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Table of Contents
Summary of Contents for molgen PurePrep 32
- Page 1 Operation Manual V1.0 PurePrep 32 Nucleic Acid Purification System...
- Page 2 Foreword Thank you for purchasing the PurePrep 32 Nucleic Acid Purification System. In order to use the instrument properly, please read carefully this manual before operating and keep it for future reference. Opening Check Please check the instrument and Appendix with the packing list when you first open the package.
- Page 3 Safety Warnings and Guidelines 1 Warning Please read this Manual carefully before operation. Operation without reading the manual may cause damage or even electrical shock. 2 Safety Tips The operation, maintenance and repair of the Instrument should comply with the basic guidelines and cautions as below.
- Page 4 During operation, the surface temperature of heating block inside operation window could be very high. To avoid possible scald or boiling of the liquid, do not touch the metal part when operating. The Instrument should be placed in a position with low humidity, less dust, and keep it away from water, sunshine and strong light source.
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Page 5: Table Of Contents
2. Connect the power ....................... 6 3. Install the reagent kit ....................7 Install the 96 well reaction plate on PurePrep 32........... 7 4. Install the magnetic rod cover ..................8 Install the magnetic rod cover for PurePrep 32 ............8 5. - Page 6 UV sterilization ..................... 22 Help ........................23 Chapter 5 Troubleshooting ..................24 Chapter 6 Spare Parts List ..................26 1. PurePrep 32 Spare parts list ..................26 Chapter 7 Abbreviation and Symbols ..............27 1. Abbreviation ......................27 2. Symbols ........................28...
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Page 7: Chapter 1 Brief Introduction
The PurePrep 32 Nucleic Acid Purification System uses the magnetic rod to adsorb, transfer and release magnetic particles to transfer the sample to purify DNA/RNA, protein and cell etc. The PurePrep 32 is able to handle 1 to 32 samples simultaneously with extraction kits defined for different matrices. -
Page 8: Chapter 2 Specifications
Specifications 1. Normal operating conditions Environmental temperature: 10C～35C Relative Humidity ≤70% Input: AC 100～240V，50Hz/60Hz 2. Basic parameter & Performance Table 1 Basic parameter & Performance Model PurePrep 32 Parameter Magnetic Bead Method Principle Throughput 1~32 Kits 96 Deep-well plate Sample 50~600 Volume/μL... -
Page 9: Overall Dimension
3. Overall Dimension Dimension（W×D×H） PurePrep 32： 400mm×470mm×450mm... -
Page 10: Chapter 3 Product Introduction
Chapter 3 Product Introduction This chapter basically introduces the instrument structure, operation buttons, display panel and the preparations before operation. When using this instrument for the first time, please make sure to read this chapter before start. 1. Structure 1.1. Front Display screeen Shortcut key... -
Page 11: Operation Panel
PurePrep 32 Inside view Magnetic rod UV light (Over the magnetic rod cover) (top inside) Magnetic rod cover stand Heating strip Drawer (bottom inside) Note: The only difference between the experiment module of above products is the shape of the heating strip. -
Page 12: Chapter 4 Operation
Chapter 4 Operation 1. Preparation Take out the instrument from the packing carton and tear off the tape on the edge of the operation window, then open it and take out the foam. First please screw out the fixed screw of position ① as below .Second please screw the screw of position ② then you can take out the baffle and release the moving component. -
Page 13: Install The Reagent Kit
3. Install the reagent kit 3.1 Install the 96 well reaction plate on PurePrep 32. Take out the drawer and put the 96 well reaction plate which is already filled with samples mounted on the locating slot, make sure the plate with chamfering should be on the left side, and then push back the drawer slowly. -
Page 14: Install The Magnetic Rod Cover
PurePrep 32 Magnetic rod cover Note: Magnetic rod cover for PurePrep 32 at most is 4pcs. 5. Remove magnetic rod cover Press the button and take out the magnetic rod cover as per below photo. -
Page 15: Operation
6. Operation 6.1 Start-up Interface Switch on the instrument and make sure the drawer is closed before start, the start-up screen will appear on the display. After start, it will enter into the shortcut mode as below: ─ ─... -
Page 16: Shortcut Mode
The program can also be selected by press “Tab” button on the panel and then press “Run” for start or “Stop” for terminate PurePrep 32 Running interface On the left side, current step info is shown. When the exact reagent position starts to run, then this position will be highlighted, and there will be temperature display on the position of heating function. - Page 17 Click “Pause” to pause or continue the program. Click “Stop”, the program will stop and there will be check box. Click “Cancel”, program will be continue and click “OK” the program run will be back to original place and the “Stop”...
