cytiva Amersham TYPHOON Operating Instructions Manual
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cytiva Amersham TYPHOON Operating Instructions Manual

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Operating Instructions
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  • Page 1 Amersham TYPHOON Operating Instructions Original instructions cytiva.com...

  • Page 2: Table Of Contents

    Installation ......................65 Site requirements ............................66 Delivery and transport ..........................69 Setup ................................... 70 Connections ..............................72 Operation ......................74 Preparing the Amersham TYPHOON scanner ................... 76 6.1.1 Starting the system ............................. 77 6.1.2 Changing the filters ............................. 79 6.1.3 Custom filters ..............................
  • Page 3 North America ............................... 123 9.4.6 Regulatory statements ..........................124 9.4.7 China .................................. 125 9.4.8 Other regulations and standards ......................128 Ordering information ........................... 129 Health and Safety Declaration Form ...................... 130 Index ........................... 132 Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 4: Introduction

    About this chapter This chapter contains important user information, abbreviations used within the manual, and intended use of the Amersham™ TYPHOON system. In this chapter Section See page Important user information About this manual Abbreviations Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 5: Important User Information

    Intended use of the Amersham TYPHOON scanner The Amersham TYPHOON scanner is a versatile laser scanner that can be used for many different imaging applications, including: • imaging of storage phosphor screens • fluorescence imaging with multiple lasers, for example for muliplex Western blot- ting and two-dimensional difference gel electrophoresis (2D DIGE) •...

  • Page 6: About This Manual

    Scope of this manual The Operating Instructions cover all configurations of Amersham TYPHOON scanner and the Amersham TYPHOON Control Software. The illustration below shows the Amersham TYPHOON scanner. Descriptions of Amersham ERASER and Amersham Cabinet are not covered in this document.

  • Page 7: Abbreviations

    Red Green Blue Red Green Blue Relative humidity Relative humidity Radioisotope Radioisotope Second harmonic generation Second harmonic generation nonlinear optical process nonlinear optical process Titer plate Titer plate Ultraviolet radiation Ultraviolet radiation Near-infrared radiation Near-infrared radiation Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 8: Safety Instructions

    Safety precautions Labels and symbols Emergency procedures Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 9: Radiation

    However, as described above, the storage phosphor screen surface can be polluted by radioisotope (RI), depending on the sample condition, since the instrument sticks the sample to the H-compatible storage phosphor screen surface and exposes it in an auto-radiography experiment of the H label sample. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 10 Limit of superficial pollution, on page 9 above. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 11: Safety Precautions

    2.2 Safety precautions Safety precautions Introduction Amersham TYPHOON scanner is powered by mains voltage and handles materials that can be hazardous. Before installing, operating or maintaining the system, you must be aware of the hazards described in this manual. Follow the instructions provided to avoid personal injuries, damage to the product, or to other personnel and equipment in the area.
  • Page 12 Do not damage the power supply cord by bending, twisting, heating or allowing them to become pinned under the equipment. Using damaged power cords could result in fire or electric shock. If the power supply cords are damaged, contact your local Cytiva representative for replacements. WARNING Do not allow liquids, flammable materials or metallic objects to get into the product.
  • Page 13 The product must always be connected to a grounded power outlet. WARNING Do not block access to the power switch and power cord. The power switch must always be easy to access. The power cord with plug must always be easy to disconnect. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 14 CAUTION Only authorized service personnel are allowed to install or move the product. Contact your local Cytiva representative for help and advice. CAUTION Install the product in a location where it will not come into contact with water or chemicals.
  • Page 15 • Do not connect any USB devices other than the product to the computer in which the Amersham TYPHOON Control Software is installed. Malfunction could result. • Use only the Amersham TYPHOON Control Software during scanning. CAUTION Connect the computer hardware on the same power circuit as the scanner, otherwise the equipment may be influenced by electrical noise.
  • Page 16 Reagents used to prepare the sample should be used in accord- ance with the manufacturer instructions. CAUTION Do not leave samples in the instrument after scanning. If left, these may degrade and cause damage to the instrument. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 17 Do not attempt to modify the instrument, or fire and electric shock could result. WARNING Do not use excessive amounts of liquids for cleaning the product, this may result in product malfunction or electric shock. CAUTION Wear gloves to prevent direct contact with chemical substances. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 18 Turn off the power switch and remove connecting cables before moving the equipment. CAUTION Turn the power switch off before cleaning the inside of the equip- ment. CAUTION Unplug the equipment if it will not be used for an extended period. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 19: Labels And Symbols

