Olympus IX2-DICD Instructions Manual

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INSTRUCTIONS

IX2-DICD

DIC CONDENSER

This instruction manual is for the Olympus DIC Condenser Model IX2-DICD. To ensure the safety, obtain

optimum performance and to familiarize yourself fully with the use of this equipment, we recommend

that you study this manual thoroughly before operating the system. Retain this instruction manual in an

easily accessible place near the work desk for future reference.

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Table of Contents

Summary of Contents for Olympus IX2-DICD

Page 1 IX2-DICD DIC CONDENSER This instruction manual is for the Olympus DIC Condenser Model IX2-DICD. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this equipment, we recommend that you study this manual thoroughly before operating the system. Retain this instruction manual in an easily accessible place near the work desk for future reference.
Page 3: Table Of Contents
IX2-DICD CONTENTS IMPORTANT — Be sure to read this section for safe use of the equipment. — 1 NOMENCLATURE 2 ASSEMBLY 5-13 3 OBSERVATION METHODS 14-17 3-1 Brightfield Observation ............................................14 3-2 DIC Observation ..............................................14-17 Cross-Nicol Adjustment Observation Procedure...
Page 4: Important - Be Sure To Read This Section For Safe Use Of The Equipment
IMPORTANT · This product is a slim, single type condenser that can be combined with a top lens matching the desired observation (dry, oil-immersed or water-immersed). · The water-immersed top lens (IX2-TLW) features an NA of 0.9, WD or 3.7 mm and manipulator access angle of 40°. In high- resolution DIC (Differential Interference Contrast) observation, these features allow injection operation to be performed simultaneously.
Page 5 IX2-DICD Caution If the condenser is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the system as outlined in this instruction manual.
Page 6: Nomenclature
NOMENCLATURE DIC Condenser IX2-DICD Polarizer clamping knob Condenser height fine adjustment knob Polarizer rotation dial Polarizer engagement knob Aperture iris diaphragm lever DIC prism slider Slider clamping knob (x 2 provided) DIC prism slider insertion slot Knob Top lens mount...
Page 7 IX2-DICD Water-Immersed Top Lens Dry Top Lens Oil-Immersed Top Lens U-TLD U-TLO IX2-TLW DIC Slider DIC Prism U-DICT U-DICTS U-DICTHC U-DICTHR Prism position selector lever * Pushed in: BFP1 Pulled out: NORMAL See the applicable objective Prism movement knob list (pages 7 to 12).
Page 8: Assembly
ASSEMBLY 2-1 Attaching the Top Lens }According to the desired observation, attach the dry (U-TLD), oil-immersed (U-TLO) or water-immersed (IX2-TLW) top lens to the DIC condenser. 1. The U-TLD or U-TLO top lens @ can be attached by screwing it clockwise ²...
Page 9 IX2-DICD 2-2 Attaching the DIC Prism }In the DIC prism slider, mount the DIC prism matching the objective, DIC slider and top lens in use. (For the combination list, see pages 7 to 12.) ² Procedure (Figs. 2 & 3) # To ensure the optical performance, do not tighten the clamping screws @ and | too much.
Page 10 UIS2 Series Combinations of Objectives, DIC Sliders, Top Lenses and DIC Prisms To be used in the BFP1 position of the DIC slider. DIC Slider U-DICT U-DICTS (Shift type) Top Lens U-TLD/IX2-TLW U-TLO U-TLD/IX2-TLW U-TLO UPlanFLN 10X2 U-DIC10 U-DIC10 U-DIC20 U-ODIC20 U-DIC20 U-ODIC20...
Page 11 IX2-DICD U-DICTHC DIC Slider U-DICTHR (High Resolution type) (High Contrast type) Top Lens U-TLD/IX2-TLW U-TLD/IX2-TLW U-TLO UPlanFLN 10X2 U-DIC10HC U-DIC10HR U-DIC20HC U-DIC20HR U-DIC40HC U-DIC40HR 40XO U-DIC40HC U-DIC40HR U-DIC60HC U-DIC60HR U-ODIC60HR 60XOI 100XO2 U-DIC100HC U-DIC100HR U-ODIC100HR 100XOI2 UPlanSApo U-DIC10HC U-DIC10HR U-DIC20HC...
