DSI 600-2100-001 Manual
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DSI 600-2100-001 Manual

Plethysmograph chambers
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TM
Life Science Suite
Plethysmograph Chambers
Manual
Model: 600-2100-001, 600-2200-001, 600-2400-001, 600-2500-001, & 600-2600-001
Manual: MU00249-001
Revision 57
Data Sciences International
th
119 14
Street NW, Suite 100
St. Paul, MN 55112
P+(651) 481-7400 F+1(651) 481-7470
http://www.datasci.com

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  • Page 1 Life Science Suite Plethysmograph Chambers Manual Model: 600-2100-001, 600-2200-001, 600-2400-001, 600-2500-001, & 600-2600-001 Manual: MU00249-001 Revision 57 Data Sciences International 119 14 Street NW, Suite 100 St. Paul, MN 55112 P+(651) 481-7400 F+1(651) 481-7470 http://www.datasci.com...
  • Page 2 Copyright© 2012 by Data Sciences International. All rights reserved. No part of this manual may be reproduced, translated, transcribed, or transmitted in any form or by any means manual, electronic, electromagnetic, chemical, or optical without the written permission of Data Sciences International. Data Sciences International 119 14 Street NW, Suite 100...

  • Page 3: Table Of Contents

    Operation     Setup and Operation ........................ 22   DSI Head-Out Chambers Animal Loading Tips ............22   DSI Whole Body Chamber Animal Loading Tips ............. 23   Maintenance and Cleaning ....................... 23 Appendix A     Specifications ........................... 24  ...

  • Page 5: Overview

    Overview Introduction The DSI plethysmograph chambers are designed and built for optimal performance along with the Ponemah Physiological Platform and DSI telemetry systems. Product offerings in this document include the Rat Head-Out Plethysmograph Chamber, the Mouse Head-Out Plethysmograph Chamber, the Guinea Pig Unrestrained Whole Body Plethysmograph Chamber, the Rat Unrestrained Whole Body Plethysmograph Chamber, and the Mouse Unrestrained Whole Body Plethysmograph Chamber.

  • Page 6: Components

    Components Rat Head-Out Plethysmograph Chamber Rat Head-Out Plethysmograph Chamber (600-2100-001) pictured on a telemetry RPC1 receiver plate 2 • Contents Plethysmograph Chamber Manual...
  • Page 7 Rat Head-Out Plethysmograph Chamber components: 1. Rat Head-Out Plethysmography Chamber Accessory (Front) (600- 2110-001) 2. Squeeze plate mechanism (600-2112-001) 3. Rat Head-Out Plethysmography Chamber Accessory (Rear) (600- 2111-001) Contents • 3 Plethysmograph Chamber Manual...
  • Page 8 The Rat Head-Out Plethysmograph Chamber (600-2100-001) is packaged with the following items: 1. Taper Plug 5/16 to ½ OD 2. 1/8-27 NPT to 1/8 barb 3. 1/8-27 NPT to 1/8-27 NPT 4. 1/8-27 NPT Plug 5. 1/8-27 NPT to ¼-28 UNF 6.

  • Page 9: Mouse Head-Out Plethysmograph Chamber

    Mouse Head-Out Plethysmograph Chamber Mouse Head-Out Plethysmograph Chamber (600-2200-001) Mouse Head-Out Plethysmograph Chamber (600-2200-001) components: 1. Body Chamber Contents • 5 Plethysmograph Chamber Manual...
  • Page 10 2. Head Mount Plate 3. Head Plate 4. Screw Pan HD 10-32x7/8 5. Knurled Thumb Nut 6. Female Luer Adaptor 7. Male Luer Plug 8. Male Luer Intergral Lock Ring 9. 1/8 dia x 3/16 screw 10. Stack Airway 11. Screen Retainer Plate 12.
  • Page 11 1. 3 extra Pneumotachograph Screens 2. 2 Fittings, Luer male to 1/8 barb 3. Taper plug 7/16 to 5/8 OD 4. Taper plug 3/16 to 11/32 OD 5. 3 Latex neck seals 6. Tygon tubing 1/8 in. ID (24 in. long) 7.

