SCIEX ExionLC 100 Hardware User's Manual
  1. Manuals
  2. Brands
  3. SCIEX Manuals
  4. Controller
  5. ExionLC 100
  6. Hardware user's manual

SCIEX ExionLC 100 Hardware User's Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
ExionLC
100 System
Hardware User Guide
RUO-IDV-05-1839-C
May 2018

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the ExionLC 100 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for SCIEX ExionLC 100

  • Page 1 ExionLC 100 System Hardware User Guide RUO-IDV-05-1839-C May 2018...
  • Page 2 SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX s sole and exclusive representations, warranties, and obligations.

  • Page 3: Table Of Contents

    Contents 1 Operational Precautions and Limitations....................6 Electrical Precautions.................................6 AC Mains Supply................................6 Protective Earth Conductor............................7 Environmental Precautions..............................7 Electromagnetic Environment............................8 Decommissioning and Disposal............................8 Ventilation Precautions..............................9 Chemical Precautions.................................9 System Safe Fluids..............................10 Static Electricity Precautions ............................11 Typical Cause of Static Electricity Accidents.......................11 Preventing Static Electricity Accidents........................14 Equipment Use and Modification.............................16 Maintenance, Inspections, and Adjustment........................17 Storage and Handling..............................18...
  • Page 4 Contents Set a Safety Lock................................52 Set Password Request to Deactivate the Lock......................54 Configure Network Settings............................57 Set the Date and Time...............................61 Change the System Pass ID............................64 Change the Login Pass ID............................66 Initialize Settings................................69 Configure the System to Shut Down after Analysis....................71 4 Operating Instructions...........................75 Sample Workflow................................75 Turn on the HPLC System..............................76...
  • Page 5 Contents Storage and Handling..............................171 6 Troubleshooting...........................172 Hardware Issues................................172 Analysis Issues................................177 Causes of and Countermeasures for Clogging in Flow Lines..................182 A Consumables, Options, and Spares....................184 Consumables.................................184 Sample Vials for Use with the Autosampler......................185 Septum for Use with the Autosampler........................188 Microtiter Plate................................189 Pump Spares..................................191 Autosampler Spares...............................192 Column Oven Spares..............................193...

  • Page 6: Operational Precautions And Limitations

    The covers need not be removed for routine maintenance, inspection, or adjustment. Contact a SCIEX Field Service Employee (FSE) for repairs that require the covers to be removed. • Follow required electrical safe work practices.

  • Page 7: Protective Earth Conductor

    Operational Precautions and Limitations CAUTION: Potential System Damage. Do not unpack or connect any system components. The FSE will unpack, connect, and configure the system for the proper operating voltage. Guidelines: • Do not connect the wiring in a manner other than that prescribed by the manufacturer. •...

  • Page 8: Electromagnetic Environment

    Storage and Handling. Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE to obtain a copy of the form. Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.

  • Page 9: Ventilation Precautions

    • Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX Safety Data Sheets can be found at sciex.com/tech-regulatory.

  • Page 10: System Safe Fluids

    The following fluids can safely be used with the system. CAUTION: Potential System Damage. Do not use any other fluid until confirmation is received from SCIEX that it does not present a hazard. This is not an exhaustive list. • Organic Solvents •...

  • Page 11: Static Electricity Precautions

    Operational Precautions and Limitations Static Electricity Precautions Liquid chromatography (LC) uses flammable organic solvents as the mobile phase. LC systems are also often used where large amount of flammable substances are present. Thus, there is a risk of accidents involving fire or explosion.
  • Page 12 Operational Precautions and Limitations Table 1-1 Static Electricity Accidents—Sequence of Events Event Result Generation of static activity. When liquid is passed through thin tubing at high flow rates, as in liquid chromatography, the electrostatic charges of the flowing matter generate static electricity.
  • Page 13 Operational Precautions and Limitations Table 1-1 Static Electricity Accidents—Sequence of Events (continued) Event Result Release of energy through If this happens and an electrical conductor is brought within a certain distance of electrical discharge. the container, then an electrical discharge occurs, releasing thermal energy that will ignite any flammable gas of sufficient density in the vicinity.

  • Page 14: Preventing Static Electricity Accidents

    Operational Precautions and Limitations Preventing Static Electricity Accidents The best way to prevent static electricity accidents is to prevent the occurrence and accumulation of electrostatic charges. If large amounts of flammable solvent are collected in a large container, then the preventive measures identified in Preventive Measure One,...
  • Page 15 Operational Precautions and Limitations Item Description Connect to a protective earth terminal or other grounding point of the module. (Preventive Measure One) CAUTION: Potential System Damage. Do not connect the grounding wire to the gas tubing, the water service tubing, or the telephone line. Static electricity generated by the liquid will be conducted through the container to the earth.

  • Page 16: Equipment Use And Modification

    • Use as small a container as possible to minimize damage in the event of fire. Equipment Use and Modification WARNING! Personal Injury Hazard. Contact the SCIEX representative if product installation, adjustment, or relocation is required. ExionLC...

  • Page 17: Maintenance, Inspections, And Adjustment

    WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts not recommended by SCIEX or use of parts for any purpose other than their intended purpose can put the user at risk of harm or negatively impact system performance.

  • Page 18: Storage And Handling

    Operational Precautions and Limitations Storage and Handling WARNING! Environmental Hazard. Do not dispose of system components in municipal waste. Follow local regulations when disposing of components. The environmental requirements for the storage and transport of the ExionLC 100 system: • Altitude not exceeding 1 828 m (6 000 feet) above sea level •...

  • Page 19: Overview

    Overview The ExionLC 100 system is an integrated high performance liquid chromatography system that integrates a pump (quaternary low-pressure gradient), autosampler, and column oven and realizes a comfortable operating environment while automating testing (validation). This system collects the status of each unit of the pump, autosampler, and column oven for executing control. The network function included as standard enables monitoring of the analysis status from the acquisition computer.

  • Page 20: Front Side

    Overview Item Description Controller Autosampler Pump Column oven Front Side Figure 2-2 Front Side ExionLC 100 System Hardware User Guide 20 / 219 RUO-IDV-05-1839-C...
  • Page 21 Overview Item Description Controller panel. Has a status panel for operation using a touch panel, and if the panel is opened. Status indicator. Shows the operating status of the system using different colors of the light that it illuminates. • Green: Analyzable •...

  • Page 22: Side View

    Overview Side View Figure 2-3 Side of Instrument Item Description Suction tubing. Tube for aspiration of the mobile phase or the rinse solution for the autosampler. A suction filter is attached to the end. Reservoir bottle. Contains the mobile phase or the rinse solution for the autosampler. Reservoir tray.
  • Page 23 Overview Item Description Condensation water drain for oven. Drains the condensation water when the oven is set to a maximum. of room temperature. Rinse liquid drain. Drains the rinse solution of the autosampler. Not applicable Main unit power switch. Turns the power to the entire system on or off. Normally it must be turned on.

  • Page 24: Rear Panels

    Overview Rear Panels Figure 2-4 Rear Panels Item Description Optional board. An optional A/D board or an optional optical board can be installed as an optional part. RS232C connector. Unused. ETHERNET connector. Connects the acquisition computer. External input/output terminal. Connects external devices (including the mass spectrometer) and synchronizes using a relay contact.

  • Page 25: Interior View

    Overview Interior View Figure 2-5 Interior Item Description Column oven. An air circulation-style column oven. The temperature of the column is maintained at a given value. High pressure valve. Switches between high-pressure flow lines. Low pressure valve. Switches between the sample liquid and the rinse solution for measuring with the measuring pump.
  • Page 26 Overview Item Description Measuring pump. Aspirates or discharges the predetermined volume of sample and rinse solution. Mixer. Stirs the mobile phase to make it uniform and stabilizes the baseline. Pump. Pumps the mobile phase. Drain valve. Opens the discharge flow line during a manual purge. Low-pressure gradient unit.

