RainDance RainDrop Sense Operator's Manual
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RainDance RainDrop Sense Operator's Manual

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Summary of Contents for RainDance RainDrop Sense

  • Page 1 ® RainDrop Sense Operator’s Manual...
  • Page 2 You may make a reasonable number of copies of this manual for internal use only and only for use in connection with the RainDrop Sense Instrument. All such copies shall contain the copy- right info set forth on Page ii of this manual and this reproduction disclaimer. Except as explicitly provided by this section, neither this manual nor any part of it may be reproduced, photocopied, or electronically transmitted in any way without the advanced written permission of RainDance.

  • Page 3: Table Of Contents

    Space Requirements..................3-2 Gas Connectivity..................3-2 Connecting RainDrop Sense and Instrument Controller ........3-3 Connecting Cables to the RainDrop Sense Instrument ....... 3-3 Connecting Cables to the Instrument Controller.......... 3-4 Gas Pressure Quality ..................3-6 Starting Up the RainDrop Sense Instrument............. 3-6 Relocating RainDrop ..................
  • Page 4 RainDrop Sense Operator’s Manual Launching the Instrument Control Software (ICS) Chapter 4: Introduction ....................... 4-2 The Microsoft Windows 7 Login ................ 4-2 Starting the ICS Application ................4-2 ICS Login Levels..................4-3 Changing the ICS Password................ 4-5 The ICS Account Types................4-6 ICS Initialization ...................
  • Page 5 RainDance Support................... 7-2 RainDance Planned Maintenance..............7-2 Customer Maintenance ..................7-2 Restarting the IC Workstation..............7-2 Cleaning the RainDrop Sense ..............7-2 Refilling the Oil Reservoir.................. 7-3 Service and Diagnostic Requirements .............. 7-6 Powering Off the System .................. 7-6 Powering On or Restarting the System............. 7-6 Restoring the System After a Failure ..............

  • Page 6: Chapter 1: Foreword

    Where there is any conflict between the RainDance Software End User License Agreement included in the software, and the terms and conditions of sale, the order of precedence shall...

  • Page 7: Intended Use

    RainDrop Sense Operator’s Manual be as follows: (1) the RainDance Software End User License Agreement and (2) the terms and conditions. Intended Use For Research Use Only. Not for use in Diagnostic Procedures. RainDance Technologies RainDrop Sense is the detection instrument that enables identification and counting of target molecules in each picoliter droplet following PCR amplification.

  • Page 8: Reproduction Disclaimer

    You may make a reasonable number of copies of this manual for internal use only and only for use in connection with the RainDrop Sense Instrument. Except as provided by the previous sentence, neither this manual nor any part of it may be reproduced, photocopied, or electronically transmitted in any way without the advanced written permission of RainDance.

  • Page 9: Notes

    Notes are set apart from the body of the document with either the word Note or Important. They are meant to draw your attention to reminders and information that is important to successful operation of the RainDrop Sense Instrument. Note: A Note indicates a useful piece of information.

  • Page 10: Cautions

    Caution: Do not operate the System in a manner other than specified in this manual. Doing so could damage the instrument or produce erroneous results. Caution: RainDance service representatives are trained in the safe operation of the RainDrop Sense Instrument, including accessing those areas reserved for trained service representatives.

  • Page 11: Laser Safety Warnings

    RainDrop Sense Operator’s Manual Laser Safety Warnings The RainDrop Sense, classified as a Class 2 System, contains a Class 3B laser and Class 2 barcode laser. Avoid direct exposure to the Class 3B laser radiation when the unit is open and interlocks are defeated.

  • Page 12: Safety Information

    RainDrop Sense Operator’s Manual Safety Information Electrical Safety Warnings Warning: This equipment is operated with hazardous voltages that can shock, burn, or cause death. To reduce the possibility of electrical shock, do not remove any fixed panels. Ensure that all service to the system is performed only by qualified RainDance service personnel.

