Hamamatsu ORCA-Flash4.0 LT3 Instruction Manual

Digital cmos camera

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ORCA-Flash4.0 LT3

Digital CMOS camera

C11440-42U40

Instruction manual

Thank you for your purchase

•

Follow the safety precautions in Chapter 1 in order to avoid personal

injury and damage to property when using this camera. The manual

describes the correct handling method of the camera and provides

instructions that should be followed to avoid accidents. Read this manual

carefully before using this camera. After reading this manual, store it in a

location where you can refer to it at any time.

Ver. 1.1

Dec. 2022

HAMAMATSU PHOTONICS K.K.

B6020301-01

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Summary of Contents for Hamamatsu ORCA-Flash4.0 LT3

Page 1 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual Thank you for your purchase • Follow the safety precautions in Chapter 1 in order to avoid personal injury and damage to property when using this camera. The manual describes the correct handling method of the camera and provides instructions that should be followed to avoid accidents.
Page 2 ~ Blank page ~...
Page 3: Safety Precautions
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 1. SAFETY PRECAUTIONS SYMBOLS The following symbols can be found on this camera: Direct current Alternating current CLASSIFICATION OF WARNINGS We have classified the warnings symbols that appear in this instruction manual and on the camera as follows for your convenience.
Page 4 Such as the image suddenly disappearing or the occurrence of a strange noise, a strange smell or smoke coming from the camera, immediately turn off the power switch and unplug the power supply cord and contact Hamamatsu subsidiary or your local distributor. Do not attempt to repair the camera yourself.
Page 5 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 AC adapter When unplugging the power supply cord, do not pull on the cord. Remove the plug from the outlet to avoid causing electric shock or fire. When unplugging the AC adapter, do not pull on the cord. Remove the plug from the camera to avoid breakdown of the AC adapter or the camera.
Page 6: Check The Contents Of Package
2. CHECK THE CONTENTS OF PACKAGE When opening the package, check that the following items are included before use. If the contents are incorrect, insufficient, or damaged in any way, contact a Hamamatsu subsidiary or your local distributor before attempting to operate the camera.
Page 7: Installation
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 3. INSTALLATION Avoid using or storing this camera in the following places Places where the temperature is not the operating temperature indicated in the • specifications. Places where the temperature is not the storage temperature indicated in the •...
Page 8: Table Of Contents
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 Contents 1. SAFETY PRECAUTIONS ................1 SYMBOLS ......................1 CLASSIFICATION OF WARNINGS ..............1 2. CHECK THE CONTENTS OF PACKAGE ........... 4 3. INSTALLATION ................... 5 4. OVERVIEW ....................8 5. FEATURES ....................8 6.
Page 9 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 15-2 REPAIRS ......................44 16. CONTACT INFORMATION ............... 45...
Page 10: Overview
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 4. OVERVIEW C11440-42U40 is equipped with the new scientific image sensor, an advanced CMOS device that realizes the multiple benefits of high resolution, high readout speed, and low noise all at once.
Page 11 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 (5) Frame rate The CMOS image sensor realizes both low noise (0.9 electrons (median) 1.5 electrons (rms)) and high speed readout (30 fps with 2048 pixels x 2048 pixels) simultaneously, by a split readout scheme in which the top and the bottom halves of the sensor are readout independently, and the data of each horizontal line is read by 2 lines of column amplifier and A/D in the top and the bottom in parallel and simultaneously.
Page 12: Name And Function Of Parts
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 6. NAME AND FUNCTION OF PARTS Do not place the rear panel of the camera, which connectors are • located, to be at the bottom (Do not block ventilation openings.). ① Lens mount C11440-42U40 can be attached to C-mount lens or an optics system.
Page 13 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 ⑥ Power switch [POWER] The power is turned ON/OFF. When the power switch is set to "ON", the camera turns on and starts initialization and the lamp blinks in green. When the initialization is completed , the lamp color stays in green.
Page 14: Connection
This is the cable to connect the USB interface connector of the camera and the USB interface connector on the computer. • Hamamatsu recommends A12046-03 optional USB interface cable for this camera. Note The camera complies with EMC and FCC regulations with using A12467-03 USB interface cable.
Page 15: Operations
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 8. OPERATIONS OPERATING PRECAUTIONS Be careful of the following when you operate the camera. (1) Cooling method Cooling of this equipment is done using a Peltier element. With a Peltier element, when current is supplied, one surface is cooled, and the other surface is heated.
