1. Manuals
  2. Brands
  3. ACQUITY Manuals
  4. Security Sensors
  5. WKB230245
  6. How to clean

ACQUITY WKB230245 How To Clean

Hide thumbs
1
2
3

Advertisement

Table of Contents

How to clean an ACQUITY UPLC ELS Detector - WKB230245

OBJECTIVE or GOAL

Clean the ELS detector—as part of routine maintenance or in response to degraded performance.

ENVIRONMENT

• ACQUITY UPLC ELS Detector

PROCEDURE

Cleaning the drift tube

1. In the console, select ELS Detector from the system tree.
2. In the temperatures area, click the Nebulizer field, which contains an underlined set point value. The ELSD Nebulizer
Setup dialog box appears.
3. Select On from the nebulizer mode drop-down list.
4. Specify a nebulizer power percent level of 75%.
5. In the temperatures area, click the Drift Tube field, which contains an underlined set point value. The ELSD Drift Tube
Temperature dialog box appears.
6. Specify a drift tube heater temperature set point of 100 °C (212 °F), and then click OK
.
7. Remove the column.
8. Flush the system with 100% HPLC-grade water or a mixture of organic solvent compatible with your mobile phase for
60 minutes at 1 mL/min.
9. Reset the system to operating conditions.
10. Reinstall the column.
11. Evaluate chromatography.

Cleaning the nebulizer using sonication

1. Stop the liquid flow, and remove the solvent inlet line.
2. Power-off the detector and disconnect the power cable from the rear panel.
3. Open the detector door, gently pulling its right edge toward you.
4. Use a 5/16-inch wrench to loosen the compression screw that holds the inlet tubing in place, and then disconnect the
solvent inlet tubing.
https://support.waters.com/KB_Inst/Chromatography/WKB230245_How_to_clean_an_ACQUITY_UPLC_ELS_Detector
Updated: Wed, 31 Aug 2022 14:32:45 GMT
Powered by
1

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the WKB230245 and is the answer not in the manual?

Questions and answers

Summary of Contents for ACQUITY WKB230245

  • Page 1 How to clean an ACQUITY UPLC ELS Detector - WKB230245 OBJECTIVE or GOAL Clean the ELS detector—as part of routine maintenance or in response to degraded performance. ENVIRONMENT • ACQUITY UPLC ELS Detector PROCEDURE Cleaning the drift tube 1. In the console, select ELS Detector from the system tree.
  • Page 2 5. Stop the gas flow. 6. Push in the quick-disconnect tubing fitting on the right-hand side of the nebulizer, and pull out the gas inlet tubing. 7. Push in and turn the nebulizer counterclockwise so that the quick-disconnect tubing fitting is at the twelve o’clock position, and then remove it from the nebulization chamber.
  • Page 3 11. Place the beaker in an ultrasonic bath for 10 to 15 minutes. 12. Remove the beaker from the bath. 13. Remove the nebulizer from the beaker. 14. Insert the gas inlet tubing into the quick-disconnect tubing fitting on the right-hand side of the nebulizer, and place the nebulizer in a dry beaker so that it stands up.

Table of Contents