  • Page 27: Flow Line Diagram

    Overview Flow Line Diagram Entire Flow Line Figure 2-6 Entire Flow Line Legend Description Low-pressure mobile phase High-pressure mobile phase Flow line direction Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 27 / 219...

  • Page 28: Inner Volume Of Each Flow Line

    Overview Item Description Item Description Mobile Phase Sample Loop Degasser Needle Pump Measuring Pump Low-Pressure Gradient Unit Low-Pressure Valve LPGE-OUT Tube Autosampler Outlet Tubing Pump Head Column Oven Mixer (optional) Column Autosampler Rinsing Port High-Pressure Valve Drain (Mobile Phase Waste Liquid) Inner Volume of Each Flow Line The following table describes the inner volume of each flow line for the main unit and the optional flow line parts.

  • Page 29: Flow Line During Sample Injection Operations

    Overview Flow Line Flow Line Part Name Volume (µL) Location Autosampler outlet tubing Total System Delay Volume Flow Line During Sample Injection Operations Sample injection operations occur in the following order. Ready The mobile phase sent from the pump goes through the high-pressure valve, sample loop, needle, injection port, then back to the high-pressure valve before reaching the column.
  • Page 30 Overview Legend Description Mobile phase Flow line direction Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — ExionLC 100 System Hardware User Guide 30 / 219...

  • Page 31: Release Of Pressure From The Flow Line

    Overview Release of Pressure from the Flow Line The high-pressure valve moves to the load position and releases the pressure in the sample loop. Figure 2-8 Flow Line Legend Description Mobile phase Release of pressure Flow line direction Item Description Item Description Pump...

  • Page 32: Needle Movement

    Overview Item Description Item Description Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — Needle Movement After the low-pressure valve has operated, the needle moves up. Figure 2-9 Needle Movement Legend Description Mobile phase Flow line direction ExionLC 100 System...

  • Page 33: Needle Rinse Before Sample Aspiration

    Overview Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — Needle Rinse Before Sample Aspiration The needle is inserted in the rinse port, and the outside of the needle is rinsed with rinse solution in the rinsing port.
  • Page 34 Overview Legend Description Mobile phase Flow line direction Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — ExionLC 100 System Hardware User Guide 34 / 219...

  • Page 35: Sample Aspiration

    Overview Sample Aspiration When the needle is inserted in the sample vial, the measuring pump is activated and the sample is aspirated in the needle and the sample loop. Figure 2-11 Flow Line Legend Description Mobile phase Aspiration Flow line direction Item Description Item...

  • Page 36: Needle Rinse After Sample Aspiration

    Overview Item Description Item Description Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — Needle Rinse After Sample Aspiration The needle is inserted in the rinsing port, and the outside of the needle is rinsed with rinse solution in the rinsing port.

  • Page 37: Start Analysis

    Overview Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — Start Analysis When the needle is inserted in the injection port, the high-pressure valve shifts to the injecting position and analysis starts.
  • Page 38 Overview Legend Description Mobile phase Flow line direction Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — ExionLC 100 System Hardware User Guide 38 / 219...

  • Page 39: Initialize The Measuring Pump

    Overview Initialize the Measuring Pump After the low-pressure valve has operated, the measuring pump returns to the initial position. Figure 2-14 Mobile Phase Legend Description Mobile phase Flow line direction Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle...

  • Page 40: Rinse Solution Aspiration

    Overview Item Description Item Description Injection Port Sample Column Manual Prime Port Rinse Solution -— — Rinse Solution Aspiration When the needle is inserted in the sample vial, the measuring pump is activated and the sample is aspirated in the needle and the sample loop. Figure 2-15 Flow Line Legend Description...

  • Page 41: Rinse Solution Discharge

    Overview Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — Rinse Solution Discharge After the low-pressure valve has operated, the measuring pump discharges the rinse solution into the rinsing port. Figure 2-16 Flow Line Hardware User Guide ExionLC...
  • Page 42 Overview Legend Description Mobile phase Aspiration Flow line direction Item Description Item Description Pump Low-Pressure Valve High-Pressure Valve Measuring Pump Sample Loop Rinsing Port Needle Drain (Waste Rinse Liquid) Injection Port Sample Column Manual Prime Port Rinse Solution -— — ExionLC 100 System Hardware User Guide...

  • Page 43: Configuration

    Configuration To create the hardware profile for the system, refer to the ExionLC System Software User Guide. Some system configuration tasks can be performed with the touch pad on the controller. Configure Initial Settings Show the Calibration Tab 1. Press (Menu).
  • Page 44 Configuration 2. Press Maintenance. Figure 3-2 Maintenance Button The Maintenance screen is shown. 3. Press (Calibration). ExionLC 100 System Hardware User Guide 44 / 219 RUO-IDV-05-1839-C...
  • Page 45 Configuration Figure 3-3 Calibration Button The Login screen is shown. 4. Type the system pass ID number in the Password field. The factory default pass ID is "00000". Figure 3-4 Login Screen 5. Press OK. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 45 / 219...
  • Page 46 Configuration The Calibration screen is shown. Calibration Tab Use this screen to peform these tasks: • Set part replacement guidelines • Calibrate, teach, and perform other system adjustments • Change the password • Initialize the system Figure 3-5 Calibration Tab Item Label Description...
  • Page 47 Configuration Item Label Description Hardware Calibration Shows the Hardware Calibration screen. This screen allows execution of hardware calibration for each item. System Adjustment Shows the System Adjustment screen. This screen enables rack settings, injection port position adjustment, keypad lock, network settings, date settings, safety lock, and automatic vial detection settings to be configured.
  • Page 48 Configuration Item Label Description Replacement Parts Button Shows the replaceable parts. The parts replacement time guidelines for the applicable part can be set. Unit Selection Tab Switches between replaceable parts lists of the different units. It is not possible to select a unit that has no replaceable parts.
  • Page 49 Configuration Figure 3-8 Hardware Calibration Screen Item Label Description Item Button Shows the items of each hardware calibration. The applicable Hardware Calibration operation screen is shown. Unit Selection Tab Shows the hardware calibration status for the specific unit. Hardware Calibration Operations Screen Use this screen to perform hardware calibration.
  • Page 50 Configuration Figure 3-9 Hardware Calibration Operations Screen Item Label Description Operations Guide Area Shows the buttons used in the operations guide. Entire Procedure Shows the entire performance verification procedure. Stop Cancels the performance verification and closes the screen. Back Returns to the previous step. Next Proceed to the next procedure.
  • Page 51 Configuration Figure 3-10 System Adjustment Screen Item Label Description Rack Setting Shows the Rack Setting screen. The plate type for each rack ID can be specified and rack position adjustment can be performed. Refer to Adjust the Rack Position. Injection Port Teaching Shows the Injection Port Teaching screen.

  • Page 52: Set A Safety Lock

    Configuration Item Label Description Sample Vial Auto Sensing Shows the Sample Vial Auto Sensing screen. Password request to deactivate Shows the Password request to deactivate lock screen. lock This option can be set • When the system starts. • When the lock is canceled. •...
  • Page 53 Configuration The System Adjustment screen is shown. 2. Press Safety Lock. Figure 3-12 Safety Lock Button The Safety Lock screen is shown. 3. Press the switching button and then select Set. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 53 / 219...

  • Page 54: Set Password Request To Deactivate The Lock

    Configuration Figure 3-13 Safety Lock screen Set Password Request to Deactivate the Lock Prerequisite Procedures • Show the Calibration Tab Set whether to show the Login screen when the system starts, the lock is canceled with the Lock deactivating button, or the Upload screen is shown with the Upload button. 1.
  • Page 55 Configuration Figure 3-14 System Adjustment Button The System Adjustment screen is shown. 2. Press Password request to deactivate lock. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 55 / 219...
  • Page 56 Configuration Figure 3-15 Password request to deactivate lock Button The Password request to deactivate lock screen is shown. 3. Press the switching button and select Enabled. ExionLC 100 System Hardware User Guide 56 / 219 RUO-IDV-05-1839-C...

