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Summary of Contents for SEMC J1230
- Page 1 J1230 User Manual Simons Electron Microscopy Center (SEMC) at the New York Structural Biology Center (NYSBC)
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Page 2: Table Of Contents
Last updated on 9/20/19 Table of Contents J1230 Information Sheet J1230 User Guide Troubleshooting Alignments Microscope Recovery... -
Page 3: J1230 Information Sheet
Last updated on 9/20/19 J1230 Information Sheet ● Cs = 2.0 mm ● Apertures ○ Condenser = 300, 200, 100 um ○ Objective = 80, 50, 20 um ● Important buttons: ○ Use LOW MAG or MAG1 mode (not MAG2) ○... -
Page 4: J1230 User Guide
Last updated on 9/20/19 J1230 User Guide 1. Beginning of session: a. HT = 80 kV and “READY” b. Beam = OFF c. Microscope should have been left in SA mag range d. Objective aperture OUT e. Screen DOWN Camera at -25℃... - Page 5 Right click to open a terminal and type betaleginon c. Follow prompts to create a new session d. Choose “j1230 robot holder” as holder and enter a session description e. When prompted, connect client to j1230-cam1 Select camera client, click “+” button and okay...
- Page 6 Last updated on 9/20/19 10. Adjust preset intensity - this should be checked for every square used for imaging as stain thickness varies a. Send sq preset to scope b. With the flu screen down, adjust current density (on monitor) to be between 20 and 25 pA/cm2 using the brightness knob on the control panel *note: if you cannot see beam, lower mag and adjust beam shift first c.
- Page 7 Last updated on 9/20/19 Spc Pos… → Position → click center of stage map (make sure Point option is ON) Tilt/Rot selected → click center of stage map Retract holder g. Remove grid and store holder inside column...
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Page 8: Troubleshooting
● Exposure and Focus node should use “Image Beam Shift” to move to target ● Grid, Square and Subsquare node should use “Stage position” to move to target ● YOU MUST USE THE J1230 Raster app! STAFF USE ONLY Alignments Frequently you will need to adjust gun tilt and possibly gun shift after a vacuum crash. -
Page 9: Microscope Recovery
Last updated on 9/20/19 Microscope Recovery If the J1230 has experienced a vacuum crash upon insertion or retraction please complete the steps under MICROSCOPE RESTART. If the J1230 computer has frozen please complete the steps under COMPUTER RESTART. For any other microscope issues please consult with EMG staff before proceeding. - Page 10 Last updated on 9/20/19...
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