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YeaStar
RNA Kit
™
RNA from any tough-to-lyse sample
Highlights
•
Quick,
spin-column
small/microRNAs) from a wide range of fungi species, susceptible to
yeast lytic enzyme lysis.
•
Omits the use of mechanical homogenization and organic
denaturants.
•
RNA is ready for Next-Gen Sequencing, RT/qPCR and any
downstream application, etc.
Catalog Numbers:
R1002
Scan with your smart-phone camera to
view the online protocol/video.
purification
of
total
RNA
(including
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Table of Contents
Related Manuals for Zymo Research YeaStar RNA Kit
Summary of Contents for Zymo Research YeaStar RNA Kit
- Page 1 YeaStar RNA Kit ™ RNA from any tough-to-lyse sample Highlights • Quick, spin-column purification total (including small/microRNAs) from a wide range of fungi species, susceptible to yeast lytic enzyme lysis. • Omits the use of mechanical homogenization and organic denaturants. •...
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Page 2: Table Of Contents
Table of Contents Product Contents ..........01 Specifications ..........02 Product Description ........03 Protocol ............04 (I) Buffer Preparation ......... 04 (II) Total RNA Purification ......05 Ordering Information ........06 Complete Your Workflow ....... 07 Notes ..............08 Guarantee ............ -
Page 3: Product Contents
Product Contents R1002 YeaStar ™ RNA Kit (40 prep) YR Digestion Buffer 3.2 ml YR Lysis Buffer 6.4 ml 6 ml RNA Wash Buffer (concentrate) DNase/RNase-Free Water 6 ml 1000 U Zymolyase (lyophilized) Zymolyase Storage Buffer 0.5 ml Zymo-Spin ™ IIICG Columns Collection Tubes Instruction Manual... -
Page 4: Specifications
Specifications Sample Sources – Any fungi species (1-5 x 10 • pelleted cells from 1-1.5 ml culture susceptible to yeast lytic enzyme lysis. RNA can also be isolated from aged cells grown on solid or in liquid medium. Specific sample types include, but are not limited to: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. -
Page 5: Product Description
Product Description The YeaStar RNA Kit provides for rapid isolation of total RNA from a ™ wide range of fungi species, susceptible to yeast lytic enzyme lysis including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. -
Page 6: Protocol
Protocol The protocol consists of: (I) Buffer Preparation and (II) Total RNA Purification (I) Buffer Preparation Add 24 ml 100% ethanol (26 ml 95% ethanol) to the 6 ml RNA Wash Buffer. Add 200 µl Zymolyase Storage Buffer to the lyophilized Zymolyase (final concentration 5 U/µl) and mix by gentle inversion. -
Page 7: (Ii) Total Rna Purification
(II) Total RNA Purification Perform all steps at room temperature and centrifugation at 10,000-16,000 x g for 30 seconds, unless specified. 1. Pellet 1-5 x 10 cells (1-1.5 ml culture) by centrifugation at 500 x g for 2 minutes. Then carefully remove the supernatant. 2. -
Page 8: Ordering Information
Ordering Information Product Description Catalog No. Size YeaStar RNA Kit R1002 40 preps. ™ Individual Kit Components Catalog No. Amount YR Digestion Buffer R1001-1 3.2 ml YR Lysis Buffer R1001-2 6.4 ml R1003-3-6 6 ml RNA Wash Buffer (concentrate) R1003-3-24 24 ml W1001-10 10 ml... -
Page 9: Complete Your Workflow
Complete Your Workflow For tough-to-lyse samples, use ZR BashingBead Lysis Tubes: ZR BashingBead Lysis Tubes 2.0 mm beads #S6003 Plant/animal tissue 0.1 + 0.5 mm beads #S6012 Microbes 0.1 + 2.0 mm beads #S6014 Microbes in tissue/insects For isolation of RNA from any sample: ... -
Page 10: Notes
Notes... -
Page 11: Guarantee
100% satisfaction guarantee on all Zymo Research products, or your money back. Zymo Research is committed to simplifying your research with quality products and services. If you are dissatisfied with this product for any reason, please call 1(888) 882-9682. Integrity of kit components is guaranteed for up to one year from date of purchase.
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