  • Page 57: Configure Network Settings

    Configuration Figure 3-16 Enabled Button Configure Network Settings Prerequisite Procedures • Show the Calibration Tab 1. Press System Adjustment. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 57 / 219...
  • Page 58 Configuration Figure 3-17 System Adjustment Button The System Adjustment screen is shown. 2. Press Network Setting. ExionLC 100 System Hardware User Guide 58 / 219 RUO-IDV-05-1839-C...
  • Page 59 Configuration Figure 3-18 Network Setting Button The Network Setting screen is shown. 3. Configure the network settings. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 59 / 219...
  • Page 60 Configuration Figure 3-19 Network Settings Screen, Page 1 Figure 3-20 Network Settings Screen, Page 2 ExionLC 100 System Hardware User Guide 60 / 219 RUO-IDV-05-1839-C...

  • Page 61: Set The Date And Time

    Configuration Table 3-1 Network Settings Item Description System Name Input the name of the module to be shown on the network. Group Name Input the group name to be joined on the network. IP Address Setting Select whether to automatically obtain an IP address from the server or to set it manually.
  • Page 62 Configuration Figure 3-21 System Adjustment Button The System Adjustment screen is shown. 2. Press Date Setting. ExionLC 100 System Hardware User Guide 62 / 219 RUO-IDV-05-1839-C...
  • Page 63 Configuration Figure 3-22 Date Setting Button The Date Setting screen is shown. 3. Complete the fields in the Date Setting screen. Table 3-2 Fields in the Date Setting Screen Field Description Date Input the date in the Western format. Time Input the time using the 24-hour clock system.

  • Page 64: Change The System Pass Id

    Configuration Change the System Pass ID Prerequisite Procedures • Show the Calibration Tab 1. Press System PassID Setting. Figure 3-23 System PassID Setting Button The System PassID Setting screen is shown. 2. Press ******** and then type a new 5-digit system pass ID Number. ExionLC 100 System Hardware User Guide...
  • Page 65 Configuration Figure 3-24 System Pass ID Setting Screen The message follwing message is shown: Again for confirmation, enter the system pass ID- Number. 3. Press *****, type the new system pass ID Number again, and then press OK. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C...

  • Page 66: Change The Login Pass Id

    Configuration Figure 3-25 System Pass ID Setting Confirmation The system pass ID Number is changed, and the message, New system pass ID- Number is set, is shown. Note: If changing the system pass ID Number fails, this message is shown: The system pass ID-Numberentered does not match the one for verification purposes.
  • Page 67 Configuration Figure 3-26 Login PassID Setting Button The Login PassID Setting screen is shown. 2. Press ******** and then type a new 5-digit login pass ID Number. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 67 / 219...
  • Page 68 Configuration Figure 3-27 Login PassID Setting Screen The message, Again for confirmation, enter the login pass ID- Number, is shown. 3. Press *****, type the new login pass ID Number again, and then press OK. ExionLC 100 System Hardware User Guide 68 / 219 RUO-IDV-05-1839-C...

  • Page 69: Initialize Settings

    Configuration Figure 3-28 Login PassID Setting Screen Confirmation The login pass ID Number is changed, and the message, New login pass ID- Number is set, is shown. Note: If the login pass ID Number is not changed, this message is shown: The login pass ID-Number entered does not match the one for verification purposes.
  • Page 70 Configuration 1. Press Initialization. Figure 3-29 Initialization Button The Initialization Instrument Parameters screen is shown. 2. Press OK. ExionLC 100 System Hardware User Guide 70 / 219 RUO-IDV-05-1839-C...

  • Page 71: Configure The System To Shut Down After Analysis

    Configuration Figure 3-30 Initialization Instrument Parameters The parameters of parameter settings and system settings are initialized, and this message is shown: All parameters have been initialized. Turn the systempower off. Configure the System to Shut Down after Analysis Shutdown can be configured so that the system terminates automatically after analysis completion. It is possible to change the oven temperature and mobile phase concentration and flow rate at the time of shutdown, and to turn off the power to the system components.
  • Page 72 Configuration Figure 3-31 Menu Button The Menu screen is shown. 2. Press Shutdown. ExionLC 100 System Hardware User Guide 72 / 219 RUO-IDV-05-1839-C...
  • Page 73 Configuration Figure 3-32 Shutdown Button The Shutdown Setting screen is shown. 3. Set Shutdown to ON. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 73 / 219...
  • Page 74 Configuration Figure 3-33 Shutdown Screen 4. Select the shutdown-file. 5. To change the setting contents, press anywhere in the content area of the shutdown-file No. The Edit Shutdown File screen is shown. 6. Set the unit parameters after the completion of the analysis. ExionLC 100 System Hardware User Guide...

  • Page 75: Operating Instructions

    Operating Instructions WARNING! Hot Surface Hazard. Do not open the column oven door if the high temperature lamp is blinking. The internal temperature of the column oven is 60 °C or greater. WARNING! Biohazard. Wear personal protective equipment when handling potentially infectious or toxic substances (such as human samples or reagents) in order to prevent contact with the skin.

  • Page 76: Turn On The Hplc System

    Operating Instructions Turn on the HPLC System WARNING! Electrical Shock Hazard. Before connecting the mains supply cable to the mains supply, make sure that the system is turned off. Note: After an emergency, such as a power outage or equipment failure, always inspect the system thoroughly before turning it on.

  • Page 77: Log On To The Hplc System

    Operating Instructions Item Description Power button Power switch The startup screen opens and the system self-check is initiated. If an error occurs, then pump the mobile phase for a while, and then turn the power button off and on. Note: If the power switch is turned off and on, then the HPLC system starts up. Log on to the HPLC System After the system starts, it prompts the user to log in.
  • Page 78 Operating Instructions CAUTION: Potential System Contamination. Do not use resin parts for the high-pressure tubing while pumping at high pressures. Pumping at high pressure might cause resin tubing to be ruptured or disconnected, which could result in mobile phase leaks. Note the maximum withstand pressure of each part when resin parts are used for the high-pressure tubing.

  • Page 79: Select A Rinse Solution

    Operating Instructions CAUTION: Potential System Damage. If PEEK resin parts are used for tubing connections, then do not use the following mobile phases. They weaken PEEK resin, which might lead to cracked tubing and mobile phase leaks. • Concentrated sulfuric acid •...
  • Page 80 Operating Instructions • If the solution precipitates salt when coming into contact with the sample, then use a solution that is similar in composition to the mobile phase and that does not contain salt. • If the component to be analyzed tends to cause the sample to remain on the outside of the needle (for example, if it is an acidic, basic, or ionic material), then use the following rinse solutions: •...
  • Page 81 Operating Instructions • To prevent precipitation of salt, when using a buffer solution with a concentration exceeding 50 mmol/L, keep the concentration of organic solvent in the rinse solution to 50% or less. Refer to Figure 4-2 after injection of the sample, flow lines indicated by solid lines in are filled with rinse solution.
  • Page 82 Operating Instructions Item Description Pump High-pressure valve (for injection) Column Refer to Figure 4-3 before sampling, the high-pressure valve rotates and a portion of mobile phase compressed by high pressure is pushed through the high-pressure valve ports 1 and 2. Depending on the pumping pressure, rinse solution and the mobile phase might be mixed in the area inside the , which might result in salt deposits.
  • Page 83 Operating Instructions Item Description Rinsing port Measuring pump Sample loop Some of the mobile phase and the rinse solution might mix Low-pressure valve Manual syringe Rinse solution Pump High-pressure valve (for loading) Column • For low-pressure gradient analysis using a highly concentrated buffer solution and an organic solvent, replace the tubing from the outlet of the low-pressure gradient unit to the inlet of the pump with the accessory LPGE-OUT tube long.

