Table of Contents
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Table of Contents
Summary of Contents for StatSpin ThermoBrite S500-12
- Page 1 Service Manual...
- Page 2 Service Manual ThermoBrite Model Number S500-12/S500-24 FOR IN VITRO DIAGNOSTIC USE StatSpin is a registered trademark of StatSpin Inc., a Subsidiary of IRIS International, Inc. Copyright 2004 Printed in U.S.A.
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Page 3: Table Of Contents
Table of Contents Table of Contents......................i How to use this manual..................iii Section 1 ......................1 Safety..........................1 Before you Begin...................... 1 Install System......................1 Connect Power ......................1 Section 2 ......................2 System Overview ......................2 Principle and Intended Use ..................2 Symbols and Definitions.................. - Page 4 Overview........................15 Character Selection ....................15 Creating a Denaturation and Hybridization Program (Denat & Hyb) ..... 15 Section 8 ......................16 Schematic........................16 Block Diagram ......................16 Main PCB......................... 17 Main PCB......................... 18 Section 9 ......................19 Disassembly ........................19 Heater Assembly ....................19 Housing Assembly ....................
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Page 5: How To Use This Manual
How to use this manual This manual along with information contained on product labels should provide you with all the information you need to operate and maintain the ThermoBrite. Notes appear in italics to highlight information. When the information requires special attention, a caution symbol appears next to the italicized text. -
Page 6: Section 1
This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. You introduce software that is not authorized by StatSpin into the instrument. Only operate your system with software authorized by StatSpin. -
Page 7: Section 2
Move cursor back or previous screen Stop End a program in process Enter numeric values for time and temperature or for program name Product/Reference Indicates the StatSpin product/catalog number Number Caution Statement of caution/warning, read instruction carefully Temperature Indicates storage requirements range... -
Page 8: Symbols And Definitions (Cont.)
Symbols and Definitions (cont.) Manufactured By Indicates Manufacturer of the Device Electric Shock Indicates a potential risk of electrical shock Biohazard Universal Precautions should be followed at all times Hot Surface Use caution to avoid burn Display Abbreviations Display Abbreviations Program Denat &... -
Page 9: Error Messages
Error Messages Instrument cannot achieve a set temperature by heating within 10 minutes an error message appears to inform user to turn unit off and call service. A constant beep will sound. If the instrument cannot measure the temperature, the software will automatically turn off the heating. An error message will appear to inform the user to turn off the unit and call service. -
Page 10: Section 3
Section Operating Instructions Opening and Closing the Lid The platen may be hot. Use caution and check temperature on display before handling slides. Improper precaution can cause a burn. Depressions located on either side of the lid allow user to simply lift lid into position. The lid should offer some resistance when opening. - Page 11 Denaturation and Hybridization: Display indicates present temperature of the slides. Once temperature reaches denaturation set point, ThermoBrite will beep twice and denaturation time will count down from the set time. PGM 01 Her2 Denat in Process Denat: 82°C 02:28 Present Temp: 82°C The ThermoBritewill automatically cool to hybridization set temperature once denaturation is completed.