    This section describes the nameplate, labels, and other safety and regulatory informa- tion attached to the product. Nameplate The nameplate provides information about the model, manufacturer, and technical data. An example of Amersham TYPHOON label is reported below. Label text Description Model Instrument model...
  • Page 20 Operation is subject to the following two conditions: CAN ICES-3 (A)/NMB-3 (A) (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undersired operation. CAN ICES-3 (A)/NMB-3(A) Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 21 2.3 Labels and symbols Symbols on the Amersham TYPHOON scanner Label Meaning On/Off switch Power switch l: Power ON O: Power OFF USB port Location of labels and symbols on the Amersham TYPHOON scanner Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 22 2 Safety instructions 2.3 Labels and symbols Interlock parts inside the Amersham TYPHOON scanner Never insert any foreign objects into the interlock parts to avoid potential laser expo- sure and injury. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 23: Emergency Procedures

    2 Safety instructions 2.4 Emergency procedures Emergency procedures About this section This section describes how to do an emergency shutdown of Amersham TYPHOON scanner. The section also describes the results of a power failure. Precautions WARNING Access to power switch and power cord with plug. Do not block access to the power switch and power cord.
  • Page 24 If power to the instrument has been lost, re-start the instrument as described in Start the Amersham TYPHOON scanner on page Re-start the computer and Amersham TYPHOON Control Software as described in Start the Amersham TYPHOON Control Software on page...

  • Page 25: System Description

    3 System description System description About this chapter This chapter gives an overview of Amersham TYPHOON scanner and a brief description of its function. In this chapter Section See page System overview Illustrations Stages Filters Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 26: System Overview

    System overview Introduction The Amersham TYPHOON scanner is a laser scanner that can be used for many different imaging applications. The scanner is part of the complete Amersham TYPHOON system, that consists of the scanner, an eraser and a cabinet.
  • Page 27 Main components in Amersham TYPHOON scanner on page 112 Laser specifications on page 112. Scanning modes The Amersham TYPHOON scanner can be used for scanning with three different scan- ning modes. Mode Used for Fluorescence • Scanning fluorescent samples using different combinations of lasers and filters.
  • Page 28 3 System description 3.1 System overview Instrument control The Amersham TYPHOON scanner is operated using Amersham TYPHOON Control Software. The scanner also has an instrument panel with indicator lamps that indicate the status of the instrument. Refer to Instrument panel on page 31 for more informa- tion about the instrument panel.

  • Page 29: Illustrations

    3 System description 3.2 Illustrations Illustrations Front and right view of the Amersham TYPHOON scanner Part Function Instrument panel Main scanner door Scanner door handle Instrument cover Power cord connector Power switch Filter module Filter door Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 30 3 System description 3.2 Illustrations Rear and left view of the Amersham TYPHOON scanner Part Function Air intake filter and fan Exhaust outlet Exhaust outlet USB connector Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 31 • If flashing: Instrument is starting up or shutting down Blue LED light • The instrument is scanning, or • The filters are being moved inside the scanner (after filter installation). Red LED light Error Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 32 3 System description 3.2 Illustrations Filter module and stage Part Function Filter module Stage Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 33: Stages

    This section describes the different stages and accessories that are supplied with the Amersham TYPHOON scanner. In this section Section See page 3.3.1 Overview of stages 3.3.2 Fluor stage with accessories 3.3.3 Phosphor stage with accessories 3.3.4 Multi stage with accessories Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 34: Overview Of Stages

    3 System description 3.3 Stages 3.3.1 Overview of stages 3.3.1 Overview of stages Stages Three types of stages can be used with the Amersham TYPHOON scanner depending on the type of scanning: • Fluor stage • Phosphor stage • Multi stage Accessories The table below lists the different stages and accessories, and for which applications the stages and accessories are used.

