Biobase BK-PR48 User Manual

Automated sample processing system

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Automated Sample Processing System

BK-PR48

User Manual

Biobase Biodustry (Shandong) Co., Ltd

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Page 1 Automated Sample Processing System BK-PR48 User Manual Biobase Biodustry (Shandong) Co., Ltd...
Page 2 +86-531-81219804 Intellectual property The intellectual property of this user manual and its corresponding products belongs to Biobase Biodustry (Shandong) Co., Ltd. (hereinafter referred to as “our company” or “us”). No individual or organization may reproduce (including photographing, reprinting, transcription, etc.), copy, modify or translate any part of this manual without the prior written consent of our company.
Page 3 Manufacturer's Statement Biobase Biodustry (Shandong) Co., Ltd (hereinafter referred to as “our company” or “us”) has the final interpretation right of this user manual. Our company is responsible for the safety, reliability and performance of the product when all of the following requirements are met: ...
Page 4 Warranty and Repair services The standard warranty period of this product is 1 year. During the warranty period, the products will have free after-sales service.However, if the product needs to be repaired due to the following reasons, it does not fall into the scope of free after-sales service.
Page 5: Table Of Contents
atalogue Foreword.............................. I Warranty and Repair services......................III Security Information........................... 1 1 Product Description..........................5 1.1 Basic Information........................5 1.2 Application Scope......................... 5 1.3 Parameters..........................5 1.4 Product Principle........................6 1.5 Packing List...........................6 1.6 Consumables......................... 6 2 Product Installation Requirements....................8 2.1 Environmental Requirements......................8 2.2 Power Supply Requirements......................
Page 6 8.3 Maintenance Before Out of Service..................41 9 Product Maintenance........................42 9.1 Cleaning Agent........................42 9.2 Daily Maintenance......................42 9.3 Monthly Maintenance......................43 9.4 Yearly Maintenance......................43 9.5 Abnormal Maintenance....................... 43 9.6 Prophylactic Measures......................43 9.7 Pollution Exclusion......................44...
Page 7: Security Information
Security Information Logos, labels and symbols Various safety symbols and tests are used in this user manual and the analyzer to remind you of the things you need to be aware of during operation. As shown in the following table: Symbol Description Biohazard:...
Page 8 Safety precautions To use this system safely and effectively, please read the following safety precautions carefully. Any operation that violates the following safety precautions may cause system damage or personal injury. If users do not follow instructions to use the system, the protective measures provided by the system may be invalid. Electric safety To use electricity safely and prevent electric shock and equipment damage, please observe the following precautions.
Page 9 Mechanical hazard To prevent mechanical hazards, please observe the following precautions. Mechanical hazard:  When the instrument is working, there is a potential danger. The operator must be professionally trained and must follow the instructions to ensure operation in a safe area. ...
Page 10 Biohazard: All liquids and solids in the laboratory are considered to be biohazardous,  and users must take general precautions in the laboratory. All clinical samples are considered to be potentially infectious. Improper use  may lead to infection. Please do not touch the samples directly with your hands.
Page 11: Product Description
1 Product Description Automated Sample Processing System（BK-PR48） is a set of high throughput, high precision, high efficiency, pollution prevention automated sampling transfer processing system developed by our company.This instrument can directly load vacuum blood collection tubes or virus collection tubes. The system integrates sample carrier, sample retention carrier, reagent carrier, pipette tip carrier, sample application arm, protection system, transportation system, and industrial control system.
Page 12: Product Principle
1, (2mL centrifuge tube+2mL Preserve tube+5mL Preserve Reagent carrier tube)4+4+4（2mL and 5mL Located separately） External droplet capture tray design Pollution prevention and control Air-tight and anti-drip design Nucleic acid extraction samples in tubes Product function Nucleic acid extraction reagent loading (develop and retain PCR system construction function) Suitable for nucleic acid HS32 and HS96 nucleic acid extraction system...
