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OPERATING INSTRUCTIONS AND
SYSTEM DESCRIPTION FOR THE
PDES-02DX
PNEUMATIC DRUG EJECTION
SYSTEM
(with digital timers and digital manometers – PDES-02DX-LA)
VERSION 2.0
npi 2013
npi electronic GmbH, Bauhofring 16, D-71732 Tamm, Germany
Phone +49 (0)7141-9730230; Fax: +49 (0)7141-9730240
support@npielectronic.com; http://www.npielectronic.com
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Summary of Contents for NPI PDES-02DX
- Page 1 OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE PDES-02DX PNEUMATIC DRUG EJECTION SYSTEM (with digital timers and digital manometers – PDES-02DX-LA) VERSION 2.0 npi 2013 npi electronic GmbH, Bauhofring 16, D-71732 Tamm, Germany Phone +49 (0)7141-9730230; Fax: +49 (0)7141-9730240 support@npielectronic.com; http://www.npielectronic.com...
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Page 2: Table Of Contents
PDES-02DX-LA User Manual ___________________________________________________________________________ Table of Contents 1. Safety Regulations......................3 2. PDES-2DX System ......................4 2.1. System Description...................... 4 2.2. Description of the Front Panel..................5 2.3. Description of the Rear Panel..................7 2.4. External Valves ......................8 Remote Valve Plate ..................... 8 microJECT micro Valve Pipette Holder.............. -
Page 3: Safety Regulations
Use only dry, oil free, filtered gases (max. 5 µm filter is allowed). Maximum input pressure must be below 5 bar (1 bar = 100,000 Pa). npi recommends an input pressure of 1 bar more than the maximum output pressure. -
Page 4: Pdes-2Dx System
"retain" negative pressure (analogous to the "retaining" current used in iontophoretic systems). The "retain" pressure must be generated by an additional pneumatic device. Please contact npi electronic for details. The injection pressure is preset by a precision pressure regulator. The pressure is displayed on a digital manometer. -
Page 5: Description Of The Front Panel
PDES-02DX-LA User Manual ___________________________________________________________________________ 2.2. Description of the Front Panel Figure 1: PDES-2DX front panel view (the numbers are related to those in the text below) ___________________________________________________________________________ version 1.3 page 5... - Page 6 PDES-02DX-LA User Manual ___________________________________________________________________________ In the following description of the front panel elements, each element has a number that is related to that in Figure 1. The number is followed by the name (in uppercase letters) written on the front panel and the type of the element (in lowercase letters). Then, a short description of the element is given.
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Page 7: Description Of The Rear Panel
(3) SOURCE IN connector Connector for connecting the filtered, dry and oil-free gas source (5 bar max.). Note: npi recommends an input pressure of 1 bar more than the maximum output pressure. (4) FUSE Unplug the instrument when replacing the fuse or changing line voltage. Turn knob to open. -
Page 8: External Valves
Remote Valve Plate The valves which are controlled by the PDES-02DX are located on the remote valve plate (see Figure 3). Tubing and cable of the external valves are connected at the rear panel of the PDES system (refer to chapter 2.3). -
Page 9: Microject Micro Valve Pipette Holder
___________________________________________________________________________ microJECT micro Valve Pipette Holder The valve which is controlled by the PDES-02DX is located in the micro valve pipette holder, called microJECT (see Figure 4). Tubing and cable of the microJECT are connected at the rear panel of the PDES system (refer to chapter 2.3). Exhaust is usually not connected. -
Page 10: Operation
PDES-02DX-LA User Manual ___________________________________________________________________________ 2.5. Operation Connect the gas source to the SOURCE IN connector at the rear panel. The gas source must be clean and filtered by a 5 µm filter element. Important: Do not connect sources of OXYGEN or COMBUSTIBLE or EXPLOSIVE... - Page 11 PDES-02DX-LA User Manual ___________________________________________________________________________ REMOTE CONTROL (option): The selected operation mode (at the controller) determines the way the REMOTE CONTROL works. In the CONT. mode the remote control unit is disabled. In the EXTERN mode the valve is open as long the REMOTE control switch is activated (CONT./TIMED = continuous, START = temporarily).
- Page 12 PDES-02DX-LA User Manual ___________________________________________________________________________ Figure 8: operation modes of the PDES-2DX ___________________________________________________________________________ version 1.3 page 12...
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Page 13: Literature
PDES-02DX-LA User Manual ___________________________________________________________________________ 3. Literature General Methods o Hahnel, C. (1992) Quantitative Microinjection, in: H. Kettenmann and R. Grantyn (eds.) Practical Electrophysiological methods, Wiley-Liss, New York. o Hofmeier G, Lux HD (1981) The time course of intracellular free calcium and related electrical effects after injection of CaCl into neurons of the snail, Helix pomatia Pflügers... -
Page 14: Microinjection Into Oocytes
PDES-02DX-LA User Manual ___________________________________________________________________________ o Ponimaskin, E., Dumuis, A., Gaven, F., Barthet, G., Heine, M., Glebov, K., Richter, D. W., & Oppermann, M. (2005). Palmitoylation of the 5-Hydroxytryptamine4a Receptor Regulates Receptor Phosphorylation, Desensitization, and {beta}-Arrestin-Mediated Endocytosis. Molecular Pharmacology 67, 1434-1443. -
Page 15: Technical Data
PDES-02DX-LA User Manual ___________________________________________________________________________ 4. Technical Data Source gas: air, nitrogen, helium etc. no aggressive, combustible or explosive gases, filtered by a 5 µm filter element. Pressure range: input pressure: max. 5 bar (1 bar = 10 Pa = 14.5 psi)
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