Summary of Contents for ChromaCon Contichrom CUBE 30
- Page 1 Contichrom System Manual Contichrom® CUBE 30/100 (FPLC) Contichrom® CUBE Combined 30/100 (FPLC) Contichrom® HPLC 30/100 System Manual page 1 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 2: Table Of Contents
Methods to fill one Inlet of a pump only ......................30 2.7. Method for capillary filling and system storage ..............31 Method Fill_Capillaries_with_Buffer_A.mth ....................31 Method Valves_Storage_Position.mth ......................31 page 2 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 3 Calibrating the pH sensor ....................48 6.3. Pump Maintenance ......................50 Pump seal wash Solution Replacement ......................50 Pump Head Maintenance ..........................50 Cleaning the Pump Head Assembly ........................51 page 3 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 4 CaptureSMB®, N-Rich®, AutomAb®, ChromIQ® are all registered trademarks of ChromaCon AG. The absence of a product or service name or logo from this list does not constitute a waiver of ChromaCon AG's trademark or other intellectual property rights concerning that name or logo.
-
Page 5: Safety Symbols / Symboles De Sécurité
NOTICE indicates a situation which, if not avoided, could result in damage to the instrument. It needs to be ensured that the situation is resolved before proceeding. page 5 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 6: Regulatory Information
The Contichrom system platform comprises the following components: Contichrom system according to the table below Laptop with the ChromIQ software installed Optional Fraction Collector Auxiliaries page 6 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 7 The eluate from the first column is directly loaded on the e second column whereby inline dilution can be used. 2C-PCC (CaptureSMB) increases throughput and decreases resin consumption in affinity capture applications compared to single column batch capture chromatography. page 7 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 8: Contichrom System Illustration
The following illustration is representative of Contichrom CUBE Combined 30/100 system and of Contichrom HPLC 30/100 system: Contichrom CUBE pH electrode Contichrom CUBE USB and LAN connection to Computer Fraction collector computer CUBE+ upgrade module page 8 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 9: The Contichrom System
GENERAL INSTRUMENT OVERVIEW The Contichrom platform comprises the following main components: Contichrom CUBE module (30/100), present in Contichrom CUBE 30/100 systems, Contichrom CUBE Combined 30/100 systems and Contichrom HPLC 30/100 systems CUBE+ upgrade module (30/100), present in Contichrom CUBE Combined 30/100 systems and in the Contichrom HPLC 30/100 systems The CUBE+ upgrade module, in combination with the CUBE module, forms the “Contichrom CUBE Combined... - Page 10 Side and back view of the CUBE module (top) Power switch Socket for Power cable Connector for link to CUBE+ module Side and back view of the CUBE+ module (bottom) page 10 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 11: Overview Of Wetted System Components
Flow cell for fixed dual wavelength detector, conductivity, temperature Buffer selection valve for P2 WARNING: Contichrom equipment must not be used with human contagious materials and/or radioactive materials. page 11 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 12: Liquid Fluid Path
Flow Sheet of the Contichrom CUBE Combined 30/100 systems (pump seal wash lines not shown). The flow sheet for the Contichrom HPLC 30/100 systems are identical: page 12 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 13: Twin Column Fluid Paths
Contichrom System Manual Flow Sheet of the Contichrom CUBE 30/100 systems (pump seal wash lines not shown): WARNING: Be aware that protruding capillaries and tubes may trap parts of the body or clothing causing abrasion and/or injury. 1.3.2. TWIN COLUMN FLUID PATHS The Contichrom systems have been designed to support both single column batch chromatography and chromatography processes with two columns. -
Page 14: Adding And Removing Tubes
Contichrom System Manual The following schematic illustrates the fluid paths for Contichrom CUBE 30/100 and Contichrom CUBE Combined 30/100 systems// Contichrom HPLC 30/100 systems used in the different single and multicolumn processes. Contichrom CUBE Combined 30/100, Contichrom HPLC 30/100 Contichrom CUBE 30/100 1.3.3. -
Page 15: Instrument Components
The Contichrom CUBE 30/100 modules are provided with two high precision double-head pumps (P2 and P3), each equipped with a buffer selection valve on the suction side (VP2, VP3). In the Contichrom CUBE 30/100 system, P2 and P3 are referred to as PA and PB, respectively, by the ChromIQ operating software. The pumps can operate independently in isocratic mode or may be joined to run a gradient. -
Page 16: Back Pressure Regulators
For these columns, the backpressure regulators 2.-4. (see above) must be removed. The backpressure regulator on the drain outlet 1. (see above) must never be removed! page 16 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 17: Valves
