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Summary of Contents for Lambda OMNICOLL
- Page 1 Operation MANUAL LAMBDA OMNICOLL Fraction Collector - Sampler LAMBDA OMNICOLL Fraction Collector and Sampler...
- Page 2 LAMBDA OMNICOLL Fraction Collector - Sampler The LAMBDA OMNICOLL introduces new concepts in fraction collection and sampling. The OMNICOLL fraction collector and sampler can be used for all kinds of chromatography. In the number of simultaneous chromatographic streams, the OMNICOLL is not outperformed by any other fraction collector on the market.
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Page 3: Table Of Contents
Programming of the fraction collector Principle of programming................... 14 Ready to use Fraction collector with the supplied tube racks ........15 Programming the OMNICOLL for any tube racks or recipients ........ 16 3.3.1 Coding X-axis for tube distance in row 3.3.2... -
Page 4: Setting Up The Fraction Collector
Operation MANUAL 2023.01 1 SETTING UP THE FRACTION COLLECTOR 1.1 Assembling the fraction collector The setup of the fraction collection - sampler, LAMBDA OMNICOLL is very easy - a short video of the installation can be found at www.youtube.com/watch?v=33J9U_2-b-o Figure 1.1-1 Push the first tub-sheet into the Figure 1.1-2 Push the second tub-sheet into the... - Page 5 LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 Figure 1.1-7 Push the metal rod through both Figure 1.1-8 Place the fixing mat in the support. fixation nuts on the right side of the frame and When needed clean the mat from dust and dirt fasten them with screws.
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Page 6: Assembly Of Fraction Collection Tubing (Single Stream)
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 1.2 Assembly of fraction collection tubing (single stream) (a) PTFE tubing (external diameter 1.8 mm) (b) Fixing nut (c) O-ring (1 x 1.5 mm) (d) Tubing guide Figure 1.2-1 Place the magnetic tubing holder Figure 1.2-2 Push the delivered PTFE tubing through... -
Page 7: How To Increase The Capacity Of The Fraction Collector
12 streams and 250 ml fraction collection bottles. The tube capacity of the LAMBDA OMNICOLL fraction collector and sampler can be easily increased by the addition of lower support. Virtually any number of capacity extensions can be added. -
Page 8: Multi-Channel Fraction Collection - Multiple Stream Sampling
Operation MANUAL 2023.01 1.4 Multi-channel fraction collection – multiple stream sampling An interesting property of the LAMBDA OMNICOLL fraction collector is the option to collect simultaneously the streams of many chromatographic columns. For LAMBDA OMNICOLL multi-stream fraction collectors, different designs exist: •... - Page 9 LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 Figure 1.4.1-6 Insert the rectangular bar into the Figure 1.4.1-7 Slide the right end of the bar bar holder as shown. through the opening on the guide plate. Figure 1.4.1-9 Slide the tubing guide into the Figure 1.4.1-8 Screw the bar holder on the...
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Page 10: Multi-Stream Fraction Collection Version 2022
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 1.4.2 Multi-stream fraction collection VERSION 2022 LAMBDA OMNICOLL with accessories for multi-stream fraction collector VERSION 2022 can be carried out from 2 to 64 simultaneous threads. LAMBDA OMNICOLL accessories for multi-stream fraction collection VERSION 2022 are: •... -
Page 11: Mounting #6950: Mobile Multi-Stream Collection Adjustable Up To 32 Channels
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 1.4.2.1 Mounting #6950: mobile multi-stream collection adjustable up to 32 channels Figure 1.4.2-5 First you need to assemble the Figure 1.4.2-6 The holder is fixed with two moving arm holder. screws using a hexagonal key. - Page 12 LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 Figure 1.4.2-11 The tube guide is mounted on Figure 1.4.2-12 The tube guide is fixed with a moving arm in accordance with the selected one bolt. position. (For each stream one tube guide (art. no. 6950) is needed).
