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PIP 120260 Operating Manual

Single channel fixed volume pipettes
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  • Page 2 Single Channel Fixed Volume Pipettes Institute of Global Certification Institute of Global Certification ACCREDITED ® CERTIFIED CERTIFIED Management ISO 13485:2016 ISO 9001:2015 systems Certification Body MSCB 105 Address: First Floor, Number 12, Naghdi St., Jahantab St., Motahari Ave., Tehran, Iran ZIP Code: 1576635714 +98 912 3340197 P.O.Box:...
  • Page 3 National Health, Not Only Our Job But Also, Our Mission Pole Ideal Pars )P.I.P .( is a manufacturing company, providing products that are designed to promote innovation and improve productivity, thanks to the expertise and long-time experience. We benefit from skilled and dedicated employees with great passion who played a significant role in research and development of new products, strict quality control, and improving customer satisfaction.
  • Page 4 A Good Beginning Makes a Good Ending P.I.P. pipettes are designed to deliver superior performance and accuracy year after year. The high quality and long-term reliability of P.I.P. pipettes are highly based upon the design, the quality of components and the entire controlled production process.

  • Page 5: Table Of Contents

    Contents Introduction ............6 P.I.P. Pipette Description ......8 Pipetting Techniques ........12 Proper Pipetting Advices .......16 Cleaning and Maintenance ......20 Decontamination ..........21 Autoclaving ............23 Pipette Service ...........24 Shipment ............26 P.I.P . Pipette Serial Number ......27 P.I.P. Pipette Warranty ........28 Choosing the Proper Pipette ......30 P.I.P .
  • Page 6 POLE IDEAL PARS...

  • Page 7: Introduction

    www.medpip.com 1. Introduction Read this Operating Manual completely before using P.I.P . pipettes for the first time. This Operating Manual contains all the information required for pipette maintenance, service intervals, decontamination, etc. So, keep it safe and handy. Enclose this Operating Manual when transferring the pipette to third parties. Pole Ideal Pars Co.
  • Page 8 1-2. Symbols : Caution : Important points 1-3. Delivery Package Each P.I.P . pipette box contains the following objects: Table 1. P.I.P . Pipette Delivery Package Product Qty. Pipette Operating Manual P.I.P. Certificate Spare O-ring Guarantee Card Mini Brochure...

  • Page 9: Pipette Description

    www.medpip.com 2. P.I.P. Pipette Description 2-1. P.I.P. Pipette Overview Push Button Tip ejector Button Ergonomic Finger Hook Robust and Lightweight Body Tip ejector Metal Tip Cone with O-rings for optimal sealing and precision Figure 1. P.I.P . Pipette Overview...
  • Page 10 2-2. Features P.I.P. pipettes offer maximum reliability and comfort for applications where accuracy and precision are essential. Built with unique features such as: 2-2-1. Pipetting System Metal pipette parts, such as the tip cones, prevent breakage resulting from accidental dropping and offer superior durability of the pipette. The leak-tight O-rings prevent leakage and provide precise results.
  • Page 11 www.medpip.com 2-2-4. Body High-Quality and robust body of P.I.P . pipette protects internal components from damage if dropped. Lightweight and ergonomic body of the P.I.P . pipette with balanced weight distribution offers greatest comfort for extended pipetting periods. P.I.P . pipettes are resistant to UV radiation and common laboratory chemicals, organic solvents and detergents.
  • Page 12 2-3. Working Principles P.I.P . pipette operates according to air cushion principles )air displacement pipettes(. There is always a cushion of air )dead volume( between the piston and the liquid. When the push button is pressed down, the piston inside the instrument moves down to let air out.

  • Page 13: Pipetting Techniques

    www.medpip.com 3. Pipetting Techniques In order to pipette different samples by P.I.P . pipettes, 2 main pipetting techniques can be used: 1. Forward Pipetting Mode. 2. Reverse Pipetting Mode. Use Forward Pipetting Mode for aqueous solutions. Use Reverse Pipetting Mode for volatile and viscous samples. Refer to table 2 for Proper Pipetting Techniques.
  • Page 14 3-1. Forward Pipetting Rest Position First Stop Second Stop Figure 6. Forward Pipetting 1. Preparation: Hold the pipette in a vertical position. Press the push button smoothly to the First Stop. 2. Aspiration: Immerse the pipette tip in the liquid. Allow the push button to move up smoothly to the Rest Position.
  • Page 15 www.medpip.com Before dispensing, carefully remove droplets on the outside of the tip with a lint-free cloth, being sure to stay clear of the tip opening. )figure 9( 4. Purge: Wait one second, then press the push button to the Second Stop. This purge stroke removes any remaining sample from the tip.
  • Page 16 3-2. Reverse Pipetting Rest Position First Stop Second Stop Figure 11. Reverse Pipetting 1. Preparation: Hold the pipette in a vertical position. Press the push button smoothly to the Second Stop. 2. Aspiration: Immerse the pipette tip in the liquid. Allow the push button to move up smoothly to the Rest Position.