  • Page 13: Mechanical Safety

    RainDrop Sense product label. Figure 1-2: RainDrop Sense Product Label Safety Interlock The RainDrop Sense is equipped with an automatic safety interlock, which prevents it from operating unless its door is properly closed. Packing and Transport RainDance provides initial shipping and installation. If you are considering moving the...

  • Page 14: Introduction

    2-4 RainDrop Carrier and Drive Oils page 2-4 RainDrop Sense Barcode Reader page 2-5 RainDrop Sense Chip page 2-6 Handling the Sense Chip page 2-6 RainDrop Sense Chip Compression Plate page 2-7 Specifications page 2-8 LCN 50-04344 Rev. D...

  • Page 15: System Overview

    RainDrop Sense Operator’s Manual ® RainDrop System Overview ® The RainDance RainDrop System comprises two instruments: the RainDance Source and the RainDrop Sense. RainDrop System Major Components RainDance Source Instrument  RainDrop Sense Instrument  Two Instrument Control (IC) Workstations (computers), one for each instrument ...

  • Page 16: Raindrop Sense Major Components

    RainDrop Sense Operator’s Manual RainDrop Sense Major Components Figure 2-2 shows the major components of RainDrop Sense instrument. Instrument control workstation monitor RainDrop Sense instrument Figure 2-2: RainDrop Sense Major Components LCN 50-04344 Rev. D...

  • Page 17: Raindrop Sense Layout

    Precise control of carrier and drive oil flow rates on the Sense instrument allows for control of droplet spacing. Use only RainDance- supplied oils to avoid damage to the RainDrop System. The carrier oil and drive oil ports can be filled with the oil provided in the Carrier Oil Syringe.

  • Page 18: Raindrop Sense Barcode Reader

    Items bearing a user-generated barcode for sample tracking (within the limits of  supported barcode formats) Figure 2-4: RainDrop Sense Barcode Reader Scanning Barcodes Scan items as follows: 1. Click Scan in the ICS software. The red scanner light pulses for 10 seconds.

  • Page 19: Raindrop Sense Chip

    RainDrop Sense Operator’s Manual RainDrop Sense Chip The RainDrop Sense Chip is placed into the instrument on top of the PCR tube strip. As part of the Sense process, the chip punctures the tube strip caps and transfers the emulsified sample through its lanes where the Sense instrument identifies and counts the droplets.

  • Page 20: Raindrop Sense Chip Compression Plate

    Sense Instrument. Position the plate over the Sense chip prior to start of the run. When the plate is properly positioned, its RainDance logo should be facing you, the plate should be flat, and it should not be movable left to right or front to back.

  • Page 21: Specifications

    RainDrop Sense Operator’s Manual Specifications Table 2-1 shows the specifications for the RainDrop Sense instrument. Table 2-2 shows the specifications for the Instrument Control (IC) Workstation. Table 2-1: RainDrop Sense Hardware Specifications Requirement or Specification Description Width 11 inches (27.9 cm) Depth 17 inches (43.2 cm)
  • Page 22 The Instrument Control Software has the ability to email notifications to customers if it is networked. In addition, RainDance has software that allows us, with customer permission, to access the computers to look at log files to assist in troubleshooting and to assess instrument performance.
  • Page 23 101-81 kPa (1010-810 mbar) or sea level to 1800 m Safety Compliance IEC 61010-1:2010, the international safety standard for laboratory equipment, including USA (UL), Canadian (CSA), and European (CENELEC/EN) differences Data Backup RainDance suggests using a standard backup procedure for any computer system. LCN 50-04344 Rev. D 2-10...

  • Page 24: Chapter 3: Installation And Setup

    Gas Pressure Quality page 3-7 Starting Up the RainDrop Sense Instrument page 3-8 Relocating RainDrop page 3-9 Important: Only trained and certified RainDance Service Representatives may remove the ® RainDrop Sense instrument from its packaging and install it. LCN 50-04344 Rev. D...

  • Page 25: Space Requirements

    Minimum Width 29 inches (74 cm) Note: Refer to the Pre-Site Guide for any additional installation details. Caution: The RainDrop Sense instrument is sensitive to vibration and should be placed on a bench top that is free from vibration. Gas Connectivity Gas source should be located in proximity to the instrument because the gas supply connection is located on the back of the instrument.

