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Summary of Contents for molgen PurePrep 96
- Page 1 Operation Manual V1.4 PurePrep 96 Nucleic Acid Purification System...
- Page 2 Revision history Version number Description of revision Date revision Revised by V1.0 Initial release version 21-06-2019 Xu Tao Increase of the temperature rise time V1.1 23-08-2019 Xu Tao index V1.2 Addition of the U-disk accessories 15-05-2020 Xu Tao Namen instrument en adres V1.3 7-1-2021 John R.
- Page 3 Foreword Thank you for purchasing our PurePrep 96 Nucleic Acid Purification System. This manual describes the function and operation of the instrument. In order to use the instrument properly, please read this manual carefully beforehand. Keep it as reference book to solve possible difficulties you may encounter.
- Page 4 The operator should not repair the instrument in case any injury or out of warranty. If service required, please contact MolGen or your local supplier for repair. Before powering on, please make sure the voltage of the power supply is consistent with the required voltage.
- Page 5 Disconnect the power cord from the jack at once in the following cases, and contact your local supplier or MolGen: ⚫ Liquid enters into the Instrument;...
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Page 6: Table Of Contents
Table of contents Chapter 1. Introduction ...................... 6 1.1. Application ......................7 1.2. Contraindication ....................7 1.3. Service Life ......................7 Chapter 2. Specifications ....................8 2.1. Working Conditions ....................8 2.2. Basic Parameters ....................8 2.3. Overall Dimensions ................... 9 Chapter 3. -
Page 7: Chapter 1. Introduction
Some examples of matrices used are blood, cultured cells or bacteria, tissues, cell-free body fluids and plant samples. The PurePrep 96 can be used in high-throughput flows, as users can extract DNA/RNA from 1~96 samples simultaneously with various kits. -
Page 8: Chapter 2. Specifications
Chapter 2. Specifications 2.1. Working Conditions Environmental Temperature: 10C～35C Relative Humidity: ≤70% Input Voltage: AC 100～240V，50Hz/60Hz 2.2. Basic Parameters Model PurePrep 96 Parameters Principle Magnetic Particle Method, Magnet type Sample Volume 50μL—1000μL Throughput Stability CV≤5% Extraction time 10 ~ 60min/run Temperature control Ambient temperature ~ 120℃... -
Page 9: Overall Dimensions
2.3. Overall Dimensions The below figure (fig 1) shows the dimensions of the PurePrep 96. The units are in mm. Fig 1... -
Page 10: Chapter 3. Basic Operating Instructions
Chapter 3. Basic Operating Instructions This chapter mainly introduces structures, basic operation keys, displays, as well as preparations before starting up. Please read this chapter carefully before using this instrument. 3.1. Structures 3.1.1. Front Display Shortcut keys and indicator lights Cabin door Fig 1 3.1.2. -
Page 11: Cabin Door
3.1.3. Cabin Door The cabin door of the PurePrep 96 can be opened which is convenient for cleaning and maintenance. Cabin door Magnetic rod Magnetic rod’s tip Heating plate Fig 4 3.1.4. Transparent Cover The transparent cover is on the right side of the instrument, which assists in placing or taking out plates. -
Page 12: Touch Screen
3.1.5. Touch Screen Indicator light Display screen STOP Fig 6 Display screen: Touch screen, mouse also can be connected for operation. TAB: Select shortcut program. RUN: Start the shortcut program and run the instrument. STOP: Stop the operation. -
Page 13: Chapter 4. Operations
4.2. Kits Installation Open the cabin door, press the position button (figure 7) to turn the rotary table and place all the plates on their proper position of the rotary table. The PurePrep 96 is suitable for 96-well plates. Position button... -
Page 14: Detailed Operations
4.3. Detailed Operations 4.3.1. Start-up Interface Make sure the front door and the plastic side door are closed beforehand, and turn on the machine using the power button on the back of the machine. On the display screen, the start-up interface will show up (see figure 8). - Page 15 4.3.2. Run Program Interface This interface including two modes: “shortcut mode” and “list mode”, as shown in below Fig 9 and Fig Fig 9 Fig 10 In the “List mode” interface, if a program is selected/activated in “Shortcut” column, the icon of the program can be displayed on shortcut interface.
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Page 16: Run Interface
4.3.3. Run Interface In “List mode” or “Shortcut” mode, select the required program and click “Run” to enter the run interface. When starting a run, the instrument will first detect whether there is are plates on the rotary table. If no plate is found, the program will prompt to confirm whether the following steps can be continued, as shown in the figures 11 and 12 below. - Page 17 The instrument will install magnetic rod sleeves automatically. If rod sleeves are already installed on the current magnetic rod sleeve rack, "Sleeve loaded, continue?” will pop up (see figure 13). If no magnetic rod sleeve is detected after installing the magnetic rod sleeve, "No sleeve, continue?" will appear (see figure 14).
