Amersham Pharmacia Biotech AKTA design pH/C-900 User Manual
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Amersham Pharmacia Biotech AKTA design pH/C-900 User Manual

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Monitor pH/C-900
User Manual
18-1120-06

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Summary of Contents for Amersham Pharmacia Biotech AKTA design pH/C-900

  • Page 1 Monitor pH/C-900 User Manual 18-1120-06...
  • Page 2 Be sure not to proceed until the Amersham Pharmacia Biotech group which supplies instructions are clearly understood and all stated them. A copy of these terms and conditions of sale is conditions are met.

  • Page 3: Table Of Contents

    Contents Contents Introduction General ........................1 Safety ........................1 Installation Unpacking......................2 General precautions ....................2 Installing the conductivity cell ................2 Installing the pH electrode ..................3 Connecting electrical signal cables .................4 Connecting to communication link ................4 Connecting to supply voltage.................5 Preparing the instrument for use................5 Operation On/off ........................6 Menu selection and settings ...................6...
  • Page 4 Contents Reference information Description ......................19 Instrument....................19 pH electrode....................20 Conductivity cell ..................20 Menus........................21 Check menu ....................21 B.1.1 Check Monitor Cell and Electrode ...........21 B.1.2 Check Service Mode ..............21 Setup menu ....................21 B.2.1 Setting up conductivity temperature compensation....21 B.2.2 Calibrating the conductivity cell ..........22 B.2.3 Entering the conductivity cell constant ........23 B.2.4...

  • Page 5: Introduction

    Introduction 1 Introduction 1.1 General Monitor pH/C-900 is a high precision on-line monitor for measurement of pH and conductivity in liquid chromatography. The pH/C-900 features: • Fast response • High accuracy and reproducibility • Flow cells with low dead volume •...

  • Page 6: Installation

    Installation 2 Installation 2.1 Unpacking Unpack the instrument and check the items against the supplied packing list. Inspect the items for obvious damage which may have occurred during transportation. It is recommended that all packing materials should be retained if onward transport of the instrument is expected.

  • Page 7: Installing The Ph Electrode

    Installation 2.4 Installing the pH electrode (optional in ÄKTApurifier systems) Mounting the flow cell holder 1 Hook the flow cell holder on the right hand side of the housing. Secure it with the slide clamp. If the flow cell holder is not used, the flow cell must still be installed at an angle of 30°...

  • Page 8: Connecting Electrical Signal Cables

    Installation 2.5 Connecting electrical signal cables The sockets for electrical signals are located on the rear panel. Probe Ground Analogue out 0-1V Mains On/off Conductivity pH Cond UniNet 1 Flow Cell Connecting to chart recorder (if used) The external chart recorder outputs for pH and conductivity from the monitor are 0 –...

  • Page 9: Connecting To Supply Voltage

    Installation 2.7 Connecting to supply voltage 1 Make sure the on/off switch is in the OFF-position ( 2 Connect the supplied mains cable between the instrument and a grounded mains socket. Any voltage 100–240 V AC, 50–60 Hz can be used. WARNING! The instrument must be connected to a grounded mains socket.

  • Page 10: Operation

    Operation 3 Operation 3.1 On/off Switch on the instrument at the mains switch on the rear panel. Selftest Please wait... At switch on, the instrument performs a selftest and then starts calibration. After approx. 30 seconds the display shows Monitor pH/C900 V1.00 and the instrument is ready to use.

  • Page 11: Main Menu Overview

    Operation Return to main menu Pressing repeatedly, always returns to the which is main menu 1 the main operating menu. Main menu 1 Main menu 2 Select value A cursor below a text or numerical value shows what is affected by the dial.

  • Page 12: Reading Ph And Conductivity Values

    Operation 3.4 Reading pH and conductivity values The main operating menu shows the conductivity as a percentage of Cond Temp full scale together with the current temperature in the flow cell, and 25.4% 22.9°C 11.50 the pH value. If the pH value is not stable or is changing, an asterisk is displayed after the value, e.g.

