WP EVOM EVA-MT-02-01 Instruction Manual

Auto automated teer measurement system

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INSTRUCTION MANUAL

EVA-MT-02-01

EVOM™ Auto Automated TEER Measurement System

Serial No._____________________

WORLD

PRECISION

INSTRUMENTS

www.wpiinc.com

030923

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Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL EVA-MT-02-01 EVOM™ Auto Automated TEER Measurement System Serial No._____________________ www.wpiinc.com 030923...
Page 3: Table Of Contents
EVA-MT-02-01 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Features............................2 Notes and Warnings: ........................ 3 Parts List ............................3 Unpacking ........................... 3 INSTRUMENT DESCRIPTION ......................4 EVOM™ Auto System ........................ 4 Autosampler ..........................5 Interface Unit ..........................6 Software ............................
Page 4 WARRANTY .............................35 Claims and Returns ........................35 Repairs ............................35 World Precision Instruments...
Page 5: About This Manual
EVA-MT-02-01 ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat.
Page 6: Introduction
INTRODUCTION The EVOM™ Auto automates TEER measurements. The instrument is controlled through a web browser, and the measurements are stored in the instrument and downloaded to your computer through the web browser. At present, the instrument supports MatTek , Corning, and Millipore 96 well plates. EVOM™ Auto is controlled wirelessly via Wi-Fi connectivity using an iPad (included with the system) or any other device with Wi-Fi connectivity.
Page 7: Notes And Warnings
EVA-MT-02-01 Notes and Warnings: CAUTION: Never set anything (especially liquids) on top of the autosampler. Parts List After unpacking, verify that there is no visible damage to the equipment. Verify that all items are included: (1) Autosampler* with plate alignment option for MatTek, Corning or Millipore plates (1) 96 HTS Electrode Array (1) iPad Generation 9 Control Tablet (1) Interface Unit...
Page 8: Instrument Description
INSTRUMENT DESCRIPTION EVOM™ Auto System Fig. 2—The EVOM™ Auto System, includes the iPad, the autosampler, the Interface Unit and the Power Supply (not shown). The EVOM™ Auto consists of these major components: • Autosampler – The robot contains the electrodes, a 3-compartment rinse stations, and a well plate area.
Page 9: Autosampler
EVA-MT-02-01 Autosampler Fig. 3—The autosampler takes the TEER measurements of the HTS well plate. Here are the primary components of the autosampler. Electrode Array Head – The electrode array rests in the Home position (shown in Fig. 3), just to the right of the rinse stations. The LED on the electrode array will: •...
Page 10: Interface Unit
Looking at the autosampler measurement plate from the top, you can see the: • 4 Base Positioning Knobs used when you need to reposition the well plate when you are changing plate manufacturers • Rinse Station Pocket where the rinse station slides into place •...
Page 11: Software
EVA-MT-02-01 Software The EVOM™ Auto software runs in a web browser, and is not tied to any particular operating system or device. An pre-configured iPad is provided with the system. Fig. 7—The Main screen gives you access to all the main menu items and an overview of the selected plate.
Page 12: Emergency Stop
Emergency Stop On any window where the electrode array can move, you can access the Emergency Stop button. It is the red stop icon located in the bottom right corner of the window. Fig. 8—The emergency stop button is located in the bottom right corner of any window where the electrode array can move.
Page 13: Setup
EVA-MT-02-01 SETUP Refer to the Install Guide for the hardware setup instructions. Once all the components are properly connected, press the Power button on the interface unit to turn on the system. The blue ring around the button illuminates. Allow the system to boot up. On the iPad, navigate to the Setting area and select WiFi.
Page 14 Fig. 13—Home screen. World Precision Instruments...
Page 15: Operating Instructions
