Related Manuals for biosystems X-CLARITY
Summary of Contents for biosystems X-CLARITY
- Page 1 X-CLARITY ™ Tissue Clearing System User Manual www.logosbio.com LBSM-0005 V160726-01...
- Page 2 The contents of this document may be changed in the future without advance notice. All copyrights of this manual are owned by Logos Biosystems. The X-CLARITY™ Tissue Clearing System is a set of laboratory electrical instruments for scientific research use only. It is not a medical, therapeutic, or in vitro diagnostics device.
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Page 3: Table Of Contents
Safety Information ..................................... 3 Environmental Conditions for Operation ..............................3 Introduction ..................................... 4 Product Overview .................................. 4 Product Contents ................................... 4 Product Specifications ................................5 ™ 1.3.1 X-CLARITY ETC Chamber ............................5 ™ 1.3.2 X-CLARITY ETC Controller ............................5 1.3.3 X-CLARITY ™... -
Page 4: Safety Information
Avoid electric shock while operating the instruments. Do not touch the components with wet hands. Operate the instrument in the conditions described in the Environmental Conditions for Operation. Wear proper personal protective equipment (PPE) when handling Electrophoretic Tissue Clearing Solution or X-CLARITY ™... -
Page 5: Introduction
Lipid bilayers are broken up through electrophoresis in the presence of ionic detergents, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping. Logos Biosystems licensed and independently developed the CLARITY technology to make significant improvements for tissue ™ clearing efficiency and reproducibility. The X-CLARITY Tissue Clearing System is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. -
Page 6: Product Specifications
Internal Dimensions (W x D x H) 57 mm x 30 mm x 93 mm Weight 2.8 kg Note: Power to the X-CLARITY ™ ETC Chamber is supplied by the X-CLARITY ™ ETC Controller via the multi-cable. 1.3.2 X-CLARITY ETC Controller ™... -
Page 7: X-Clarity
An integrated cooling system dissipates the heat generated during electrophoresis. A magnetic safety switch detects the opening and closing of the safety cover. If the cover is open, the X-CLARITY™ ETC Controller cannot supply power to the ETC Chamber. -
Page 8: X-Clarity ™ Etc Controller
ETC Controller ™ The X-CLARITY ™ ETC Controller controls the power, temperature, and safety for the X-CLARITY ™ ETC Chamber through one cable, eliminating the need for dangerous and messy wiring. To adjust values for the current, temperature, or timer, push the SET button located on the front panel of the ETC Controller. -
Page 9: X-Clarity ™ Pump
2.3 X-CLARITY Pump ™ The peristaltic pump circulates the buffer to maintain consistent buffering capacity and temperature. Always use the pump to fill or drain the ETC Chamber. Do not pour liquids in directly. Depending on how the pump has been oriented in relation to the ETC Chamber, run the pump in a clockwise (CW) or ▶▶... -
Page 10: Installation
Make sure the tube is between the rollers and the compression block. Release the clamps. Pull the pump head lever to the right to close the compression block. The provided peristaltic pump tube has a tubing life of 3,000 hours. Only use tubes provided or approved by Logos Biosystems. -
Page 11: Snap-Lock Connector Tube: Etc Chamber Outlet And The Reservoir Inlet
3.4 Snap-Lock Connector Tube: ETC Chamber Outlet and the Reservoir Inlet Screw the chamber cap onto the ETC Chamber. Close the safety cover. Attach the plastic head of the snap-lock connector tube to the chamber outlet protruding from the chamber cap. You will feel and hear a click when it has attached properly. -
Page 12: Running
4 Running 4.1 Buffer Flow Fill the reservoir with 1.2 L Electrophoretic Tissue Clearing Solution. Use fresh Electrophoretic Tissue Clearing Solution with each run. Reused clearing solution may have lowered buffering capacity, which reduces clearing efficiency. Prolonged use of Electrophoretic Tissue Clearing Solution may cause electrolysis of the extracted lipids, turning the tissue yellow. Screw on the reservoir cap. -
Page 13: Tissue Clearing
1.5 A after a few hours, check the Electrophoretic Tissue Clearing Solution. The tissue container holder design can also affect the resistance, so use the container holder provided by Logos Biosystems. As the system runs, you will see bubbles formed by water electrolysis coming from the outlet. A significant amount of heat will be generated in the ETC Chamber during the tissue clearing process. -
Page 14: Chamber Opening
4.4 Chamber Opening Push the RUN/STOP button on the ETC Controller to stop clearing. Turn the ETC Controller off. Reverse the direction of the pump (CW to CCW or CCW to CW) to drain the ETC Chamber and the snap-lock connector tube. The snap-lock connector tube will empty into the ETC Chamber. -
Page 15: Maintenance And Troubleshooting
5 Maintenance and Troubleshooting 5.1 ETC Chamber Cleaning Wash the ETC Chamber interior with fresh Electrophoretic Tissue Clearing Solution followed by deionized water after each run. Using the pump, fill the ETC Chamber with 500 mL fresh Electrophoretic Tissue Clearing Solution. Use the following settings: Recommended Current... -
Page 16: Troubleshooting
It is not unusual for the displayed Contact your local distributor or Logos temperature are temperature to go above the set value. Biosystems when the difference is ≥10°C. different Convection is a normal phenomenon. The High temperatures in the temperature of the clearing solution in the... -
Page 17: Error Codes
Possible Cause Solution Reboot your system. If the error code persists, contact your local Malfunctioning part distributor or Logos Biosystems. Overheating due to high environmental Set up your system in a cooler environmental temperature (less temperature, a malfunctioning controller, than 35°C). If the error code persists, contact your local distributor or a malfunctioning cooling system or Logos Biosystems. -
Page 18: Ordering Information
Mouse Brain Slice Holder (Chamber Height: 4 mm) 1 unit C12007 Whole Rat Brain Holder 1 unit C12101 Multi-Cable 1 unit C12102 X-CLARITY™ Reservoir Cap with Temperature Probe 1 unit Replacements C12103 Snap-Lock Connector Tube 1 unit C12104 Peristaltic Pump Tube 1 unit... -
Page 19: Purchaser Notification
Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents owned or controlled by the Company that cover the product based on the manufacture, use, or sale of a therapeutic, clinical diagnostic, vaccine, or prophylactic product developed in research by the purchaser in which this product or its components were employed, provided that neither this product nor any of its components were used in the manufacture of such product. - Page 20 Logos Biosystems Headquarters Doosan Venturedigm Suite 514 415 Heungan-daero, Dongan-gu Anyang-si, Gyeonggi-do 14059 OUTH OREA Tel: +82 31 478 4185 Fax: +82 31 478 4184 7700 Little River Turnpike Suite 207 Annandale, VA 22003 Tel: +1 703 622 4660 Tel: +1 703 942 8867 Fax: +1 517 266 3925 sales@logosbio.com...
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