  • Page 13: Calibrating Ph

    Operation 3.6 Calibrating pH (optional in ÄKTApurifier systems) A good laboratory routine is to calibrate the instrument once a day, when the electrode is replaced and if the ambient temperature is changed. The pH monitor is calibrated using standard buffer solutions in a two point calibration.
  • Page 14 Operation 8 The sub menu Calibrated Calibrated Electrode Voltage (mV) The calibration curve Slope 97.5% 8 mV shows the Electrode Slope shows the relationship between pH and the slope of the calibration curve output signal from the +500 mV where 100% corresponds to monitor in mV.

  • Page 15: Calibrating Conductivity

    Operation 3.7 Calibrating conductivity The cell constant for the particular flow cell is written on the flow cell packaging. Refer to section B.2.3 in Reference information for how to enter the cell constant. Adjustment of the cell constant is only necessary when the monitor is to be used to determine specific conductivity with high accuracy.

  • Page 16: Storage And Shut-Down

    Operation 3.9 Storage and shut-down CAUTION! Never leave the pH electrode in the flow cell for any period of time when the system is not used, since this may cause the glass membrane of the electrode to dry out. Dismount the pH electrode from the flow cell and fit the end cover filled with a 1:1 mixture of pH 4 buffer and 1 M KNO Do NOT store in water only.

  • Page 17: Maintenance

    Maintenance 4 Maintenance WARNING! Disconnect the power supply before attempting to replace any item on the instrument during maintenance. CAUTION! Only spare parts approved or supplied by Amersham Pharmacia Biotech may be used for maintaining and servicing the instrument. 4.1 Periodic maintenance Interval Action (see procedures below) Every 6 month...

  • Page 18: Changing Ph Electrode

    Maintenance If these procedures fail to rejuvenate the electrode, the problem is most likely a clogged liquid junction. 1 Heat a 1 M KNO solution to 60 - 80°C. 2 Place the electrode tip in the heated KNO solution. 3 Allow the electrode to cool while immersed in the KNO solution before re-testing.

  • Page 19: Trouble Shooting

    Trouble shooting 5 Trouble shooting 5.1 General When contacting Amersham Pharmacia Biotech for support, state Monitor pH/C900 the program version of the instrument, which is shown for 2 seconds V1.00 during switch-on. WARNING! The instrument must not be opened by the user. It contains high voltage circuits which can be capable of delivering a lethal electric shock.
  • Page 20 Trouble shooting Fault Action 7 Check the slope (see section 3.6 Calibrating pH ). If it is outside the range 80–105% or the asymmetry potential deviates more than 60 mV from 0 mV, clean the pH electrode. Recalibrate and if the problem persists, replace the pH electrode.

  • Page 21: Error Messages

    Trouble shooting Fault Action Conductivity measurement 1 Clean the flow cell according to procedure in section with the same buffer appears 4.5 Cleaning the conductivity flow cell . to decrease over time 2 The ambient temperature may have decreased. Use a temperature compensation factor, see section B 2.1 Setting up conductivity temperature compensation , in Reference information .
  • Page 22 Trouble shooting Message Action 1 Check that the pH electrode is connected. Bad pH cell or not 2 Replace the pH electrode. connected 1 Check that the conductivity cell is connected. Recalibrate. Temp cell is bad 2 The calibration value differs from the predefined calibration or not connected value by more than ±5 °C.

  • Page 23: Reference Information

    Reference information Reference information Description A.1 Instrument Monitor pH/C-900 is an on-line monitor for measurement of pH (optional in ÄKTApurifier)and conductivity. The monitor can work with standard glass pH electrodes with a built in liquid-filled reference electrode and BNC connector. Probe Ground Analogue out 0-1V...

  • Page 24: Ph Electrode

    Reference information A.2 pH electrode The pH electrode is of the sealed combination double junction type. Sealing It contains a sealed Ag/AgCl Internal electrolyte reference which cannot be refilled, bridge containing 4M KCl saturated an internal electrolyte bridge of 4M with AgCl KCl saturated with Ag/AgCl, an Silicone sealing...