  • Page 26: Connecting Raindrop Sense And Instrument Controller

    RainDrop Sense Operator’s Manual Connecting RainDrop Sense and Instrument Controller Before powering on the RainDrop Sense instrument and its IC Workstation, make sure all cables are properly connected as described in “Connecting Cables to the RainDrop Sense Instrument” on page 3-3 “Connecting Cables to the Instrument Controller”...

  • Page 27: Connecting Cables To The Instrument Controller

    Important: The Detachable Main AC Power Cable for North America or other countries with 120 VAC 50/60 Hz supply voltage provided for the RainDrop Sense instrument is a 3-wire cord, 18 AWG, 10 A, 125 VAC. Never substitute a Detachable Main AC Power Cable with a lower current or voltage rating.
  • Page 28 RainDrop Sense Operator’s Manual HDMI port that connects to the monitor USB port that connects to the left output from the Sense USB port that connects to the right output from the Sense Figure 3-2: Cable Connections to the IC Workstation The left USB/output connects to the USB port furthest from the gas inlet and the right USB/output connects to the other available USB port.
  • Page 29 RainDrop Sense Operator’s Manual USB receiver Figure 3-3: USB Receiver 1. With the IC Workstation turned off, plug the USB receiver for the keyboard and mouse into the IC Workstation. 2. Plug the right USB output from the Sense instrument into the right USB in Figure 3-2.

  • Page 30: Gas Pressure Quality

    RainDrop Sense Operator’s Manual Gas Pressure Quality You may use compressed argon or nitrogen. Ensure that the compressed gas system you use meets the following standards. The input pressure must be regulated to a value, at the system, within the range of 90- 120 psi.

  • Page 31: Starting Up The Raindrop Sense Instrument

    Starting Up the RainDrop Sense Instrument Use the following startup sequence for the RainDrop Sense instrument: 1. With the RainDrop Sense and IC Workstation turned off, ensure that all the cables have been connected properly as described in “Connecting Cables to the RainDrop Sense Instrument”...

  • Page 32: Relocating Raindrop

    Instrument is disconnected from the ICS. Relocating RainDrop Caution: The RainDrop Sense instrument weighs approximately 65 lbs (29 kg). To avoid injuries and damage to the instrument, do not move it without assistance from RainDance Technologies. If you want to move your instrument either within your laboratory or to another location, contact RainDance Technologies.

  • Page 33: Chapter 4: Launching The Instrument Control Software (Ics)

    CHAPTER 4 Chapter 1: Launching the Instrument Control Chapter 4: Software (ICS) This chapter covers the following topics: Introduction page 4-2 The Microsoft Windows 7 Login page 4-2 Starting the ICS Application page 4-2 ICS Login Levels page 4-3 Changing the ICS Password page 4-5 The ICS Account Types page 4-6...

  • Page 34: Introduction

    ICS failures. The default login is an administrative account. The system differentiates between upper and lower case characters. Note: Do not use the RainDance Support Windows Logon. This will result in ICS failure. Starting the ICS Application The startup sequence works as follows: 1.

  • Page 35: Ics Login Levels

    The ICS application supports two levels of access to the ICS program: User and Administrator. The ICS application is configured with two default login IDs: one for User and one for Administrator. RainDance recommends that you change the default password on each of these accounts after initial use.
  • Page 36 RainDrop Sense Operator’s Manual Caution: After three failed attempts to login with the wrong password, the ICS application locks the account. You will not be able to login to your account. If this happens, you must contact someone in your facility with an Administrator account who can either unlock or reset your password.

  • Page 37: Changing The Ics Password

    RainDrop Sense Operator’s Manual Changing the ICS Password This section describes how to change an ICS password. RainDance recommends that you change the default User and Administrator passwords after initial use. 1. Select Change Password from the Home menu. The Change Password dialog opens (Figure 4-3).