- Page 18 After the magnetic rod sleeve is successfully installed, the instrument automatically performs the following steps (see figure 15). Fig 15 In the running interface, users can stop, pause, continue or run the program again. Plate number 1 (dark blue in figure 15) is the working plate, the red corner mark on it means the plate is running or already finished running, while the blue corner mark means the plate is ready to run.
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Page 19: View
4.3.4. View Select the required program (in the list mode or shortcut mode) and click the “View” button to enter the view interface (see figure 16). Users can view all parameter settings of the program. Fig 16 Users can click the button in the upper right corner to switch to the graphic display. - Page 20 Click the button to open the magnetic parameter absorption interface which shows the magnetic parameters of the selected step (see figure 18). Clicking the button “Option” will display the settings of the program (see figure 19). Fig 18 Fig 19...
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Page 21: Manage Program
4.3.5. Manage Program Users can manage all programs in the “Manage Prog.” interface. Fig 20 4.3.5.1. Management Interface The management interface is similar to the list interface in program operation, except that the lock column is a non-operable option in the program run interface while it’s an operable option in the management interface. - Page 22 If you create a new program, or double-tap an existing program, it will show all the existing steps in the program. Double-tapping on a step gives you the display as seen in figure 21, where you can double- tap on each step to change settings. Fig 21 In this screen, you can alter parameters per step.
- Page 23 Fig 22 When you enter the overview of the parameters in magnetic absorption, you can alter several settings. It is not advised to alter these, as any change can have a significant effect on operation efficiency. Only trained personnel can alter these settings. The parameters are explained below: •...
- Page 24 4.3.5.3. Option In the interface of creating or editing a program, click the “Option” to enter the option interface (seen in figure 23). The parameters displayed are applied to the whole program. Fig 23 The following buttons and parameters are explained: •...
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Page 25: System Settings
4.3.6. System Settings In the “Settings” interface, “Instrument”, “Date&time”, “Language”, “Air ejector fan”, “Import&export” and “Upgrade” can be tapped to modify their respective settings (see figure 24). In the following chapters, the separate menus are elaborated upon. Fig 24 4.3.6.1. System Date and Time Tap the “Date &... - Page 26 4.3.6.2. Language Settings For language there are two options: Chinese and English (see figure 26). Select the desired language and press “OK“ to save the modification. Fig 26 4.3.6.3. Fan In this menu, the air ejector fan can be turned off (see figure 27). Fig 27...
- Page 27 4.3.6.4. Import and Export In the “Import&export” menu, the below display (figure 28) is shown. Fig 28 Press the "Import " to enter the USB directory where you can select the program required. Then press the "Ok" to import. Press the "export" button to enter the system directory where you can select programs and then "Ok" to export files to the U disk.
- Page 28 4.3.6.6. Operation Record Each run of the program automatically generates a running record. These can be seen in the “Log” interface (see figure 30). Fig 30 Users can find records of specific runs by using the “Search” button as seen in figure 31. Logs can be exported through the export key.
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Page 29: Lighting
4.3.6. Lighting At the right bottom of the screen, the icon indicates the lighting is on. Users can click the icon to switch between turning the light on and off. 4.3.7. Auxiliary function The Plate position switch function and the sleeve automatic installation function can be used with this software. -
Page 30: Uv Sterilization
4.3.8. UV Sterilization The UV sterilizer interface is mainly used for the opening and closing of the UV lamp. The time can be set by pressing “+” or “-” button. The program can automatically determine half of the set time to sterilize the half circle of the rotary table, with a minimum of 2 min, as shown in the figure below. -
Page 31: Chapter 5. Trouble Shooting
Chapter 5. Trouble Shooting 5.1. Troubleshooting Symptom Cause Further actions Power not connected Check power Switch failure Replace switch No display after turning the system on Fuse failure Replace fuse (5X20 250V 8A) Other Contact with Supplier Replace light tube, otherwise No UV light UV light failure contact with supplier... -
Page 32: Software Error Alarm List
5.2. Software error alarm list Fault type Fault name Error T1 Overheat E011 Temperature (code: 0) T1 Open circuit E015 T1 Short circuit E016 Baffle motor sensor E404 Rotary motor sensor damaged E405 Lifting platform motor sensor damaged E406 Electric machinery stroke position (code:4) Push rod motor sensor damaged... -
Page 33: Chapter 6 Accessory
Chapter 6 Accessory Name Specs. Unit Qty. Remark Power cord Mouse Logitech For upgrading software USB disk and transferring programs... -
Page 34: Chapter 7. Abbreviations And Tags
Chapter 7. Abbreviations and Tags 7.1. Abbreviations The following abbreviations are for reference and will appear in this operation manual. ampere alternating current volt Hertz watt universal serial bus secure digital card WiFi wireless Fidelity kilogram millimeter μL microliter hectopascal ℃... -
Page 35: Tags
7.2. Tags The following marks will appear on the instrument Warning label Heating label Conformité Européenne, CE-marking Be careful of hands, injury warning... - Page 36 Notes...
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