Summary of Contents for DELTAVISION OMX SR
- Page 1 DeltaVision OMX SR (SIM and TIRFM) User manual Cell Imaging & Cytometry CONTACT: microscopy@bioscience.fi +358 (0)20 7032561(office) 050 369 7532 (mobile) Turku Bioscience 5th floor, room 5077...
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Page 2: Table Of Contents
Contents Information ........................1 Before Imaging ......................2 Working with immersion objectives ................3 Start-up and standby ...................... 4 Start-up ........................4 Standby mode ......................4 Starting the software ...................... 5 Imaging settings (Light parameters) ................5 TIRFM ..........................7 Image processing ...................... -
Page 3: Information
1. Information The Cell Imaging and Cytometry Core is responsible for the maintenance of this microscope. Each user must follow the CIC user policy. More information: https://bioscience.fi/services/cell-imaging o Only authorized users may use the CIC instruments. o Users must report any malfunction or problem to the CIC personnel. -
Page 4: Before Imaging
2. Before Imaging with an ordinary fluorescence microscope Check your sample It is a good practice to check the quality of your sample before making use of a more expensive instrument. Clean your slides Clean the remaining salt and mounting medium off the coverslip. Dirty coverslip compromises image quality. -
Page 5: Working With Immersion Objectives
3. Working with immersion objectives Please follow our common rules on how to work with immersion objectives, but note the special rules concerning the OMX: The immersion medium should match a sample refractive index and used laser lines. Use the Immersion Oil Calculator application by Ge Healthcare to find a correct immersion oil (selection of 15 grades with refractive indices between 1.510 and 1.534): https://www.gelifesciences.com/en/us/support/online-tools/cell-imaging-and-... -
Page 6: Start-Up And Standby
4. Start-up and standby 1. Start-up 1. Switch on the lasers you need, and turn ON the laser interlock key. 2. Switch on the monitors 3. Start the OMX software 4. Ensure that the correct objective is installed (Objectives can be changed only by CIC personnel) * 60x 1.42 SI objective * 60x 1.49 TIRF objective (check the temperature collar!) 5. -
Page 7: Starting The Software
5. Apply an immersion oil type 6. Place the sample 6. Imaging settings (Light parameters) See the DeltaVision OMX Quick reference 1, Startup and Acquisition to find more information on loading a sample, navigation, defining the light parameters and experiment settings. - Page 8 Focusing: Use Ultra Focus system (UF) to find the sample surface. Continue focusing with small Z-steps to find the sample (dZ 1-5 um depending on a sample). Fine-tune a focus by using step size 0.1 - 0.5 um. Navigation in XY Use arrow steps, or drag and drop the navigation square, or “go to position”...
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Page 9: Tirfm
(from the area of max 5x5 or 10x10 frames). Note that too much oil can cause similar drifting problems. 8. Image processing Please follow instructions from the DeltaVision OMX Quick reference 2, SI Image Reconstruction. -
Page 10: Troubleshooting
Troubleshooting 1. The stage does not work Known issues: 1. The stage does not travel to the expected position (x.y.z) 2. The stage can travel in the z-axis but does not reach the correct level Solution: 1. Remove the sample and sample holder 2.
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