  • Page 25: B Menus

    Reference information Menus B.1 Check menu B.1.1 Check Monitor Cell and Electrode A check of the conductivity cell and pH electrode can be performed. Check Monitor Cell and Electrode 1 Select main menu , press OK. Check 2 Select sub menu , press OK.

  • Page 26: Calibrating The Conductivity Cell

    Reference information The factor is expressed in percentage increase of conductivity per °C increase in temperature. If the temperature compensation factor is unknown, a general approximate value of 2% can be set for many common salt buffers. Set the value to 0 for no temperature compensation. 5 Select sub menu , press OK.

  • Page 27: Entering The Conductivity Cell Constant

    Reference information 5 Select main menu Conductivity of 1.00 M NaCl at 20–30°C , press OK. Setup 6 Select sub menu Setup Conductivity Setup Conductivity press OK. 7 Select sub menu Setup Adjust Cond Setup Adjust Cond (83.53mS/cm) press OK. 8 A warning message Warning! This will will be displayed,...

  • Page 28: Setting Up Ph Temperature Compensation

    Reference information B.2.4 Setting up pH temperature compensation The relationship between pH and the output signal from the pH electrode is temperature dependent. For more accurate measurements during temperature changes, the pH measurement can be temperature compensated. In normal applications, if the temperature of the buffers and calibration buffers are identical, temperature compensation does not need to be on.

  • Page 29: Setting Up The Temperature Display

    Reference information B.2.7 Setting up the temperature display Display of the temperature in the conductivity flow cell, in the main operating menu, can be enabled or disabled. 1 Select main menu , press OK. Setup 2 Select sub menu , press OK. Setup Temperature Setup Temperature 3 Select sub menu...

  • Page 30: Setting And Using The Alarm Timer

    Reference information B.3 Setting and using the alarm timer You can set the alarm function to either a fixed alarm time or using a count-down timer. The default or previous value is shown in parentheses. Alarm/Timer 12:30:52 1 Select main menu , press OK.

  • Page 31: Menu Overview

    Reference information Menu overview Cond Temp 25.4% 22.9°C 11.5 4.000 mS/cm 22.9°C ––– 25.4% Set Cond Scale 100% (50.00mS/cm) Set cond scale 0% (1.00mS/cm) Calibrate pH Calibr pH Buffer 1 (7.00–10.00) Calibr pH Buffer 2 Calibrated Electrode Slope 97.5% 8.0mV Telephone Service 012345678901 Set pH Full Scale...

  • Page 32: C Technical Specifications

    Reference information Technical specifications The full specifications apply only after at least 1 hour warm-up. Operating data pH measurement pH range 0 to 14 (spec. valid between 2 and 12) Accuracy temperature compensated ±0.1 pH within +4 to +40°C not temperature compensated ±0.2 pH within +15 to +25°C ±0.5 pH within +4 to +15°C and +25 to...
  • Page 33 Reference information Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100% Ethyl acetate, 100% Hexane, and 100 % Tetrahydrofuran (THF). Conductivity cell Max Flow rate 100 ml/min Max Pressure 5 MPa (50 bar, 725 psi) Back pressure Max.
  • Page 34 Biotech except for alterations described in the user manual • used as “stand alone” unit or connected to other CE labelled Amersham Pharmacia Biotech instruments or other products as recommended. S afety standards This product meets the requirement of the...

  • Page 35: D Accessories And Spare Parts

    Reference information Accessories and spare parts Item Quantity per pack Code no. Monitor pH/C-900 without pH electrode and flow cells 18-1107-76 pH electrode, round tip 18-1111-26 pH electrode with flow cell and 18-1134-84 holder, round tip pH flow cell, incl. dummy electrode 18-1112-92 Dummy electrode, round tip 18-1111-03...
  • Page 40 Short instructions The following short instructions are intended as a guide to users who are fully familiar with safety precautions and operating instructions described in this manual. The instructions assumes that the instrument is installed according to the installation instructions. Select by means of the mains Switch on the instrument...

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