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Summary of Contents for Histo-Line Laboratories MC5050
- Page 1 MC5050 Cryostat User’s Manual Histo-Line Laboratories s.r.l. Applicable Model: Viale G. di Vittorio, 30 20048 Pantigliate (MI), Italy MC5050 Tel. +39 02.55230061 Fax: +39 02 55213764 www.histoline.com email: histoline@histoline.com Revised Version V4.8-English Version-Mar 2023...
- Page 2 Before use, be sure to read the User Manual carefully and follow the instructions correctly to the user manual. In addition, if you have any questions during use, contact technical support, we will be glad at your service. Histo-Line Laboratories s.r.l. V4.8...
- Page 3 Histo-Line Laboratories s.r.l. reserves the right to make changes to the manufacturing process subject to regulatory requirements without prior notice. In this way we can continuously improve the technology and manufacturing techniques used in our products.
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Page 4: Table Of Contents
5.1.2 Slice and Retouch Setting ..................25 5.1.3 Cold Stage Temperature Setting / Semiconductor Cooling Function ..... 25 5.1.4 Lighting and UV Lamp ..................26 5.1.5 Drying Button ......................26 5.1.6 Fault reporting function ..................27 5. 2 Button Panel ........................28 Histo-Line Laboratories s.r.l. V4.8... - Page 5 9. Section Tissue Temperature Selection Table ................47 10. Warranty and Service ......................... 49 10.1 Warranty .......................... 49 10.2 Technical Service Information ..................49 10.3 Scrapping and Disposal ....................49 11 Electromagnetic Compatibility Indicators ................. 49 Histo-Line Laboratories s.r.l. V4.8...
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Page 6: Information
This warning symbol indicates the instrument surface that will be hot during operation. Avoid direct contact to prevent burns. Environmental protection sign of SJ/T 11364. The figure in the symbol refers to the product’s number of years of “environment-friendly use”. Serial number Histo-Line Laboratories s.r.l. V4.8... - Page 7 The tilt indicator provides clear visual indication if a shipment has been transfered and stored vertically according to your requirements.In the case when the shipment is tilted for 60° or over, the blue quartz sands flow into the arrow-shape window and remain visible in the window permanently. Histo-Line Laboratories s.r.l. V4.8...
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Page 8: Instrument Use
Freezer sections: quickly obtaining low temperature and slicing. Refrigeration parts: including refrigeration compressor and semiconductor cooling fin. The MC5050 Semi-automatic Cryostat Microtome is used for rapid freezing of pathological sections of human and animal body tissue. It can be widely used for preparing thin sections of samples of varying hardness for use in pathological diagnosis, analysis and research in hospitals, medical colleges by legal medical experts and propagation institutes. -
Page 9: Safety
The performance indicators of the instrument are in line with Histo-Line's technical requirements of the cryostat. In order to maintain this condition and ensure safe operation, the user must comply with all safety precautions and warnings in this User Manual. Histo-Line Laboratories s.r.l. V4.8... -
Page 10: Warning
Failure to follow these instructions may result in accidents, personal injuries, and damages to the instrument or accessories. This warning label is used on the surface of the instrument that will be hot during operation. Histo-Line Laboratories s.r.l. V4.8... -
Page 11: Warning - Transport And Installation
Do not touch falling accessories – there is a risk of injury! ⚫ Lock the hand wheel before cleaning! ⚫ Do not use any solvents containing acetone or xylene for cleaning! ⚫ Ensure that liquid does not enter the instrument during the cleaning process! Histo-Line Laboratories s.r.l. V4.8... -
Page 12: Equipment Placement Requirements
After the machine is stopped, the glass door should be opened to allow the water vapor to evaporate as soon as possible to keep the cooling chamber dry. After the UV lamp is damaged, it needs to be replaced as it is the same brand, Histo-Line Laboratories s.r.l. V4.8... - Page 13 The cooling chamber should be cleaned frequently and kept clean, but especially pay attention that not to be scratched by the blade. Never use rapid freeze spray in the cryochamber. Histo-Line Laboratories s.r.l. V4.8...
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Page 14: Instrument Components And Specifications
3. Instrument Components and Specifications Overview Figure 2 Front picture of MC5050 Cryostat 1 Display 2 Keyboard 3 Waste barrel 4 Foot cup 5 Power switch 6 Glass door 7 Hand wheel Figure 3 The picture cooling chamber 8 Sample disk... -
Page 15: Technical Parameters
20μm～50μm, with a step of 2μm 50μm～100μm, with a step of 5μm Section thickness deviation ±20% 1μm～600μm Trimming thickness range 1μm～50μm, with a step of 5μm Trimming step amount 50μm～100μm, with a step of 10μm 100μm～600μm, with a step of 50μm Histo-Line Laboratories s.r.l. V4.8... - Page 16 Minimum reaching temperature -60℃（+5℃） of semiconductor rapid cooling area Number of common freezing points Number of semiconductor fast cooling points Refrigerant R449A/400g±5g Cooling capacity 690W Refrigerant and compressor oil should only be replaced by qualified authorized service personnel! Histo-Line Laboratories s.r.l. V4.8...
