Related Manuals for SPEX NanoSNAP
Summary of Contents for SPEX NanoSNAP
- Page 1 Operating Manual Spex NanoSNAP™ Nano Spectrophotometer for Nucleic Acids and Proteins Measurement...
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Page 2: Table Of Contents
Operating Manual Contents 1.0 Safety Precautions ........4 Intended Use . - Page 3 Operating Manual 9.0 System Setting ........38 10.0 History Information .
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Page 4: Safety Precautions
Operating Manual Safety Precautions Before using the Spex NanoSNAP microvolume spectrophotometer for the first time, please read this entire Operating Manual carefully. To guarantee problem-free, safe operation of the Spex NanoSNAP, it is essential to observe the following section. Intended Use This instrument is intended to be used by trained personnel to perform solution analysis. - Page 5 Operating Manual 1.2.3 Electrical Installation THIS INSTRUMENT MUST BE GROUNDED The appropriate power supply and cord combination should be connected to the equipment BEFORE connection to the mains supply. Before connection, please ensure that the electrical supply is suitable for the power requirements below: Current Current Voltage...
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Page 6: Chemical Waste Safety
Operating Manual 1.2.4 Cleaning, Decontaminating and Servicing the Instrument Before using a cleaning or decontamination method other than those recommended by the manufacturer, verify with the manufacturer that the proposed method will not damage the equipment. Switch off and unplug the device before cleaning, servicing or replacing the fuses. Repairs should be carried out by authorized service personnel only. -
Page 7: General Description
Operating Manual 2.0 General Description The Spex NanoSNAP is a fast, microvolume spectrophotometer that provides accurate and intuitive operational experience in only 3 seconds. It enables the measuring of samples from 190 to 1,000 nm, a broad range which offers flexibility for experiment. With the replaceable sample window, users don’t have to worry about residues. - Page 8 Quartz glass with nano hydrophobic coating layer. It has an indicating sample adding Sample Window design. It protects optical fibers and also reduces contamination. Light path of Spex NanoSNAP can be selected manually by pathlength selector Pathlength Selector according to the absorbance (concentration) range difference.
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Page 9: Getting Started
Indicates the model name, serial number, power specification, and other important Product Label information. 3.0 Getting Started 3.1 Unpacking Once you open the Spex NanoSNAP package, confirm that all of the following items are included: • Spex NanoSNAP spectrophotometer • Quick-Start Guide •... -
Page 10: Initial Operation
“Spex NanoSNAP” title will be displayed. Please DO NOT open the detection arm until system diagnosis is complete. Tap on the “Spex NanoSNAP” title to log into the Main Screen and start your operation. Tap on the Spex NanoSNAP title again on the Main Screen to log out. -
Page 11: Detection Arm Opening/Closing
Operating Manual 3.4 Detecting Arm Opening/Closing To open the arm, hold the detection arm and lift to the end as shown in Figure 5. Figure 5. Opening the arm To close the detection arm, hold the edge of the arm and let the detection arm down gently to the correct position as shown in Figure 6. -
Page 12: Adjusting The Pathlength Selector
The pathlength of the Spex NanoSNAP is not automatically selected but selected manually. The metal light path selector shown down below in Table 5 is the manual pathlength selector of the Spex NanoSNAP. The measurement range of 0.5 mm pathlength is 0.04 to 30 Abs, and the range of 0.05 mm is 20 to 400 Abs. - Page 13 Operating Manual Table 7. Function icons. Icon Function Description Blank To establish Blank data Auto Run: On The instruction of Auto Run function is on Auto Run: Off The instruction of Auto Run function is off Measure To do sample measurement or to do standard measurement To delete sample measurement data Delete Note: Standard measurement data cannot be deleted, but can be...
