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Table of Contents
Summary of Contents for OPTARC x40
- Page 1 OptArc x40 dry and x100 oil Objectives User Guide Document created: 18.09.2022, Last edited: 18.09.2022 Manual prepared by OptArc − Designers and manufacturers of custom optical and optoelectronic systems using 3D printing rapid prototyping and based in the UK. optarc.co.uk...
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Page 2: Safety Information
Risk to vulnerable groups OptArc lab supplies and equipment are not toys. They contain small parts which may come loose, glass components that may splinter or break or otherwise present a choking or sharp object hazard or chemical hazard. -
Page 3: Table Of Contents
Introduction........................6 Need for a condenser lens....................7 ............................8 ............................8 How to insert and remove a specimen for use with the x40 (dry) objective.....9 How to insert and remove a specimen for use with the x100 (oil immersion) objective.12 Maintenance........................14 Immersion medium.....................14 Cleaning........................14... -
Page 4: Download Link For Pdf Version Of This Manual
Download link for PDF version of this Manual You can download the latest version of this User’s Manual as a PDF file from the Support section of the OptArc website via this link: https://www.optarc.co.uk/support/ OptArc x40 and x100 oil Objectives Page 4 of 16 User Guide v1.0... -
Page 5: Legal Information
Only competent people trained in the use of microscopy and the handling of laboratory equipment should use this product. Dr Paul J. Tadrous, TadPath and OptArc cannot accept any liability for any loss or damages that may occur from the misuse or mis-handling of this product. -
Page 6: Introduction
These issues are also relevant regardless of the supplier of the objective lens – even if you are using lenses that are not supplied by OptArc. Also, even if you are an experienced microscopist with traditional bench top laboratory microscopes, the unique design of the PUMA microscope poses its own challenges. -
Page 7: Need For A Condenser Lens
Need for a condenser lens The x40 objective has a numerical aperture (NA) of 0.65 and the x100 oil has a maximum NA of 1.25. These objectives will, therefore, only give good quality images if they are used with a wide angled beam illumination system. Without such illumination the images will appear ‘tinny’, hypercontrasted, dark, overly refractile and lacking in... - Page 8 Epi-illumination video https://youtu.be/cAEB10K8PqI Köhler illumination video https://youtu.be/XEE-el7vC5k OptArc x40 and x100 oil Objectives Page 8 of 16 User Guide v1.0...
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Page 9: How To Insert And Remove A Specimen For Use With The X40 (Dry) Objective
Figure 2. Turn the fine (or coarse) gear in the anti-clockwise direction to raise the focus plate and lift the objective away from the specimen on the stage. OptArc x40 and x100 oil Objectives Page 9 of 16 User Guide v1.0... - Page 10 1 mm gap (see figure 3) Figure 3. Looking at the x40 objective from the side, the slide will be in focus when the working distance (the gap between the lower lens of the objective and the top surface of the coverslip) is about 1 mm, as shown.
- Page 11 (like components on a printed circuit board) – careful frequent observation from the side while moving the sample around is required. The x40 objective is a dry objective. You must not get any immersion oil or water or other substance onto the lens surface of this objective. If you are going to observe...
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Page 12: How To Insert And Remove A Specimen For Use With The X100 (Oil Immersion) Objective.12
This also gets the scope into a better par-focal situation so you will not be far out of focus if you first focus with a x40 dry objective before switching to the x100 oil. Once you have located you field of interest with the dry x40 objective, raise the focus platform by two full rotations and remove the optical tube. - Page 13 Remove the objective from the optical tube and continue to wipe clean the front surface of the objective with a lens tissue before keeping it safe in its supplied case prior to next use. See the ‘Maintenance’ chapter for more information. OptArc x40 and x100 oil Objectives Page 13 of 16 User Guide v1.0...
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Page 14: Maintenance
Maintenance Immersion medium The x40 objective must be used dry without an immersion medium of any kind. If any immersion medium gets onto the lens you should clean it off before further use. See ‘Cleaning’ below. The x100 oil immersion objective is designed to be used with only synthetic microscope immersion oil. - Page 15 18 mm diameter coverslip in a PUMA filter slider-holder as shown (inset) and keep this ‘filter’ in the lowest filter slot of your microscope. OptArc x40 and x100 oil Objectives Page 15 of 16...
- Page 16 [End of Document] OptArc x40 and x100 oil Objectives Page 16 of 16 User Guide v1.0...