Page 16: Imaging
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 IMAGING Start the control and imaging with the application software. Note • Please refer to the instruction manual attached to the software for the way of using it and the details. END OF IMAGING Carry out the procedure below when imaging is finished.
Page 17: Startup Dcam Configurator
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 STARTUP DCAM CONFIGURATOR The following is a procedure to startup “DCAM Configurator”. (1) Open “Setup.exe” in the DCAM-API software’s folder. If the DCAM-API software is not installed on your computer, insert the media of DCAM-API software in the slot of your computer.
Page 18 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 (4) “DCAM Configurator” window is displayed. The startup is completed with this. - Even if the camera's power supply is turned off, the state of setting is kept. - The state of setting can confirm according to “Hardware” icon on DCAM Configurator window.
Page 19: Description Of Various Functions
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9. DESCRIPTION OF VARIOUS FUNCTIONS THEORY OF THE CMOS IMAGE SENSOR The pixel of the CMOS image sensor is composed of the photodiode and the amplifier that converts the charge into voltage. Entered light is converted to charge and converted to voltage in the pixel.
Page 20 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 The exposure and the readout method of the CMOS image sensor is rolling shutter. In the rolling shutter, the exposure and readout are done line by line. Therefore, the exposure timing is different on one screen. (Figure 9-2) But even if the object moves during the exposure, the affect of rolling shutter is very small.
Page 21: Normal Area Mode
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 NORMAL AREA MODE 9-2-1 Readout method (Scan mode) With normal area mode, the camera reads out the CMOS image sensor from the center line to the top and from the center line to the bottom simultaneously (center line is depicted in red line in the diagram).
Page 22: Readout Method (Scan Speed)
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-2 Readout method (Scan speed) The standard scan readout speed can achieve a frame rate of 30 fps for full resolution with low noise (0.9 electrons (median), 1.5 electrons (rms)), and the rapid rolling can achieve a frame rate of 40 fps for full resolution with low noise (1.3 electrons (median), 1.9 electrons (rms)).
Page 23: Camera Operation Modes
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-3 Camera operation modes The camera has the following operation modes. (1) Free running mode The camera has the free running mode which the exposure and readout timing can be set by software command and controlled by an internal microprocessor. The free...
Page 24: Frame Rate Calculation
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-4 Frame rate calculation (1) Standard scan = Number of vertical line (at the center area of the image sensor) Exp1 = 3.021 ms to 10 s (input in units of seconds) = 32.4812 ×...
Page 25 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 (2) Rapid rolling = Number of horizontal pixel = Number of vertical line (at the center area of the image sensor) Exp1 = 1 ms to 10 s (input in units of seconds) Exp2 = 1.05 ms to 10 s (input in units of seconds)
Page 26: Configuring Exposure Time
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-5 Configuring exposure time The exposure time setting can be done by absolute value. The actual exposure time setting is defined by the following formula, and the camera automatically calculates a larger and closest value from the specified exposure time setting.
Page 27: Timing Chart Of Camera Operation Modes
(in which the exposure time is shorter than the 1 frame readout time). These readout modes are automatically switched depending on the exposure time setting. • Please contact to Hamamatsu subsidiary or local distributor for the detail of the timing Note information.
Page 28 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-1-2 Electrical shutter mode The electrical shutter mode is used to get a proper signal level when signal overflow happens due to too much input photons in normal readout mode. In this mode, the fastest frame rate is 30 fps in Standard scan and 40 fps in Rapid rolling at full resolution even when the exposure time is short.
Page 29 The camera has various external trigger functions to synchronize the camera with the external equipment. In the external trigger mode, the external equipment becomes a master and the camera becomes a slave. • Please contact to Hamamatsu subsidiary or local distributor for the detail of the timing Note information. 9-2-6-2-1 Edge trigger mode The edge trigger mode is used so that the exposure starts according to an external signal.
Page 30 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-2-2 Level trigger mode The level trigger mode is used to control both exposure start timing and exposure time length by inputting external trigger pulses. In this mode, the camera starts exposure at the start of high or low period of the input trigger pulse and stops exposure at the end of high or low period of the input trigger pulse.