  • Page 12: Rat Unrestrained Whole Body Plethysmograph Chamber

    Rat and Guinea Pig Unrestrained Whole Body Plethysmograph Chamber 8 • Contents Plethysmograph Chamber Manual...
  • Page 13 Rat and Guinea Pig Unrestrained Whole Body Plethysmograph Chamber (600-2400-001, 600-2600-001) components: 1. Reference Chamber, Top 2. Reference Chamber, Cylinder 3. Reference Chamber, Bottom 4. Reference Chamber, Tube 5. Reference Chamber, Screen Mount 6. Main Chamber, Cylinder 7. Main Chamber, Top Contents •...
  • Page 14 8. O-Ring Silicone Red 9. Latch 10. Body Box Screen Plate 11. Reference Chamber, Screen Plate 12. O-Ring Silicone Red 13. Plate, Air Path 14. Aerosolization port 15. Fitting, Luer Female to 1/8-27NPT 16. Screw Pan HD 4-40x1/4 17. Screw Pan HD 6-32x5/16 18.
  • Page 15 The Rat and Guinea Pig Unrestrained Whole Body Plethysmograph Chambers are packaged with the following items: 1. One water bottle with sipper tube 2. Three luer cap plugs to seal chamber ports 3. One water bottle support bracket 4. Two 1/8” barb luer male fittings to attach tubing to the chamber 5.

  • Page 16: Mouse Unrestrained Whole Body Plethysmograph Chamber

    Mouse Unrestrained Whole Body Plethysmograph Chamber Mouse Unrestrained Whole Body Plethysmograph Chamber (600-2500-001) 12 • Contents Plethysmograph Chamber Manual...
  • Page 17 Mouse Unrestrained Whole Body Plethysmograph Chamber (600-2500-001) components: Contents • 13 Plethysmograph Chamber Manual...
  • Page 18 1. Base Plate 2. Main Cylinder 3. Main Chamber Top 4. Retainer Filter Airway 5. O-Ring Silicone Red 4.193OD 6. Reference Chamber 7. Reference Chamber Top 8. Latch 9. Latch Bracket 10. Airway 11. Luer Hex to UNF 12. Bumper Rubber Black 13.
  • Page 19 The Mouse Unrestrained Whole Body Plethysmograph Chamber is packaged with the following items: 1. One water bottle with sipper tube 2. One ball valve 3. 24 inch length of Tygon tubing (1/8 ID) to plumb the pressure transducer 4. Two 1/8” barb luer male fittings to attach tubing to the chamber 5.
  • Page 20 Rat Unrestrained Whole Plethysmograph Chamber with temperature and humidity sensors (circled in red) Temperature and Humidity Sensors • Interfaces to the 7700 UniversalXE signal conditioner. • Attaches to the lid of the Unrestrained Whole Body Plethysmograph Chamber. • Calibration values marked on the sensors for ease of calibrating the system. •...
  • Page 21 Temperature sensor (left), Humidity sensor (right) with cable. Barometric Pressure Sensor • Interfaces to the 7700 Advanced Basic DC 4 signal conditioner or with the 7700 Advanced Basic DC 32 signal conditioner with the distribution input panel. • Provides a calibrated high level output so that there is no need for a physical calibration. Contents •...