Page 13 Store the extraction PCR tubes products, the reaction liquid, etc.. 1000μL tip Liquid distribution 200μL tip Liquid distribution 50μL tip Liquid distribution 1.5mL Centrifuge Sample storage tube...
Page 14: Product Installation Requirements
2 Product Installation Requirements 2.1 Environmental Requirements  The installation environment should be free of dust, mechanical vibration, pollution, large noise sources and electromagnetic interference as much as possible.  For indoor use, avoid direct sunlight or in front of heat and wind sources, and choose a well-ventilated location.
Page 15 Figure 2-1...
Page 16: Instrument Unpacking And Installation
3 Instrument Unpacking and Installation 3.1 Check Before Unpacking After receiving the packing box, please check the outer packaging of the instrument carefully to see if there is any damage, and check if the anti-touch and anti-tilt shipment labels turn red. As shown in the figure below, if there is damage or the label turns red, please contact our company or local agent.
Page 17 1. Unpack the outer package. 2. Check the appearance of the instrument, take out the pearl cotton inside the instrument. 3. Take out the accessory box, check the packing list, and find the tool wrench from the accessory box. 4. Move the instrument to the reserved installation space. 5.
Page 18: Product Structure
4 Product Structure 4.1 External Structure The product appearance is shown in the figure below. Table 4-1 Automated sample processing system structure Name Function Notes LCD screen Show operation software interface. Power switch Power supply for control instrument. Sampling arm Used for pipetting.
Page 19 Figure 4-2 Table 4-2 The product internal structure Name Function Notes Sampling arm Used for pipetting. Tips carrier For the placement of tips. Waste tips carrier For the placement of waste tips. Reagent carrier For the placement of reagent tube. Deep well plate carrier For the placement of deep well plate.
Page 20 4.2.1 The function of the tips carrier Figure 4-2-1 Tips carrier Table 4-2-2 Structure tips carrier Name Function Notes Tips carrier For the placement of tips. Waste tips carrier For the placement of waste tips. positioning bolt Guiding groove shrapnel Figure 4-2-2 Tips carrier function: As shown in the figure 4-2-2, the tip head carrier is positioned by two guiding grooves...
Page 21 Right demonstration: Wrong demonstration: Waste tips carrier function: The waste tips box is placed at the outermost end of the tips carrier, the tips box and the carrier together are put into the the required no-tip carrier. The empty carrier will be used to place the used and waste head.
Page 22 4.2.2 The function of the reagent carrier Figure4-2-4 The reagent carrier Table 4-2-5 The structure of reagent carrier Name Function Notes Centrifuge tubes carrier For the placement of 1.5-2ml centrifuge tubes Preserve tubes carrier For the placement of 2ml preserve tubes Preserve tubes carrier For the placement of 5ml preserve tubes Position 1-4 of the reagent carrier can place 1.5-2mL centrifuge tubes, position 5-8 of...
Page 23 4.2.3 Deep well plate carrier Figure 4-2-5 The deep well plate carrier can place two kinds of deep well plates, namely "H plate" and "standard plate". When placing deep well plates, the gap of deep well plate shall be outward as shown in figure 4-2-5, and the deep well plate shall be completely placed on the carrier without warping angle, and completely parallel attached to the deep well plate carrier.
Page 24 Sample carrier area PCR tubes carrier area Figure 4-2-6 and sample carrier are placed in the same area. Two carriers cannot PCR tubes carrier coexist at the same time, only one carrier can be placed, as shown in Figure 4-2-6. The sample carrier can hold 48 samples of 5mL preserve tubes, and the PCR tubes carrier can hold 1.5 to 2ml single-row eight tubes.
Page 25 Table 4-2-9 Modular structure Name Function Notes Tips carrier installation position Install the tips carrier Reagent carrier installation position Install the reagent carrier Deep well plate carrier installation Install the deep well plate position carrier PCR tubes carrier installation position Install the PCR tubes One carrier can be carrier...
