Enzo Absorbance 96 Product Manual
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Enzo Absorbance 96 Product Manual

Plate reader
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Product Manual
Absorbance 96 plate reader
Catalog #: ENZ-INS-A96
Manufactured by Byonoy GmbH
Device Version 2.0 (2021-01-18)
Software Version 1.2 (2021-01-18)
For the latest product information, including support documentation, visit us
online:
www.enzolifesciences.com
Technical Support (US): 800-942-0430
Technical Support (EU): +41 61 926 8989
Customer Service (US):
info-usa@enzolifesciences.com
Customer Service (EU):
info-eu@enzolifesciences.com
FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Rev 052021

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Summary of Contents for Enzo Absorbance 96

  • Page 1 Product Manual Absorbance 96 plate reader Catalog #: ENZ-INS-A96 Manufactured by Byonoy GmbH Device Version 2.0 (2021-01-18) Software Version 1.2 (2021-01-18) For the latest product information, including support documentation, visit us online: www.enzolifesciences.com Technical Support (US): 800-942-0430 Technical Support (EU): +41 61 926 8989 Customer Service (US): info-usa@enzolifesciences.com...
  • Page 2 Instructions for Use Absorbance 96 Valid for REF Number ABS MH A 01/ABS MH D 01 Instructions for Use Absorbance 96 Version 2.0 (18/01/2021) ©Copyright 2021 by Byonoy GmbH. All rights reserved. Manufacturer: Byonoy GmbH 00 49 40 5379 866 00 Bernstorffstr.
  • Page 3 Dear customer, We are delighted that you have chosen the Absorbance 96. To take full advantage of the instrument's performance and to enjoy your instrument for many years, please read these instructions for use carefully before installation and commissioning. Operate the instrument in accordance with these instructions.

  • Page 4: Table Of Contents

    TABLE OF CONTENTS General information ........................1 Overview of the Absorbance 96 ..................3 2.1 Scope of application ..........................3 2.2 Measurement method ..........................3 2.3 Absorbance 96 System ........................... 3 2.4 Consumables ..............................5 Preparing the product for use ....................6 3.1 Unpacking, storage, and transport ....................

  • Page 5: General Information

    General information General information Important: Follow the instructions for use Any commissioning or handling of the instrument requires precise knowledge and notice of this manual. The device is intended for the described use only. In this manual, particularly important remarks are labeled as followed: Warning: This is a warning that indicates an immediately or potentially dangerous situa- tion, which, if not avoided, will result in death or serious injury.
  • Page 6 Working with biological and harmful material The Absorbance 96 is not to be used for the measurement of biohazardous substances. Always observe the manufacturer's hazard information pertaining to the substances to be measured.

  • Page 7: Overview Of The Absorbance 96

    Absorbance 96 System Absorbance 96 Reader The Absorbance 96 reader is an absorbance-based measuring device, i. e. a measuring in- strument, with which the absorbance values of a 96-well microtiter plate can be read, rec- orded and provided for further processing. The Absorbance 96 is a solid-state microplate...
  • Page 8 Figure 1. Visual representation of the product from the front, top. 1. Signal lights. 2. Slot for microtiter plate. 3. Raised locking-edges. 4. Arrow. On the back of the Absorbance 96 there is a Micro-USB port for connecting the instrument to a computer via the included Micro-USB cable. Furthermore, there is an eject button for ejecting the microtiter plate out of the slot.

  • Page 9: Consumables

    Overview of the Absorbance 96 The reader is controlled via the Absorbance 96 App. The operation of the app is described in-detail in a separate user manual. Consumables With the Absorbance 96, ANSI/SBS Standard 96-well microtiter plates can be used. Only certain microplate types can be used with the Absorbance 96 (see chapter 7).

  • Page 10: Preparing The Product For Use

    When unpacking the device, please check that the following components are present: • Absorbance 96 • Micro-USB cable • Hex key • Microfiber cloth • USB Stick Working environment Due to its small size, the Absorbance 96 can be operated very well in various working envi- ronments.
  • Page 11 Preparing the product for use However, the following warnings must be observed. Ignoring them may lead to measurement errors and a reduction in the expected lifetime or damage to the device: The device should stand on a level surface, free from dust and vibrations. Do not operate the device near heat sources or under direct sunlight.

  • Page 12: Operation

    The functions of the app are detailed in a separate user manual. App installation procedure and updates The app is compatible with Windows und MacOS operating systems (see chapter 7). To install the Absorbance 96 App, insert the USB stick into the computer and follow the steps below: •...

  • Page 13: Assay Readout

    Insert the plate cautiously to avoid spills. After a successful measurement, the results section will open automatically in the app. The microtiter plate may now be removed from the Absorbance 96. To do this, push the eject button on the rear side of the device.

  • Page 14: Signals And Troubleshooting

    Signals and troubleshooting Signals and troubleshooting Signal lights Table 1. Explanation of the signal lights Activity of the signal light Meaning All signal lights flash simultaneously Error, software shows error message Device is off No signal lights light up Device is waiting for input Device is defective All signal lights light up briefly in sequence Self-test after connection to power...
  • Page 15 Signals and troubleshooting Irrecoverable hard- Irrecoverable hardware Contact the service department. ware error. error.

