Life Technologies EVOS FL Auto - fluorescence Microscope Quick Reference

The EVOS^® FL Auto Imaging System is a fully automated imaging system controlled by the integrated EVOS^® FL Auto software. The software, accessed by the touch-screen monitor, controls the automated X-Y axis stage and the objective turret, and features standard and advanced imaging and analysis tools.

Note: The EVOS^® FL Auto Imaging System User Guide is provided on the USB fl ash drive. You can also dowload it from the EVOS^®FL Auto Imaging

System product page at www.lifetechnologies.com.

Home page of the EVOS^® FL Auto software

• Quick Image: Provides step-by-step instructions for capturing an image in the fastest way possible.
• Capture: Captures a single image while allowing the direct control of the instrument.
• Scan: Scans an area to acquire multiple images to build a tiled and stitched image.
• Time Lapse: Creates and runs time lapse movies based on user specifi cations.

Basic Operation - Quick Image

Note: For more detailed information such as advanced operations (i.e., editing images, creating scan routines, time lapse imaging, etc.) refer to the EVOS^® FL Auto Imaging System User Guide.

1. Place the sample on the stage using the appropriate vessel holder.
2. On the Home page, click Quick Image.
3. Using the Quick Image Wizard, enter the following information:

• Which Vessel Œ: Select vessel type
• Where to Start : Select a location to view
• Which Lighting : Select the light paths for preview and capture (you can select multiple paths for the capture overlay)
• What Magnification: Select the objective(s) to preview and capture the image(s)
  

4. Click Launch Wizard! . The system will move to the ImageCapture tab, which will display the preview choices selected in Step 3.
5. Adjust the focus and illumination using the Light ‘ and Focus sliders ’. The Light Path buttons “ will allow adjustments to each individual channel.
   
6. Press Preset ” to utilize the capture options selected in Step 3. The system will automatically move to the Image EditOverlay tab to allow for further adjustments to brightness and contrast •.
7. Click Save Image . For more information, refer to the EVOS^® FL Auto User Guide.
   

Capture Tab Controls

The controls on the Capture tab can be accessed via the Quick Image Wizard (see Basic Operation - Quick Image), by selecting Capture on the Home page, or by clicking the ImageCapture tab on any screen.

• Vessel ExpertŒ: Used for selecting vessel type; the crosshairs on the virtual vessel are used for choosing a location to view.
• Objective buttons : Automatically adjust the objective turret for the desired magnifi cation.
• Power button: Turns the light ON or OFF.
• Light and Camera button : Selects between Monochrome or Color cameras. If using the monochrome camera, allows displaying Pseudocolor.
• Light Path buttons‘: Select between installed light cubes or transmitted light from the condenser.
• Snap to Last ’: Auto-focuses on the last position on the z-axis; used for quick focus on similar types of samples.
• Quick slider “: Calculates the focal range where the sample is located based on the vessel selected.
• Find Sample ” and Auto Fine : Automatic course and fi ne focusing.
• Course and Fine sliders : Used for manually adjusting course and fi ne focus.
• Capture : Captures the current image on the screen.
• Capture All : Captures an image using each of the installed light cubes.
• Z-Stack : Captures a series of images in the z-axis.
• Save : Saves the captured images.

Note:and are Hot Buttons. Hot Buttons are user-specifi ed, programmable buttons that customize the imaging system. Up to four Hot Buttons can be displayed on the Image Capture tab. For more information, refer the EVOS^®FL Auto Imaging System User Guide.

Note: The EVOS^® FL Auto Imaging System is a fully automated imaging system; no manual adjustment is required for the objective turret, focusing controls, light cube and camera selection, etc.