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Page 17: First Installation
If the above situation occurs, mark it on the delivery documents and check whether the goods are damaged. Unpacking Tools Figure 4 Tools for unpacking 1. Electric screwdriver 2. Adjustable wrench 3. Utility knife 4. Allen wrench Histo-Line Laboratories s.r.l. V4.8... -
Page 18: Unpacking
Check the received parts according to the attached packing list to confirm whether the delivery is complete! If any discrepancies are found, contact Histo-Line or your local distributor immediately. To prevent damage to the instrument or samples, only accessories and spare parts authorized by Histo-Line can be used. Histo-Line Laboratories s.r.l. V4.8... -
Page 19: Accessory Installation
The hand wheel can only be locked at the highest and lowest position and the rest of the position cannot be locked. Hand wheel unlock: reversely push the hand wheel lock lever. Histo-Line Laboratories s.r.l. V4.8... -
Page 20: Hand Wheel Block Installation
When the top cover is locked, it is also tightened for 5~8 cycles. Make sure that the screw can support the top cover properly. Continue to tighten the screw, which will cause the top cover to deform or even rupture. Histo-Line Laboratories s.r.l. V4.8... -
Page 21: Fixed Card Core
EVA cotton. The chuck core can move up and down normally. 4.4.5 Waste Trough Installation Figure 10 The picture of waste trough Place the waste trough in the body of the universal specimen clamp in the direction of the illustration (Figure 10) Histo-Line Laboratories s.r.l. V4.8... -
Page 22: Blade Holder Installation
(Figure 11-2) Slide to the appropriate position, then rotate the handle to lock the blade holder. 4.4.7 Glass Door Installation Conductive sheet Conductive electrode Figure 12 The procedure of glass door installation Histo-Line Laboratories s.r.l. V4.8... -
Page 23: Power Supply Cable Connection
Check that the tool holder is installed in place and locked on the tool holder mounting plate of the freezer compartment. Put the waste slot, brush, No. 4 hook pen, front brush holder, blade and other utensils into the freezer compartment for pre-cooling. Close the glass door. Histo-Line Laboratories s.r.l. V4.8... -
Page 24: Operation
(When the compressor stops for less than 3 minutes and receives the signal to start the compressor again, the compressor will not start immediately, it will be delayed until the stop time reaches 3 minutes before starting). Histo-Line Laboratories s.r.l. V4.8... -
Page 25: Slice And Retouch Setting
600s, and the countdown is automatic. When the value is 0, the semiconductor cooling is automatically turned off , and the icon is touched before turn-off, and the semiconductor cooling function is closed. Histo-Line Laboratories s.r.l. V4.8... -
Page 26: Lighting And Uv Lamp
"drying" function is running, the program will automatically end the drying mode and return to normal cooling mode; 2: Before "drying" is turned on, all samples in the freezer must be emptied; Histo-Line Laboratories s.r.l. V4.8... -
Page 27: Fault Reporting Function
Click the icon to see the error reporting details page in Figure 18. Figure 18 Fault detail interface Attention: 1. Click the back button on the fault detail interface to return to function interface and the red error icon will disappear. Histo-Line Laboratories s.r.l. V4.8... -
Page 28: Button Panel
"Back" direction The card head core moves forward quickly, and the position icon moves quickly to the "Front" direction Chuck core position indicator icon Figure 19 The picture of keyboard interface Histo-Line Laboratories s.r.l. V4.8... -
Page 29: Function Setting And Introduction
3. Indication 3 is starting defrost time. Click the time black box to display Choosing the hour and minute. Press the number on the bottom right to increase and decrease the time. Press the “Save” button to save the settings Histo-Line Laboratories s.r.l. V4.8... -
Page 30: Hibernation Setting
“Cancel” button to abandon the settings. Precautions: 1: When the sleeping time ends, the device enters the normal working state, the device automatically starts the compressor, and cools to the specified box temperature and station Histo-Line Laboratories s.r.l. V4.8... -
Page 31: Disinfection Setting
5.3.4 Time reset in the function interface Double-click the icon "Reset" in the lower left corner of the function interface, and the date and time settings of the entire function interface will be automatically restored to the factory Histo-Line Laboratories s.r.l. V4.8... -
Page 32: Compressor Overheat Protection Function
5.5.2 Slice Retraction Value Setting Press the button“+” and “-” to increase or decrease the retraction stroke. The retraction stroke range is 0--100um. Please click "Save" to save retraction value changes. The specimen head feed Histo-Line Laboratories s.r.l. V4.8... -
Page 33: System Language Setting
5.5.4 UV lamp service time display Displays the actual service life of the UV lamp. The user will be prompted to replace the UV lamp when the service life of the UV lamp exceeds 6000 hours. Histo-Line Laboratories s.r.l. V4.8... -
Page 34: Blade Holder Module
7. Blade pressing plate 8. Anti-roll plate’s flip handle 3. Blade clamping handle 4. Holder locking handle for 9. Anti-freeze hand rubber stopper left-and-right movement 5. Holder locking handle for 10. Blade slicing angle locking screw back-and-forth movement Histo-Line Laboratories s.r.l. V4.8... -
