cytiva EPS 3501 XL Operating Instructions Manual
  1. Manuals
  2. Brands
  3. cytiva Manuals
  4. Power Supply
  5. EPS 3501 XL
  6. Operating instructions manual
cytiva EPS 3501 XL Operating Instructions Manual

cytiva EPS 3501 XL Operating Instructions Manual

Electrophoresis power supply
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Electrophoresis Power Supply
EPS 3501 XL
Operating Instructions
Original instructions
Translation disc included
cytiva.com

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the EPS 3501 XL and is the answer not in the manual?

Questions and answers

Related Manuals for cytiva EPS 3501 XL

Summary of Contents for cytiva EPS 3501 XL

  • Page 1 Electrophoresis Power Supply EPS 3501 XL Operating Instructions Original instructions Translation disc included cytiva.com...
  • Page 2 Page intentionally left blank...

  • Page 3: Table Of Contents

    Eurasian Economic Union (Евразийский экономический союз) .......... 67 8.4.5 North America ............................... 69 8.4.6 Regulatory statements ..........................70 8.4.7 Declaration of Hazardous Substances (DoHS) ................71 8.4.8 Other regulations and standards ......................73 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 4 Table of Contents Health and Safety Declaration Form ...................... 74 Index ........................... 76 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 5: Introduction

    This chapter contains important user information, and intended use of Electrophoresis Power Supply EPS 3501 XL. In this chapter Section See page Important user information About this manual Associated documentation Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 6: Important User Information

    • Agarose electrophoresis • Electroblotting • DNA pulsed field electrophoresis EPS 3501 XL is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes. Prerequisites In order to operate EPS 3501 XL in a safe way and according to the intended purpose, the following prerequisites must be fulfilled: •...
  • Page 7 • You must understand the concepts of electrophoresis. • The product should be installed according to the instructions in Chapter 4 Installa- tion, on page 27 of these Operating Instructions. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 8: About This Manual

    This manual provides you with the information needed to install, operate, and maintain the product in a safe way. Scope of this manual This manual is valid for Electrophoresis Power Supply EPS 3501 XL. The image below shows the product. Notes and tips...

  • Page 9: Associated Documentation

    Component documentation Documentation for components produced both by Cytiva and by a third-party are, if existent, also included in the product documentation. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 10: Safety Instructions

    All users must read and understand the entire contents of this general safety chapter, and the specific safety precau- tions information in each subsequent chapter of this manual to become aware of the hazards involved. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 11: Safety Precautions

    2.1 Safety precautions Safety precautions Introduction EPS 3501 XL is powered by mains voltage and handles materials that may be hazardous. Before installing, operating or maintaining the product, you must be aware of the hazards described in this manual. Definitions This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product.
  • Page 12 Access to power switch and power cord. The power switch must always be easy to access. The power cord must always be easy to disconnect. CAUTION The product is designed for indoor use only. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 13 Installing and moving WARNING Do not block the ventilation inlets or outlets on the system. CAUTION Do not use the product in a dusty atmosphere or close to spraying water. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 14 The high voltage power supply must always be disconnected when the safety lid of the electrophoresis unit is taken off. The high voltage power supply must never be switched on unless the safety lid is on the electrophoresis unit. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 15 WARNING Do not operate the instrument in extreme humidity (above 95%). Avoid condensation by letting the unit equilibrate to ambient temperature when taking EPS 3501 XL from a colder to a warmer environment. WARNING By disabling the start current check, the power supply can deliver high voltage even if it is not connected to electrophoresis equip- ment.

  • Page 16: Labels

    This section describes the system label and other safety labels that are attached to the product. System label The system label is located on the back of EPS 3501 XL. The system label identifies the product and shows electrical data, regulatory compliance, and warning symbols. Label text...
  • Page 17 2 Safety instructions 2.2 Labels Symbol/text Description Warning! High Voltage. Always make sure that the system is disconnected from electric power before opening the cabinet doors or disconnecting any electric device. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 18: Emergency Procedures

    Restart after emergency shutdown or power failure Power failure to EPS 3501 XL will cause the run to stop immediately in an undefined state. Turn on the mains power if EPS 3501 XL is switched off. The program continues when power is resumed.

  • Page 19: Built-In Safety Features

    Functional earth If the power supply is connected to an electrophoresis leakage unit that has a leakage path to earth, EPS 3501 XL will detect this fault and turn off the high voltage. Start current check EPS 3501 XL checks that the resistance is not higher than a specified limit at a low safety voltage (<40V).