Page 12 UIS Series Usable regardless of the model number (3, 2 or none) Combinations of Objectives, DIC Sliders, Top Lenses and DIC Prisms To be used in the BFP1 position of the DIC slider. DIC Slider U-DICT U-DICTS (Shift type) Top Lens U-TLD/IX2-TLW U-TLO U-TLD/IX2-TLW...
Page 13 IX2-DICD DIC Slider U-DICT U-DICTS (Shift type) Top Lens U-TLD/IX2-TLW U-TLO U-TLD/IX2-TLW U-TLO UPlanApo 10XW3 * U-DP10 U-DP10 60XW3 * U-DPO60S U-ODPO60S U-DPO60S U-ODPO60S 60XW3/IR * 20X3/340 * UApo 20XW3/340 * 40X3/340 * U-DPA40 U-DPA40 40XW3/340* U-DPO40S U-ODPO40S 40XOI3/340*...
Page 14 U-DICTHC DIC Slider U-DICTHR (High Resolution type) (High Contrast type) Top Lens U-TLD/IX2-TLW U-TLD/IX2-TLW U-TLO UPlanFl U-DP10HR U-DP20HC U-DP20HR U-DP40HC U-DP40HR 60XOI3 * U-DPO60HC U-DPO60HR U-ODPO60HR 100XO3 * U-DP100HC U-DP100HR U-ODP100HR 100XOI3 * U-DP10HR UPlanApo 10XO U-DPA20HR 20XO3 * U-DPA40HR 40XOI3 * 100XOI3 * U-DP100HC...
Page 15 IX2-DICD U-DICTHC DIC Slider U-DICTHR (High Resolution type) (High Contrast type) Top Lens U-TLD/IX2-TLW U-TLD/IX2-TLW U-TLO UApo 20X3/340 * 20XW3/340 * 40X3/340 * U-DPA40HR 40XW3/340 * 20XOI3/340 * 2-3 Attaching the DIC Condenser }Attach the DIC condenser by referring to the instruction manual for the IX2 series (IX81, IX71, IX51) or IX series (IX70, IX50)
Page 16 2-4 Centering the DIC Condenser }Disengage the analyzer, polarizer, DIC slider and DIC prism from the light ² path to obtain the brightfield observation light path. 1. Turn the condenser height fine adjustment knob @ counterclockwise to loosen it, then push it fully to the right to raise the condenser to the higher ƒ...
Page 17: Observation Methods
}If a plastic petri dish is used, the DIC effect cannot be obtained due to the polarization characteristic of the plastic material. Be sure to use a glass petri dish. }The DIC observation requires the DIC prism and analyzer in addition to the IX2-DICD DIC condenser.
Page 18: Cross-Nicol Adjustment
Cross-Nicol Adjustment (Figs. 5 and 7) 1. Engage the IX2-AN analyzer and DIC slider in the light path. (Refer to the ² … instruction manual for the IX2 series or IX series microscope frame.) ƒ 2. Remove the DIC prism slider @. 3.
Page 19 IX2-DICD 7. Rotate the prism movement knob of the DIC slider in the clockwise direction all the way until the knob is stopped. A black interference stripe then a rain- bow-colored interference stripe will be observed. Here, stop the knob at the position with which the black interference stripe can be seen.
Page 20: Observation Procedure
Observation Procedure 1. Mount the DIC prism matching the objective to be used in the DIC prism slider, then insert this into the light path. 2. Engage the objective to be used in the light path. }Only when an objective for which the use of the BFP1 position is specified (see pages 7 to 11) is used, push in the prism position selector lever of the DIC slider to the BFP1 position.
Page 21: Troubleshooting Guide
IX2-DICD TROUBLESHOOTING GUIDE Under certain conditions, performance of the equipment may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. Problem Cause Remedy Page The aperture iris diaphragm is Open the aperture iris diaphragm.
Page 22: Specifications
SPECIFICATIONS Specifications Item Dry Top Lens Oil-Immersed Top Lens Water-Immersed Top Lens (U-TLD) (U-TLO) (IX2-TLW) Applicable microscopes IX2 series (IX81, IX71, IX51) or IX series (IX70, IX50) Applicable microscopy Transmitted light (brightfield observation, DIC observation) Type Achromat Aplanat (Top lens replacement system) Number of aperture (NA) 1.4 (oil immersed) Applicable slide glass thickness...
Page 24 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Wendenstraße 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. One Corporate Drive, Orangeburg, NY 10962, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 31 Gilby Road, Mount Waverley, VIC., 3149, Australia 5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.