  • Page 84: Install The Mobile Phase And Rinse Solution On The Reservoir Tray

    Phase. 1. Prepare a mobile phase and then put it into a dedicated reservoir bottle. Tip! SCIEX recommends that the mobile phase be filtered it through a membrane filter with a filter size of 0.45 µm or less. Tip! SCIEX recommends that ultrasonic vibrations for 2 minutes or 3 minutes be used for degassing.

  • Page 85: Perform An Autopurge

    Operating Instructions Item Description Reservoir bottle Tags on suction tubing Degasser ports 2. Put the suction filter and the tube of the mobile phase port to be used in the reservoir bottle. Tags on the suction tubing identify the degasser ports to which the tubing should be connected. Consult the tags and install each tube in the reservoir bottle.
  • Page 86 Operating Instructions • After using a buffer solution as the mobile phase, when replacing or purging the mobile phase with the buffer solution remaining in the column, do not purge with a liquid that easily forms a precipitate in the buffer solution, such as highly concentrated organic solvent.
  • Page 87 Operating Instructions Figure 4-6 Purge Button 3. Turn ON 1st to 4th and Rinse. Keep the default purge time. Replace the entire flow line with mobile phase before setting the column. Note: • The default purge time is three minutes for mobile phases and ten minutes for rinse solutions. If air bubbles are found in the flow line, set the time two to five minutes longer than the initial value.
  • Page 88 Operating Instructions Figure 4-7 1st to 4th and Rinse Turned On 4. Press Start. Note: • Press Stop to cancel the autopurge. • Press Skip to cancel purging of the mobile phase that is currently being purged and start purging of the next mobile phase.
  • Page 89 Operating Instructions Figure 4-8 Purging In Process 5. After the purge finishes, press Close. The Main Analysis screen opens. Figure 4-9 Close Button Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 89 / 219...

  • Page 90: Install The Column

    Operating Instructions 6. Set the flow rate to 1 mL/min and then press Pump to start pumping. The mobile phase in the flow lines of the column oven, which is not replaced through autopurging, is replaced. 7. After 10 minutes, press Pump to stop pumping. •...
  • Page 91 Operating Instructions Required Materials • Male nut fitting • Ferrule • (Optional) UHPLC fitting 1. Open the column oven door. Figure 4-11 Opening the Column Oven Door 2. Attach the column (A) to the column clamps (B) with the outlet side (a) turned up. Hardware User Guide ExionLC 100 System...
  • Page 92 Operating Instructions Figure 4-12 Install Column 3. Connect the tubing to the column. Connect the SUS tubing (B) to the inlet side (a) of the column (A) from the autosampler. Note: For the fittings to be used, refer to Consumables, Options, and Spares.
  • Page 93 Operating Instructions Figure 4-13 Connect Column Oven Tubing 4. Connect the red PEEK tubing from the outlet side of the column to the ion source. 5. Close the column oven door. CAUTION: Potential System Damage. Be sure to lock the column oven door after closing it to prevent the door from being opened by mistake during analysis or from being opened while the tubing inside of the column oven is in contact with the door.
  • Page 94 Operating Instructions Figure 4-14 Columns from 100 mm to 300 mm ExionLC 100 System Hardware User Guide 94 / 219 RUO-IDV-05-1839-C...

  • Page 95: Equilibrate The Column

    Operating Instructions Figure 4-15 Columns up to 100 mm 2. Connect the columns. Equilibrate the Column Adjust the temperature of the column according to the column oven. 1. Set the flow rate to one-fifth the set value on the Main Analysis screen. Note: When the column is at a low temperature, starting pumping might cause an error due to pressure that is too high, or it might damage the column.

  • Page 96: Prepare The Sample

    Operating Instructions Prepare the Sample Put the Sample In a Sample Vial WARNING! Biohazard. Wear personal protective equipment when handling potentially infectious or toxic substances (such as human samples or reagents) in order to prevent contact with the skin. CAUTION: Potential System Damage. Filter the sample in advance using a membrane filter (0.45 µm or less) to remove solid matter and insoluble materials, including dust.
  • Page 97 Operating Instructions Figure 4-16 Sample and Membrane Filter CAUTION: Potential System Damage. When using a sample vial, attach the cap with the PTFE surface of the silicone septum turned down, that is, to the liquid side. If the PTFE surface is turned up, then the sample solvent might melt the silicone rubber. CAUTION: Potential System Damage.
  • Page 98 Operating Instructions Note: When using a sample vial, attach the cap (C) with the PTFE sheet surface (a) (deep color) of the septum (B) turned down. Figure 4-17 Sample Vial Note: When the sample is cooled, the liquid level should be as indicated in the following Figure 4-18.

  • Page 99: Put The Sample In The Autosampler

    Operating Instructions Put the Sample in the Autosampler Two sample racks can be installed in the autosampler at once, and each sample rack can be loaded with two plates of the same type. Plate types include vial plates (1 mL, 1.5 mL, and 4 mL), microtiter plates (MTPs), and deep-well plates (DWPs).
  • Page 100 Operating Instructions Figure 4-20 Installing Sample Plates on the Rack • Vial plate: Install No. 1 in the front left corner. • Microtiter plate and deep-well plate: Install the A01 well in the front left corner. Note: Both plates placed on one sample rack must be of the same type. Placing different types of plates might cause failure in sample injection.

  • Page 101: Removal Of Sample Racks During Analysis

    Operating Instructions Figure 4-21 Main Analysis Screen Name Display Rack Position Plate Type 1 mL, 1.5 mL, 4 mL, MTP96, DWP96, MTP384, DWP384 Removal of Sample Racks During Analysis WARNING! Puncture Hazard. Do not put hands inside the instrument during analysis. The Z mount and the needle continue operating even if a sample rack has been removed.

  • Page 102: Post-Analysis Procedures

    Operating Instructions • Turning off the main power switch on the left side of the main unit during injection of a sample, that is, when the rack LED is flashing, might stop the needle while it is in a lower position. With the needle in a lower position, pulling out the sample rack might cause the needle to become bent.
  • Page 103 Operating Instructions If the power button is pressed for four seconds or more, then the system power goes off. This might cause corruption of the settings data. The Confirmation screen opens. 2. Press OK. 3. Make sure that the power button is orange. 4.

  • Page 104: Service And Maintenance

    Service and Maintenance WARNING! Electrical Shock Hazard. Always turn off the power and then disconnect the instrument prior to performing inspection and maintenance. Otherwise, fire, electric shock, or a malfunction might occur. WARNING! Toxic Chemical Hazard. Before disconnecting parts in the flow line, stop the LC pump and then make sure that the pressure of the mobile phase is decreased to zero.

  • Page 105: Replace The Mobile Phase

    Service and Maintenance Replace the Mobile Phase Note: • It is possible to automatically specify the flow rate and concentration of mobile phase and the oven temperature after analysis finishes. Refer to Configure the System to Shut Down after Analysis. •...
  • Page 106 Service and Maintenance Figure 5-1 Pump Settings Table 5-2 Pump Settings Field Value Pumping Mode Mobile Phase Port Mobile phase port whose tubing is connected to the bottle with the column sealing liquid Flow Rate Setting One-fifth of the analysis flow rate 5.

  • Page 107: Replace The Mobile Phase With A Miscible Solvent

    Service and Maintenance Figure 5-2 Stop Pumping Replace the Mobile Phase with a Miscible Solvent 1. Pour 100 mL of the new mobile phase into a 200 mL beaker. 2. Remove the suction filter from the reservoir and then put the filter into the beaker filled with the new mobile phase in the preceding step.
  • Page 108 Service and Maintenance Item Description Beaker Suction filter Reservoir 3. Shake the filter slightly to mix the mobile phases. 4. Put new mobile phase in the reservoir. 5. Remove the suction filter from the beaker and then put it into the reservoir. 6.
  • Page 109 Service and Maintenance Figure 5-5 Drain the Mobile Phase into the Drain Bottle Item Description Drain valve knob Drain tubing, to waste bottle 8. Disconnect the tubing connecting autosampler outlet to the column, and then put the tubing into the waste container.
  • Page 110 Service and Maintenance 9. Close the drain valve. Turn the drain valve knob clockwise as far as possible. 10. Set the flow rate to 1 mL/min to 3 mL/min. Figure 5-7 Pump Status Panel 11. Press pump. Pumping starts, and the pump indicator illuminates. 12.

