Cairn OptoLED Manual

Two channel led illuminator
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OPTOLED
Two channel LED illuminator
The OptoLED power supply controls up to two OptoLED heads providing bright, stable illumination across
the visible, UV and IR range via a closed-loop photodiode feedback design. With sub-millisecond, vibration
free switching and intensity modulation, it's suitable for fast, demanding imaging requirements as well as
routine fluorescence or transmitted light microscopy.
This manual provides guidance on setting up a new OptoLED system, operation via external control, and
installation and alignment on your microscope. The FuraLED and TwinLED dual LED couplings are also
included in this guide.
For the purpose of this manual, all words in
document.
1.
Important Safety Information: p.1
2.
2.1
2.2
3.
General Operation: p.4
3.1
3.2
3.3
4.
LED alignment on your microscope: p.8
4.1
4.2
4.3
4.4
1.
Important Safety Information
The OptoLED can produce very intense illumination.
The LED heads should
surface, such as a piece of paper and view the resultant reflection or fluorescence.
This is especially important with
can cause permanent damage if not used with care.
bold blue
NEVER be viewed
directly. When testing the LEDs, always shine them at a white
ULTRAVIOLET LEDs
email: sales@cairn-research.co.uk tech@cairn-research.co.uk
+44(0)1795 590140 www.cairn-research.co.uk
refer to parts labelled in diagrams throughout this
which will not appear bright to the human eye, but
M a n u a l
1

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Summary of Contents for Cairn OptoLED

  • Page 1: Table Of Contents

    This manual provides guidance on setting up a new OptoLED system, operation via external control, and installation and alignment on your microscope. The FuraLED and TwinLED dual LED couplings are also included in this guide.
  • Page 2: Setting Up Your Hardware

    Setting up your hardware The OptoLED power supply is typically supplied with two operating channels, interchangeable LED heads, an optional remote slider, coupling hardware for combining the LEDs and a suitable microscope mount. The power supplies have two identical control sections on the rear and front panels. Typically, the short- est wavelength is connected to the left channel when viewing the front of the power supply, although all LEDs will function on any channel of the OptoLED controller.
  • Page 3: Hardware Connections: Optoled Power Supply Front Panel

    Hardware connections: OptoLED power supply front panel Figure 2 : OptoLED power supply front panel Switch the red power button ON REMOTE / FRONT PANEL switch FRONT PANEL Ensure the is set to unless you are using a remote slider, in which case select REMOTE. If...
  • Page 4: Remote Slider Control (If Applicable)

    3. General Operation The OptoLED LED heads can be operated from either the controller front panel, the remote slider or externally via TTL and/or an analogue voltage. The front panel control is described in section 2.2 and is often used for continual illumination, such as checking cell transfections.
  • Page 5: Optical Feedback

    FEEDBACK When the switch is set to ‘ON’ on the OptoLED rear panel, a feedback loop uses the output of a photodiode amplifier built into the head to set the control voltage, rather than just the intensity dial or slider. LED chip manufacturers and specifications vary, and when you factor in the influence of temperature, LEDs tend to be less efficient as they get hotter.
  • Page 6 When is Feedback not useful? We would recommend switching Feedback on during general OptoLED operation, particularly consid- ering the spectral output of an LED can also change with temperature. If you are pulsing an LED with a very short duty cycle however, having Feedback enabled will limit the switching speed to ~10µs (vs.
  • Page 7: External Triggering

    3.3 External Triggering The OptoLED heads can be switched on or off, as well as the intensity being set by an external analogue voltage via BNC connections on the rear panel. We also supply a data interface as an optional extra with the system, plus the corresponding device drivers for a range of third-party acquisition software.
  • Page 8: Analogue Triggering

    3.3.2 Analogue triggering For intensity control in your software, the current provided to the OptoLED heads can be determined by an analogue voltage. Often, this is used in combination with the TTL on/off triggering, therefore the description below assumes the GATE inputs have already been determined.
  • Page 9: Single Port Coupling

    Single port coupling OptoLED Head LED Focus Adjustment LED XY Adjustment Figure 6: Single port coupling OptoLED Head Insert the into the single port coupling, taking note of the notch section on the outer casing. LED Focus Adjustment Secure the screw within this notch on the LED head –...
  • Page 10: Dual Port Coupling

    Dual port coupling When mounting two LEDs, our dual port coupling includes a Cairn filter cube, which houses a dichroic mirror (with suitable long pass characteristics) to reflect the LED of a shorter wavelength. Each LED has individual X, Y and Z adjustments to ensure optimal illumination.
  • Page 11 Figure 9: Installing dichroic mirrors and filters in Cairn filter cubes Please refer to specific guidance from your filter manufacturer for details on correct dichroic mirror and filter orientation. email: sales@cairn-research.co.uk tech@cairn-research.co.uk...
  • Page 12 Reflected LED XY adjustment (note the magnetic door can remain in place). This mechanism tips and tilts the Cairn cube housing the dichroic mirror, acting as a precise alignment tool for the reflected pathway. email: sales@cairn-research.co.uk tech@cairn-research.co.uk +44(0)1795 590140 www.cairn-research.co.uk...
  • Page 13: Triple Port / Quad Port Coupling

    Always ensure the LEDs are in wavelength order, with the shortest closest to the microscope frame and that suitable dichroic mirrors are present in the Cairn filter cubes with the shortest wavelength in the fully reflected position and the longest in the fully transmitted position. Please also ensure that suitable dichroic mirrors are fitted to the Cairn filter cubes.
  • Page 14 Cover the objective aperture with small piece of white paper, or a centring tool provided by your microscope manufacturer. Turn on the 340nm OptoLED channel at a low intensity. 340nm LED focus Using the 2.5mm hey key provided, adjust the until the image of the LED comes into sharp focus on the piece of paper.
  • Page 15 Using the 1.5mm allan key provided, ensure both LEDs are FuraLED horizontal and vertical adjustments. central using the Please contact us if you have any questions or issues during OptoLED setup or operation and we will be happy to help! tech@cairn-research.co.uk email: sales@cairn-research.co.uk tech@cairn-research.co.uk...

Table of Contents