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Page 14: Application: Nucleic Acid
Operating Manual 4.0 Application: Nucleic Acid This application will measure the samples absorbance value at 260 nm, which is the peak of nucleic acid absorbing UV light, to calculate the concentration. The unit is ng/µL. The purity of nucleic acid samples can be estimated by two absorbance ratios, A260/280 and A260/230. -
Page 15: Protocol Operation
Operating Manual 4.1.2 Table Tab Page The table tab page will show all the data results. If the user needs to know more details of one data item, tap the check box column to select the data. The detail will be shown on the Data page and the Graph page. Figure 10. -
Page 16: Calculation
0.5 mm is from 2 to 1,500 ng/µL, and 0.05 mm is from 1,000 to 20,000 ng/µL. All method ranges are shown on the Spex NanoSNAP screen. Add appropriate solution of at least 1 µL and tap on the Blank icon to establish blank data. -
Page 17: Application: Protein A280
Operating Manual 5. Application: Protein A280 This application will measure the samples absorbance value at 280 nm, which is the peak of purified protein absorbing UV light, to calculate the concentration. The unit of protein concentration is mg/mL. The purity of homogeneous protein can be estimated by absorbance ratios of A260/280. -
Page 18: Protocol Operation
Operating Manual 5.1.2 Table Tab Page The table tab page will show all the data results. If the user needs to know more details of one data item, tap the check box column to select the data. The detail will be shown on the Data page and the Graph page. Figure 13. -
Page 19: Calculation
0.5 mm is from 0.06 to 45 mg/mL, and 0.05 mm is from 30 to 600 mg/mL. All the method ranges are shown on the Spex NanoSNAP screen. Add appropriate solution of at least 1 µL and tap on the Blank icon to establish blank data. -
Page 20: Application: Protein Assay
Operating Manual 6. Application: Protein Assay This application will measure homogeneous protein absorbance value at different wavelengths, according to different protein assay reagents. The unit of this protocol is mg/mL. 6.1 Overview of Screen Features The screen of protein assay protocol can be separated into 3 parts: information tab bar, information report area, and functions icons. - Page 21 Operating Manual 6.1.2 Standard Tab Page The standard tab page (Figure 16) is the page to measure standard samples absorbance at specific wavelength and establish standard curve. It only shows standard data result. The features are shown below (Table 13). Information tabs Sample data information Curve type selection...
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Page 22: Protocol Operation
Operating Manual Tap the Sample column to change the sample name. The Blank can also be renamed, but this is NOT suggested. You need to measure the Blank again if the original blank has been renamed. Note: Only one data item can be checked at one time. 6.1.4 Graph Tab Page In the protein assay protocol, there are 2 kinds of graphs: the standard curve graph and sample absorbance graph. - Page 23 Operating Manual Select the repetition frequency you need in the Repetition bar. Tap on a cell in the mg/mL column to enter the concentration of your standard sample. Tap on a cell in the Abs column and select the Measure icon to establish standard data. If the repetition frequency is higher than 1, it will automatically jump to the next cell.
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Page 24: Calculation
Operating Manual Add your sample of at least 1 µL and select the Measure icon to enter sample measurement. After the experiment, clean the sample window and cover on the detection arm with a lint-free wipe. Use deionized water, ethanol or isopropanol if needed. 15. -
Page 25: Application: Od 600
Operating Manual 7. Application: OD 600 This application will measure microbial cell samples absorbance at 600 nm which can be used for monitoring the samples growth rate. The range of the light pathlength is shown in the unit of absorbance. The absorbance also can be calculated to concentration with a conversion factor, which is an optional function in this protocol. -
Page 26: Protocol Operation
Operating Manual 7.1.2 Table Tab Page The table tab page will show all the data results. Is the user needs to know more details of one data item, tap the check box column to select the data. The detail will be shown on the Data page and the Graph page. Figure 20. -
Page 27: Calculation
Open the detection arm and turn the pathlength selector to the appropriate position. The range 0.5 mm is from 0.04 to 30 Abs, and 0.05 mm is from 20 to 400 Abs. All the ranges are shown on the Spex NanoSNAP screen. -
Page 28: Application: More Assays
Operating Manual 8. Application: More Assays In More Assays, the Spex NanoSNAP has 3 customized applications for users to establish self-defined protocols. 8.1 Factor Method In this application, users can measure samples at their selected wavelength. They can also insert self-defined units and the conversion factor (optional). - Page 29 Operating Manual Information tabs Insert sample name Sample data information Selected pathlength Function icons Figure 23. Data Tab Page On the data tab page of Factor Method protocol (Figure 23), the data information parts have the features below (Table 16). The function icons will be activated after finishing the protocol setting in the Custom Setting tab page. Table 16.
- Page 30 Operating Manual Tap the Sample column to change the sample name. The Blank can also be renamed, but this is NOT suggested. You need to measure the Blank again if the original blank data has been renamed. Note: Only one data item can be checked at one time. The graph of measuring samples can be enlarged or minimized.