  • Page 111: Replace The Rinse Liquid (If Applicable)

    Service and Maintenance Replace a Buffer Solution Used as a Mobile Phase CAUTION: Potential System Damage. After analysis using a buffer solution as the mobile phase, clear the flow line with distilled or HPLC-grade water to prevent blockages in the flow line caused by crystals formed due to dehydration of the buffer solution.
  • Page 112 Service and Maintenance Figure 5-8 Preparing for AutoPurge 2. Remove the cap (B) from the rinse solution bottle (A) in the automatic rinsing kit and add then 1 L of 1:9 isopropanol:HPLC-grade water. Figure 5-9 Rinse Bottle Cap Removal ExionLC 100 System Hardware User Guide 112 / 219...
  • Page 113 Service and Maintenance 3. Attach the cap (B) of the automatic rinsing kit to the rinse solution bottle (A), and then remove the outlet FEP tubing (E), in the upward U-shape, from the rinse solution bottle. Figure 5-10 Automatic Rinsing Kit Connections 4.
  • Page 114 Service and Maintenance Figure 5-11 Pump Flow Rate: 3 mL/min 7. Press Pump. Figure 5-12 Pump Button: Turn on Pump Pump is illuminated in blue and pumping starts. 8. Make sure that the rinse liquid flows from the end of the FEP tube on the outlet side of the automatic rinsing kit.

  • Page 115: Purge The Pump Manually

    Service and Maintenance 9. If the rinse liquid is flowing, press Pump. Figure 5-13 Pump Button: Turn off Pump Pump is turned off, and pumping stops. 10. Return the automatic rinsing kit to the original status. Purge the Pump Manually Prerequisite Procedures •...
  • Page 116 Service and Maintenance Figure 5-14 Syringe Connection WARNING! Crushing Hazard. Be careful not to pinch fingers when opening or closing the doors. 3. Open the pump door, rotate the drain valve knob (A) 90 degrees counter-clockwise, and then open the drain valve.
  • Page 117 Service and Maintenance Figure 5-15 Drain Valve 4. Configure the pump settings. Figure 5-16 Pump Settings Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 117 / 219...
  • Page 118 Service and Maintenance Table 5-3 Pump Settings Field Value Pumping Mode Mobile Phase Port Mobile phase port whose tubing is connected to the bottle with the column sealing liquid Flow Rate Setting 0.0001 mL/min 5. Press Pump. The pump operates and the valve of the set mobile phase port opens. Figure 5-17 Manual Purge in Process 6.

  • Page 119: Prime The Autosampler Rinse Solution Manually

    Service and Maintenance Figure 5-18 Drain Valve 10. Close the pump door. 11. Remove the syringe. 12. Perform an autopurge for 3 minutes or more. Refer to Perform an Autopurge. Perform an autopurge for all of the mobile phase ports for which manual purging was performed until there are no air bubbles in the flow line.
  • Page 120 Service and Maintenance Note: When replacing with a non-miscible solvent, first replace with a miscible solvent as an intermediate rinse solution, and then replace with the required solvent. 1. Open the valve mount door. Figure 5-19 Valve Mount Door 2. Insert the syringe with a needle (A) into the tube coming out of low-pressure valve port No. 3 (B). ExionLC 100 System Hardware User Guide...
  • Page 121 Service and Maintenance Figure 5-20 Syringe Installed 3. Press Purge on the Main Analysis screen. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 121 / 219...
  • Page 122 Service and Maintenance Figure 5-21 Purge Button The Auto Purge screen is shown. 4. Press Manual Prime. ExionLC 100 System Hardware User Guide 122 / 219 RUO-IDV-05-1839-C...
  • Page 123 Service and Maintenance Figure 5-22 Prime Button The flow line switches for manual priming. 5. Pull the plunger of the syringe with needle (A) and aspirate the rinse solution. The criterion value for the aspiration volume is 5 mL. 6. Press Close to finish manual priming. The Auto Purge screen is shown.

  • Page 124: Change Sample Racks

    Service and Maintenance Change Sample Racks The system is provided with 1.5 mL sample vial plates as a standard. This section provides the procedure for changing the settings to use other plates. Set the Rack ID The rack ID is the ID the module uses to identify the type of plate that has been inserted. Set the rack ID by operating the sliding switch at the rear of the sample rack.
  • Page 125 Service and Maintenance 2. Inspect the label (a) in front of the sample rack to verify the sliding switch position (b) of the rack ID to be set. Figure 5-24 Label and Sliding Switch Positions 3. Set the sliding switch position (c) at the back of the sample rack. Note: Move the sliding switch forward or backward after pushing the projection a little.
  • Page 126 Service and Maintenance Figure 5-25 Rack ID Table Seven different rack types can be selected (A to G) ExionLC 100 System Hardware User Guide 126 / 219 RUO-IDV-05-1839-C...
  • Page 127 Service and Maintenance Table 5-4 Rack Types Rack ID Sliding Switch Position Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 127 / 219...
  • Page 128 Service and Maintenance Table 5-4 Rack Types (continued) Rack ID Sliding Switch Position ExionLC 100 System Hardware User Guide 128 / 219 RUO-IDV-05-1839-C...
  • Page 129 Service and Maintenance Table 5-4 Rack Types (continued) Rack ID Sliding Switch Position Labels to Affix to the Front of the Handle The sample rack is supplied with labels to be put on the handle. When necessary, apply them to a location that can be easily seen, such as the front of the handle.

  • Page 130: Install The Plates On The Sample Rack

    Service and Maintenance Figure 5-26 Rack Labels Install the Plates on the Sample Rack CAUTION: Potential System Damage. Make sure that the plate type, needle stroke, and rack position are properly configured. If the configured plate type does not match the type of the plate installed, then the needle might strike the plate, instead of descending into the well, resulting in damage to the needle.
  • Page 131 Service and Maintenance Figure 5-27 1.5 mL Sample Vial Plate Table 5-5 Indications and Positions of Plate Identification Panels Plate Type Display Position 1 mL sample vial plate 1 mL Front 1.5 mL sample vial plate 1.5 mL Front 4 mL sample vial plate 4 mL Front 96-Well microtiter plate...
  • Page 132 Service and Maintenance Figure 5-28 Panel Alignment 2. Install two plates (A) of the same type on the sample rack. ExionLC 100 System Hardware User Guide 132 / 219 RUO-IDV-05-1839-C...
  • Page 133 Service and Maintenance Figure 5-29 Install Plates on the Sample Rack 3. Install the sample rack (A). Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 133 / 219...

  • Page 134: Open The Rack Setting Screen

    Service and Maintenance Figure 5-30 Sample Rack Installation Note: If the same rack ID is set for both the left and right racks, the following error message is shown: Racks that have the same ID are set in the autosampler. Change either the left or right rack ID.
  • Page 135 Service and Maintenance Figure 5-31 Menu Button The Menu screen is shown. 2. Press Maintenance. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 135 / 219...
  • Page 136 Service and Maintenance Figure 5-32 Maintenance Button The Maintenance screen is shown. 3. Press (Calibration). The Login screen is shown. 4. Type the system pass ID Number and then press OK. Refer to Log on to the HPLC System. The Calibration screen is shown. 5.
  • Page 137 Service and Maintenance Figure 5-33 System Adjustment Button The System Adjustment screen is shown. 6. Press Rack Setting. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 137 / 219...