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Page 13: Connecting The Drop-Counter Detector (Optional)
Let the drop-counter cable (F) pass together with the PTFE tubing (D) through the magnetic holder (H). Figure 1.5-5 Connect the OMNICOLL fraction Figure 1.5-6 If required, the LAMBDA pump or collector to the “OMNICOLL” socket in the valve to the “PUMP/VALVE” socket in the communication module. -
Page 14: Start Of The Fraction Collection
Be sure that nothing will disturb the movement of the control unit during fraction collection. ✔ Connect the cable of the control unit of the OMNICOLL fraction collector and sampler with the power supply plug. -
Page 15: Programming Of The Fraction Collector
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 3 PROGRAMMING OF THE FRACTION COLLECTOR A short video on programming the OMNICOLL fraction collector can be found at www.lambda-instruments.com/fraction-collector/ - video 3.1 Principle of programming Laboratory practice shows that programming of instruments equipped with microprocessors is not simple and mistakes are easily made, especially when such instruments are used occasionally. -
Page 16: Ready To Use Fraction Collector With The Supplied Tube Racks
This simple coding principle allows the use of any racks and fraction stands or other recipients with the LAMBDA OMNICOLL fraction collector and sampler. 3.2 Ready to use Fraction collector with the supplied tube racks The magnetic coding bands programmed with white lines for the desired tube racks are provided with the OMNICOLL Fraction collector. -
Page 17: Programming The Omnicoll For Any Tube Racks Or Recipients
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 3.3 Programming the OMNICOLL for any tube racks or recipients 3.3.1 Coding X-axis for tube distance in row Measure the distance between the tube centres of the desired rack and choose the number of fractions in a row. -
Page 18: Coding Y-Axis For Row Distance
Otherwise the control unit will stop at the position of the next row. The first row line of the Y-axis should be ca 10 cm from the front. 3.4 Control Panel of OMNICOLL Fraction Collector www.lambda-instruments.com... -
Page 19: Selection Of Linear, Meander-Like Or Row Collection
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 3.4.1 Selection of linear, meander-like or row collection The LAMBDA OMNICOLL fraction collector-sampler allows the following operation modes: Line: The fractions are collected from the left to the right. After the last fraction of the row, the collecting arm moves to the left to the first position of the next row. -
Page 20: Time Resolution (0.1 Or 1 Minute)
(about 2 mm), this difference in position is of approx. 1 mm 3.4.2 Time resolution (0.1 or 1 minute) The time resolution of the OMNICOLL fraction collector can be selected in the following way: Figure 3.4-6 Press the button COUNT/TIME Figure 3.4-7 The actual time resolution will appear on the display “0.1M”... -
Page 21: Drops And Count Divider (Division Co-Efficient)
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 3.4.3 Drops and count divider (Division co-efficient) This function allows increasing considerably (by 60 times) the volume of fractions either using a drop counter (art. no. 6926) or motor impulsions counter (counts). -
Page 22: Address Selection (For Pc Control)
Operation MANUAL 2023.01 3.4.4 Address selection (for PC control) When the OMNICOLL fraction collector and sampler has been equipped with the optional RS-232 interface, it can be controlled digitally, e.g., from a PC. Figure 3.4-15 The message “A” and two Figure 3.4-14 While keeping the direction... -
Page 23: Calibration Of The Peristaltic Pump And The Omnicoll
3.6 Calibration of the peristaltic pump and the OMNICOLL The LAMBDA peristaltic pump is connected to the OMNICOLL fraction collector via the remote-control cable (Art. No. 4810-s) and the communication module box (Art. No. 6911 or Art. No. 6929). -
Page 24: Fraction Collection With A Time Interval In-Between Fractions ("High")
3.7 Fraction collection with a time interval in-between fractions (“high”) LAMBDA OMNICOLL fraction collector and sampler could be used for the collection of samples with a time interval between consecutive fractions varying between 0.1 min and 16.6 hours (999.9 min) or 1 min and 166 hours (9999 min). - Page 25 The collector will take the number of samples as programmed under the NUMBER setting and will stop for the time programmed under the PAUSE setting. This cycle will go on until a STOP signal will be encountered. The LAMBDA peristaltic pump is activated automatically only during fraction collection.
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Page 26: Remote Controls
The fraction collector can take one or several samples after detection of a signal 3 to 12 V (or 12 to 30V with a 3300 ohm resistor switched in series). The LAMBDA peristaltic pump will be activated automatically. -
Page 27: Practical Advice
7 FOR YOUR SAFETY Thanks to the use of a plug-in power supply giving only a low voltage of 9 V DC the danger of electrical shock during the use of the LAMBDA OMNICOLL fraction collector and sampler has been virtually eliminated. -
Page 28: Technical Specifications
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 8 TECHNICAL SPECIFICATIONS 8.1 General specification LAMBDA OMNICOLL – microprocessor-controlled Type: programmable fraction collector – sampler Collection mode: Linear (line), meander (zigzag) or row collection Normal modus: Time: 0.1 to 999.9 minutes (16.67 hours) in 0.1 min steps or 1 to 9999 minutes (166.7 hours) in 1 min steps... -
Page 29: Inputs/Outputs
8.2 Inputs/outputs Colour Description blue Input remote speed control +3-12V green Impulses of the stepping motor of the LAMBDA peristaltic pump (0 and 12 V) white Reserved for RS-232 TTTL Input voltage + 9 V brown Output remote control for the pump (+ 9-12 V) - Page 30 LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 4810-ISM15 Remote control cable for ISMATEC multi-channel pump (15 pin connector) 4810-ISM15-9 Converter cable for ISMATEC multi-channel pump (15 pin to 9 pin connector) 4810-ISM25 Remote control cable for ISMATEC/Masterflex REGLO Digital multi-...
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Page 31: Guarantee
LAMBDA. ● The customer will send the instrument to our service office. ● Damage or loss of items during transport will not be compensated for by LAMBDA. ● Failure to fulfil these requirements will disqualify the customer from compensation. -
Page 32: Appendix
LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01 11 APPENDIX 11.1 RS communication protocol for LAMBDA OMNICOLL fraction collector-sampler 11.1.1 Format of data sent by the PC to the fraction collector and back Data sent by the PC: #ss mm a xxxx qs c Data sent back by the fraction collector: <mm ss a xxxx qs c... -
Page 33: Checksum Control
1: pulse setting (COUNT) 2: pause time setting (PAUSE) 3: number of fractions setting (NUMBER) The answer of the OMNICOLL fraction collector-sampler is the following: < mm ss B xxx.x qs c time xxx.x in 0.1 minute steps (collector is in stand-by mode) <... -
Page 34: Format Of The Data Transmission
8 data bits, odd parity, 1 stop bit 11.2 How to set the OMNICOLL Fraction Collector-Sampler address? When the LAMBDA OMNICOLL fraction collector and sampler has been equipped with the optional RS-232 interface, it can be controlled digitally, e.g., from a PC.
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