  • Page 22: Accessories

    Dependent on the application, there may be other items that the user requires. Below is a list of optional accessories that work with the Rat Head-Out Plethysmograph Chamber. • Validyne transducer (600-1100-002, 600-1102-002) dependent on usage - contact DSI • Replacement pneumotachograph screens, SS 200x600 (600-1000-001) • Neck seals 600-2114-001 - Polyurethane 1/8 in.
  • Page 23 Dependent on the application, there may be other items that the user requires. Below is a list of optional accessories that work with the Mouse Head-Out Plethysmograph Chamber. • Validyne transducer (600-1100-002, 600-1102-002) dependent on usage - contact DSI • O-Ring Gaskets kit, Qty 3 (600-2212-001) • Neck Seal (latex), Qty 25 (600-2211-01) •...
  • Page 24 Dependent on the application, there may be other items that the user requires. Below is a list of optional accessories that work with the Mouse Unrestrained Whole Body Plethysmograph Chamber. • Validyne transducer (600-1100-002, 600-1102-002) dependent on usage - contact DSI • Temperature Sensor - requires temperature sensor cable for UniversalXE (600-1210-001) •...
  • Page 25 Guinea Pig Unrestrained Whole Body Plethysmograph Chamber. • Validyne transducer (600-1100-002, 600-1102-002) dependent on usage - contact DSI • Temperature Sensor - requires temperature sensor cable for UniversalXE (600-1210-001) •...

  • Page 26: Operation

    NOTE: If using Validyne transducers (or equivalent), ensure the Validyne is secure and is not moved between calibration and measurement, else a baseline shift may result. For usage of DSI chambers please refer to the following Getting Started Guides for detailed information on the usage of the chambers: •...

  • Page 27: Dsi Whole Body Chamber Animal Loading Tips

    • Animals that cannot be easily placed into the chamber entrance should not be forced. See also “Animal Acclimation” located in Appendix A. DSI Whole Body Chamber Animal Loading Tips • Remove the top of the chamber. • Gently place the animal inside the chamber.

  • Page 28: Specifications

    Appendix A Specifications Rat Head-Out Chamber Dimensions Length 12.5 inches (31.8 cm) Width 4 inches (10.2 cm) Height 4 inches (10.2 cm) Volume 76 cubic inches (1,245.42 cubic cm) Weight 2.4 lbs (1.1 kg) Mouse Head-Out Chamber Dimensions Length 10 inches (25.4 cm) Width 3 inches (7.6 cm) Height...

  • Page 29: Troubleshooting

    Mouse Unrestrained Whole Body Chamber Overall Dimensions Length 9 inches (22.9 cm) Width 5 inches (12.7 cm) Height 6.75 inches (17.1 cm) Weight 2.9 lbs (1.34 kg) Animal Chamber Dimensions Width 4.375 inches (11.1 cm) Height 3.75 inches (9.5 cm) Volume 54 cubic inches (924 cubic cm) Guinea Pig Unrestrained Whole Body Chamber...

  • Page 30: Compromised Neck Seal

    Guinea Pig Unrestrained Whole Body Chamber • Pneumotachograph Screens (600-1001-001) for use with: Mouse Head-Out Chamber Compromised Neck Seal If gains are being set extremely high where noise and temp drift are causing major shifts in the signal, the neck seal may have become compromised. Also, if the signal volume drops out randomly, the neck seal may have become compromised.

  • Page 31: Animal Acclimation

    Animal Acclimation In order to obtain the best quality data from the Head-Out Chambers it is recommended that the test subjects undergo acclimation training. The following is a for acclimation training: • After receiving the animals and following 1 week general acclimation test subjects are allowed to explore the chamber without any restrain.

  • Page 32: Product Issue Report

    Product Issue Report Product Issue Report Form Sales Person: Issue: Customer Name: Company: Address: Phone Number: Email Address: P3 Plus Version (including Service Pack): Serial Number: Priority: Date: Hardware: Steps to Repeat Status of issue (check one) [] Unreproduced [] Reproduced [] Needs repair [] As intended Computer hardware/software...

  • Page 33: Feature Request

    Feature Request Feature Request Form Sales Person: Description: Customer Name: Company: Address: Phone Number: Email Address: P3 Plus Version (including Service Pack): Serial Number: Priority: Date: Hardware: Feature (check one) [] Unevaluated [] Pending [] Implement [] Already Exists Contents • 29 Plethysmograph Chamber Manual...

This manual is also suitable for:

600-2200-001600-2400-001600-2500-001600-2600-001

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