  • Page 35: Fluor Stage With Accessories

    The digitization plate is used together with spacers for densitometry (measure- ment of relative optical density) and digitization of samples. Spacers There are two types of spacers that can be used with the fluor stage: Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 36 The illustation below shows the orientation in which the spacers are used for digitization. The illustration below shows the orientation in which the spacers are used for OD measurements. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 37 For digitization, the spacers should be placed with the digitization label facing up, and the arrows pointing to the scan area. For OD measurements, the spacers should be placed with the OD label facing up. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 38 The membrane weight is used to press fluorescent membranes flat against the fluor stage. The use of a membrane weight improves image quality, since the membrane weight removes air bubbles and reduces vibration of the membrane during scanning. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 39: Phosphor Stage With Accessories

    (PMT). In this way, the Amersham TYPHOON scanner creates a digital image of the radiated areas of the storage phosphor screen.
  • Page 40 3 System description 3.3 Stages 3.3.3 Phosphor stage with accessories Suction rod The suction rod is used to lift up or place the storage phosphor screen on the phosphor stage. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 41: Multi Stage With Accessories

    Height adjustment ridges for positioning the titer plate holder and glass cassettes (3 mm and 5 mm) Movable guide plate Lock screw, holds the movable guide plate on the multi stage Movable guide plate Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 42 The glass plate guides are used instead of the default movable guide plates. The thickness of each glass in the glass cassette should be 5 mm. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 43: Filters

    Custom filters It is also possible to use custom (third party) filters. Amersham TYPHOON scanner is supplied with two accessory custom filter holders in which these custom filters can be mounted. These filters are recognized by the scanner as Custom1 and Custom2 filters.

  • Page 44: Amersham Typhoon Control Software

    4 Amersham TYPHOON Control Software Amersham TYPHOON Control Software About this chapter This chapter describes the user interface of the Amersham TYPHOON Control Software. In this chapter Section See page Main window Scanning modes General settings Scan area functions Image file settings...

  • Page 45: Main Window

    This section gives an overview of the main window and describes the settings that can be made. Example of the main window When the Amersham TYPHOON Control Software is started, the main window appears. The main window contains the following panes and fields. Part...
  • Page 46 4 Amersham TYPHOON Control Software 4.1 Main window Part Function Reference/Description Help button About the Amersham TYPHOON Control Software Scanning mode tabs The scanning mode can be chosen by clicking on one of the scanning mode tabs. Scanning mode Function Fluorescence...
  • Page 47 Adds additional scans. Changes between scan areas (DIGE method). Adds an annotation (DIGE method). An annotation can be deleted with the small cross. Fluorescence mode Phosphor Imaging mode Densitometry mode Only for digitization Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 48 • Blue frame (white area) indicates a scan area that has been disabled. A disabled scan area will be omitted during scanning. For more information about the Stage/Area settings, refer to Section 4.4 Scan area functions, on page Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 49 Method and scanning Button Function Save Method Opens the Input method name dialog box to save the method Delete Method Deletes the method Pre Scan Starts a pre-scan Scan Starts a scan Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 50 List of installed lasers Filter List of installed filters List of photo-multiplier tubes Status Displays the current status of the Amersham TYPHOON scanner. The status of the scanner can be one of the following: • Scanner not connected • Initializing • Ready •...