  • Page 38: The Ics Account Types

    RainDrop Sense Operator’s Manual The ICS Account Types Your account type defines the features you have access to (User and Administrator) within the ICS application. Table 4-2: ICS Account Type Feature User Administrator Change Passwords Export Instrument Files View Runs...

  • Page 39: Ics Initialization

    RainDrop Sense Operator’s Manual ICS Initialization When you login to the instrument, the Initialization screen appears (see Figure 4-4). Figure 4-4: Initialization Screen From the Initialization screen, click Start Initialization to initialize the system. A progress bar shows the progress of the initialization.
  • Page 40 Click OK and contact RainDance Support. See “RainDance Support” on page 1-3. This error is often the result of starting ICS right after you turn on the instrument. RainDance recommends the following steps: 1. Exit the ICS. 2. Turn the instrument off....
  • Page 41 RainDrop Sense Operator’s Manual If initialization is successful, the ICS home page is displayed (see Figure 4-7). Note: All menu items are inactive until the initialization is finished. Figure 4-7: Home Page From the home page, you can Setup a Run (see...

  • Page 42: Status Indicators

    RainDrop Sense Operator’s Manual Status Indicators A common set of status indicators appears throughout the system indicating the status of an item or process being performed. In general, the green check mark ( ) indicator means that everything is working as expected and you can proceed. The orange exclamation point (!) indicator is a warning that means that you can continue, but that some optional fields are blank.

  • Page 43: Using Tabs In The Ics

    RainDrop Sense Operator’s Manual Using Tabs in the ICS There are two tabs in the ICS that appear after you click Setup a Run: Run Data and Run Info. To perform a run, you will need to complete some of the fields on each tab.

  • Page 44: Navigating Through The Tabs

    RainDrop Sense Operator’s Manual To prepare for a run on the Sense instrument, you must complete the following fields in the Sense ICS: Run Data Tab:  Run Name  Sense Chip barcode lot and serial number  At least one selected lane (indicated by a green check mark) ...

  • Page 45: Completing A Process

    When you are satisfied that the steps for the process are complete, click Start Run on the bottom right side of the Run Data tab. This starts the process on the RainDrop Sense instrument. Figure 4-10: Start Run Button Using Scan Buttons The ICS allows you to scan barcodes to enter information into various text fields.

  • Page 46: Alarms, Log, And Status Tabs

    RainDrop Sense Operator’s Manual Alarms, Log, and Status Tabs The Alarms, Log, and Status tabs are displayed on the bottom of the screen (see Figure 4- 11). Each of these tabs is described below. Figure 4-11: Example of Screen Showing Alarms, Log, and Status Tab at Bottom The Alarms tab is displayed by default.

  • Page 47: Chapter 5: Performing A Sense Run

    CHAPTER 5 Chapter 1: Performing a Sense Run Chapter 5: This chapter covers the following topics: Introduction page 5-2 Gathering Supplies page 5-2 Starting a Sense Run page 5-3 Completing the ICS Setup page 5-10 Checking the Status Indicators page 5-10 Completing the Run Data Tab page 5-12 Completing the Run Info Tab...

  • Page 48: Introduction

    “RainDrop® Consumables and Equipment” on page A-1. This chapter describes how to start a run on the RainDrop Sense instrument. Some of the steps involve preparing the physical components of the system and some of them take place within the RainDrop ICS.

