turned ON. 6.2.

  • Page 48: Switching Between High- And Low-Pressure Gradient Modes ([Shift][6])

    1.000 10.0 [SHIFT] [4] [MONIT] [EDIT/ENTER] PUMP PUMP [x][EDIT/ENTER] Flashes Pump number screen Pump number input screen Figure 6.4 Pump Number Setting Screen 6.4. Switching between high- and low-pressure gradient modes ([SHIFT][6]) This function is used to switch between high-pressure and low-pressure gradient modes.

  • Page 49: Pressure Sensor Signal ([Shift][6])

    Press the [PRGM] key while the normal operation mode monitor screen is displayed. One of the following screens will appear. The monitor screen will not change, 1.000 but the [PUMP] key lamp will flash when the ON timer is set. 10.0 [SHIFT] [2] [MONIT]...

  • Page 50: Adjustment Of Lpg Comp [Shift][7]

    6.6. Adjustment of LPG COMP [SHIFT][7] The LPG COMP parameter adjusts the timing of the proportioning valves of the LG-1580-02 Low-pressure Mixing Gradient Unit. This function should be used when a more accurate solvent composition with specific solvents is required. 6.6.1.

  • Page 51: Using The Optional Semi-Micro Kit ([Shift][.])

    =(0.292 – 0.040)/(0.640-0.040) =0.42 = 42% 9) As in the above example, the composition becomes greater than the set value by 5%, the accurate LPG COMP value is smaller than the present value by the same factor (5%). If the present LPG COMP value is 1.000, the recalibrated value becomes 0.950.
  • Page 52 Value: 10 digits CF 1.000 0 .0 10.0 [MONIT] [SHIFT][MONIT] [EDIT/ENTER] SET SERIAL NO. SET SERIAL NO. XXXXXXXXXX XXXXXXXXXX [X][EDIT/ENTER] Figure 6.11 Registering the Serial Number. For replacement parts or questions regarding maintenance: Contact your nearest JASCO representative or service center.

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