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Page 12: Abort Program In Process
PGM 03 Appl Fixed Temp: 65°C Reset Timer 01:18:10 End PGM/Main Menu Use Arrow keys to move to “End PGM/Main Menu” line and press “Enter” button to accept. Note: If ambient temperature is programmed the fan will continually cycle until the program is aborted. -
Page 13: Section 4
Section Specifications Table Product No. TS01 TS02 Model No. S500-12 S500-24 Capacity Up to 12 slides Processing Time 0-100 hours Number of Programs Ramp Time 37 – 95ºC in less than 3 minutes Cooling Time 95 - 45ºC in less than 6 minutes Electrical 120 VAC @ 3.0 A 240 VAC @ 1.6A... -
Page 14: Section 5
Section Theory of Operation Overview The ThermoBrite is a programmable device that is used to control a plate upon which samples are placed to precision temperatures. The device is required to move quickly between various set temperatures with little or no overshoot and then remain at the set temperature within +-1 degree for any desired time. -
Page 15: Pid
The system uses a PID algorithm to get quick and precise control of temperatures over the usable range of the instrument. If we define a temperature error (E) as the difference between the temperature setpoint and the measured temperature, a standard PID control would involve the following equation: Control = Pg*E + Ig*∫E *dt+Rg*dT/dt Where: Pg is a constant (proportional gain) -
Page 16: Section 6
Select “H” to access the help screen. Note: This screen will provide a list of options. Select “S” for sign on and choose either Dako for Hybridizer or StatSpin for ThermoBrite. Select “L” to cycle through the languages and select the “English” version. -
Page 17: Calibration
6) Heating rate deadband: 15.0 7) Cooling rate deadband: 1.0 8) Intergrator max value: 1000.0 9) Heating cycle time 500 ms A) Cooling cycle time 1000 ms B) Duty cycle deadband 0.30 X) Ambient cycle time 2000 ms Y) Ambient duty fraction 0.05 Z) Use offset: Yes C) Setpoint: XX.X C D) Defaults... -
Page 18: Testing
Note: The voltage will be listed on the device serial number label. Turn on the ThermoBrite unit. The Name “DakoCytomation” (Hybridizer) or “StatSpin Inc.” (ThermoBrite) and the software revision will appear on the display for a short time. Note: The prompt screen will supersede this screen. - Page 19 Note: It will beep once it reaches 95.0°C. Start the timer and record the temperature readings at 2, 5, and 10-minute intervals for the six probes. Note: The 95.0°C test readings should be between 94.0°C and 96.0°C. The unit passes the test if the readings are within range.
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Page 20: Section 7
Section Programming Overview ThermoBrite is capable of storing 40 different programs. Each program can be one of three program types: Denaturation and Hybridization (Denat & Hyb), Hybridization Only (Hyb Only) or Fixed Temperature (Fixed Temp). Select PGM Type Denat & Hyb Hyb Only Fixed Temp Programming is simple. -
Page 21: Section 8
Section Schematic Block Diagram R TD E M I F ILTER H E ATER P O W ER... -
Page 22: Main Pcb
Main PCB... -
Page 23: Main Pcb
Main PCB... -
Page 24: Section 9
Section Disassembly Heater Assembly Prior to disassembly, Unplug unit from power source. Turn unit on its side to expose the underside of the instrument. Locate (4) black screws. Remove screws identified in Figure 1 for plenum mount. FIGURE 1 TURN THE UNIT TO ITS UPRIGHT POSITION. REMOVE PLENUM BY LIFTING STRAIGHT UP TO CEAR THE MOUNTING STANOFFS. -
Page 25: Housing Assembly
Housing Assembly LIFT HOUSING ASSEMBLY UP UNTIL IT CLEARS THE BACK END OF CHASSIS. TILT BACK END OF HOUSING UP AND TOWARD MAIN PC BOARD AND DISCONNECT THE PIGTAILS OF THE DISPLAY AND KEYPAD ASSEMBLIES. SEE FIGURE 3 FIGURE 3... -
Page 26: Section 10
Ensure lid is properly closed. Ensure air intake and outlet are not obstructed. Cannot read Display Allow unit to reach room temperature before operating. Contact StatSpin Customer Service. Temperature on display Clean slide heating plate, replace with clean slides. does not match surface... -
Page 27: Section 11
Section Exploded View ThermoBrite... -
Page 28: Part Numbers
LINE CORD (120) 01-004987-001 RS232 MANUAL 01-004861-001 RS232 KIT 01-004988-001 OP.MANUAL 01-004997-001 BOX WITH FOAM SET 01-004983-001 FOAM SET A Subsidiary of IRIS International ,Inc Norwood, MA 02062 USA Phone: 800-782-8774 or 781-551-0100 Fax: 781-551-0036 www.statspin.com 55-005013-001 Rev. A Made in USA...
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