AKTA design P-900 User Manual
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AKTA design P-900 User Manual

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Pump P-900
User Manual
18-1120-04

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Summary of Contents for AKTA design P-900

  • Page 1 Pump P-900 User Manual 18-1120-04...
  • Page 2 Important user information Trademarks ÄKTA and UNICORN are trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries. Meaning: Consult the instruction manual to avoid Amersham is a trademark of Nycomed Amersham plc. personal injury or damage to the product or other Pharmacia and Drop Design are trademarks of equipment.

  • Page 3: Table Of Contents

    3.10 Restart after power failure ...................16 Maintenance Periodic maintenance ...................17 Cleaning-in-place ....................17 General care......................17 Replacing the piston seal ..................18 Running-in a new piston seal................22 Replacing a damaged piston ................22 Cleaning the inlet and outlet check valves ............23 Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 4 About this manual This manual comprises two parts; a practical part (sections 1 – 5) and a reference part (sections A – D). Sections 1 – 5 contain the necessary information for operating the instrument. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 5: Introduction

    Introduction 1 Introduction 1.1 General Pump P-900 is the collective name for a pump family. The family members are: • P-901 stand-alone or system mounted Used as stand-alone, or in ÄKTA ™ explorer 100. Equipped with four pump heads in two pump modules.
  • Page 6 Introduction All P-900 models are high performance laboratory pumps for use in liquid chromatography and other applications where accurately controlled liquid flow is required. Double mode can be selected to double the flow rate range. Pump P-900 features: • P-901 - Pressure range 0 –...

  • Page 7: Safety

    WARNING! The instrument must not be opened by the user. It contains high voltage circuits which can give a lethal electric shock. WARNING! The instrument must be connected to a grounded mains socket. WARNING! NaOH is injurious to health. Avoid spillage. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 8: Installation

    2.3 Connecting electrical signal cables The sockets for electrical signals are located on the rear panel. Valve 1 Valve A Valve B Pressure, analog UniNet 2 Remote UniNet 1 Mains out 0 - 1V Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 9: Connecting To Communication Link

    PC and the termination plug. The UniNet 1 link connects, in series, the PC with Pump P-900 and other instruments. The termination plug is connected to the last instrument in the chain.

  • Page 10: Installing The Piston Seal Rinsing System

    A. Slowly suck up rinsing solution to the syringe. When rinsing solution starts to enter the syringe, continue to draw a few millilitres. 5 Loosen the syringe and immerse the tubing in the rinsing solution. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 11: Connecting The Inlet Tubing

    Check that there is no air left in the inlet tubing. 6 Repeat steps 3 to 5 for pump module B, if fitted. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 12: Running-In The New Pump

    Flow rate Time Set Flow Rate (0.00ml/min) 0.10 P-901 40 ml/min 10 minutes P-903 4 ml/min 10 minutes 0.10ml/min 0.00MPa 0.0%B 5 Stop the flow by setting the pump in mode. 0.00ml/min 0.00MPa 0.0%B Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 13: Connecting The Pump Outlet Tubing

    2.11 Installation of accessories Mixer M-925 and up to three 2-way Switching Valves SV-903 can be connected to the pump: 0 0 , , 6 6 Switch Valve SV-903 Mixer M-925 Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 14 SV-903 for one-pump gradients is connected to Valve A. Valve 1 Valve A Valve B Pressure, analog UniNet 2 Remote UniNet 1 Mains out 0 - 1V Out to system 0 0 , , 6 6 Fluid inlet tubing Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 15: Operation

    Please wait... synchronization. Pump P-900 Open the purge valves (see section 2.8) to avoid high pressure during V1.00 synchronisation which is done at the speed of 0.1 ml/min, and press OK.

