Related Manuals for SCIEX Anion Analysis Kit
Summary of Contents for SCIEX Anion Analysis Kit
- Page 1 SCIEX Anion Analysis Kit For P/ACE™ MDQ and P/ACE™ MDQ plus Capillary Electrophoresis Systems Instruction Guide A49108AE May 2015...
- Page 2 In no event shall AB Sciex Pte. Ltd. and its affiliates be liable, whether in contract, tort, warranty, or under any statute or on any other basis for special, incidental, indirect, punitive, multiple or consequential damages in connection with or arising from this document, including but not limited to the use thereof.
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Page 3: Table Of Contents
Chapter 1 Using the Anion Analysis Kit ....... . 5... - Page 4 Contents SCIEX Anion Analysis Kit Instruction Guide 4 of 22 A49108AE...
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Page 5: Chapter 1 Using The Anion Analysis Kit
Using the Anion Analysis Kit Introduction The SCIEX Anion Analysis Kit contains the supplies necessary for the separation and quantitation of anions, using the SCIEX P/ACE MDQ and P/ACE MDQ plus Capillary Electrophoresis Systems. Each anion kit yields approximately 500 tests. -
Page 6: Materials And Reagents
Using the Anion Analysis Kit Materials and Reagents Table 1-1 Kit Contents (PN A53537) Component Quantity Anion Coating Anion Separation Buffer Conditioner — Na Anion Acid Rinse Anion Internal Standard Anion Organic Test Mix Anion Inorganic Test Mix Capillary, 50 cm, 75 µm I.D. -
Page 7: Cleaning Vial Caps
Using the Anion Analysis Kit Cleaning Vial Caps Note: The vial caps may contain impurities that can be detected with the Anion Analysis Kit, therefore wash the caps before use. 1. Using a clean beaker, rinse the vial caps twice with double-deionized (DDI) water. -
Page 8: Conditioning A New Capillary
Using the Anion Analysis Kit Conditioning a New Capillary After installing a new capillary, rinse the capillary for one minute with Conditioner — Na. Wait four minutes, then rinse for 30 seconds with Conditioner — Na. Rinse for one minute with Rinse Solution. -
Page 9: Preparing The Sample
Using the Anion Analysis Kit Figure 1.1 Buffer Tray Configuration for Anion Analysis 4. Load the Inlet Buffer and Outlet Buffer trays in the instrument. Note: When analyzing fluoride and/or phosphate ions, fill a vial with Anion Acid Rinse and place it in position F1 of the Inlet Buffer Tray. The use of the Anion Acid Rinse is required when running the Anion Inorganic Test Mix to avoid peak tailing. -
Page 10: Pcr Vial Setup-P/Ace Mdq System
Using the Anion Analysis Kit PCR Vial Setup–P/ACE MDQ System Fill a PCR vial with 200 µL of test or sample mix. Make sure there are no air bubbles at the bottom of the PCR vial. Air bubbles can affect the sample injection. If bubbles exist, centrifuge the vials for 2 minutes at 1000 x g and repeat if necessary. -
Page 11: Universal Vial Setup-P/Ace Mdq Plus System
Using the Anion Analysis Kit Universal Vial Setup–P/ACE MDQ plus System Fill a PCR vial or micro vial with 200 µL of test or sample mix. Make sure there are no air bubbles at the bottom of the vial. Air bubbles can affect the sample injection.If bubbles exist, centrifuge the vials for 2 minutes at 1000 x g and repeat if necessary. -
Page 12: Initial Conditions For All Methods
Using the Anion Analysis Kit Initial Conditions for All Methods All three methods utilize the same Initial Conditions (Figure 1.4) and UV Detector Settings (Figure 1.5). Note: When analyzing samples containing inorganic anions or organic acids that absorb in the low UV (such as nitrate and azide), the detection wavelength (Figure 1.5) -
Page 13: Time Programs For Anion Separation Methods
Using the Anion Analysis Kit Figure 1.6 Time Program for Anion Capillary Conditioning Method Time Programs for Anion Separation Methods There are two Anion Separation methods: one for the P/ACE MDQ system and the other for the P/ACE MDQ plus system. The difference between the two methods is the duration of the Anion Coating rinse (step 1 in the Time Program): •... -
Page 14: Time Program For Anion Shutdown Method
Using the Anion Analysis Kit Time Program for Anion Shutdown Method Figure 1.9 Time Program for Anion Shutdown Method Checking System Performance Using the Anion Organic Test Mix To check system performance, run the Anion Organic Test Mix after performing the Capillary Conditioning method. -
Page 15: Using The Anion Inorganic Test Mix
Using the Anion Analysis Kit Using the Anion Inorganic Test Mix To check system performance, run the Anion Inorganic Test Mix after performing the Capillary Conditioning method. Compare the electropherogram obtained with the one shown in Figure 1.11. The electrical current during the separation should be stable around -53 µA. A negative electrical value indicates that reverse polarity was used in the separation. -
Page 16: Troubleshooting
Using the Anion Analysis Kit The parameters have the following effects on the integration: • Integration off sets time intervals in the electropherogram that are not integrated. • Width sets the sensitivity of the peak detection regarding changes in the baseline. -
Page 17: Appendix A Filter Installation
14. Turn on the instrument. 15. Follow the instructions in Configuring the P/ACE™ MDQ or P/ACE™ MDQ plus System on page 19 to configure the 32 Karat software for performing anion analysis. Instruction Guide SCIEX Anion Analysis Kit A49108AE 17 of 22... - Page 18 Filter Installation SCIEX Anion Analysis Kit Instruction Guide 18 of 22 A49108AE...
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Page 19: Appendix B System Configuration
9. Click the Green arrow. The UV detector icon should now be on the right side under Configured Modules. Figure B.1 P/ACE MDQ CE Configuration for Anion Analysis 10. Double-click the UV Detector icon to display the configuration settings. Instruction Guide SCIEX Anion Analysis Kit A49108AE 19 of 22... - Page 20 For the P/ACE MDQ plus system–refer to Figure B.3. Figure B.2 Anion Analysis Settings–P/ACE MDQ System Figure B.3 Anion Analysis Settings–P/ACE MDQ plus System 11. Click OK to accept the detector configuration. SCIEX Anion Analysis Kit Instruction Guide 20 of 22 A49108AE...
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Page 21: Activating Caesar Integration
1. In the CE Configuration dialog, click Options (Figure B.4) Figure B.4 P/ACE MDQ CE Configuration for Anion Analysis 2. Under General, make sure that only Qualitative Analysis and Caesar Integration are selected (Figure B.5). Instruction Guide SCIEX Anion Analysis Kit A49108AE 21 of 22... - Page 22 System Configuration Figure B.5 Configuration Options Dialog 3. Click OK in the next three windows to accept the changes. SCIEX Anion Analysis Kit Instruction Guide 22 of 22 A49108AE...
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