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Page 31: Std. Curve Method
Operating Manual 8.1.3 Calculation If the Factor Method protocol, a modified Beer-Lambert equation is used to calculate concentrations with user- defined measuring absorbance and factor as follows, with or without baseline correction (optional): Without baseline correction: c = A x ԑ / b With baseline correction: c = (A - A ) x ԑ... - Page 32 Operating Manual On the Sample Data tab page of Std. Curve method protocol (Figure 27), the data information parts have the features below (Table 18). The function icons will be activated after finishing the protocol setting on the Custom Setting tab page. Information tabs Insert sample name Sample data information...
- Page 33 Operating Manual Table 19. Standard tab page information. Features Description This is the concentration column. The unit is based on the self-defined value on the [conc.] Custom Setting page. The value is inserted by the user. The absorbance measured at different wavelength according to the protein method. Avg.
- Page 34 Operating Manual 8.2.2 Protocol Operation On the main menu, tap on the More Assays icon then the Standard Curve Method icon to enter protocol selection. Ensure the sample window and the cover window on the detection arm are clean. Insert Analysis Wavelength (required). Insert the Units (required).
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Page 35: Uv-Vis Method
Operating Manual 8.2.3 Calculation In the protocol of Standard Curve Method, the concentration is calculated by the absorbance values measuring in the user-defined wavelength and the established standard curve. The standard curve can be in types of linear, interpolation, or 2 order polynomial. - Page 36 Operating Manual Table 20. Sample data tab page information. Features Description These columns will automatically turn to the user-defined wavelength after finishing A--- the protocol setting on the Custom Setting tab page. After measuring samples, it will show the absorbance of the samples in user-defined wavelength. Name The sample name can be inserted here.
- Page 37 Ensure the sample window and cover window on the detection arm are clean. Insert required Analysis Wavelength. Spex NanoSNAP affords a maximum of 8 wavelengths. Add appropriate solution of at least 1 µL and select the Blank icon to establish blank data.
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Page 38: System Setting
System Settings Tap on the System icon on the Main Screen to enter the System setup section as shown in Figure 35 below. Here you can adjust several parameters for the Spex NanoSNAP. Figure 35. System Overview Table 21. System menu setup icons. -
Page 39: History Information
Operating Manual 10. History Information Tap on the History icon on the main screen to enter the History list information screen. A sample screen is shown in Figure 36 below. Lock symbol Figure 36. History List Overview All stored reports are shown in the history list. If all the reports can't be shown on one page, you can slide up or down on the screen to check the rest of the reports. -
Page 40: User Folder Management
Operating Manual 11. User Folder Management Tap on the User icon on the main screen to enter the user folder management screen. A sample screen is shown in Figure 37 below. Lock symbol Figure 37. User Folder Overview All protocols are stored inside the user folders. There are 8 user folders displayed on the page. If there are more than 8 user folders registered in the system, you can slide up or down on the screen to see other pages. -
Page 41: Using A Usb Flash Drive As A User Folder
It is recommended to format your flash drive in the FAT or FAT32 file system prior to using it with the Spex NanoSNAP.) You can also use the USB flash drive to transfer the reports between the Spex NanoSNAP and your computer. -
Page 42: Troubleshooting
Operating Manual 13. Troubleshooting Problem Cause Action Power is not reaching the system Check power source voltage The display is off even Power cord is not plugged into the socket when the power is Reconnect the power cord properly switched on Faulty power adapter Return the unit for service The solutions are not homogeneous and well-... -
Page 43: Error Messages
Operating Manual 13.1 Error Messages Message Cause Action Er01 Did not receive SD card signal in Return the unit for service ERR_NO_SDCARD 1 second continuously Automatically detected numerical Er02 anomalies. Insufficient light source Return the unit for service ERR_SELFTEST_NG intensity or excessive noise. Er03 Optics module problem Reboot the unit... -
Page 44: Appendix A: Technical Specifications
Operating Manual Appendix A: Technical Specifications Optics Information Sample Volume 1 µL minimum volume Sample Number Pathlength 0.5 mm or 0.05 mm, selectable Light Source Pulsed Xenon flash lamp Detector Type 2048 element CMOS Wavelength Range 190 to 1,000 nm Bandwidth 1.3 nm Wavelength Accuracy... -
Page 45: Appendix B: Ordering Information
Operating Manual Appendix B: Ordering Information Equipment Product Name Part Number Spex NanoSNAP UV-Visible Microvolume Spectrophotometer 83057-02 Maintenance kit (hydrophobic reagent) 83057-03 DNACON solution 260/280 reference 99959-77 spexsampleprep.com 65 Liberty Street Metuchen, NJ 08840 Phone: +1.732.549.7144 Connect with us spexsales@antylia.com...
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