Page 26 Magnetic adsorb Figure 4-2-8 Reagent carrier installation...
Page 27: Software Description
5 Software Description Main functions of the software: 1. Software Startup 3. Program Management 5. About 2. Program Operation 4. Instrument Management 6.Exit 5.1 Software Startup Software Startup：Double-click the software startup icon 5.2 Program Operation [Program Operation]:In this interface, you can update the tips before operating the software program and operate the program.
Page 28: Program Management
5.3 Program Management Enter the login password in the following interface：“666666”. [Program Management]:In this interface includes p ,liquid parameters,, roject editor import and export etc.. Figure 5-3-1 [Project editor]：Edit and input the project parameters in this interface.
Page 29 Figure 5-3-2 [liquid parameters] ：In this interface, you can edit liquid parameters. Figure 5-3-3 Aspiration parameters  "Suction speed":The speed of suckback,the suckback action performed after suction of the pipette tip(to prevent needle tip hanging fluid)  "Maxi speed":Maximum speed of suction. ...
Page 30: Instrument Management
 "Spit volume":The volume of liquid returned after suction by the pipette tip.  "Cut-off speed":It stops when it drops to this set speed.  "Start speed":The speed at which the suction is started.  "Following speed":The speed following the liquid level when suction. Injection parameters ...
Page 31: About
Figure 5-4-2 [Sample debugging] : Debug the XYZ axis position of the sampling arm and the X-axis position of the Anti-drip plate. 5.5 About [About]: In this interface, you can view the software and hardware version numbers, operation log and manual.
Page 32 Figure 5-6 [Query]: Click query to [Query] the software and hardware version number. [Manual]: Click the [Manual] to view the operating instruction of the Automated Sample Processing System. [Run log]: Click to query the operation log records of the instrument.
Page 33: Operating Instruction
6 Operating Instruction 6.1 Experimental Process Step 1: Check before power-on ↓ Step 2: Power-on ↓ Step 3: Preparation before experiment ↓ Step 4: Standard experimental procedure ↓ Step 5: Experiment closed ↓ Step 6: Disinfection ↓ Step 7: Shutdown 6.2 Check Before Power-on Be sure to check the instrument carefully before operation.
Page 34: Standard Experimental Procedure
preserve tube or the corresponding extraction reagent instructions. 2. Draw up a plan for the consumables required by the test and the steps required by the test. 3. Please deal with the reagent according to the reagent instructions, such as thawing, mixing.
Page 35: Shut Down
 Amplification test on specified instrument (or nucleic acid extraction test on specified nucleic acid extraction). When the experiment is running： Please strictly follow the standard operating procedures and do not blindly or  continuously click. Caution It is forbidden to put materials unrelated to the experiment into the ...
Page 36 Figure 6-6-1 6.7.2 Sample allocation input Figure 6-6-2 Click the name of the sample to be edited under the task list (for example, 457 is the task to be edited) and click "457". There are four operation options in the upper right of the editing interface , select sample allocation and click [Add] in the action management bar to edit the sample allocation.
Page 37 6.7.3 Reagent distribution input Figure 6-6-3 Click the name of the sample to be edited under the task list (for example, 457 is the task to be edited) and click "457". There are four operation options in the upper right of the editing interface , select reagent distribution and click [Add] in the action management bar to edit the reagent...
Page 38 Change TIP N times: Reused numbers of tips . 6.7.4 Product distribution Figure 6-6-4 Click the name of the sample to be edited under the task list (for example, 457 is the task to be edited) and click "457". There are four operation options in the upper right of the editing interface , select product distribution and click [Add] in the action management bar to edit the product...
Page 39 6.7.5 Negative and Positive Control Figure 6-6-5 Click the name of the sample to be edited under the task list (for example, 457 is the task to be edited) and click "457". There are four operation options in the upper right of the editing interface , select Negative and Positive Control and click [Add] in the action management bar to edit the negative...
