Summary of Contents for Roper Scientific PHOTOMETRICS CoolSNAPHQ2
- Page 1 sales@artisantg.com artisantg.com (217) 352-9330 | Visit our website - Click HERE...
- Page 3 User Manual for CoolSNAP and CoolSNAP Systems 57-063-001 Rev D0...
- Page 4 Photometrics, a division of Roper Scientific, Inc. Printed in the United States of America. CoolSNAP and RS Image are trademarks and Photometrics, PVCAM, and Roper Scientific are registered trademarks of Roper Scientific, Inc. Linux is a registered trademark of Linus Torvalds.
- Page 5 LIMITED WARRANTY Photometrics, a division of Roper Scientific, Inc., (“Photometrics,” us,” “we,” “our”) makes the following limited warranties. These limited warranties extend to the original purchaser (“You”, “you”) only and no other purchaser or transferee. We have complete control over all warranties and may alter or terminate any or all warranties at any time we deem necessary.
- Page 6 Your Responsibility The above Limited Warranties are subject to the following terms and conditions: You must retain your bill of sale (invoice) and present it upon request for service and repairs or provide other proof of purchase satisfactory to Photometrics. You must notify the Photometrics factory service center within (30) days after you have taken delivery of a product or part that you believe to be defective.
- Page 7 U. S. Government Restricted Rights The software and documentation are provided with Restricted Rights. Use, duplication, or disclosure by the Government is subject to restrictions as set forth in subparagraph (c)(1)(ii) of the Rights in Technical Data and Computer Software clause at DFARS 252.227-7013 or subparagraphs (c)(1) and (2) of the Commercial Computer Software-Restricted Rights at 48 CFR 52.227-19, as applicable.
- Page 9 viii CoolSNAP /CoolSNAP User Manual...
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Page 10: Table Of Contents
Table of Contents Chapter 1. Introduction Description ............................1 System Components........................1 About This Manual......................... 2 Precautions............................2 Environmental Requirements....................... 3 Storage Requirements ........................3 Microscopes, Lenses, and Tripods....................3 Repairs .............................. 3 Cleaning............................3 Photometrics Customer Service ....................4 Chapter 2. - Page 11 Chapter 5. Basic Specifications Camera Dimensions (Front) ......................15 Camera Dimensions (Bottom).....................16 Focal Plane Measurement......................17 Additional Measurements ......................18 CCD Specifications and Orientation..................18 Connectors............................19 POWER Connector Pinout....................19 I/O Connector Pinout......................20 Power Supply Specifications and Dimensions ................22 Figures Figure 1. Normal Mode, Overlapped ..................9 Figure 2.
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Page 12: Chapter 1. Introduction
® The Photometrics CoolSNAP and CoolSNAP , from Roper Scientific are industry-leading cameras for low-light fluorescence microscopy. These cameras incorporate progressive-scan CCDs (charge-coupled devices), a 12- or 14-bit digitizer, and low-noise electronics to produce monochrome images at greater than 1k x 1k resolution. -
Page 13: About This Manual
About This The CoolSNAP User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper Manual use. The chapters that follow this introduction are System Installation — Instructions for connecting your CoolSNAP •... -
Page 14: Environmental Requirements
Environmental The CoolSNAP camera system should be operated in a clean, dry environment. Requirements The camera system’s ambient operating temperature is 0°C to 30°C with less than 80% relative humidity, noncondensing. Storage Store the CoolSNAP camera system in its original containers. To protect the system from excessive heat, cold, and moisture, store at an ambient temperature Requirements between -20°C and 60°C with a relative humidity of 0%-90%, noncondensing. -
Page 15: Photometrics Customer Service
In Europe, you can reach Customer Service at: BENELUX Tel: 31.347.324989 • Fax: 31.347.324979 • Email: mailto@roperscientific.com • Mail: Roper Scientific, BV • Ir. D.S. Tuijnmanweg 10 4131 PN VIANEN, Netherlands FRANCE Tel: 33.160.86.03.65 • Fax: 33.160.86.07.09 • Email: princeton.instruments@wanadoo.fr •... -
Page 16: Chapter 2. System Installation
System Installation Chapter 2. Carefully review the Precautions section on page 2 before performing any of the procedures outlined here. Again, use only a CoolSNAP cable and a CoolSNAP interface card with your CoolSNAP camera. Using a different cable or interface card may result in permanent damage to your system. -
Page 17: Multiple Cameras
If you are a Mac user, the host computer for your CoolSNAP camera must have the following: Macintosh OS X.3 (Panther) • 512 MB RAM (or greater) • CD-ROM drive • At least one unused PCI or PCI-X card slot (LVDS cameras only) •... -
Page 18: Connecting Your Coolsnap Camera
Connecting Your The CoolSNAP cable connects your CoolSNAP camera to the CoolSNAP interface card. It is designed to serve as a conduit for data. CoolSNAP Camera CoolSNAP IEEE-1394 Cable CoolSNAP LVDS Cable To connect your CoolSNAP LVDS camera: Connect either end of the CoolSNAP LVDS cable to the CoolSNAP interface card that you have installed in the host computer. - Page 19 To connect your CoolSNAP IEEE-1394 camera: Select the appropriate CoolSNAP IEEE-1394 data cable: If you installed the CoolSNAP IEEE-1394 interface card, or have • another IEEE-1394 interface card already installed, use the 6-pin to 6- pin data cable. If you have a 4-pin IEEE-1394 connector on your computer (such as a •...