Page 35: Blade Holder Front And Rear Position Adjustment
The microtome blade is extremely sharp! Handle with care! Do not catch the falling blade! Histo-Line Laboratories s.r.l. V4.8... -
Page 36: Anti-Roll Plate Adjustment
The adjustable angle range is 0-10°, then fix the blade slicing angle locking screw. If the slicing effect is not good, the angle can be tested by 1° until it is adjusted well, but the angle cannot be changed more than 10°. Histo-Line Laboratories s.r.l. V4.8... -
Page 37: Sample Disk Installation
It is locked to the front of the card core. Loosen the head adjustment lock screw on the core of the chuck, and move the head adjustment handle to adjust the direction of the surface of the specimen, tighten it after selection. Histo-Line Laboratories s.r.l. V4.8... -
Page 38: Specimen Cryoforming
During specimen freezing, a heavy hammer can be placed on the specimen surface to accelerate the freezing of the specimen surface and flatten the specimen surface; After the specimen is frozen, insert the carrier into the core of the chuck. Once it is tightened, start slicing. Histo-Line Laboratories s.r.l. V4.8... -
Page 39: Sectioning Operation
Adjust to the trimming mode, press the fast forward and slow forward keys to move the specimen closer to the blade, and turn the handwheel to trim. The thickness of the trimming is recommended to be 20 ~ 30μm; Histo-Line Laboratories s.r.l. V4.8... -
Page 40: Sectioning
4. The frozen section is opposite to the general paraffin section. The frozen section does not cut the long side of the tissue. It is best to cut the short side of the tissue, that is, the contact line between the tissue and the slicing blade is shorter. Histo-Line Laboratories s.r.l. V4.8... -
Page 41: Cleaning And Maintenance
Remove the blade from the blade holder, then put it back to the cartridge. Remove the blade holder to clean up. Remove the specimen from the specimen clamp. Use a dry brush to remove the section waste. Histo-Line Laboratories s.r.l. V4.8... -
Page 42: Cleaning The Instrument Enclosure
Clean the condenser as needed Open the condenser sealing baffle on the right side of the fuselage, and use a brush to clean the dust on the condenser fins, or spray with high-pressure airflow. Histo-Line Laboratories s.r.l. V4.8... -
Page 43: Take The Slicing Core From The Freezer
When reinstalling the slicer movement, the inside of the movement must be completely dry. Otherwise, the moisture inside the movement will freeze at low temperatures, which will damage the body. Any items taken from the freezer must be thoroughly dried before being put back. Histo-Line Laboratories s.r.l. V4.8... -
Page 44: Replacing Component
If the UV lamp is broken, it must be replaced by a professional. 7.7 Use of Waste Tank The waste liquid tank is used to collect defrosting and defrosting liquid, tissue thawing liquid, Histo-Line Laboratories s.r.l. V4.8... -
Page 45: Faq And Handling
Blade is not fully locked Lock the "blade lock handle"; lines, the section is Foreign matter is attached to Clean the knife surface of the incomplete and breaks the blade installation groove, knife plate and the knife Histo-Line Laboratories s.r.l. V4.8... - Page 46 Foreign matter on the edge of Clean the anti-roll plate and the anti-roll plate remove foreign objects When opening the anti-roll Cutting angle is too small Adjust the angle of blade a plate, the slice is curled little larger Histo-Line Laboratories s.r.l. V4.8...
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Page 47: Section Tissue Temperature Selection Table
Tissue freezing is too slow semiconductor refrigeration points to freeze tissues; 2. Use heavy hammer correctly to speed up tissue freezing; 9. Section Tissue Temperature Selection Table Tissue Chamber temperature Adipose tissue -30℃~-35℃ Histo-Line Laboratories s.r.l. V4.8... - Page 48 -15℃~-20℃ Uterine scraping -10℃~-15℃ Cartilage -10℃~-15℃ Caution: The temperature given in the above table is a summary of long-term experience, but these are only approximate values, because specific adjustments may be required for actual organizations. Histo-Line Laboratories s.r.l. V4.8...
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Page 49: Warranty And Service
The instrument or its components must be disposed of in accordance with local regulations. 11 Electromagnetic Compatibility Indicators Note: [MC5050] [Cryostat microtome] shall meet the emission and immunity requirements specified in IEC61326-2-6, as shown in the table below. The user shall be responsible for ensuring the electromagnetic compatibility of the environment for the equipment to work normally. - Page 50 Warning: [MC5050] [Cryostat microtome] is designed and tested according to Class A equipment in CISPR 11. In the home environment, the equipment may cause radio interference, so that protective measures should be taken. It is prohibited to use the equipment next to an intense radiation source (such as an unshielded RF source), otherwise the normal operation of the equipment will be affected.
- Page 51 [Manufacturer Name] Histo-Line Laboratories s.r.l. [Address] Pantigliate (MI) - 20048 - Viale G. Di Vittorio, 30 - Italy. [Tel] +39 02 55230061 [Fax] +39 02 55213764 [Info] histoline@histoline.com [Customer Service] techservice@histoline.com [Website] www.histoline.com...
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