  • Page 16: Main Menu Overview

    Setup Setup language, pressure limits, etc. See Reference information section B.2. Set different timer options. The pump can be started or stopped at Alarm/Timer 12:30:52 Bzz12:33:00 set times. See Reference information section B.3. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 17: Starting And Stopping The Pump

    If the eluent is to be changed, see section 3.9 Changing eluent Note: The pump may not work if the buffer vessels are sealed. Do not close the vessels off completely. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 18: Setting The Flow Rate And Starting The Pump

    2 The display shows , press OK. Set Concentration B Set Concentration B (0.0%B) 3 Set the %B value, press OK. In UNICORN, select instruction Gradient . Set length to zero. System Control:Manual:Pump Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 19: Running A Simple Gradient

    Overnight storage: The pump can be left filled with a buffer. Weekend and Long time storage: Flush the pump with water and then fill it with 20% ethanol. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 20: Changing Eluent

    If the power supply to the instrument is interrupted, the instrument automatically restarts itself doing selftest and prompting for synchronisation. All values under the menu are retained. Other Setup values, e.g. flow rate are reset. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 21: Maintenance

    1 minute. If there is a lot of air in the inlet tubing, stop the pump and perform the procedure in section 2.8 Purging the pump. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 22: Replacing The Piston Seal

    CAUTION! Read the following instructions carefully. The individual parts of the pump head can be assembled incorrectly. Take care to ensure that the orientation of each part is correct before continuing with the next instruction. Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 23 9 Remove the two screws securing the drain plate and the rinse chamber. Remove and discard the rinsing membrane. Remove the rinse chamber. For P-903, remove also the support washer. Rinsing membrane Drain plate Rinse chamber Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 24 Rinse membrane Rinse chamber Rinse chamber Rinse chamber Rinse chamber outlet outlet Inlet Support washer check valve Inlet Piston seal check valve Jointing ring Piston seal Outlet check valve Outlet check valve P-901 P-903 Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 25 19 Refit the tubing of the piston seal rinse system. 20 The pump should now be purged and the new piston seal carefully run-in following the instructions in section 4.5. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 26: Running-In A New Piston Seal

    If a damaged piston has been in operation, the piston seal will be destroyed and should also be replaced. To replace the piston and the Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 27: Cleaning The Inlet And Outlet Check Valves

    4 Refit the check valves. The inlet check valve (with a lip for the manifold and a larger diameter opening) is fitted to the side marked IN of the pump head. Tighten the valves until fully Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 28 See section 2.8. Note: Check valves have precision matched components and should only be disassembled further by a trained service engineer. If the problem cannot be corrected, the valve should be replaced completely. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 29: Trouble Shooting

    3 Check inlet tubing filter. It can become clogged if unfiltered buffers or samples are applied. See instructions for flushing through at the end of the run in section 3.8 Ending the run and storage . Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 30: Error Messages

    3 Switch on the instrument and check that the fans are running. 1 Switch off the instrument. ERROR Sync failure 2 Switch on the instrument. Remember to Please restart! open the purge valves before synchronization. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 31: Checking The Pump Pressure

    • partially blocked solvent filters • leaking connections • piston seal leakage • check valve malfunction • piston damaged Some examples of normal and abnormal pressure traces together with comments are shown in the following table. Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 32 • Inlet opened or blocked Flow rate: 20 ml/min Air bubbles passing through Pressure: 4.3 MPa pump heads, then the trace 0.5 MPa Recorder 1 V, 1 mm/s returns to normal Pump setup: Full Scale 10 MPa Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 33: Removing Trapped Air Bubbles From The Pump

    1 Run at 40 ml/min for P-901, or 4 ml/min for P-903. 2 Continue to run for 10 minutes, or until the air bubble is removed. 3 Change the eluent according to the description given in section 3.9. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 34: Reference Information

    Reference information Description A.1 Instrument Pump P-900 is a high performance laboratory pump for use in liquid chromatography and other applications where accurately controlled liquid flow is required. An eluent in an external vessel is drawn into the inlet manifold by the action of the pump.
  • Page 35 4 digital inputs and 4 digital outputs can be handled via the REMOTE connector. Pin 1–4 can then be used as inputs, and pin 6–9 as outputs. All input/output signals are 5 V TTL signals. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 36: Pump Head

    Outlet check valves Outlet, to mixer Outlet manifold Outlet check valve Rinse chamber inlet Inlet manifold Inlet check Inlet valve Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 37 The flexible rinsing membrane sweeps the piston and seals the rinsing system. The pump head is manufactured in titanium alloy. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 38: Pump Principle

    When a decrease in flow rate is programmed, the motor speed reduces rapidly to the lower flow rate. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 39: Outlet Manifold

    The external chart recorder output for pressure is 0–1 V. The full scale range is set under sub menu under Set Pr Full Scale Setup, Reference information B.2.2. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 40: B Menus

    4 The service contract number is displayed, press OK. 01234567 YM 012345 5 The instrument serial number is displayed, press OK. Pump P-900 V1.00 6 Instrument name and software version are displayed, press OK. Date of Maintenance 7 The date of the last service is displayed, press OK.