EVA-MT-02-01 OPERATING INSTRUCTIONS After your system is set up and configured, you may use the browser on the iPad to control the autosampler. It is important to note that the Plate Name ties the elements together in the EVOM™ Auto software. The measurement options and date are attached to the Plate Name.
Page 16: Selecting A Plate Brand
Selecting a Plate Brand The electrode array is configured to support 96-well plates. Three different brands of plates are supported: • MT96_HTC – MatTek 96 Well Plate • C96_HTC – Corning 96 Well Plate • M96_HTC – Millipore 96 Well Plate To select the desired brand, click on the brand name under the EVOM™...
Page 17: Creating A Plate File And Setting Up Measurements
EVA-MT-02-01 Creating a Plate File and Setting up Measurements When the system starts, the Home window displays. Fig. 16—The Main Window appears. To create a measurement sequence, select the Manage Sequence menu. A list of the existing sequences displays (Fig. 17), and you may open and edit any existing sequence or select New Sequence to create a new one.
Page 18 Fig. 19—The Sequence window lets you define a sequence. You may choose to display the measured resistance values or the TEER measurement. TEER is a calculated value based on the surface area of the well and the measured resistance. Select the Mode menu. Options include Resistance and TEER.
Page 19 EVA-MT-02-01 Fig. 21—The values of the blanks are measured to determine the baseline resistance without any cells. To start the blank setup, select the Blank menu and choose Load Blank, and then New Blank (Fig. 22) to create a blank baseline data file. A popup asks for a name for your new blank file or data set (Fig.
Page 20 the EVOM™ Auto is about to measure the resistance values of the first column and that a well plate with the desired level of buffer or media solution must be placed in the well plate area to be measured (Fig. 24). Press Continue to begin or Cancel to abort the action.
Page 21 EVA-MT-02-01 11. The three rinse stations may be used for rinsing, disinfecting, stabilizing or any other function desired. Configure the rinse schedule by selecting Rinse from the menu. The Rinse Time window appears (Fig. 27). You may set the rinse time and the wait time in each rinse station using the toggle controls on each field.
Page 22: Running An Experiment
LAST COLUMN MEASURED TRAVEL TO COLUMN 1 LOWER ARRAY INTO COLUMN MEASURE WELLS TRAVEL TO RINSE STATION 2 TRAVEL TO RINSE STATION 2 LOWER ARRAY INTO RINSE STATION LOWER ARRAY INTO RINSE STATION WAIT 2 SECONDS WAIT 2 SECONDS LIFT ARRAY LIFT ARRAY WAIT 3 SECONDS WAIT 3 SECONDS...
Page 23 EVA-MT-02-01 • Details area lets you set your plate name, choose a sequence, select your blank and make notes. • Plate Controls/Schedule Controls areas have all the buttons for controlling an experiment or scheduling an experiment. • System Controls area has the buttons to manage the data files and the emergency shut off for the autosampler.
Page 24 Set Cycles – Brings up a popup to set the number of scans to be performed according to the current sequence. Set Period – Brings up a popup to set the length of time in between well plate scans. In the Plate Controls area, available options are shown in color. If an option is not available, the button will be grey.
Page 25 EVA-MT-02-01 To begin running an experiment: Select Experiment from the main menu. The Experiment window appears (Fig. 35). Fig. 35—The Experiment window appears. Select the Plate Name drop-down in the Details area on the right side of the screen. Choose from the list of existing plates or select New Plate to define a new plate.
Page 26 Fig. 37—Confirmation window. Select the Sequence Name drop-down in the Details area on the right side of the screen. A list of existing sequences appears. Select the appropriate sequence that you created earlier. A blank data table with no data values displays in the window. At this point, select which wells you want to measure.
Page 27: Downloading The Data File
EVA-MT-02-01 At any time, you may add comments for the data run being collected. Click inside the Notes text block in the Details section on the right side of the screen to attach notes to the plate run. TIP: You may select the wells containing samples. Reading empty wells takes longer to finish reading the sample plate.
