Fisher Bioblock Scientific ALPHA 1-4 LD-2 Operating Manual
  1. Manuals
  2. Brands
  3. Fisher Bioblock Scientific Manuals
  4. Dryer
  5. ALPHA 1-4 LD-2
  6. Operating manual

Fisher Bioblock Scientific ALPHA 1-4 LD-2 Operating Manual

Freeze dryer
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the ALPHA 1-4 LD-2 and is the answer not in the manual?

Questions and answers

Summary of Contents for Fisher Bioblock Scientific ALPHA 1-4 LD-2

  • Page 2 Gefriertrocknungsanlagen OPERATING MANUAL Freeze Dryer ALPHA 1-4 LD-2 (Best.-Nr. 101041 / 101141 / 101241) ALPHA 2-4 LD-2 (Best.-Nr. 101042 / 101142 / 101242) M A R T I N C H R I S T Gefriertrocknungsanlagen GmbH...
  • Page 3 In case of inquiries or repair please state the above numbers. For service please contact: MARTIN CHRIST Gefriertrocknungsanlagen GmbH An der unteren Sö se 50, D-37520 Osterode Tel. (05522) 5007-25, Telefax (05522) 5007-12 Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 2...
  • Page 4 The freeze dryer is equipped with user-friendly options which make the operation easier for you. The newly designed ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2 is a universally usable CHRIST freeze dryer for laboratories, R&D departments and scientific institutes. The well proven range of accessories allows application oriented configuration of the equipment especially for routine use.

  • Page 5: Table Of Contents

    2.7.1 Connection of vacuum pump and installation of accessory components 2.7.2 Functional components and control elements 2.7.3 Preparation for evacuation 2.7.4 Evacuation Error messages Error resolution Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 4...
  • Page 6 Freezing separately and drying in ampoules using a manifold Maintenance Vacuum pump Exhaust filter Ice condenser chamber Rubber valves Condenser for cooling liquid Adjustment of the vacuum measuring sensor TPR 265 Cleaning Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 5...
  • Page 7 … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … Chemical properties of PLEXIGLAS (acrylic glass, material of drying chamber) Disinfectant spray INCIDUR Return Declaration Declaration of Contamination Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 6...

  • Page 8: General Information

    − Freeze drying − Final drying The freeze dryer ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2 is suitable for drying of e. g. bacteria and virus cultures, blood plasma, serum fractions, antibodies, sera, vaccines and pharmaceutical products such as chloramphenicol, streptomycin, vitamins, ferments as well as plant extracts for biochemical tests.

  • Page 9: Technical Specifications Of Freeze Dryer Alpha 1-4 Ld-2 / Alpha 2-4

    Refrigerant: see label on the back of the unit Connection requirements: 230 V, 50 – 60 Hz Electric connection: (others on request) and central grounding connection Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 8...
  • Page 10 − Commissioning of the unit will be carried out on request and charged at cost. − Installation of the exhaust pipe of the vacuum pump (not necessary when using an exhaust filter). Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 9...

  • Page 11: Standards And Regulations

    The limb can only be detached from the surface by applying heat. Liquid should not be used. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 10...

  • Page 12: Prohibited Freeze Drying Processes

    Breaking glass or bursting vessels can cause dangerous situations during freeze drying. 7. Operation in locations with danger of explosion. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 11...
  • Page 13 Infectious, toxic, pathogenic and radioactive substances must only be dried in suitable vessels. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 12...

  • Page 14: Installation And Commissioning Of The Unit

    For checking the earth connection there is a screw for equalising the ground potential on the back panel of the freeze dryer. The check can be carried out by means of an appropriate measuring device. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 13...

  • Page 15: Defrosting Water

    The collected oil is visible in the inspection glass and is drained via the discharging screw. (Please refer to separate operating manual of the vacuum pump!) Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 14...