  • Page 17: Proper Pipetting Advices

    www.medpip.com 4. Proper Pipetting Advices To achieve the highest accuracy while using P.I.P . pipette, follow the proper pipetting advices: Choose the right pipette. The nominal volume written on your pipette should fit the volume you plan to pipette. Use the appropriate pipette tip size from a high-quality manufacturer. (figure 12) Use single-use tips.
  • Page 18 To fit a disposable tip on P.I.P . pipettes: 1. Hold the micropipette in one hand. 2. Lower the pipette into the tip. 3. Use a slight twisting motion to seat the tip firmly on the tip holder of the pipette. (figure 13) To protect your pipette, avoid tapping the pipette onto the tip like a hammer.
  • Page 19 www.medpip.com Table 3. Appropriate Immersion Depth Volume Immersion Depth 0.1-1 μl 1-100 μl 2- 3 mm 101-1000 μl 2- 4 mm 1001-10,000 μl 3- 6 mm Choose the proper pipetting technique. )see page 12( Minimize pipette handling time and wear gloves to reduce body heat transfer to the pipette.
  • Page 20 4-1. Pipetting Influencing Parameters To find out about the factors affecting pipetting accuracy refer to table 4. Table 4. Pipetting Influencing Parameters Influencing Parameters Effects Corrective Actions Using appropriate and recommended 0.5% to 50% Leaky/Poorly seated pipette tips pipette tips )see page16( Reuse of pipette tips Up to 4% Using pipette tips only once...

  • Page 21: Cleaning And Maintenance

    www.medpip.com 5. Cleaning and Maintenance To clean the pipette: Never use corrosive cleaning agents or strong solvents. Check the compatibility with the materials used, before cleaning. Do not clean the device with sharp objects. External cleanings are recommended using normal cleaning agents such as water with mild detergents or 60%-70% ethanol.

  • Page 22: Decontamination

    6. Decontamination In the case decontamination is needed: 1. Only disassemble the tip ejector and shaft carefully. )figure 18, figure 19( The guarantee will be voided, if other parts of the pipette are disassembled. Piston and other components may be damaged if the shaft is carelessly disassembled.
  • Page 23 www.medpip.com To understand how to decontaminate P.I.P . pipette correctly, refer to table 5. Table 5. P.I.P . Pipette Decontamination Process Decontamination Contamination Causes Corrective Actions Techniques Disassemble the shaft and tip ejector of your pipette. Fully immerse the contaminated parts* into an ultrasonic bath with a detergent or cleaning solution Radioactive Compounds Detergent-Cleaning...

  • Page 24: Autoclaving

    7. Autoclaving Damage to device from incorrect handling. Do not use any additional disinfectants, chemicals, decontamination agents or Sodium Hypochlorite during autoclaving or UV Irradiation. Before autoclaving, take out the push button. )figure 20( Figure 20. Disassembling the Push Button The lower part of the pipette can be autoclaved in the assembled state.

  • Page 25: Pipette Service

    www.medpip.com 8. Pipette Service Notice that it is vital to contact P.I.P . company for any operational problems. To find out about P.I.P. pipette service intervals refer to table 6. Table 6. P.I.P. Pipette Service Intervals Frequency Action End-Users Daily Leak Test Weekly to up to every three Cleaning...
  • Page 26 Table 7. P.I.P . Pipette Troubleshooting Problem Cause Solution Pipette tip does not fit properly Use the appropriate tip size. )see page 16( onto the pipette tip cone. Liquid being pipetted is hot or Shorten the time cold. between pipetting steps. Liquid being pipetted is very use reverse mode pipetting.

  • Page 27: Shipment

    www.medpip.com 9. Shipment When sending a pipette for service, please notice the followings: 1. Contact us before sending the pipette back to the company: +98 21 88545922-9 2. In the case the pipette is contaminated, decontaminate the device in accordance with the instructions of table 5. )See page 22( CAUTION: Contact with a contaminated device may result in personnel severe diseases.

  • Page 28: Pipette Serial Number

    10. P.I.P. Pipette Serial Number Your P.I.P . pipette has been engraved with a Serial Number on the body of the pipette. It provides unique identification of your pipette. Registering the instrument via "my.medpip.com/en" will create an ownership record for the device.

  • Page 29: Pipette Warranty

    www.medpip.com 11. P.I.P. Pipette Warranty Pole Ideal Pars Co. warrants to the purchaser )Customer( of P.I.P . pipette )Product( that the instrument will be free of defects in workmanship and materials for a period of one year )Warranty Period( and also supports it for a period of 5-year after-sale service from the date of the purchase.
  • Page 30 Exceptions Any damage caused by Customer misuse, accident, fire or any disasters Any damage caused during shipment, transport and movement of the Product A vitiated Guarantee Card or Serial Number Component replacement by the Customer and any damage caused due to the replacement of wearing parts Service of the Product by a service supplier not authorized by P.I.P .

  • Page 31: Choosing The Proper Pipette

    www.medpip.com 12. Choosing the Propper Pipette Depending on your application, P.I.P . pipettes are available in a wide range of nominal volumes which offers you a lot of choices. In addition to the volumes named in the pipette Technical Data (table 8), you can order any desired volumes in accordance with your application needs.

  • Page 32: Pipette Technical Data

    GTIN Error Error Tips 0.0 2 μl 0. 01 μ l 0.5-1 0 μl 1 μ l 120260 6 26080 75030 27 0.0 3 μl 0.5-1 0 μl 0. 02 μ l 2 μ l 120261 6 26080 75030 10 0.0 4 μl...
  • Page 33 www.medpip.com Random Appropriate Product Systematic Volume GTIN Error Error Tips 0 .6 8 μl 1 0-100 μl 0 .1 8 μ l 8 0 μ l 1 2 0 2 7 9 6260807 503201 0 .7 0 μl 1 0-100 μl 0 .1 9 μ...
  • Page 36 Note...

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