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Page 20: Chapter 3. Operating Features
Operating Features Chapter 3 Dual-Mode A unique feature of the CoolSNAP is that it allows two different CCD Operation clocking modes that allow you choose between speed and NIR sensitivity. In the PVCAM implementation, the clocking modes are referred to as "normal" and (CoolSNAP Only) "alternate normal". -
Page 21: Alternate Normal Mode
Alternate Normal mode In "Alt-Normal", it is possible to achieve higher QE in the NIR (peak QE of approximately 65%) by manipulating the CCD clock voltages. Also, in this high-sensitivity mode, the preamplifier is switched off during the exposure to eliminate the background generated by preamplifier glow. -
Page 22: Trigger-First Mode
Trigger-first mode In this mode, the camera requires only one trigger to acquire a sequence of frames. Each frame is exposed for a length of time entered into the software and read out. Once the trigger is received, the camera is inhibited from taking any further triggers until the entire sequence is completed (see Figure 4). -
Page 23: Coolsnap Hq
CoolSNAP Application Examples Example 1 "I have a CoolSNAP and want to operate the camera in the most sensitive setting for taking high-resolution, single images." For this application, the camera should be operated in "alternate normal" mode to provide the best quantum efficiency. Furthermore, the readout speed of the camera should be set to 10 MHz to reduce the read noise. -
Page 24: Chapter 4. Troubleshooting
Troubleshooting Chapter 4. If you have any difficulty while troubleshooting, or do not see your camera system’s symptoms listed here, contact Photometrics Customer Service. System Does Not If your operating system does not boot normally after you have installed an interface card, try installing the new card in another open PCI slot. -
Page 25: Bright Spots In Image / Increased Background Noise
Bright Spots in If you notice bright spots (hot pixels) in the image or an increase in background noise, take another calibration image and then re-acquire the original image. Image / Increased Background Noise Camera Running It is normal for the camera to be slightly warm to the touch while in operation. However, if the camera is more than slightly warm to the touch (and at least 1/2 Too Warm inch of space has been left around the camera for air flow), switch off the camera... -
Page 26: Chapter 5. Basic Specifications
Basic Specifications Chapter 5. Camera Dimensions 4.50 in (11.43 cm) (Front) 4.00 in 4.30 in (10.16 cm) (10.92 cm) CoolSNAP Camera: Front View 4.50 in (11.43 cm) (2) Holes .15 in (.38 cm) dia x Pin 1 .15 in (.38 cm) deep Orientatio 4.00 in (10.16 cm) -
Page 27: Camera Dimensions (Bottom)
Camera Dimensions (Bottom) 2.90 in (7.37 cm) 7.01 in (17.8 cm) 7.19 in (18.26 cm) CoolSNAP Camera: Bottom View CoolSNAP Camera: Bottom View CoolSNAP /CoolSNAP User Manual... -
Page 28: Focal Plane Measurement
Focal Plane Measurement .66 in (16.8 mm) FOCAL PLANE CoolSNAP Camera: Focal Plane CoolSNAP Camera: Focal Plane Chapter 5. Basic Specifications... -
Page 29: Additional Measurements
Additional Camera weight: 6.5 lb. (2.9 kg) Measurements Tripod mounting hole (bottom of camera): .25 in.-20 UNC-2B Safe tripod mounting screw length: .25 in. (.63 cm) Flange focal distance: .66 in (16.8 mm) CoolSNAP CoolSNAP Specifications and Orientation Image Type Mono Mono Resolution... -
Page 30: Connectors
Connectors CoolSNAP (IEEE-1394 version) CoolSNAP (LVDS version) Rear Panel CoolSNAP Rear Panel The following connectors are located on the back of the camera: DATA connector: for data transfer. LVDS cameras: 20-pin, high-density connector. • IEEE-1394 cameras: 6-pin, Type 1 IEEE-1394 connector. •... -
Page 31: I/O Connector Pinout
I/O Connector Pinout The I/O (Input/Output Status) connector provides information about trigger function, DAC, and TTL signals. Inputs must be at least 3.15 V for a high and less than 0.9 V for a low. The numbers on the I/O connector diagram correspond to the numbers given to the definition of each of the pins. - Page 32 12 TTL I/O data bit 0: TTL level programmable input or output Port 1 13 TTL I/O data bit 1: TTL level programmable input or output 14 TTL I/O data bit 0: TTL level programmable input or output Port 2 15 TTL I/O data bit 1: TTL level programmable input or output 16 TTL I/O data bit 0: TTL level programmable input or output Port 3...
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Page 33: Power Supply Specifications And Dimensions
Power Supply Voltage Output: +12 Vdc Specifications Voltage Input: 100-240 V~ @ 47-63 Hz Dimensions Maximum Power Output: 110 W Power Supply Weight: 4.5 lb (2.0 kg) 2.70 in (69.0 mm) 5.00 in (127.0 mm) Power Supply: Front View 5.00 in (127.0 mm) 10.00 in (254.0 mm) - Page 34 BENELUX FRANCE GERMANY Roper Scientific, BV Roper Scientific, SARL Roper Scientific, GmbH Ir. D.S. Tuijnmanweg 10 Z.I. Petite Montagne Sud Rosenheimer Landstr. 87 4131 PN VIANEN, Netherlands 4, rue de l'Oisans - C.E. 1702 D-85521 Ottobrunn, Germany tel: 31.347.324989 91017 Evry Cedex, France tel: 49.89.660.779.3...
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