  • Page 51: Scanning Modes

    The scan settings and Stage/Area area settings can be edited in a default method. An edited method can be saved under a new name for re-use at a later time. The following illustration shows and example of a DIGE method set up screen. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 52 4 Amersham TYPHOON Control Software 4.2 Scanning modes Phosphor imaging tab The following illustration shows an example of a Phosphor Imaging set up screen. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 53 1. OD method for measurements of relative optical density 2. Digitization method blue (blue laser) 3. Digitization method green (green laser) The following illustration shows an example of the OD method set up screen. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 54: General Settings

    Laser and filter combinations Amersham TYPHOON scanner comes with a number of default laser and filter combi- nations. New laser, filter, and detector combinations can be created on the Laser & Filter tab in the General settings dialog.
  • Page 55 For fluorescence scanning, only the multi-alkali PMT can be used. Auto correction Amersham TYPHOON scanner is installed with correction files. It is recommended to always use the correction files (auto correction on). The auto correction can be turned off in the General settings dialog on the Auto correction tab.
  • Page 56 Phosphor Imaging. This selection only affects how images are displayed, it does not affect the saved image. Service log In the General settings dialog on the Service tab, a log file can be generated which is used for instrument service or troubleshooting. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 57: Scan Area Functions

    Delete a scan area on the computer keyboard. Change the position of a scan Click and drag the blue box. area Change the size of a scan area Click and drag the edges of the blue box. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 58 When the name of one scan area is changed, all other scan areas will be renamed as well. Remove... Deletes the scan area. Setting... Opens the dialog box Scan Area Settings, where the size and position of the scan area can be adjusted. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 59 • Upper Right sets the position of the upper right corner of the scan area. Area Assigns a name (A, B, C, ...) to the scan area. When the name of one scan area is changed, all other scan areas will be renamed as well. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 60 Enables or disables an individual scan area. A tick mark indicates that the scan area is enabled and will be included in the scan. Note: A disabled scan area will be omitted during scanning. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 61: Image File Settings

    • For quantitative analysis use the .img format or the .gel file format. • For DIGE applications, and when image properties need to be read in IQTL, use the .gel format. In the Densitometry mode, the data is automatically converted and saved as a .tif file. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 62: View Image Mode

    PMT that gives a non- saturated image. Use the Estimated tab of the Adjust PMT field to simu- Pre-scan late images using different PMT settings. Scanned image. Pre-scan and scan Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 63 Sigmoid The light intensities are displayed using a sigmoid (S-shaped) tone curve. Tip: It is recommended to use the linear curve setting Automatically adjusts the range of light intensities for optimum display. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 64 To display a larger or smaller interval of intensities, drag the red lines to adjust the Low and High values, or type the limits directly in the Low and High boxes. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 65: Installation

    This chapter provides required information to enable users and service personnel to unpack, install, and transport Amersham TYPHOON scanner. CAUTION Only authorized service personnel are allowed to install or move the product. Contact your local Cytiva representative for help and advice. In this chapter Section...

  • Page 66: Site Requirements

    10 cm at left, right, top and rear of the instru- ment Placement Stable, horizontal surface. Do not place in direct sunlight or in brightly lit places. Dimensions The following illustration shows the dimensions of Amersham TYPHOON scanner. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 67 5 Installation 5.1 Site requirements Amersham TYPHOON scanner weighs 90 kg. Environmental requirements Parameter Requirement Ambient temperature, operation 18°C to 28°C Max. relative humidity, operation 20% to 70%, non-condensing Max. altitude, operation 2000 m above sea level Pollution degree of the intended environment...
  • Page 68 The automatic sleep mode of the computer should be turned off to prevent the computer from entering sleep mode during a scan. Otherwise loss of data might occur. Note: At installation, the Cytiva service engineer will turn off the automatic sleep mode of the computer. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 69: Delivery And Transport

    CAUTION Turn off the power switch and remove connecting cables before moving the equipment. If you need to transport the Amersham TYPHOON scanner, contact your local Cytiva representative for help and advice. See also Removal from the controlled area on page...