Page 31 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-2-3 Synchronous readout trigger mode The synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame from an external source. It is useful for confocal microscopy.
Page 32 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 (2) Trigger times Also in the synchronous readout trigger mode, synchronous readout can be controlled by specifying, set by command, the number of timing pulses to determine the exposure time. The following figure shows the exposure timing when the Trigger Times is set as...
Page 33 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-2-4 Start trigger mode The start trigger mode is to start operating the camera by a trigger input for a continuous imaging. It is useful to secure the frame rate as fast as possible when continuous image acquisition and not to sacrifice the exposure time.
Page 34 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-3 Trigger output The camera provides a range of trigger output signals to synchronize with an external instrument and the camera becomes the master and the external instrument becomes the slave. There are three different trigger output functions as follows.
Page 35 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-3-2 Programmable timing output By using the programmable timing output, synchronizing external devices is simple. A system that needs simple timing signal does not require a delay unit or pulse generator. It is possible to program and output a pulse that has an optional pulse width and an optional delay time to the end of readout timing or Vsync.
Page 36 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-4 Global reset Global reset function enables to reset the electric charge of all pixels at the same time. Then all pixels can start exposure at the same time. Global reset can work with Edge trigger mode and Level trigger mode.
Page 37: Real-Time Correction Functions
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 9-2-6-4-2 Level trigger mode Figure 9-13 REAL-TIME CORRECTION FUNCTIONS There are a few pixels in the CMOS image sensor that have slightly higher readout noise performance compared to surrounding pixels. The camera has real-time variant pixel correction features to improve image quality.
Page 38: Precaution When Using The Cmos Image Sensor
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 10. PRECAUTION WHEN USING THE CMOS IMAGE SENSOR This camera uses the scientific CMOS image sensor. Careful attention must be paid to the following points when using the CMOS image sensor: (1) White spot CMOS image sensor has some high dark current pixels caused by the defect of silicon wafer.
Page 39: Maintenance
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 11. MAINTENANCE 11-1 CARE Perform cleaning of the camera with the dry soft cloth. • Do not wipe with a damp cloth or unclean cloth. Then, the glass window on the CMOS image sensor should be cleaned according to the following.
Page 40: Troubleshooting
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 12. TROUBLESHOOTING If an anomaly occurs, look up the possible causes in the following tables and, if necessary, report the details to a Hamamatsu subsidiary or your local distributor. 12-1 IMAGE IS NOT TRANSFERRED Cause...
Page 41: Specifications
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 13. SPECIFICATIONS 13-1 CAMERA SPECIFICATIONS (1) Electric specifications Imaging device Scientific CMOS image sensor Effective number of pixels 2048 (H) × 2048 (V) Cell size 6.5 µm (H) × 6.5 µm (V) Effective area 13.312 mm (H) ×...
Page 42 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 3 ms to 10 s (Standard scan) Free running mode 1 ms to 10 s (Rapid rolling) 129.99 µs to 10 s (Standard scan) Free running mode Exposure time / Sub-array mode 40 µs to 10 s (Rapid rolling)
Page 43 ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 (3) Operating environment 0 ºC to + 40 ºC (Fan Speed: Fast) Ambient operating temperature 0 ºC to + 35 ºC (Fan Speed: Middle, Slow) Ambient storage temperature -10 ºC to + 50 ºC...
Page 44: Spectral Response Characteristics(Typ.)
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 13-2 SPECTRAL RESPONSE CHARACTERISTICS(TYP.) Figure 13-1...
Page 45: Dimensional Outlines
ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40 Instruction manual_Ver.1.1 14. DIMENSIONAL OUTLINES 14-1 C11440-42U40...
Page 46: Warranty
You must first clarify the symptoms in order to avoid any misunderstanding or error. (2) If you have any trouble or are unclear about anything, contact a Hamamatsu subsidiary or your local distributor giving the product name, serial number and details of the problem. If Hamamatsu Photonics consider the problem to be a malfunction, we will decide whether dispatch an engineer or have the camera returned to us for repairs.
Page 47  The contents of this manual are subject to change without notice.  The unauthorized reproduction or distribution of parts or all of this manual is prohibited.  If one of the following problems occurs, please contact Hamamatsu Photonics. (See the CONTACT INFORMATION.) We will deal with the problem immediately.

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