  • Page 20: System Description

    3 System description System description About this chapter This chapter gives an overview of EPS 3501 XL, and a brief description of its function. In this chapter Section See page Instrument Front panel Rear panel Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 21: Instrument

    Two electrophoresis units can be connected to EPS 3501 XL and run with the same programmed method at one time. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 22: Front Panel

    Output sockets for two electrophoresis units HV on turns green, when the high voltage power supply is on Keyboard with 9 membrane keys Alphanumeric display Display Part Function Mode Voltage Current Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 23 The keys can also be used to switch between time and volt hours in RUN, PAUSE and END mode. The values scroll, i.e., they automatically change from maximum to minimum value or vice versa. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 24 COPY: To copy a program. CLEAR: To clear a program SETUP: To disable the start current check. MORE mode cannot be activated in RUN or PAUSE mode. Leave the MORE mode by pressing exit. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 25 The voltage output is 0 to 3500 V. Output socket Label Color Voltage Positive 0 to 1700 V Negative Black or blue 0 to -1700 V Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 26: Rear Panel

    Press O to switch off the power Power inlet Voltage range switch The left position corresponds to 100 to 120 V and the right to 220 to 240 V Fan vents Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 27: Installation

    Do not block the ventilation inlets or outlets on the system. WARNING Access to power switch and power cord. The power switch must always be easy to access. The power cord must always be easy to disconnect. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 28 WARNING Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of this product. CAUTION Do not use the product in a dusty atmosphere or close to spraying water. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 29: Transport And Unpacking

    4.1 Transport and unpacking Transport and unpacking Unpacking EPS 3501 XL is delivered in protective packing material and must be unpacked with care. • Check the contents against the packing list supplied. • Record on the receiving documents if there is any apparent damage on the delivery box.
  • Page 30 Place the instrument on a stable and level laboratory bench. WARNING Access to power switch and power cord. The power switch must always be easy to access. The power cord must always be easy to disconnect. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 31: Electrical Connections

    Action Select the appropriate mains cable and connect one end to the power inlet on the rear panel of EPS 3501 XL power supply, see Illustration of the rear panel, on page 26. Connect the other end to a grounded outlet.

  • Page 32: Operation

    Extreme caution must be exercised in its operation. WARNING Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of this product. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 33 Do not operate the instrument in extreme humidity (above 95%). Avoid condensation by letting the unit equilibrate to ambient temperature when taking EPS 3501 XL from a colder to a warmer environment. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 34: Starting The Instrument

    Voltage is same regard- less of the number of units. The current should also be doubled if two gels are run on the same unit. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 35: Setting Up A Run

    This section provides the information required to enter and edit a program. In this section Section See page 5.2.1 Entering a program 5.2.2 Editing a program 5.2.3 Optional programming 5.2.4 Choosing run parameters Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 36: Entering A Program

    10 W 1000 V 10 mA 10 W Follow the steps below to choose a program. Note: The examples in the steps below are as depicted in the flow chart above. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 37 Choosing step means that voltage, current and power limiting values are programmed. The electrophoresis will be controlled by one of these limiting values, which means that it is run at either constant voltage, current or power. EPS 3501 XL automatically switches over the controlling parameter according to programmed limits and conduc- tivity variations in the system.
  • Page 38 (for the next phases) as starting point and the programmed endpoint as endpoint. The electrophoresis is controlled by this voltage gradient provided the limiting current or power is not attained. EPS 3501 XL thus automatically switches over the controlling parameter according to the programmed limits and conductivity varia- tions in the system.
  • Page 39 For automatic break, choose breakpoint unit in either hours (h), volthours (Vh) or milli- amperehours (mAh). Select the correct unit or, for manual break, choose “OFF” with ↑/↓ . Confirm with set enter. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 40 If a phase containing invalid values (zeros) is left, this phase will be deleted. Disabling the start current check See Section 5.2.3 Optional programming, on page 43 if you want to use this feature. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 41: Editing A Program

    It is not possible to insert or delete a phase for a running program. Editing a running program See Section 5.3 Performing a run, on page 48. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 42 5 Operation 5.2 Setting up a run 5.2.2 Editing a program Copying and clearing a program See Section 5.2.3 Optional programming, on page 43. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 43: Optional Programming

    Select to which program number the program should be copied. Empty (available) programs are shown in the lower row. Select with ↑/↓ and confirm with set enter. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 44 Note that a message showing whether the start current check is ON or OFF is shown for a few seconds every time run is pressed. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 45: Choosing Run Parameters

    Choosing run parameters Background information EPS 3501 XL is an automatic cross-over power supply that allows the user to set limits for voltage, current and power. It is also possible to program linear voltage gradients. During electrophoresis, only one of the parameters is limiting at a time. The limiting parameter determines, together with the conductivity in the electrophoresis system, the values for the other two parameters.
  • Page 46 (phase III) to prevent local overheating. For detailed information about parameter values, always follow the gel supplier’s recommendations. The figure below shows the changes in power, voltage and current during an IEF run. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 47 5 Operation 5.2 Setting up a run 5.2.4 Choosing run parameters Power Preset time Alarm Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 48: Performing A Run