  • Page 49: Starting A Sense Run

    It also assumes that you have completed thermal cycling the sample. It shows you how to start a run on the RainDrop Sense instrument. 1. Launch the ICS software for the Sense instrument and log in.
  • Page 50 RainDrop Sense Operator’s Manual Note: If initialization fails, the ICS returns to the Initialization screen after about fifteen minutes. Contact RainDance Support for assistance. See “RainDance Support” on page 1-3. 3. Click Setup a Run. Figure 5-3: Instrument Status and Setup a Run Screen...
  • Page 51 RainDrop Sense Operator’s Manual 4. Select the Run Info tab. Figure 5-4: Run Info Tab 5. On the Run Info tab, click Open Door. The door on the Sense instrument unlocks allowing you to lift it to the upright, open position.
  • Page 52 RainDrop Sense Operator’s Manual A bump in the cap Figure 5-5: Improperly Covered PCR Tube Strip 7. Lift the door to the fully open position. LCN 50-04344 Rev. D...
  • Page 53 RainDrop Sense Operator’s Manual Figure 5-6: Sense Door in Fully Open Position LCN 50-04344 Rev. D...
  • Page 54 Figure 5-7: Insert a Tube Strip 9. Remove a Sense Chip from its packaging; for more information on the chip, see “RainDrop Sense Chip” on page 2-6. Handle the Sense Chip as follows: With gloved hands, grasp the chip by its sides.
  • Page 55 Figure 5-9. When the plate is properly positioned, its RainDance logo should be facing you, the plate should be flat, and it should not be movable left to right or front to back. Figure 5-9: Placing the Chip Compression Plate After you insert the Chip Compression Plate, you are done setting up the physical components.

  • Page 56: Completing The Ics Setup

    RainDrop Sense Operator’s Manual Completing the ICS Setup Before starting a run on the Sense instrument, you must complete a number of setup steps. The steps ensure that the instrument is ready for operation and provide data about the run.
  • Page 57 RainDrop Sense Operator’s Manual Table 5-1: System Status Indicators Chip Indicates the presence of the Sense Chip. When the Sense Chip is present and inserted properly, the ready indicator is displayed.  Inserted Note: You must scan the chip prior to use; otherwise, the instrument will not detect it.

  • Page 58: Completing The Run Data Tab

    RainDrop Sense Operator’s Manual Completing the Run Data Tab The Run Data tab opens after you click Start Run on the startup screen and contains the details about the contents of the run. You can enter this information manually or you can import it from a file you saved on the Source instrument.
  • Page 59 RainDrop Sense Operator’s Manual Table 5-2: Run Data Fields Field Name Meaning Run Name The name of the run. Run name is required and can contain up to 20 characters. This is required. Lanes A - H The identifiers for each tube (or lane) on the chip. These fields are not required.
  • Page 60 RainDrop Sense Operator’s Manual Scan the barcode from a printout. To do so, place the printout in front of the  barcode reader and click Scan next to the correct lane. When you enter the name using the scanner, the field limits entry to 96 characters.

  • Page 61: Completing The Run Info Tab

    RainDrop Sense Operator’s Manual Completing the Run Info Tab The fields on the Run Info tab contain information about the run. The only fields on this tab that are required are the Carrier Oil and Drive Oil fields and only if the instrument is out of one of these types of oil.

  • Page 62: Completing The Run Tabs Using The Import Function

    4. Complete any other of the optional fields, as needed. Using Email Notification The RainDrop Sense instrument is located in a lab space which may be a distance from your desk or usual work area. Email Notification allows you to receive messages when a run is finished, so that you do not have to continually check the instrument.
  • Page 63 The name the user entered in the Run Name field prior to beginning the run. If the user does not enter a Run Name, this lists a name provided by the RainDrop Sense instrument. Description The comments entered by the user at run time, if any...

  • Page 64: Performing The Run

    RainDrop Sense Operator’s Manual Performing the Run After you have entered the required run data, you are ready to begin the run. 1. Click the Start Run button. The screen displays information about the Sense run in progress as shown in the following image.
  • Page 65 RainDrop Sense Operator’s Manual The ICS displays the Run Complete screen. Figure 5-14: Sense Run Complete Screen 5. Click View Run Report to review the run data. 6. Click Save and Close. ICS saves the data from the run in a file in FCS format, which can be read by the RainDrop Analyst software.

  • Page 66: Canceling A Run

    RainDrop Sense Operator’s Manual Canceling a Run If you want to stop a run after you have started filling out the Run Data tab, but before clicking Start Run, click Cancel. This brings you back to the Instrument Status and Setup a Run...

  • Page 67: Handling Data

    Handling Data The data from Sense runs is collected on the hard drive of the ICS Workstation. Files are stored in folders on the D drive named for their runs within a RainDance folder as follows: D:\RainDance\RunLogs\<RunID> The <RunID> is displayed in the upper right corner of the ICS screen on both the Run Data tab and the Run Info tab.