  • Page 138: Set The Plate Type And Needle Stroke From The Top

    Service and Maintenance Figure 5-34 Rack Setting Button The Rack Setting screen is shown. Set the Plate Type and Needle Stroke from the Top Prerequisite Procedures • Open the Rack Setting Screen 1. Press to select a rack ID. 2. Press The Rack Setting screen is shown.
  • Page 139 Service and Maintenance Table 5-6 Plate Type and Needle Stroke from Top Plate Type Screen Display Needle Stroke from the Top Setting Range Initial Value 1 mL sample vial plate 1 mL 10 mm to 52 mm 47 mm 1.5 mL sample vial plate 1.5 mL 10 mm to 52 mm 47 mm...
  • Page 140 Service and Maintenance Note: When using a microtiter plate: If the needle stroke is set to 45 mm, the initial value, then the tip of the needle is designed to come down about 6 mm from the well bottom when the well bottom thickness of the plate is 1 mm.
  • Page 141 Service and Maintenance Note: When using a 1.5 mL vial plate: If the needle stroke is set to 47 mm, the initial value, then the tip of the needle is designed to come down to about 2 mm from the vial bottom when the vial bottom thickness is 1.5 mm.

  • Page 142: Adjust The Rack Position

    Service and Maintenance Table 5-7 Residual Volume in Bottom 2 mm of Sample Vial and Reagent Bottle Part Volume Part Number Residual Volume in Bottom 2 mm of Reagent Bottle 1 mL sample vial plate 1 mL 10 mm to 52 mm 47 mm 1.5 mL sample vial plate 1.5 mL...
  • Page 143 Service and Maintenance Figure 5-37 Position Number and Adjustment Position Position Adjustment Number Shown on Plate Number Position 1 mL 1.5 mL 4 mL 96 Hole 384 Hole Sample Vial Sample Vial Sample Vial MTP/DWP MTP/DWP Front Left Corner of the Front Plate Front Right Corner of the...
  • Page 144 Service and Maintenance Position Adjustment Number Shown on Plate Number Position 1 mL 1.5 mL 4 mL 96 Hole 384 Hole Sample Vial Sample Vial Sample Vial MTP/DWP MTP/DWP Rear Left Corner of the Front Plate Front Left Corner of the Rear Plate Note: When adjusting the rack position for sample vial plates, insert the sample vials into all the adjustment positions.
  • Page 145 Service and Maintenance 3. Remove the inserted sample racks (A) and open the panels and doors (B). Figure 5-39 Remove Racks and Open Panels and Doors 4. Remove the 4 knurled screws (A), pull the front panel (B) forward to the right, disconnect the tube from the left slot of the front panel (B), and then detach the front panel (B).
  • Page 146 Service and Maintenance Figure 5-40 Detach the Front Panel 5. Attach the rack teaching jig to the Z mount. Install the rack teaching jig (B) in the threaded screw hole (a) at the bottom of the left side of the Z mount by aligning the jig hole and the screw hole using the cap/knob (A). ExionLC 100 System Hardware User Guide...
  • Page 147 Service and Maintenance Figure 5-41 Install Rack Teaching Jig 6. Select the rack position (L/R) to be adjusted. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 147 / 219...
  • Page 148 Service and Maintenance Figure 5-42 Select the Rack Position 7. Put the plates (A) on the sample rack. ExionLC 100 System Hardware User Guide 148 / 219 RUO-IDV-05-1839-C...
  • Page 149 Service and Maintenance Figure 5-43 Install Plates on the Sample Rack CAUTION: Potential System Damage. Do not attach the sample vial cap when adjusting the 4 mL vial plate position. The cap might make contact with the teaching jig, which can cause errors.
  • Page 150 Service and Maintenance Figure 5-44 Sample Rack Installation 9. Press Next. The Position1 Adjustment screen is shown, and the needle moves to Position1. 10. Press Parallel movement and Vertical movement to move the tip of the needle (A) to the center of the positioning hole (a).
  • Page 151 Service and Maintenance Figure 5-45 Parallel and Vertical Movement Buttons Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 151 / 219...
  • Page 152 Service and Maintenance Figure 5-46 Needle and Positioning Hole Tip! • Use Vertical movement to drop the tip of the needle to a height that makes it easy to verify the relative position between the needle tip and the left-right and front-backpositions of the hole. •...
  • Page 153 Service and Maintenance Table 5-8 Needle Distances Moved Button Direction Operation Left-Right and The needle moves in 0.1 mm increments. Front-Back The needle moves to the highest point. Down First needle drop: 4.0 mm Second and subsequent drops: 0.2 mm Down (Large) First needle drop: 4.0 mm Second and subsequent drops: 1.0 mm...
  • Page 154 Service and Maintenance Figure 5-47 Next Button After adjustment on the Position4 screen is completed, and Next is pressed, the following confirmation screen is shown. ExionLC 100 System Hardware User Guide 154 / 219 RUO-IDV-05-1839-C...
  • Page 155 Service and Maintenance Figure 5-48 Confirmation Screen Tip! Press Cancel to close the message without saving the data. 13. Press OK. The following message is shown: Remove the teaching jig. CAUTION: Potential System Damage. After the rack position adjustment is finished, remove the rack teaching jig and cap/knob.
  • Page 156 Service and Maintenance Figure 5-49 Remove the Rack Teaching Jig 15. Press Close. ExionLC 100 System Hardware User Guide 156 / 219 RUO-IDV-05-1839-C...
  • Page 157 Service and Maintenance Figure 5-50 Close Button 16. Remove the sample rack. 17. Align the groove (b) on the bottom of the front panel with the protrusion on the main body frame (a) to insert the front panel. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C...
  • Page 158 Service and Maintenance Figure 5-51 Attaching the Front Panel 18. Insert the bundle of tubes (C) into the opening (c) on the left side of the front panel. 19. Attach the 4 knurled screws (A). Note: If the front panel (B) is not attached correctly, then condensation might settle on the rack plate. 20.

  • Page 159: Connect The System To A Computer

    Service and Maintenance Figure 5-52 Installing the Racks and Closing the Doors Connect the System to a Computer Required Materials Description Straight LAN cable Min. Category5 UTP straight cable Switching Hub 100 Base-TX compatible Cable Holder Required when wiring a LAN cable on the floor or wall 1.

  • Page 160: Determine The Amount Of Mobile Phase And Rinse Solution Remaining

    Service and Maintenance Figure 5-53 Connection to the Computer 2. Attach the straight LAN cable (A) connected to the module to the switching hub (C) connected to the PC (B). Determine the Amount of Mobile Phase and Rinse Solution Remaining The remaining amount of mobile phases and rinse solution can be determined.
  • Page 161 Service and Maintenance Figure 5-54 Menu Button The Menu screen is shown. 2. Press Mobile Phase Reserve Volume. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 161 / 219...

  • Page 162: Lock The System

    Service and Maintenance Figure 5-55 Mobile Phase Reserve Volume Button The Mobile Phase & Rinse Reserve Volume Setting screen is shown. 3. Configure the initial capacity and warning settings. Lock the System Prerequisite Procedures • Show the Calibration Tab 1. Press System Adjustment. ExionLC 100 System Hardware User Guide...
  • Page 163 Service and Maintenance Figure 5-56 System Adjustment Button The System Adjustment screen is shown. 2. Press Key Lock. Hardware User Guide ExionLC 100 System RUO-IDV-05-1839-C 163 / 219...
  • Page 164 Service and Maintenance Figure 5-57 Key Lock Button The Key Lock screen is shown. 3. Press the switching button and then select Set. ExionLC 100 System Hardware User Guide 164 / 219 RUO-IDV-05-1839-C...

  • Page 165: Clean The Leak Sensor And Leak Tray

    Service and Maintenance Figure 5-58 Key Lock Screen The Login Confirmation screen is shown, and screen operation is prohibited unless the login password is typed. Clean the Leak Sensor and Leak Tray Wipe away any dirt and liquid adhering to the sensor to keep the leak sensor sensitivity at a normal level. •...
  • Page 166 Service and Maintenance Figure 5-59 Location of the Leak Sensor and Tray in Each Module Item Name Item Name Leak Sensor of the High-Pressure Not applicable Valve*1 High-Pressure Valve Leak Tray*1 Column Oven Leak Tray Pump Leak Sensor Column Oven Condensation Water Tray Pump Leak Tray Low-Pressure Valve Leak Sensor...