  • Page 70: Setup

    Amersham TYPHOON scanner. • The air intake fan on the rear side of the Amersham TYPHOON scanner is unob- structed. • The air exhaust outlets on the right and rear of the Amersham TYPHOON scanner are unobstructed.
  • Page 71 NOTICE Do not place the Amersham ERASER and Amersham Cabinet to the right of the scanner, because then they block the access to the power switch and power outlet. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 72: Connections

    Malfunction could result. Step Action Plug in the power cord in the power cord connector on Amersham TYPHOON scanner, and connect the power cord to a grounded wall outlet. Amersham TYPHOON scanner uses a special AC power cord. Only use power cords that are specified in Cytiva service manuals or by Cytiva service personnel.
  • Page 73 • Do not connect any USB devices other than the product to the computer in which the Amersham TYPHOON Control Software is installed. Malfunction could result. • Use only the Amersham TYPHOON Control Software during scanning. NOTICE Any computer used with the equipment must comply with IEC 60950 or IEC 62368-1 and be installed and used according to...

  • Page 74: Operation

    6 Operation Operation About this chapter This chapter contains the information required to operate the Amersham TYPHOON scanner. In this chapter Section See page Preparing the Amersham TYPHOON scanner Preparing the sample Performing a scan Procedures after a scan Precautions...
  • Page 75 NOTICE The automatic sleep mode of the computer should be turned off to prevent the computer from entering sleep mode during a scan. Otherwise loss of data might occur. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 76: Preparing The Amersham Typhoon Scanner

    6 Operation 6.1 Preparing the Amersham TYPHOON scanner Preparing the Amersham TYPHOON scanner About this section This section describes how to prepare the Amersham TYPHOON scanner for a scan. In this section Section See page 6.1.1 Starting the system 6.1.2 Changing the filters 6.1.3...

  • Page 77: Starting The System

    6 Operation 6.1 Preparing the Amersham TYPHOON scanner 6.1.1 Starting the system 6.1.1 Starting the system Start the Amersham TYPHOON scanner CAUTION Do not insert a storage phosphor screen in the product before turning on the machine. If a storage phosphor screen is detected...
  • Page 78 Start the Amersham TYPHOON Control Software Step Action Start Amersham TYPHOON Control Software from the Start menu, or use the desktop shortcut. The following window is displayed while the Amersham TYPHOON Control Software is launched. When the Amersham TYPHOON Control Software is ready, the main window is displayed.

  • Page 79: Changing The Filters

    6.1.2 Changing the filters 6.1.2 Changing the filters Introduction The filters that are installed in the Amersham TYPHOON scanner are listed in the Instrument status pane in the main window of the Amersham TYPHOON Control Software, see also Instrument status on page It is possible to exchange filters if necessary.
  • Page 80 6 Operation 6.1 Preparing the Amersham TYPHOON scanner 6.1.2 Changing the filters Step Action Pinch the metal tab on the filter module, and pull out the filter module. Replace filters in the filter module Step Action Slide the holder on the filter module to the left to open the filter module.
  • Page 81 6 Operation 6.1 Preparing the Amersham TYPHOON scanner 6.1.2 Changing the filters Step Action Pull out the filter from under the metal ridge, and remove the filter. Insert a new filter in the filter module as follows: a. Hook a new filter under the metal ridge on the rear of the filter module.
  • Page 82 Insert the filter module in the filter module holder. A clicking sound indicates that the filter holder is in the correct position. Close the filter door. In the Amersham TYPHOON Control Software, check the list of filters in the Instrument status pane. Make sure that the newly installed filters are correctly registered.