The pumps P2 and P3 of the Contichrom CUBE Combined 30/100 system and Contichrom HPLC 30/100 system (pumps PA and PB of the Contichrom CUBE 30/100 system) are equipped with automatic buffer selection valves on the suction side. Each buffer selection valve can select among 8 different buffer/feed solutions (see illustration below). - Page 18 P2 inline dilution by P2 From P2 CIP outlet From P2 CIP outlet Inlet plugged/ store Waste outlet Inlet plugged/ store Waste outlet position position page 18 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 19: Detectors
Make sure that the secondary drain of the fraction collector routes liquid from the drip tray of the fraction collector to a safe collection point. page 19 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 20: Preparing The Contichrom Instrument
2.1. STARTING THE SYSTEM The Contichrom CUBE 30/100 system is switched on by plugging the power cable in and pressing the power switch. Contichrom CUBE Combined 30/100 systems and Contichrom HPLC 30/100 systems are switched on by plugging the power cables of the CUBE and the CUBE+ modules and by pressing their power switches. - Page 21 In addition, a fraction collector window will be shown in case a fraction collector is connected to the Contichrom system. Screen shot of the main window (right) and the fractionation window (left) page 21 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 22: Buffer/Solvent Preparation
WARNING: Do not fill buffers overhead due to risk of spillage WARNING: Use a suitable, approved and safe aid to step-on when placing buffer containers into the buffer tray on the Contichrom system. page 22 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 23: Buffer Tank Assignment And Tank Levels
The virtual tanks should be updated whenever the real tanks are refilled in order to display the correct filling levels and to trigger warning messages when appropriate. page 23 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 24 A set of tank settings can be saved (8.) and loaded (7.) at a later stage. page 24 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 25 (66 mL – 50 mL = 16 mL). If there is sufficient buffer available, the missing amount is zero and a green square is displayed. page 25 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 26: Preparing The Fluid Outlets
Screenshot of the procedure tab. The content of every single tank, an error level and a warning level can be set and the tanks can be assigned to pump inlets. The fraction collection vessels should be large enough to accommodate the selected fraction volumes. page 26 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 27: Filling The Pump Seal Wash
Make sure that the inlets are fully submerged in the washing solution. (see schematic below). Place a syringe with blunt needle in one of the outlets and draw liquid out the air and liquid into the line. page 27 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 28: Filling The Pumps
Pumps P2 and P3 (corresponding to pumps PA and PB of the CUBE 30/100 system) can have multiple buffers connected to them on the suction side through the buffer selection valves. In order to prime each of the inlets, page 28 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 29: Standard Methods For Priming Pumps
(P1A) or inlet 1 (P2, P3), respectively. The last step sets all pumps are set to a flow rate of 0 mL/min. Contichrom CUBE 30/100: This method primes the inlets on the low pressure side of the pumps P1A, P1B (inlets 1-5). -
Page 30: Method "Load_Buffers_7-Positions.mth
(P1A) or inlet 1 (P2, P3), respectively. The last step sets all pumps are set to a flow rate of 0 mL/min. Contichrom CUBE 30/100: This method primes the inlets on the low pressure side of the pumps P1A, P1B (inlets 1-7). -
Page 31: Method For Capillary Filling And System Storage
This method performs an auto zero on both UV detectors. The total duration of the method is approximately 3 sec. METHOD BACKFLUSH_DETECTORS.MTH NOTICE: Before running this procedure, please remove the columns! page 31 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 32: Other Methods
Contichrom System Manual This method back-flushes the UV detector using the buffer on pump P2 (pump P1A in Contichrom CUBE 30/100), inlet 1. It is used to remove persisting air bubbles from the detector. Each flow cell is flushed for 30 sec at 10 mL/min top-down and subsequently for 30 sec at 10 mL/min bottom-up. -
Page 33: Creating Methods And Performing Runs
Columns of outer diameter of > 10 mm have to be mounted using a separate stand and clamps (see below). Union pieces (see picture below) should be used as placeholders when no columns are used and when system storage methods are carried out. page 33 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 34: Mounting The Ph Flow Cell And Electrode
Contichrom system. The pH electrode is mounted on the flow cell by means of a screw ring provided with the electrode. The pH electrode should point upwards. page 34 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 35: Preparing The Fraction Collector
For 15 mL centrifuge tubes choose the setting “18 mm tubes (N=72)”, for 50 mL centrifuge tubes select “25/28 mm vials (N=36)”. page 35 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 36: Operation In Cold Cabinet Or Cold Room