  • Page 41: Setup Menu

    Setup Gradient Mode Setup Gradient Mode 2 Select either , press OK. 1 2 D Note: Selecting one pump gradient mode disables the B pump module. Gradient mixing is not possible in double mode. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 42: Set Pump Type

    , press OK. Setup Mixer Setup Mixer (auto) off on auto 2 Select either , press OK. ”Auto” means the mixer is auto running when the pump is in mode. Run, Pause Hold Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 43: Set Mixer Operation

    , press OK. Setup 2 Select sub menu press OK. Set Display Angle, Set Display Angle ( ->|) ->\ ->| ->/ 3 Select viewing angle ( Mid or Down), press OK. ->\ ->| ->/ Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 44: Alarm Timer

    The instrument has service displays for use by authorised service Enter Access Code! personnel. If the service display is accidentally Enter Access Code: selected, press the ESC-button to exit to the normal operation display. Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 45: Menu Overview

    11374, 2398 Check Service Mode Telephone Service 012345678901 Calib Press Offset Contract Number 012345678901 Serial Number 01234567 YM 012345 Pump P-900 V1.00 Date of Maintenance Setup Setup Hi Press Limit Buzzer Test (10.00MPa) 8.00 Setup Lo Press Limit (0.00MPa) 10.00 Setup Pr.

  • Page 46: C Technical Specifications

    <±1% at 0.05–10 ml/min reproducibility rsd <0.25% at 0.05–10 ml/min Pressure sensor Range 0–27.5 MPa Offset error <0.05 MPa Scale error <±2% Environment +4 to +40 °C 20–95% relative humidity 84–106 kPa (840–1060 mbar) Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 47 5 V TTL low or contact closure (see section A.1 for pin significance) Digital output TTL, open collectors Recorder output 0–1 V full scale Functions Languages selectable; English, German, Spanish, French, Italian Display 2 rows with 20 characters each Pump P-900 User Manual 18-1120-04 Edition AB...
  • Page 48 Reference information Dimensions, H x W x D 150 x 260 x 370 mm Weight 17 kg EMC Standards This product meets the requirement of the EMC Directive 89/336/EEC through the harmonized standards EN 50081-1 (emission) and EN 50082-1 (immunity) Note: The declaration of conformity is valid for the instrument when it is •...

  • Page 49: D Accessories And Spare Parts

    Reference information Accessories and spare parts Item Quantity per pack Code no. P-901 Stand-alone 18-1114-00 P-901 System mounted 18-1108-56 Seal kit in4cluding 4 piston seals and 4 rinse membranes: Seal kit, 100 ml: 18-1113-12 Piston kit, 100 ml 18-1112-13 Pump head capillaries 100 ml 18-1117-52 Rinsing/draining housing 100 ml 18-1112-03...
  • Page 50 PEEK tubing, i.d. 0.50 mm, o.d. 1/16" 18-1113-68 PEEK tubing, i.d. 0.75 mm, o.d. 1/16" 18-1112-53 Teflon tubing, i.d. 0.75 mm, o.d. 1/16" 18-1112-54 PEEK tubing, i.d. 1.0 mm, o.d. 1/16" 18-1115-83 Fingertight connector 1/16" 18-1112-55 Pump P-900 User Manual 18-1120-04 Edition AB...

  • Page 52: Short Instructions On

    Short instructions The following short instructions are intended as a guide to users who are fully familiar with safety precautions and operating instructions described in this manual. The instructions assumes that the instrument is installed according to the installation instructions. P -9 Select by means of the mains...

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