  • Page 83: Custom Filters

    6.1.3 Custom filters Introduction It is possible to use custom (third party) filters with the Amersham TYPHOON scanner. The custom filters have to be installed in the filter holder boxes supplied by Cytiva. Insert a custom filter in the filter holder Follow the steps below to insert a custom filter in the filter holder.

  • Page 84: Preparing The Sample

    Precautions CAUTION Always wear gloves, protective glasses and a lab coat or similar when handling samples. CAUTION Handle the sample stages and the digitization plate with care to avoid dropping them. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 85: Preparing A Sample For Fluorescence Imaging

    Remove any air bubbles between the gel or membrane and the stage. If the sample is a membrane, place a membrane weight on top of the membrane to reduce vibrations during the scan. Open the main scanner door of the Amersham TYPHOON scanner. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 86 Close the spring locks that hold the titer plate holder in place. Place the titer plate on the desired position in the titer plate holder. Open the main scanner door of the Amersham TYPHOON scanner. Place the stage on the stage holder, with the printed side of the stage facing up, and the handle of the stage facing to the front.
  • Page 87 Place the glass slide holder on the titer plate holder, with the glass slides facing down. Open the main scanner door of the Amersham TYPHOON scanner. Place the stage on the stage holder, with the printed side of the stage facing up, and the handle of the stage facing to the front.
  • Page 88 When scanning large glass cassettes (max. 33 × 42 cm) on the multi stage, the default movable guide plates have to be replaced by the glass plate guide for large glass cassettes. These glass plate guides are available as option to the Amersham TYPHOON scanner, see also...

  • Page 89: Preparing A Sample For Phosphor Imaging

    NOTICE Remove the storage phosphor screen from the Amersham TYPHOON scanner when not in use. Store the storage phosphor screens in a horizontal position, protected from mois- ture. NOTICE Do not stack cassettes during exposure.
  • Page 90 Press the suction rod against the storage phosphor screen. Place a finger on the end of the suction rod, then lift up the storage phosphor screen. Release the finger to release the storage phosphor screen. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 91 Dim the ambient light to 20 lux or less before taking out the storage phosphor screen from its package or the cassette. Tip: For increased image quality, minimize the time between exposing the storage phosphor screen and scanning it with the Amersham TYPHOON scanner. Step Action If necessary, clean the surface of the storage phosphor screen with a soft, lint-free tissue.
  • Page 92 Leave the storage phosphor screen inside the cassette for the required exposure time. Note: The exposure time required for a storage phosphor screen is approximately one twentieth of the time required for X-ray film. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 93 Lift up the storage phosphor screen with the suction rod. Place the storage phosphor screen on the bottom of the phosphor stage, with the exposed surface facing up. Release the storage phosphor screen by releasing your finger from the suction rod. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 94 Step Action Open the main scanner door of the Amersham TYPHOON scanner. Turn the phosphor stage around. Place the phosphor stage on the stage holder, with the white print on the phosphor stage facing up, and the storage phosphor screen attached to the bottom of the phosphor stage. The exposed surface of the storage phosphor screen should face down.

  • Page 95: Preparing A Sample For Densitometry

    Prepare the sample The illustrations under the Densitometry tab in the main window of the Amersham TYPHOON Control Software provide instructions for placing gels, spacers and the digitization plate. The instructions provided in the software are adapted to the densitometry method that is chosen.

  • Page 96: Recommended Scan Settings For Densitometry, Nir Scans, And Multi-Well Titer Plates

    6.2.4 Recommended scan settings for densitometry, NIR scans, and multi-well titer plates Amersham TYPHOON allows the user to scan the full stage with any sample. However, for best performance and accurate quantitation, adhere to the following recommenda- tions. Densitometry samples There are two main methods in the Densitometry scan mode: Digitization and OD.
  • Page 97 • the vertical distance from inside of the well bottom to the resting plane of the plate (indicated with arrows in the following figure) must be 2.0 to 3.0 mm. Contact your local Cytiva representative for an updated list of recommended plate suppliers.