  • Page 68: Exporting Data

    RainDrop Sense Operator’s Manual Exporting Data The Export Data function allows you to search for previous runs and to save that run data to either the same or a new location. This allows you to move your FCS files from one location to another to make them available for use by other applications such as analysis software.

  • Page 69: Moving Data Files

    You can use the Export process described above to locate your FCS files in a location that is accessible by your analysis software. In addition, RainDance offers the following instructions for moving your files when disk space becomes an issue on the ICS Workstation.
  • Page 70 RainDrop Sense Operator’s Manual 2. Open the RainDance folder on the D drive. 3. Open the Run folder whose data you want to copy. 4. Click the Type column to sort the data files by type. The only files you need to move to the computer running RainDrop Analyst II are the FCS files.

  • Page 71: Chapter 6: Managing The System

    CHAPTER 6 Chapter 1: Managing the System Chapter 6: This chapter covers the following topics: Introduction page 6-2 Managing Users page 6-2 Adding a New User page 6-3 Editing a User page 6-5 Deleting a User page 6-7 Resetting Password or Unlocking an Account page 6-7 Viewing Past Runs page 6-8...

  • Page 72: Introduction

    RainDrop Sense Operator’s Manual Introduction You must have a username and password before you can login to the ICS application. There are two types of user accounts: Administrator and User. Chapter 4 provides detailed information on logins and account types.

  • Page 73: Adding A New User

    RainDrop Sense Operator’s Manual Adding a New User To add a new user: 1. From the Actions menu, select Add New User (see Figure 6-1). The Add New User dialog opens (see Figure 6-2). Figure 6-2: Add New User Dialog 2.
  • Page 74 RainDrop Sense Operator’s Manual Table 6-1: Add New User Dialog Field or Table Element Description User Name Specifies the name you use to login. Case sensitive. Required. Password Specifies the password; any alphanumeric combination is acceptable. Case sensitive. Required. First Name, Last Name Specifies the user's first and last names.

  • Page 75: Editing A User

    RainDrop Sense Operator’s Manual Editing a User The Edit Users dialog allows a user with administrator privileges to edit the information for an existing user or remove an existing user from the database. An administrator can also add a new user from this dialog.
  • Page 76 RainDrop Sense Operator’s Manual 2. To edit the data for an existing user, click Edit in the row for that user. The Edit User Information dialog opens for the selected user (see Figure 6-4). Figure 6-4: Edit User Dialog Box 3.

  • Page 77: Deleting A User

    RainDrop Sense Operator’s Manual Deleting a User To delete a user: 1. Select Edit Users from the Actions menu (see Figure 6-1). The Edit Users dialog opens as in Figure 6-3. 2. Click Remove in the same row as the user to be deleted. The system asks you to confirm that you want to delete the user.

  • Page 78: Viewing Past Runs

    RainDrop Sense Operator’s Manual Viewing Past Runs This section describes how to view previous runs. It also describes how to generate and view reports and how to export run results. Viewing Runs Certain run parameters are saved by the ICS application. The ICS application lets you search for and view past run parameters by entering a specific Start Date and an End Date (inclusive) on which the run was completed.
  • Page 79 RainDrop Sense Operator’s Manual 3. Clicking or selecting a row in the list of runs opens the report for that run. This may take 2-5 seconds. Figure 6-6: Example of a Sense Run Report Reports are divided into three sections. The first section contains: Run Name ...
  • Page 80 RainDrop Sense Operator’s Manual 4. From the menu bar (see Figure 6-7) you can print the report or export it to various formats, such as a PDF file or an Excel file. Figure 6-7: Report Menu 5. When you are done viewing the report, close it by clicking on the Close box in the upper right corner of the screen.

  • Page 81: Chapter 7: Support, Customer Maintenance, And Troubleshooting

    7-2 RainDance Planned Maintenance page 7-2 Customer Maintenance page 7-2 Restarting the IC Workstation page 7-2 Cleaning the RainDrop Sense page 7-2 Refilling the Oil Reservoir page 7-3 Service and Diagnostic Requirements page 7-6 Powering Off the System page 7-6...