    A light emitting diode shows when voltage is applied (HV on). If no current is displayed or if “HALT →Low start current” is shown, check the elec- trical connections to the electrophoresis equipment. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 49 Switch between time, integrated voltage and integrated current for the phase running when pause continue was pressed with ↑/↓ . When sample loading is complete, press either pause continue again or run to continue the run from where it was interrupted. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 50 During a run you can view the settings. It is also possible to interupt the run and make changes in the program. Stop the run manually. The run is stopped automatically. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 51 STOP. Disconnect the leads and proceed with post-electrophoretic techni- ques. Since diffusion will begin as soon as the voltage is turned off, you should remove the gel and begin staining, blotting or autoradiography immediately. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 52: Quick Guide

    After all phases have been programmed, you will be asked if you wish for alarms to sound after the different phases. Press set enter for no (N) or change to yes (Y) and then press set enter. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 53 5 Operation 5.4 Quick guide Step Action When programming is completed, connect your electrophoresis unit to the outputs, and press run. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 54: Maintenance

    WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 55 If wetted, unplug the power supply until the instrument is dry. Servicing All servicing should be entrusted to qualified personnel only. Please contact your local Cytiva representative for more service information. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 56: Troubleshooting

    7 Troubleshooting Troubleshooting About this chapter This chapter contains information of troubleshooting when using EPS 3501 XL. Troubleshooting If an error occurs, either during a run or when switching on the power supply, the program enters the HALT mode and the output is switched off. Four different types of errors can cause HALT.
  • Page 57 7 Troubleshooting FAIL: Code No: xxx Call service Please note the error code number and contact your local Cytiva representative. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 58: Reference Information

    About this chapter This chapter contains reference information for EPS 3501 XL. In this chapter Section See page Specifications Ordering information Recycling information Regulatory information Health and Safety Declaration Form Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 59: Specifications

    8 Reference information 8.1 Specifications Specifications Technical specifications The table below lists the technical specifications for EPS 3501 XL Specification Description Programming 9 programs each with up to 9 phases Regulation Maximum voltage, power and current with automatic crossover at preset limits Line regulation <...
  • Page 60 < 70 dB A Dimensions (WxDxH) 250 × 315 × 95 mm Weight 4.7 kg Transient level Overvoltage category II Pollution degree Electrical specifications The table below lists the electrical specifications for EPS 3501 XL. Specifica- Voltage Current Power Time Volthour Amperehour tion...

  • Page 61: Ordering Information

    8 Reference information 8.2 Ordering information Ordering information Product Code No Electrophoresis Power Supply EPS 3501 XL 18113005 For further information, visit cytiva.com. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 62: Recycling Information

    Waste electrical and electronic equipment must not be disposed of as unsorted munic- ipal waste and must be collected separately. Contact an authorized representative of the manufacturer for information concerning the decommissioning of the equipment. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 63: Regulatory Information

    8 Reference information 8.4 Regulatory information Regulatory information Introduction This section lists the regulations and standards that apply to EPS 3501 XL. In this section Section See page 8.4.1 Contact information 8.4.2 European Union and European Economic Area 8.4.3 Great Britain 8.4.4...

  • Page 64: Contact Information

    The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 65: European Union And European Economic Area

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 66: Great Britain

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 67: Eurasian Economic Union (Евразийский Экономический Союз)

    Telephone: +7 985 192 75 37 E-mail: rucis@cytiva.com Информация о производителе и импортере В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 68 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 69: North America

    Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equip- ment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.

  • Page 70: Regulatory Statements

    When used in a residential environment, there is a concern of radio interference. 유의사항 A급 기기 (업무용 방송통신 기자재) 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받 은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습 니다. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 71: Declaration Of Hazardous Substances (Dohs)

    EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 72 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...

  • Page 73: Other Regulations And Standards

    This section describes the additional standards that apply to the product. Regulatory compliance of connected equipment Any electrical equipment connected to EPS 3501 XL should meet the safety require- ments of EN/IEC 61010-1, or relevant standards. Within EU, connected equipment must be CE marked.
  • Page 74 Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 75 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.
  • Page 76 Flammable liquids, 13 precautions, 13 On site service, 74 Front panel, 22 Operation, 14 display, 22 precautions, 14 Ordering information, 61 Output sockets, 25 General precautions, 11 Gradient programming, 37 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 77 Run, 48, 49, 51 pause, 49 start, 48 stop, 51 Run parameters, 45 Safety features, 19 Safety labels, 16 Safety notices, 11 Safety precautions, 11 introduction, 11 Self diagnostic test, 31 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 29115054 AC...
  • Page 78 Page intentionally left blank...
  • Page 79 Page intentionally left blank...
  • Page 80 Cytiva and the Drop logo are trademarks of Life Sciences IP Holdings Corp or an affiliate doing business as Cytiva. ExcelGel and Immobiline are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.

Table of Contents