Page 28: Deleting A Data File
For each run there is a header section. The first run at the top of the file is the most recent. A sample file is shown in Fig. 41. Run Number Date of Data Acquisition Comments Sequence Parameters Fig. 41—Sample data file. Following the header is the data table.
Page 29: Transferring Files Between Ipad And Computer
EVA-MT-02-01 Transferring Files Between iPad and Computer There are multiple ways to move files on your iPad to your Mac or Windows PC, and vice versa. • Wirelessly (email, messages, or AirDrop) – Refer to the iPad online User Guide topic Transfer files wirelessly between your iPad and computer with email, messages, or...
Page 30: Scheduling Operations
Scheduling Operations The data acquisition functions of the EVOM™ Auto may be repeated at a defined time interval when multiple scans of a plate are needed. The scheduled operations are displayed on the right side of the Experiment window in the Current State area (Fig. 44). The control buttons for scheduling are in the Schedule Controls area on the right side of the window (Fig.
Page 31 EVA-MT-02-01 Fig. 48—(Left) If you select the pencil icon, you may use the keyboard to enter a four-digit number in the form of HHMM to set the period. Fig. 49—(Right) If you used the spinner to set the hours of the period, then the display changes to allow you to set the minutes of the period.
Page 32: Stopping A Running Scheduled Sequence
Stopping a Running Scheduled Sequence Press the Stop button in the Schedule Controls area to stop the schedule. The current scan will continue until it completes the cycle. The Stop and Pause buttons become active in the Plate Controls area. If you need to stop the scan, you must also press the Plate Controls Stop button.
Page 33: Maintenance
EVA-MT-02-01 MAINTENANCE The electrodes require regular maintenance to keep them performing optimally. Chloridizing – To replenish the chloride on the surface of the electrodes, the electrode tips need to be submerged in a bleach solution for at least 10 minutes, followed by a rinse with distilled water.
Page 34: Chloriding The Electrode Array
Chloriding the Electrode Array Select Maintenance on the main menu. The Maintenance window appears. To chloridize, press the + or – buttons to set the timer to the desired time in minutes in the Chloridize frame. Select the radio button for the rinse station containing the chloridizing solution. Fill the appropriate rinse station with a bleach solution.
Page 35: Accessories
EVA-MT-02-01 ACCESSORIES Part Number Description EVM-EL-xxxx EVOM™ Auto Electrode Array CCI96-PET-0.4 PermaCell Cell Culture Insert Plates, 96-Well, PET, 0.4µm, pkg of 1 CCI96-PET-0.4-5 PermaCell Cell Culture Insert Plates, 96-Well, PET, 0.4µm, pkg of 5 TROUBLESHOOTING Issue Possible Cause Solution You may not have adequate api- Increase the liquid volumes.
Page 36: Specifications
SPECIFICATIONS This unit conforms to the following specifications: Autosampler Dimensions (W×D×H) ................16×10×8.4” Autosampler Weight ......................15.5 lbs. Interface Unit Weight ......................... 1 lb. CE Certified .............................Yes Compatibility .................Wide variety of 96-well HTS Plates Resistance Range ..................10KΩ, 50KΩ, 100KΩ Number of Rinse Stations ......................3 Electrode Array for 96 HTS Plate ......
Page 37: Index
EVA-MT-02-01 INDEX Symbols run scheduled 19 –––– 7 instrument description 4 interface unit 4, 6 save 20 interface unit connection Autosampler 4, 5, 8 schedule 18, 19, 26 port 5 sequence name 22 set cycle 27 back 20 maintenance 29 set cycles 20 bad measurements 7 MatTek 12 set period 20, 26 baseline 14...
Page 38: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments...
Page 39 EVA-MT-02-01 WARRANTY WPI (World Precision Instruments) warrants to the original purchaser that this equipment, including its components and parts, shall be free from defects in material and workmanship for a period of one year* from the date of receipt. WPI’s obligation under this warranty shall be limited to repair or replacement, at WPI’s option, of the equipment or defective components or parts upon receipt thereof f.o.b.
Page 40 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...