  • Page 82: Raindance Support

    RainDance Support personnel. A number of PMs are conducted as part of the service contract agreement. Contact RainDance Support for a recommended PM schedule. Customer Maintenance This section lists the steps you should follow for proper maintenance of the RainDrop Sense system. Restarting the IC Workstation Good operating practice for any Windows operating system includes restarting the operating system on a weekly basis.

  • Page 83: Refilling The Oil Reservoir

    Refilling the Oil Reservoir The oil reservoir on the RainDrop Sense instrument must be refilled from time to time. You will know that it is time to refill the reservoir when the oil light on the front of the instrument glows red.
  • Page 84 RainDrop Sense Operator’s Manual To refill the oil reservoir: 1. Obtain a pre-measured syringe of oil (P/N 30-07117). 2. Scan the barcode on the oil syringe. Refer to “Scanning Barcodes” on page 2-5 more information on how to scan. 3. Remove the cap from the tip of the syringe.
  • Page 85 RainDrop Sense Operator’s Manual 6. Place the tip of the syringe over the fill port and begin to turn the entire syringe clockwise. The syringe screws onto the fill port to create a tight fit. Figure 7-3: Syringe Connected to the Fill Port 7.

  • Page 86: Service And Diagnostic Requirements

    There are no user serviceable parts inside the RainDrop instrument. For safety  reasons, those components have limited access. All technical maintenance or service is provided by RainDance Support.  Powering Off the System Follow this sequence to turn the system off: 1.

  • Page 87: Support Agreement Information

    Support Agreement link. Moving the System Warning: The RainDrop Sense instrument weighs approximately 65 lbs (29 kg). To avoid injury and damage to the instruments, do not move them without assistance from RainDance Technologies. To move the instrument to another location, contact RainDance Support.

  • Page 88: Troubleshooting

    RainDrop Sense Operator’s Manual Troubleshooting The following table lists some problems that you may observe while working with the RainDrop Sense instrument. The table is divided into three columns as follows: Observed Problem  Possible Cause  Corrective Actions and Comments ...
  • Page 89 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument The ICS does not display You have under-filled one The sample in that lane is droplets during a run or or more sample wells. This lost. Deselect the displays an unstable pattern of...
  • Page 90 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument One of the lanes does not Emulsion is not detected The sample is lost. In display any droplets. In because the emulsion some cases, you may be addition, the run may stop sensor is tripped.
  • Page 91 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument I tried to fill the oil reservoir It is possible to overfill the For this reason, do not before the oil light came on. oil reservoir and to spill oil...
  • Page 92 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument The Alarms tab or Log tab A known error has Follow the instructions displays an error message. The occurred. The ICS may provided by the error ICS displays an error message suggest a way for you to message.
  • Page 93 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument Some of the expected lanes You did not select the The sample may be were not processed. The lanes on the Run Data tab recoverable, but you sample remains in the Sense...
  • Page 94 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument I am not receiving emails, even You have entered an If you are not receiving though I have entered an email invalid email address. The email messages at the end address.
  • Page 95 RainDrop Sense Operator’s Manual Table 7-1: Troubleshooting the RainDrop Sense Instrument The sample has evaporated. The tube strip cap was not Ensure that the tube strip The sample volume in one or seated properly or the tube cap is seated properly. It...

  • Page 96: Chapter 8: Waste Management

    CHAPTER 8 Chapter 1: Waste Management Chapter 8: This chapter covers the following topics: Introduction and Disclaimer page 8-2 Classification of Waste page 8-2 Anticipated Typical Waste Stream Composition page 8-3 Recommended Waste Stream Disposal page 8-4 LCN 50-04344 Rev. D...

  • Page 97: Introduction And Disclaimer

    RainDrop Sense Operator’s Manual Introduction and Disclaimer ® The waste stream from the RainDrop platform includes fluorocarbon oil-emulsified aqueous samples and wetted solid materials (PCR tubes, chips, etc.). The following guidelines are intended to assist the customer in communicating with Environmental Health and Safety staff and/or commercial waste disposal service providers.