  • Page 98: Performing A Scan

    The pre-scan feature is not available in the phosphor imaging mode. Step Action In the main window of the Amersham TYPHOON Control Software, click on one of the scanning mode tabs to choose the scanning mode. See also Scan- ning mode tabs on page...
  • Page 99 • Click Scan to start a scan, • Adjust the PMT voltage, and click Scan to start a scan. Scan the sample NOTICE Avoid any shock or vibration to the instrument during scanning, or some streaks appear in the image. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 100 6 Operation 6.3 Performing a scan Step Action In the main window of the Amersham TYPHOON Control Software, click on one of the scanning mode tabs to choose the scanning mode, see Scanning mode tabs on page Select a saved method from the Method dropdown list in the Scan settings pane, adjust the scan settings to create a new method.
  • Page 101 Select a file format in the Save as type: buttons. For the DIGE method the files are saved in a folder with an additional DS-file. Click Save. Result: The image or images are saved. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 102: Procedures After A Scan

    104. Press the On/Off button on the front panel. Result: The white Ready LED blinks and then goes out. Push the power switch on the right side of the instrument to the O position. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 103 6 Operation 6.4 Procedures after a scan Step Action Result: The instrument is turned off. CAUTION Unplug the equipment if it will not be used for an extended period. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 104: Maintenance

    7 Maintenance Maintenance About this chapter This chapter contains information to enable users to clean, maintain, and store the Amersham TYPHOON scanner and the stages. Precautions WARNING Never detach the inner cover screwed to this instrument. If it is detached, laser beam may leak with a risk of loss of vision.
  • Page 105 • Mild and neutral detergent • Isopropanol • 6% H Storing the stages Store the stages in the cabinet when they are not in use. DO NOT store stages inside the Amersham TYPHOON scanner. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 106: Troubleshooting

    8 Troubleshooting Troubleshooting About this chapter This chapter describes various problems that may occur with the Amersham TYPHOON scanner. Suggestions for possible corrective actions are given. In this chapter Section See page General errors and warnings Error messages Troubleshooting guide...

  • Page 107: General Errors And Warnings

    8 Troubleshooting 8.1 General errors and warnings General errors and warnings If an error occurs When an error message appears in the Amersham TYPHOON Control Software, follow the steps below. Step Action Take note of the error code and error message on the monitor.

  • Page 108: Error Messages

    Close the scanner door and scan scanner door was opened. Close the was opened during the image again. Do not open the door and restart the scan.. the scan. door until the scanning is finished. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 109 Please restart the Amersham Typhoon persists. scanner and control software, and try to scan again. Contact your Cytiva repre- sentative if the problem persists. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 110: Troubleshooting Guide

    Densitometry values vary between gels. The OD values are relative and compari- sons must only be made within the same gel, see Section 6.2.4 Recom- mended scan settings for densitometry, NIR scans, and multi-well titer plates, on page Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 111: Reference Information

    9 Reference information Reference information About this chapter This chapter lists the technical specifications of the Amersham TYPHOON scanner. In this chapter Section See page Specifications Software Recycling information Regulatory information Ordering information Health and Safety Declaration Form Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 112: Specifications

    9 Reference information 9.1 Specifications Specifications Main components in Amersham TYPHOON scanner Amersham TYPHOON scanners are available in four configurations. Depending on the configuration, the Amersham TYPHOON scanner is equipped with the following lasers. Functions Wave- Amersham Amersham Amersham Amersham...
  • Page 113 50/60 Hz Max. current 2.5 to 1.0 A Noise levels Parameter Data Noise < 60 dB (A) A-weighted sound pressure level LAeq, t ≤ 60 dB, fast (t=0.25 s, 1 m from the instrument) Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 114 Lighting It is recommended to lower the lighting level to about 20 lux when moving a sample from the cassette into Amersham TYPHOON after expo- sure. Where to use Indoor use only, out of direct sunlight or brightly lit surroundings.