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Page 18: List Mode
6.2.1 List mode Users may review the file by scroll bar on the right side. Select the program and click "Run" to enter into the run interface. Click “View” to enter into the check interface. ─ ─... -
Page 19: Lamp
Click “Run” to enter the running interface. Click “Option” to view the parameter setting of the program. Click “Back” to previous interface. 6.2.2 Lamp At the bottom of interface, the icon “ ” shows that the lamp is on, and the icon “ ”... -
Page 20: Shortcut Operation
6.3.1 Shortcut operation Click “√” of the program in the “Manage Prog.” interface, the program will be displayed in the “Shortcut” list interface. In the list of “Lock”, if the icon is “ ”, the program can’t be edited, deleted and saved as; if the icon is “... - Page 21 Click “Insert” to add a new step. Click “Well” to insert the well number, then input the program name, waiting time, mixing time, magnetic time and sample volume. At the end, please click the mixing speed to select the speed ─...
- Page 22 For PurePrep 32, only well location 1 & 6 (Corresponding to the well location of 1,6,7 &12 for 96 well plate ) have heating function. “Temperature” number box is available to input the temperature value that would like to set.
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Page 23: Program Management--Edit
6.3.3 Program Management--Edit Choose the program in the “Manage prog.” interface, then click “Edit” enter into edit program. Same step as “Insert” in 6.3.2 6.3.4 Program Management-Save as Under the “Manage prog.” interface to make selection, Click “Save as” and then input a new program name, click “Enter”... -
Page 24: System Setting-Instrument Setting
6.4.1 System Setting-Instrument Setting Click “Instrument” to input the right password and then enter the setting interface to set the parameter of instrument. Remarks：Regularly, there’s no need to set unless it’s failed and need repair, as instrument has already finished setting before factory dispatch, and even if it’s failed, this kind setting will be authorized by distributor or manufacturer. -
Page 25: System Setting -- System Time
6.4.2 System Setting -- System Time Click “Date & Time” to set system time by directly enter into number or click “+” “-”. 6.4.3 System Setting--Language Click “Language Setting” to choose the language that you need. ─ ─... -
Page 26: System Setting - Fan
6.4.4 System Setting – Fan Click “ Air Ejector Fan” to have the fan setting 6.4.5 System Setting -- Import/Export Click “Import/Export” and insert U disk to finish the step. ─ ─... -
Page 27: System Setting--Software Upgrade
6.4.6 System Setting--Software upgrade Click “Software upgrade” to input the right password enter the interface and then insert the U disk to operation. ─ ─... -
Page 28: Uv Sterilization
6.4.7 UV sterilization Click “UV sterilization” and input number or click “+” “-”to set time. Click “Start” to open the UV light to start UV sterilization and time count down. Click “Stop” to stop the UV sterilization. During sterilization, the UV light will automatically stop when the drawer is open, and it will continue after the drawer is closed. -
Page 29: Help
6.5 Help Click “Help” to check the help info.Help interface displays the relevant features and version information. ─ ─... -
Page 30: Chapter 5 Troubleshooting
Chapter 5 Troubleshooting Error Possible Causes Solution Power not connected Check power Switch failure Replace switch No display after switch on Replace fuse （5X20 250V 8A） Fuse failure Others Contact with Distributor Replace light tube No UV light UV light failure Contact with distributor Replace light tube No light... - Page 31 Software alarm list Alarm type Error name Error message T1,T2,T3,T4,T5,T6, E011,E021,E031,E041, T7,T8 Overheat E051,E061,E071,E081 T1,T2,T3,T4,T5,T6, E018,E028,E038,E048, T7,T8 Drive circuit fault E058,E068,E078,E088 Temperature T1,T2,T3,T4,T5,T6, E015,E025,E035,E045, (code: 0) T7,T8 Open circuit E055,E065,E075,E085 T1,T2,T3,T4,T5,T6, E016,E026,E036,E046, T7,T8Short circuit E056,E066,E076,E086 The drive circuit of exhaust fan fault E019 The drive circuit of cooling fan fault E009...
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Page 32: Chapter 6 Spare Parts List
Chapter 6 Spare Parts List 1. PurePrep 32 Spare parts list Item Unit Remark Power line Mouse Allen wrench For upgrading software and transferring programs ─ ─... -
Page 33: Chapter 7 Abbreviation And Symbols
Chapter 7 Abbreviation and Symbols 1. Abbreviation Abbreviation used Ampere Alternating current Volt Hertz Watt Universal Serial Bus Secure Digital Card WiFi WLAN Kilogram Millimeter μL Microlitre Hectopascal °C Degree Centigrade Coefficient of variation of well Switch Operation STOP Stop ─... -
Page 34: Symbols
2. Symbols Symbols used on device Warning Heating Indicates conformity with health, safety, and environmental protection standards for products sold within the European Economic Area ─ ─... - Page 35 Notes ─ ─...
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