  • Page 16: Maintenance And Cleaning

    The Absorbance 96 is maintenance-free. Each time the instrument is switched on, an internal self-test is carried out to ensure there are no malfunctions. There are no parts within the Absorbance 96 that can be serviced by the customer. It is only necessary to ensure that the device is kept clean.

  • Page 17: Cleaning

    Do not use scouring agents. Cleaning the slot To clean the slot of the Absorbance 96 open the four screws on the bottom of the instrument (see Figure 3) using the Hex key provided.
  • Page 18 Maintenance and cleaning Figure 3. Bottom side of the instrument Figure 4. Pull the instrument apart into its two components Carefully pull apart the top and the bottom of the instrument to separate the plug connection on the back of the instrument. To avoid damaging the rigid plug connection between the top and bottom of the instrument, grasp the instrument at the back during the pull movement and avoid any tilting movement.

  • Page 19: Technical Support

    This can lead to a reduction of the service life or in measurement errors: Always avoid spraying liquid directly onto the surfaces of the Absorbance 96. This is especially important for the inside of the slot, where there are optical el- ements that are very sensitive and essential for the functioning of the instru- ment.

  • Page 20: Technical Specifications

    Technical specifications Technical specifications Table 3. Performance and technical data Parameter Value Type of product Absorbance reader Product name Absorbance 96 Service life 10 years at an average use of 4h/day Housing material Aluminium Place for use Laboratory Degree of contamination Temperature for storage/measurement 5–40 °C...
  • Page 21 Technical specifications Nominal value/Characteristic of the fuse 1 A/very fast-acting Power input 2.5 W Weight 900 g Microsoft Windows: Windows 7 or above System requirements (App) Mac OS: High Sierra 10.13 or above *Accuracy is the maximum deviation between the determined value and the true value. **Reproducibility is the maximum deviation between the determined values when the measurement is repeated directly.

  • Page 22: Guarantee

    Guarantee Guarantee The regular warranty period of the Absorbance 96 is 24 months. If a defect manifests itself in your device during the warranty period, please contact the service department directly. The instrument may only be operated in technically perfect condition. In the event of defects that could endanger employees or third parties, the device may only be used again after it has been repaired by the manufacturer.

  • Page 23: Ec-Conformity Declaration

    EC-Conformity Declaration EC-Conformity Declaration...
  • Page 24 Instructions for Use Absorbance 96 App Version 1.2 Instructions for Use Absorbance 96 App Version 1.0 (18/01/2021) ©Copyright 2021 by Byonoy GmbH. All rights reserved. Manufacturer: Byonoy GmbH 00 49 40 5379 866 00 Bernstorffstr. 118 service@byonoy.com 22767 Hamburg, Germany...
  • Page 25 TABLE OF CONTENTS General information ........................1 App installation procedure and updates ................ 2 Absorbance 96 App overview ....................3 Operation ............................5 4.1 Set up an assay and create a protocol ..................5 4.2 Readout of the assay ..........................8 4.3 Results ..................................

  • Page 26: General Information

    General information General information The Absorbance 96 App is to be used only in combination with the Absorbance 96 reader. The Absorbance 96 App is not a stand-alone product. Please also refer to the separate op- erating instructions for the Absorbance 96 reader.

  • Page 27: App Installation Procedure And Updates

    The Absorbance 96 App is compatible with Microsoft Windows (Windows 7 or above) and Mac OS (High Sierra 10.13. or above). To install the Absorbance 96 App, insert the USB stick into the computer and follow the steps below: •...

  • Page 28: Absorbance 96 App Overview

    Absorbance 96 App overview Absorbance 96 App overview The Absorbance 96 App is used to operate the Absorbance 96 reader and process the re- sults. After the app is started and the reader is connected, the main window appears auto- matically.
  • Page 29 Absorbance 96 App overview File Types The following file types are used by the Absorbance 96 App: Table 1. File Types in the Absorbance 96 App Description Extension Protocol .byop Assay .byoa Export .csv/.pdf The protocol file type .byop allows to save settings and to load predefined protocols for re- peat assays.

  • Page 30: Operation

    Operation Operation Set up an assay and create a protocol Select “New” from the menu bar. Your experiment conditions can now be defined. Workflow Parameters Wavelengths Under “Wavelengths”, a signal and reference wavelength (both in nanometers) may be cho- sen. The signal wavelength is the wavelength at which the principal measurement will be run.
  • Page 31 Operation Predefined standard curve In a quantitative assay, a predefined standard curve can be used to determine the concen- trations of a new set of samples with identical experiment parameters. After selecting the checkbox “Use predefined standard curve”, you may enter the curve parameters manually or load a standard curve from a previous assay.
  • Page 32 Operation Example: To replace the minus function, the user must simply enter a negative value. For example, if the cut-off is Cut-off = Mean Negative Controls - 0.2, this must be entered as follows: Figure 3. Cut-off calculation with subtraction In case the Cut-Off is known or provided by the kit manufacturer, and therefore does not need to be calculated, select 0 OD and enter the value into the middle field: Figure 4.

  • Page 33: Readout Of The Assay

    Operation Create a protocol Once the parameters of a given experiment have been set, these settings may be saved as a protocol. All parameters, such as wavelength and plate layout, will be saved. Readout of the assay Once all settings have been completed, the read out can be started. The app will automati- cally guide the user through the readout process.

  • Page 34: Ec-Conformity Declaration

    EC-Conformity Declaration EC-Conformity Declaration...

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Enz-ins-a96

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