  • Page 16: Initial Start-Up

    5 Vacuum hose 6 Vacuum pump The accessory components are connected up to the freeze dryer ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2 according to the process flow diagram above. Centering rings and clamping flanges with wing nuts are used as connecting elements (small flange...
  • Page 17 Tighten the screw of the (the centering ring must clamping flange by hand. be positioned correctly!) and place clamping flange over the connection and press together. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 16...
  • Page 18 5 unheated shelves as well as individually sealable rubber valves for round bottom flasks. Example: Accessory for drying on 2 unheated shelves with sealing device for injection vials. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 17...
  • Page 19 Stoppering device with 1 shelf, 250, unheated, max. 4 Drying chamber shelves can be used Drying vessel, e.g. injection vial Drip tray Base plate ALPHA 1-4 LD-2 ALPHA 2-4 LD-2 Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 18...
  • Page 20 24 rubber valves Drying vessel, e.g.. wide neck filter Drying chamber, e.g. round bottom flaks Drying vessel, e.g. injection vial ALPHA 1-4 LD-2 ALPHA 2-4 LD-2 Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 19...
  • Page 21 Drying vessel, e.g. Ampoule round bottom flasks Drying vessel, e.g. injection vial 5 shelves 360, unheated Product dish Connection flange ALPHA 1-4 LD-2 ALPHA 2-4 LD-2 Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 20...

  • Page 22: 2.7.2 Functional Components And Control Elements

    Interface connection (Option, seperate accessory) Name-plate Connection for Potential equalizing screw vacuum measuring and central grounding sensor TPR 265 connection Connection for mains cable and fuse Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 21...

  • Page 23: Preparation For Evacuation

    Meaning, possible cause ER10 Compressor 1 overpressure Compressor turns off (until overpressure is reducted) ER 11 Compressor 2 overpressure Compressor turns off (until overpressure is reducted) Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 22...

  • Page 24: Error Resolution

    Cleaning; call service if lid seal is damaged (Contact on page. 2) 3. Condensate drain valve defect (does Cleaning; exchange O-rings not close) condensate drain valve Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 23...

  • Page 25: Control System

    LC display showing the most important process data (temperature of ice condenser, vacuum) Conversion of temperature and vacuum in accordance with the vapour pressure curve above ice RS-232 communications interface (PC) is available as an option. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 24...

  • Page 26: Mains Switch

    Button "°C" "° C mbar" Button On the freeze dryer ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2 the vacuum in the drying chamber or alternatively the ice condenser temperature can be displayed. The change- over is carried out by pressing the "° C " or “ mbar” button.

  • Page 27: Connection Of The Vacuum Measuring Sensor Tpr 265

    (atmosphere) is displayed after switching on the unit (see point 6.6 “ Adjustment of vacuum sensor TPR 265” ). Vacuum measuring sensor TPR 265 Connecting plug for vacuum measuring sensor TPR 265 Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 26...

  • Page 28: General Information On Freeze Drying

    (simultaneously). Below this point, i.e. the vacuum is lower than 6.11 mbar, the ice is converted directly from a solid to a gaseous phase on reaching the sublimation pressure curve. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 27...
  • Page 29 0,021 0,0012 1,510 0,200 0,018 0,0010 1,370 0,180 0,016 1,250 0,160 0,014 1,140 0,140 0,012 Conversion table " Vapour pressure above ice " (sublimation pressure curve) Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 28...

  • Page 30: Freezing

    Freezing The freezing of small quantities of product is carried out inside the ice condenser chamber of the ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2. Larger quantities are pre-frozen in a deep-freeze. If liquids are to be dried in bottles with a layer thickness of more than 1 cm we recommend that pre-freezing is carried out with a shell or spin-freezing-device in a cooling bath (see previous picture).

  • Page 31: Main Drying

    However, small quantities of water vapour are also pumped off by the vacuum pump. Therefore, the vacuum pump is equipped with a gas ballast device. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 30...
  • Page 32 During the next quarter of the main drying phase 50 % of the remaining water content is condensed. This continues until the drying curve approaches the time axis asymptotically. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 31...
  • Page 33 The constraints caused by the former can be seen in the following diagram. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 32...
  • Page 34 In so far as there are heated shelves, otherwise via radiation on the walls of the vessel or the exposed surface of the product Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 33...

