High Meniscus Liquids; Agglutination Method - tecan SUNRISE Operating Manual

6.3.3

High Meniscus Liquids

Me a surem ent Po sitio ns

Agglutination Method

R
100%
50%
0%
1
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7
When measuring liquids that produce a high meniscus, the measured optical
density can be incorrect as the instrument normally measures the optical density
at three positions in the well and uses this average measured optical density as
the optical density for the well.
Normal mode
Liq uid with
norma l m enisc us
Me a surem ent Po sitio ns
for Fa st a nd Ac c ura te
for Fa st a nd Ac c ura te
Mo d es
Open SUNRISE Instrument Setting software to set the SPECTRA Mode. For
agglutination measurements an external software package such as Software
2000 must be used.
Instruments with option tuneable wavelength selection are not able to
perform agglutination measurements.
After the microplate has been measured, select a well and zoom it so that it is
printed in large scale.
Normal Liquid
C C
10 20 30
Measurement
If the printout has less than sixteen measurement points in the middle of the well,
that are at the same level, use the center measurement.
6. Performance Testing / Quality Control
Liq uid with Liq uid with
hig h m enisc us norma l m enisc us
Me a surem ent Po sitio n
for Ce ntre Mo d e
Mo d es
Liquid with High Meniscus
R R
100%
100%
50%
50%
0%
0%
40 1
Center Mode
Liq uid with
hig h m enisc us
Me a surem ent Po sitio n
for Ce ntre Mo d e
C C R
10 20 30 40
Measurement
100%
50%
0%
6-5