Page 40: Software Update
7 Software Update Single Step Movement Click [Instrument Management] and click "Sampling motor debugging" on the instrument management interface. Under this interface, you can control the single step movement of the adding sample arm/twisting cap arm. [Sampling arm]:After selecting target board position "...
Page 41: Cap Screwing Debugging
Figure 7-3 7.2 Cap Screwing Debugging [Registration point]: There are two options "1" and "2" after clicking, "1" is the initial bit and "2" is the termination bit, as shown in Figure 6-12. Positioning and set to "1" click mobile, screw-top arm will reach the target location, near the "x" shaft position debugging, the x axis shift to the right, for example, through debugging "x"...
Page 42 Figure 7-5 Figure 7-6 The x axis debugging Table 6-12-2 The x axis debugging Function Note The default location value is displayed The number of move steps selection X moves to the left X moves to the right Initialize the X-axis X to zero Figure 7-7...
Page 43: Sampling Arm Debugging
7.3 Sampling Arm Debugging Bottle opening Tips liquid level reference line Tips scanning bit Bottle reference line opening Bottle opening The lowest tips reference line Figure 7-8 Vertical position debugging Debug in the Y direction inward Debug in the Y direction outward...
Page 44: Software And Hardware Version Query
Debug in the X direction leftwards Debug in the X direction rightwards Tube wall Tips Figure 7-9 Correct mechanical position Figure 7-10 7.4 Software and Hardware Version Query Click [About] interface, click [Query] to query the software and hardware version number.
Page 45: Manual/Operation Log Query
Figure 7-11 7.5 Manual/Operation Log Query Click [About] interface, click "Manual" to view the operating Manual . Click [About] interface, click "Run Log" to view the peration log.
Page 46: Faults Handing
8. Faults Handing 8.1 Mechanical Arm Module Table 7-1 Failure phenomenon Possible Causes Treatment measures The sampling arm operation is Check for any obstacles. The sampling pump cannot blocked or collided. run to the set location Other reasons. Contact the technical services personnel. Reassemble the tip carrier shelf so that the The tip carrier shelf is not in place.
Page 47: Alarm And Troubleshooting
8.2 Alarm and Troubleshooting Table 8-2 Alarm prompts Reasons Treatment measures There is no needle in the needle box, but the Click "Retry". back face has a needle. Please replace the new All the sampling needle box and click needles are used out. "Retry".
Page 48: Product Maintenance
9 Product Maintenance 9.1 Cleaning Agent Specifications of cleaning agent: Cleaning Agent Specification Water Distilled or deionized water Alcohol 75% ethanol Warning: The cleaning agent Should be consistent with this instruction and the high  concentration may dissolve the carrier and the equipment surface coatings. Do not use the hypochlorite-containing disinfectant (bleach liquid and chlorine) ...
Page 49: Monthly Maintenance
remove the power outlet. The instrument is a precision instrument, the user can not dismantle the machine.  9.3 Monthly Maintenance Clean the sampling pump adapter with a tip needle,then wipe its end and outer walls with a swab dipped in alcohol. 9.4 Yearly Maintenance Clean the instrument countertop,tip carrier and the enclosure.
Page 50: Pollution Exclusion
 Wear experimental clothes, disposable gloves,and wipe away the residual liquid on the surface of the instrument with a dry and clean cloth.Clean the surface of the instrument with cloth dipped in 75% ethanol. 9.7 Pollution Exclusion To protect the maintenance personnel,the equipment must be cleaned in accordance with the standard laboratory specification (GLP) prior to repairing this automated sample processing system.
Page 51 Biobase Biodustry (Shandong)Co., Ltd Add: No.9 Gangxing Road, High-Tech Zone, Jinan City, Shandong Province, China Tel: +86-531-81219801/03 Fax: +86-531-81219804 E-mail: service_ivd@biobase.cc Web:www.biobase.cc/www.meihuatrade.com/www.biobase.com...