  • Page 98: Anticipated Typical Waste Stream Composition

    RainDrop Sense Operator’s Manual Anticipated Typical Waste Stream Composition During normal workflow operation, for every 96 samples run on the RainDrop Sense system, approximately 570 grams (20.11 oz) of waste is generated. The following approximate composition of the waste stream is expected if all consumables and waste reagents are...

  • Page 99: Recommended Waste Stream Disposal

    RainDrop Sense Operator’s Manual Figure 8-2: Spent Sense Chip with Tube Strip Attached Recommended Waste Stream Disposal Depending on applicable environmental laws and regulations, the waste stream, which  contains fluorinated chemicals, should be incinerated in an industrial or commercial facility in the presence of a combustible material.

  • Page 100: Chapter 9: Customer Care Information

    CHAPTER 9 Chapter 1: Customer Care Information Chapter 9: This chapter covers the following topics: General Care Information page 9-2 Consumables page 9-2 LCN 50-04344 Rev. D...

  • Page 101: General Care Information

    Order consumables from RainDance or your RainDance-authorized distributor. Use only RainDance products or RainDance-approved products in your RainDrop Sense instrument. RainDance shall not be liable for damage to or malfunction of the system that it deems was caused by the use of unauthorized materials.

  • Page 102: Chapter 10: Regulatory Information

    CHAPTER 10 Chapter 1: Regulatory Information Chapter 10: This chapter covers the following topics: Sense Instrument Declaration of Conformity page 10-2 For Research Use Only. Not for use in diagnostic procedures. LCN 50-04344 Rev. D 10-1...

  • Page 103: Sense Instrument Declaration Of Conformity

    RainDrop Sense Operator’s Manual Sense Instrument Declaration of Conformity Figure 10-1: Sense Instrument Declaration of Conformity LCN 50-04344 Rev. D 10-2...
  • Page 104 RainDrop Sense Operator’s Manual Figure 10-1 (continued): Sense Instrument Declaration of Conformity LCN 50-04344 Rev. D 10-3...
  • Page 105 RainDrop Sense Operator’s Manual Figure 10-1 (continued): Sense Instrument Declaration of Conformity LCN 50-04344 Rev. D 10-4...

  • Page 106: Chapter 11: Symbol Glossary

    CHAPTER 11 Chapter 1: Symbol Glossary Chapter 11: The symbols in the following table are used on the RainDrop Sense instrument. Table 11-1: Symbol Glossary Symbol Name Symbol Illustration Definition AC symbol Alternating current Ground symbol in circle Protected earth (ground) “|”...
  • Page 107 RainDrop System. Table A-1 provides a list of consumables, their associated part numbers and other ordering information. Caution: Where RainDance part numbers are indicated, use only RainDance- specified consumables to avoid damage to the instrument. LCN 50-04344 Rev. D...
  • Page 108 Includes the following: RainDance P/N 30-06085 (carton of 12 Source  Chips plus 13 PCR Tube Strip Caps) RainDance P/N 30-06086 (carton of 12 Sense Chips  plus 13 High Speed Tube Strip Caps) RainDance P/N 30-07117 Carrier Oil Syringe (3) ...
  • Page 109 RainDrop Sense Operator’s Manual Table A-1: Part Numbers for Customer-Supplied Consumables Adaptor to attach 4 mm O.D. tubing to male ¼ inch In the US: http:// adaptor on regulator www.coastpneumatics.com/ KQF04-02.html 4 mm in-line shut off valves In the US: http://www.mcmaster.com/...
  • Page 110 LCN 50-04344 Rev. D Date of Issue: February 2018 RainDance Technologies, Inc. 749 Middlesex Turnpike Billerica, MA 01821 Fax: (978) 528-6090 (support) Web: www.raindancetech.com/support Email: customercare@raindancetech.com Copyright © 2018 RainDance Technologies, Inc. All rights reserved.

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