  • Page 167: Clean The Automatic Rinsing Kit (If Applicable)

    Service and Maintenance Note: If buffer solution was used as mobile phase solution, then crystals formed from dried leaked buffer solution might cause clogging in the drain of the leak tray. If buffer solution has leaked, then quickly wipe away the liquid accumulated in the leak tray, and if the leak tray gets dirty with buffer solution crystals, then wipe it with water.

  • Page 168: Clean The Monitor Screen

    Service and Maintenance Figure 5-60 Column Clean the Monitor Screen WARNING! Toxic Chemical Hazard. If the liquid crystal screen is damaged, then avoid exposure to liquid crystal material. Follow all safety guidelines for handling, storing, and disposing of chemicals. CAUTION: Potential System Damage. Use only the recommended cleaning methods and materials to avoid damaging the equipment.
  • Page 169 Service and Maintenance The monitor screen is a sensitive electronic part. If the screen gets dirty, then use this procedure to remove the dirt. • Wipe the screen lightly with a soft rag or a rag soaked with a neutral detergent or a small amount of ethanol. Hardware User Guide ExionLC 100 System...

  • Page 170: Clean The Module Surfaces

    Service and Maintenance Figure 5-61 Monitor Screen Clean the Module Surfaces Required Materials • Dry, soft rags, or tissue paper • For persistent stains • Diluted, neutral detergent • Water 1. Wipe the module surfaces with the rag or tissue paper. ExionLC 100 System Hardware User Guide...

  • Page 171: Storage And Handling

    Service and Maintenance 2. If the stains persist, then follow these steps: a. Moisten a rag in the diluted, neutral detergent and then wring it dry. b. Wipe the module surfaces, scrubbing as necessary to remove the stains. c. Moisten a rag in water and then wring it dry. d.

  • Page 172: Troubleshooting

    Hardware Issues Issue Possible Cause Corrective Action A burning smell is The instrument has Turn off the module and then contact SCIEX. Refer to detected. malfunctioned. Turn Off the System. Even if the main power The power cord is not Plug in the power cord properly.
  • Page 173 Troubleshooting Issue Possible Cause Corrective Action Air comes in from the Firmly connect the suction filter fitting. connection of the suction filter and the pump. The check valve is Rinse the check valve by pumping isopropanol at a low malfunctioning. flow rate of approximately 0.20 mL/minute.
  • Page 174 Troubleshooting Issue Possible Cause Corrective Action Pumping is unstable and Air bubbles have entered Press Purge to wash away the mobile phase the pulsating flow of the the suction filter tube. completely. Vibrate the suction filter to remove the air pump is excessive.
  • Page 175 Troubleshooting Issue Possible Cause Corrective Action The column is clogged. Inspect the pressure of the column, and if the column is clogged, then replace it. The flow line is clogged. Backwash the flow line. Inspect the flow line, and if it is clogged, then replace the part.
  • Page 176 Troubleshooting Issue Possible Cause Corrective Action The rotation position of the Contact an FSE. high-pressure valve is misaligned. The flow line is clogged. Backwash the flow line. Inspect the flow line, and if it is clogged, replace the part. A time program cannot be The system configuration Make sure that the LC method is configured correctly.

  • Page 177: Analysis Issues

    Troubleshooting Issue Possible Cause Corrective Action A link to an external The connection with, or • Inspect for dirt or foreign particles in the optical cable detector cannot be settings for, an external connector. established even though it detector are incorrect. •...
  • Page 178 Troubleshooting Issue Possible Cause Corrective Action The needle or tubing is • Backwash the high-pressure valve. clogged. • Replace the needle or the tubing if backwashing has no effect. • Rinse the inside of the needle with mobile phase solution. •...
  • Page 179 Troubleshooting Issue Possible Cause Corrective Action The mobile phase solution Make sure that the pump operates normally, and if there is not flowing. is an abnormality, take corrective action. The required amount of Add the required amount of sample to the sample vial. sample is not present in the sample vial.
  • Page 180 Troubleshooting Issue Possible Cause Corrective Action A ghost peak occurs. The tubing, line filter, or Inspect the suction filter for clogging. Additionally, suction filter is dirty. rinse according to the following procedure. 1. Deliver a 17% phosphoric acid solution at a flow rate of 1 mL/min for about 30 minutes.
  • Page 181 Troubleshooting Issue Possible Cause Corrective Action The baseline undulates There are air bubbles inside Press Purge to remove air bubbles. Alternatively, depending on the pump the pump head. connect a syringe to the end connection of the drain stroke. tubing to remove the air bubbles. Refer to Purge the Pump Manually.

  • Page 182: Causes Of And Countermeasures For Clogging In Flow Lines

    Troubleshooting Causes of and Countermeasures for Clogging in Flow Lines Cause of Clogged Tubing Corrective Action Insoluble matter in the mobile phase. Especially when a buffer solution is used as the mobile phase, filter the buffer solution before use with a commercially available strainer that has a membrane filter with a bore diameter of 0.45 µm maximum.
  • Page 183 Usually a thin membrane of solvent-resistant material (such as PTFE) is coated on the septum surface, but if a septum that has not been recommended by SCIEX is in use, then when the needle penetrates the septum, septum shavings might be peeled off, which can cause clogging.

  • Page 184: A Consumables, Options, And Spares

    Consumables, Options, and Spares Order the parts provided in the tables, or their equivalent. Consumables Part Name Part No. Remark Power Cord (for UL/CSA) 071-60821-08 Power Cord (for EU) 071-60825-51 Cable, LD-CT/BU2/RS 4465787 Drain Tube ASSY 228-56223-41 Drain Tube, Silicone 228-25162-04 PTFE Tubing, OD 7.0 x ID 6.0 4412687...

  • Page 185: Sample Vials For Use With The Autosampler

    Consumables, Options, and Spares Sample Vials for Use with the Autosampler To prevent the septum from falling off during injection, tighten the cap before using. Part Name Volume Material Part Application Conforming Description Number Plate 4 mL Sample Vial 4 mL Borosilicate 4413362 General...
  • Page 186 Consumables, Options, and Spares Part Name Volume Material Part Application Conforming Description Number Plate 1 mL Sample 1 mL Vial: 4425150 General / small 1.5 mL sample With cap, 200 Vial Polypropylene volumes vial plate pieces Cap: Disposable Polyethylene 0.3 mL Sample 300 µL Borosilicate 4412816...
  • Page 187 Consumables, Options, and Spares Sample Vial Material for Use with the Autosampler Note: Both glass and plastic (polypropylene) sample vials are available for use with the autosampler. However, to prevent any issues during analysis, follow these precautions when selecting the type of sample vial. Type Characteristic Appropriate Sample Solvent...

  • Page 188: Septum For Use With The Autosampler

    Consumables, Options, and Spares Septum for Use with the Autosampler Part Name Appearance Material Part Conforming Sample Description Number Vial Silicone Silicone rubber 4412734 • 1.5 mL sample vial 100 pieces Rubber w/PTFE cover (228-15652-92) Septum • 1.1 mL sample vial (228-21283-91) •...

  • Page 189: Microtiter Plate

    Consumables, Options, and Spares Microtiter Plate Figure A-1 Microtiter Plate The microtiter plates, deep-well plates, and plate mats recommended for this system are as follows: Table A-1 Well-fitted Sealings and Compatible Plates Plate Type Product Contact Description Microtiter Plate 267245 series Nalge Nunc International Material: Polypropylene (Thermo Fisher Scientific)
  • Page 190 Consumables, Options, and Spares Table A-2 Heat Sealing Mats and Compatible Plates Plate Type Product Contact Description Microtiter Plate 4titude 4Ti-0110 AB gene Material: Polypropylene Volume: 0.3 mL Deep-Well Plate 4titude 4Ti-0130 AB gene Material: Polypropylene Volume: 1.85 mL ABgene Easy Peel AB-0745 AB gene Material: Foil 4titude Peel-Seal 4Ti-0521...

