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Instruments for Electrophoresis
INSTRUCTION MANUAL
BlueVertical™ PRiME™
Vertical Electrophoresis Chamber
SERVA Electrophoresis GmbH
Carl-Benz-Str. 7
D-69115 Heidelberg
Phone +49-6221-138400, Fax +49-6221-1384010
e-mail: info@serva.de
http://www.serva.de

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  • Page 1 Line Instruments for Electrophoresis INSTRUCTION MANUAL BlueVertical™ PRiME™ Vertical Electrophoresis Chamber SERVA Electrophoresis GmbH Carl-Benz-Str. 7 D-69115 Heidelberg Phone +49-6221-138400, Fax +49-6221-1384010 e-mail: info@serva.de http://www.serva.de...
  • Page 2 First check that the apparatus has been received complete and undamaged following shipment. Any faults or losses must be notified to responsible SERVA Electrophoresis GmbH distributor immediately. SERVA Electrophoresis GmbH Heidelberg cannot accept responsibility for goods returned without prior notification.
  • Page 3: Table Of Contents

    Contents Packing List Specifications Operational conditions Using the vertical gel electrophoresis unit 4.1. Safety precautions 4.2. General care and maintenance 4.3. Gel and buffer volumes/running conditions 4.4. Performing electrophoresis 4.4.1. Insertion of pre-cast gel cassettes into the inner core running unit 4.4.2.
  • Page 4: Packing List

    ACKING BlueVertical™ PRiME™ Cat. no.: BV 104 No. of Description Cat. no. Items Main Unit BV 104 Dummy Plate BV 104-7 2. Specifications • Rugged acrylic construction • All acrylic joints chemically bonded • Doubly insulated cables, rated safe up to 1000 volt •...
  • Page 5: Operational Conditions

    Table 1: Specifications Volume inner buffer chamber 200 ml Volume outer buffer chamber 450 ml Voltage (max) 500 V Current (max) 250 mA Operating temperature 4 °C – 65 °C Electrodes Platinum wire (0.2 mm, 99.99 % pure) Dimensions 16 x 15.6 x 9.5 cm (WxHxD) Weight 1.2 kg 3.
  • Page 6: General Care And Maintenance

    Any leakage will cause dampening of the tissue paper. If any leakage is seen do not attempt to repair or use the apparatus, but notify SERVA Electrophoresis GmbH Heidelberg resp. the SERVA Electrophoresis GmbH distributor immediately.
  • Page 7: Performing Electrophoresis

    4.4. Performing electrophoresis 4.4.1. Insertion of precast gel cassettes into the inner core running unit • Clean the silicone gasket, located in the inner core unit. If the gasket becomes unseated from its groove simply press it back into place. •...
  • Page 8: Sample Loading

    • Connect the electrophoresis apparatus to the power pack and connect the power pack to the mains supply, e. g. SERVA BluePower 500x4 (BP-500x4). Turn all settings to zero before turning on the mains supply. Adjust the controls to the desired settings.
  • Page 9 Ensure that the connectors are clean and dry before usage or storage (see chapter 4.2). For further information about the SERVA BlueLine, please contact the technical service of SERVA Electrophoresis GmbH in Heidelberg, phone Tel.: +49 (0)6221...
  • Page 10: Recommended Reagents For Vertical Electrophoresis

    5. Recommended reagents for vertical electrophoresis SERVA reagents for electrophoresis underly stringent quality and application control to ensure best performance and results. We recommend the usage of SERVA electro- phoresis reagents especially along with BlueLine electrophoresis instruments as the quality of consumables is fine-tuned to the equipment (application tests).
  • Page 11 Dithioerythritol (DTE) 20697 SERVA Blue G 35050 SERVA Blue R 35051 Bromophenol Blue-Na-salt 15375 SDS Solution, 20 % 20767 A comprehensive range of SERVA products for electrophoresis is available listed in the Main Catalogue available from SERVA Electrophoresis. Please inquire.

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