  • Page 115: Software

    Maximum image file size after scan If pixel size is set as Image file size 10 µm 3510 MB 25 µm 562 MB 50 µm 140 MB 100 µm 35 MB 200 µm 9 MB Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 116: Recycling Information

    Radiation hazard prevention on page If the pollution level exceeds the limit, dispose of the instrument as radioactive waste. Otherwise, dispose of the materials according to applicable laws and regulations for disposal of industrial waste. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 117: Regulatory Information

    See page 9.4.1 Contact information 9.4.2 European Union and European Economic Area 9.4.3 Great Britain 9.4.4 Eurasian Economic Union (Евразийский экономический союз) 9.4.5 North America 9.4.6 Regulatory statements 9.4.7 China 9.4.8 Other regulations and standards Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 118: Contact Information

    The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 119: European Union And European Economic Area

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 120: Great Britain

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 121: Eurasian Economic Union (Евразийский Экономический Союз)

    Telephone: +7 985 192 75 37 E-mail: rucis@cytiva.com Информация о производителе и импортере В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 122 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 123: North America

    Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equip- ment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.

  • Page 124: Regulatory Statements

    When used in a residential environment, there is a concern of radio interference. 유의사항 A급 기기 (업무용 방송통신 기자재) 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받 은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습 니다. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 125: China

    有害物质声明 (DoHS) Declaration of Hazardous Substances (DoHS) 根据 SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下 有关污染控制方面的信息。 The following product pollution control information is provided according to SJ/ T11364-2014 Marking for Restriction of Hazardous Substances caused by electrical and electronic products. 电子信息产品污染控制标志说明 Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 126 Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 127 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 128: Other Regulations And Standards

    This section describes the additional standards that apply to the product. Regulations and standards, products containing lasers Regulation / Standard Description EN/IEC 60825-1 Safety of laser products USA 21 CFR, Chapter I, Safety of laser products Subchapter J, Part 1040.10 Laser Products Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 129: Ordering Information

    Multi stage 29187198 Membrane weight 29191518 Digitization plate 29191517 Sample guide 29191519 Suction rod 29191516 Titer plate holder 29191520 Glass slide holder 29191521 Custom filter boxes 29191540 Glass plate guide (33 × 42 cm) 29215514 Amersham TYPHOON Operating Instructions 29193226 AF...

  • Page 130: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 131 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.

  • Page 132: Index

    73 Amersham Cabinet, 26 Cooling fans, 27 Amersham ERASER, 26 Custom filter, 83 Amersham TYPHOON , 5, 9, 10, 21, install, 83 22, 26, 29–31, 66, 77, 102, 112, 113, configurations, 26, 112, 129 Data communication, 73 description, 26...
  • Page 133 Installation, 70 Personal protection, 13 precautions, 70 Phosphor imaging, 27, 89 Instrument control, 28 precautions, 89 Instrument panel, 29, 31 prepare sample, 89 Instrument status, 45, 50 Phosphor imaging mode, 52 Intended use, 5 Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 134 Safety notices, 11 Stage, 32, 34, 35, 39, 41, 105 Safety precautions, 11–15, 17 accessories, 34 general, 12 fluor, 35 installation, 14 location, 32 introduction, 11 multi, 41 maintenance, 17 overview, 34 operation, 15 phosphor, 39 Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 135 Transport, 69 Troubleshooting, 56, 107 error, 107 service log file, 56 Typographical conventions, 6 UK, 120 conformity, 120 UKCA, 120 marking, 120 USB connector, 30, 73 View image mode, 45, 62 Weight, 66, 113 Amersham TYPHOON Operating Instructions 29193226 AF...
  • Page 136 Cytiva and the Drop logo are trademarks of Life Sciences IP Holdings Corp. or an affiliate doing business as Cytiva. Amersham, and Typhoon are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.

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