  • Page 191: Pump Spares

    Consumables, Options, and Spares Table A-3 Adhesive Sealing Mats and Compatible Plates (continued) Plate Type Product Contact Description AXYGEN P-DW-20-C Greiner Material: Polypropylene Volume: 2 mL USA SCIENTIFIC NAL-96 USA Scientific Material: Sealing Film 2923-5000 Polyethylene (upper layer) Polypropylene (lower layer) •...

  • Page 192: Autosampler Spares

    Consumables, Options, and Spares Autosampler Spares Part Name Part Number Description Sample Loop (50 µL) 5039278 This is the sample loop for 50 µL injections. Sample Rack 228-55735-41 Additional sample rack. Useful for switching quickly between multiple types of plates. 1 mL Sample Vial Plate (2 228-56197-41 Plate that holds eighty-four 1 mL sample vials.

  • Page 193: Column Oven Spares

    Consumables, Options, and Spares Column Oven Spares Part Name Part Number Description Column Clamp ASSY B5 4412791 A suite of clamps to add a column with an outside diameter between 6.4 mm and 9.5 mm. Column Clamp ASSY B8 4412792 A suite of clamps to add a column with an outside diameter between 9.5 mm and 12.7 mm.
  • Page 194 Consumables, Options, and Spares Part Name Part Number Description 1 L Mobile Phase Bottle (5 228-38583-42 A set of five 1 L reservoir bottles to put mobile phase pcs) Grounding wire with clip 4413363 For static electricity preventative measure one Caps for 18-L or 4-L 228-21354-91 For static electricity preventative measure two...

  • Page 195: B Error Messages

    Error Messages Error Code Error Message Type / Cause Recommended Action 0x0046 Operation mode is Warning. Shown when the Set the external detector (RF local. Set the operation external detector (RF RID) is set RID) to the remote mode. mode to remote. to the local mode.
  • Page 196 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9020 An undefined fatal Fatal error. Determine whether the error has occurred. external detector shows an error and take the corrective action provided in the instruction manual for the external detector.
  • Page 197 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9050 The buffer memory of Warning. The buffer memory for Inspect the network the controller has transmission from the system to connection status. If the error 0x9051 overflowed. the workstation is full. cannot be removed, contact the FSE.
  • Page 198 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9063 An error occurred in Warning. An error occurred in the Clear the error. If the error communication communication between the occurs again, contact the FSE. between the autosampler or column oven and autosampler or column the controller, but the oven and controller.
  • Page 199 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9078 A rack number that is Error 3 Clear the error and perform different from the rack analysis again. number set in the sequence might have been notified to the instrument.
  • Page 200 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9101 The remaining amount Warning Replace the mobile phase of Mobile Phase A-1 and then set the mobile liquid is low. phase volume again. 0x9102 The remaining amount Warning Replace the mobile phase of Mobile Phase B-1 and then set the mobile...
  • Page 201 Error Messages Error Code Error Message Type / Cause Recommended Action 0x910D The consumable part Fatal error count data of the pump is corrupted. 0x910E The pump pressure Error 1. The pump pressure Inspect the flow line for exceeded the exceeded the maximum pressure.
  • Page 202 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9113 The remaining amount Warning Replace the mobile phase of Mobile Phase B and then set the mobile liquid is low. phase volume again. 0x9114 The remaining amount Warning Replace the mobile phase of Mobile Phase C and then set the mobile...
  • Page 203 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9203 Could not detect the Fatal error Press the Power OFF rotation direction button in the error window home position of the to turn the power to the autosampler measuring system off, and then contact pump.
  • Page 204 Error Messages Error Code Error Message Type / Cause Recommended Action 0x920F Temperature control is Fatal error Press the Power OFF turned off because the button in the error window temperature sensor in to turn the power to the autosampler rack L is system off, and then contact malfunctioning.
  • Page 205 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9219 The front panel of the Fatal error 1. Make sure that the front autosampler might not panel is properly be attached correctly. installed. Alternatively, the 2. If the problem is not sensor that detects the resolved, then press position of the front...
  • Page 206 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9220 The system could not Fatal error Press the Power OFF detect the Z- direction button in the error window home position of the to turn the power to the autosampler needle.
  • Page 207 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9227 Temperature control is Fatal error Press the Power OFF turned off because the button in the error window temperature sensor in to turn the power to the autosampler rack plate system off, and then contact L has detected an the FSE.
  • Page 208 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9230 The setting for the Error 3. In the sequence, a sample Verify the sequence. autosampler sample vial number outside the setting Alternatively, make sure that vial number is outside range has been set for the the plate is properly installed.
  • Page 209 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9344 The system could not Error 3. The recovery failed during Verify the communication resend the data an attempt to resend the environment and operating because the buffer transmission data from the status between the system memory of the detector system to the workstation.
  • Page 210 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9401 Temperature control is Error 2 Take action in the following turned off because order. liquid leak was 1. If organic solvent leaks detected inside the inside the column oven, column oven.
  • Page 211 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9406 Temperature control is Fatal error Press the Power OFF turned off because the button in the error window Peltier unit of the to turn the power to the column oven has system off, and then contact detected an abnormal...
  • Page 212 Error Messages Error Code Error Message Type / Cause Recommended Action 0x940B Temperature control is Error 1. The column oven Configure the settings so that turned off because the temperature has exceeded the set the set value for the column temperature of the value for the [Temperature oven temperature simple...
  • Page 213 Error Messages Error Code Error Message Type / Cause Recommended Action 0x9410 Temperature control is Fatal error Press the Power OFF turned off because the button in the error window heater of the column to turn the power to the oven is malfunctioning.

  • Page 214: C Glossary Of Symbols

    Authorized representative in the European community Biohazard CE Marking of Conformity cCSAus mark. Indicates electrical safety certification for Canada and USA. Catalogue number Caution Note: In SCIEX documentation, this symbol identifies a personal injury hazard. ExionLC 100 System Hardware User Guide 214 / 219 RUO-IDV-05-1839-C...
  • Page 215 Glossary of Symbols Symbol Description China RoHS Caution Label. The electronic information product contains certain toxic or hazardous substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and indicates the number of calendar years the product can be in operation. Upon the expiration of the EFUP, the product must be immediately recycled.
  • Page 216 Symbol Description Fragile Fuse Hertz High Voltage. Electrical Shock Hazard If the main cover must be removed, contact a SCIEX representative to prevent electric shock. Hot Surface Hazard In Vitro Diagnostic Device Ionizing Radiation Hazard Keep dry. Do not expose to rain.
  • Page 217 Glossary of Symbols Symbol Description Moving Parts Hazard Pinch Hazard Pressurized Gas Hazard Protective Earth (ground) Puncture Hazard Puncture Hazard Reactive Chemical Hazard Serial number Toxic Chemical Hazard Transport and store the system within 66 kPa to 103 kPa. Transport and store the system within 75 kPa to 101 kPa. Transport and store the system within 10% to 90% relative humidity.
  • Page 218 Glossary of Symbols Symbol Description Transport and store the system within –30 °C to +45 °C. Transport and store the system within –30 °C to +60 °C. USB 2.0 connection USB 3.0 connection Ultraviolet Radiation Hazard Volt Ampere (power) Volts (voltage) WEEE.

  • Page 219: D Glossary Of Warnings

    Glossary of Warnings Note: If any of the labels used to identify a component become detached, contact an FSE. Label Translation (if applicable) FOR RESEARCH USE ONLY. NOT FOR USE IN FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. DIAGNOSTIC PROCEDURES.

Table of Contents