  • Page 35: Final Drying

    The defrosting water is drained through the defrosting water valve at the left side of the unit. To do this, a hose is put onto the nozzle. The defrosting water is collected in a vessel. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 34...

  • Page 36: Description Of The Freeze Drying Processes

    The ice condenser chamber should be wiped If necessary. For pre-cooling the ice condenser the ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2 is switched The rack is put onto the bottom plate. For smaller samples it is advisable to pre-cool the shelves in order to avoid a partial thawing during the evacuation process.

  • Page 37: Freezing Separately And Drying In Round Bottom Flasks

    The drying chamber with 8 connections is mounted on the sealing ring on the bottom plate of the ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2. Manifolds with standard ground joints NS 45/40 are connected via the ground-in socket of the acrylic chamber.
  • Page 38 The remaining glass from the ampoule is removed and the hose is closed with a dummy plug. In this way one ampoule after the other can be sealed or new ampoules can be connected. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 37...

  • Page 39: Maintenance

    Special attention must be paid to the rubber valves. If the valves are stiff, they must be dismantled, cleaned, slightly greased with vacuum grease and reassembled. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 38...

  • Page 40: Condenser For Cooling Liquid

    TPR 265 must be in exactly the same place as it will be used in later! − Press the conversion button "=" of ALPHA 1-4 LD-2 / ALPHA 2-4 LD-2 and set the transition point in the display from "A-FF" to "A-or" (i. e. between "FF" and "Overrange") using the potentiometer "ATM"...

  • Page 41: Cleaning

    − Mount the TPR 265 on the vacuum pump and evacuate for approx. 10 minutes. − Set the transition point in the vacuum display of ALPHA 1-4 LD-2 / ALPHA 2-4 LD- 2 between 0.0026 mbar and 0.0030 mbar using the potentiometer "HV" of the TPR 265.

  • Page 42: 6.7.3 Maintenance Of The Condensate Drain Valve

    3. Oil level of vacuum pump 4. Accessories, especially changes like corrosion, cracks, wear and tear of material etc. Furthermore, an earth conductor check must be carried out regularly. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 41...

  • Page 43: Power Failure

    Check the oil level in the vacuum pump, change oil if dirty, carry out pressure test. Check if the whole surface of the ground-in socket of the acrylic chamber has been greased evenly with vacuum grease. Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 42...

  • Page 44: Unit Does Not Function

    − Is the mains switch plugged in? − Check the fuses F1 and F2 (draw in the power supply plug on the back of the unit). Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 43...

  • Page 45: Insufficient Ice Condenser Temperature

    Sufficient air circulation is necessary. M A R T I N C H R I S T Gefriertrocknungsanlagen GmbH Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203, Page 44...
  • Page 46 Connection condensate drain Time relais for 2. stage of compressor Mains input 230 V 50 Hz External ground connection Mains switch Control System LD-2 Main fuse 230 V 10 A Operating Manual Freeze Dryer ALPHA 1-4 LD-2 / 2-4 LD-2, Version 0203...
  • Page 47 Declaration of Contamination Of Freeze Dryers, Vacuum-Concentrators, Centrifuges, Accessories and Vacuum Pumps. This declaration may only be filled in and signed by authorised staff. Repair Order dtd. : Order No. : Type of unit : Serial No. : Type of unit : Serial No.
  • Page 48 Return Declaration Following declarations are for the health and safety of our employees. Fill in the forms and attach them when returning freeze dryers, centrifuges, spare parts and accessories. Please understand that we cannot carry out any work before we have the declarations.

This manual is also suitable for:

Alpha 2-4 ld-2101041101141101241101042101142 ... Show all

Table of Contents