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Related Manuals for Benchmark Scientific MyGel E1201-E
Summary of Contents for Benchmark Scientific MyGel E1201-E
- Page 1 MyGel™ InstaView Electrophoresis System Operating Manual Version 1.0...
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Page 2: Table Of Contents
Keep this manual for future reference. Please check the list of included components in section 1.1, and if there are any parts missing, damaged, or incorrect, please contact your distributor or Benchmark Scientific’s Customer Service Department at 908-769-5555 or by email at info@benchmarkscientific.com. -
Page 3: Included Component List
The MyGel InstaView is a complete horizontal electrophoresis system that includes a power supply, gel running tank, safety lid with viewing filter, blue LED transilluminator, and a gel casting set. The system is designed for separating nucleic acids in agarose gels, and allows viewing the stained DNA bands during electrophoresis without removing the gel from the tank. -
Page 4: Diagrams Of Included Components
1.2 Diagrams of Included Components Smart phone imaging enclosure with photo filter Safety Lid with Integral Viewing Filter Gel Running Tank Power Supply AC Power Cord 12V Power Adapter Blue LED Transilluinator... - Page 5 Diagram of Included Components (Gel Casting Set Double Sided Comb (12/22 teeth) Short Gel Trays (10.5 x 6cm) Long Gel Tray (10.5 x 10cm) Gel Casting Divider Gel Casting Stand...
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Page 6: Specifications
1.3 Product Specifications Power Supply 115V 50/60Hz or 230V 50/60Hz (Transformer Input voltage included with 230V input model) Output voltage 35V, 50V, 100V Max power 40 Watt 0 ～ 99min Timer Fuse 250V, 2A Migration Tank Dimension 120mm×110mm×45mm（inner dimension） Buffer volume 200ml-225ml Electrodes Platinum wire... -
Page 7: Installation
Do not attempt to open or repair the MyGel system. Contact your distributor or Benchmark Scientific for service. Always use the MyGel in an environment with low humidity and low dust, also keep away from water, direct sunlight / strong light, corrosive gas, high magnetic fields, heaters, fires and other heat sources. -
Page 8: Gel Preparation
4. Gel Preparation Place the gel casting stand on a level surface, and place the divider and desired gel trays into the proper positions in the stand. Either one long gel tray, or two short trays can be inserted into the casting stand (see diagrams on right) Note: If the gel tray is not level, the thickness of the gel will not be uniform and DNA... - Page 9 Pour the agaose gel/DNA stain solution into the gel tray to make a gel approximately 4mm thick. Each 6x10.5cm tray requires about 25ml of agarose. Note: After mixing and heating agarose solution, let it cool to approximately 60°C before pouring into the gel trays After the gel has solidified (approximately 20 minutes) hold the two sides of comb and gently lift it out...
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Page 10: Gel Tank Set Up
5. Gel Tank Set Up Note: Make sure that the MyGel System is installed on a stable and level surface. This will ensure even sample migration. Place a prepared gel, together with the gel tray onto the gel bed inside the gel tank. Carefully pour an appropriate buffer solution to a level about 2 mm above the surface of the gel. - Page 11 Add DNA samples to the gel Use an appropriate pipette to carefully dispense samples into the wells of the gel Note: Loading buffer should be mixed with the DNA samples so they will sink to the bottom of the wells. Carefully lower the safety cover onto the gel tank, making sure to align the magnet with the sensor recess.
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Page 12: Power Supply Operation / Electrophoresis
6. Power Supply Operation / Electrophoresis Connect the Connect the power cord to the 12V power power supply and supply to the plug into the appropriate wall transilluminator outlet. Press to set the run time from 1 to 99 min. For a continuous run, set the timer to “00”. -
Page 13: Viewing Electrophoresis Progress
7. Viewing Electrophoresis Progress To visualize the DNA separation in the gel during electrophoresis, turn on the blue LED illuminator using the ON/OFF switch. It may be necessary to dim or turn off ambient lights for best viewing results. The LED illuminator has a built in timer, so the light will automatically switch off after 5 minutes. -
Page 14: Maintenance
9. Maintenance Always disconnect the power cords for cleaning. When cleaning the surfaces of the instrument and LED transilluminator, use a damp cloth with mild detergent if needed. Do not use any corrosive solutions that could damage plastic. When cleaning the gel tank, first separate the Power Supply. - Page 15 Troubleshooting (continued Problem Causes Resolutions Lid opened during run or Seat lid properly. Check Error E1 magnet switch problem. magnet on lid.. Seat lid properly. Check Lid not closed upon start or magnet on lid. Error E3 magnetic switch problem. Check proper buffer level in the tank.
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