300 cm/h. The calculations for the feed requirement are based on a run duration of 5 cycles for 2C-PCC (CaptureSMB), N-Rich and MCSGP. Using 10 cm bed height is page 36 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 37: Preparing A Procedure To Be Run
A 10 cm bed may be assembled by connecting 2x 5 cm columns in series with a low dead volume connection. Recommended column sizes for Contichrom CUBE 30, Contichrom CUBE Combined 30 and Contichrom HPLC 30 Process... -
Page 38: Procedure For Loading Buffers
“experiment name”. In the ChromIQ measurement results folder structure, entering new names will generate a new folder with the project name and a new subfolder with the experiment name. Alternatively, existing project and experiment names can be used. page 38 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 39: Managing A Run
Screenshot of the ChromIQ control bar. Since a procedure is already running “start procedure” and “continue” are disabled. An example for the full ChromIQ user interface including menu bar, tab register and control bar is shown below: page 39 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 40: Refilling Buffers During A Run
During a run, the warnings “low buffer” or “buffers empty” may be displayed. While the “low buffer” warning is displayed, the pumps continue to run. While the “tank empty” warning is displayed, the system is in freeze mode. page 40 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 41: Monitoring A Run
V5). There is also a connection between V2B and V1A and from V1B to the waste outlet (“weak”) Screenshot of the Flowsheet tab. (Contichrom CUBE Combined 30/100 and Contichrom HPLC 30/100) page 41 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 42 30/100 only). The drain multiposition valve V6 can direct the flow of the pumps P2 and P3 (PA and PB for Contichrom CUBE 30/100) into the system (position 1), or to the drain (position 2), see screenshot below. The “Curves”-tab of the ChromIQ user interface shows the online signals and the traces of the currently running method.
-
Page 43: Programming Methods
A new Log Book file is created each day that the system is in use and the complete daily log is saved to the folder C:\Contichrom\log books. page 43 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 44: Cleaning The System
(UV, conductivity, pH). Extraordinary cleanings should be performed at product/project changeover, before storing the system for a longer period (weeks) or before relocating the system. page 44 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 45 14. Leave the system in 20% Acetic Acid for 15-30min 15. Insert all inlet tubing into a bottle filled with at least 2L of de-ionized water page 45 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 46: Storing The System
Also turn off the CUBE+ module using the power switch on the side if present. NOTICE: When keeping the Contichrom system in a cold room or cold cabinet, leave it switched on permanently to avoid condensation. page 46 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 47: Storing The Ph Electrode
WARNING: Before performing maintenance on parts of the Contichrom system on the outside of the sheet metal case, turn off the Contichrom modules and unplug the power cables page 47 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 48: Recommended Maintenance Intervals
“calibration” tab is not visible, check the box saying “Calibration tab hidden” to display the calibration tab (see screen shot below). Make sure to have three calibration solutions ready (typical calibration solutions are pH 4.0, pH 7.0 and pH 10.0). page 48 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 49 3. Repeat step 1. for the third calibration solution Afterwards confirm and save the calibration. Rinse the pH electrode and mount it according to chapter 2.13. page 49 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 50: Pump Maintenance
Wear protective gear. Removing the Pump Head Assembly The self flushing pump head assembly is shown in the figure below. page 50 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 51: Cleaning The Pump Head Assembly
Figure: Self flushing pump head assembly To remove the pump head: 1. Turn OFF the power of the Contichrom system modules (one module for Contichrom CUBE 30/100, two modules for Contichrom CUBE Combined 30/100 and Contichrom HPLC 30/100). 2. Unplug the power cords of the CUBE/CUBE+ modules. -
Page 52: Replacing The Pump Head
CAUTION: Using any other “tool” will scratch the finish of the sealing surface and create a leak 4. Repeat the procedure for the low-pressure seal in the flush housing. 5. Inspect, and if necessary, clean the pump head as described above. page 52 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 53: Replacing The Seals
2. For Pump P1 there are two channels A, B. Make sure to run in the seals belonging to the channel on which maintenance has been performed page 53 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 54: Pistons
Then, run the pump for 15 minutes at a back pressure and flow rate listed under PHASE 2 below, depending on the pump head type. Contichrom CUBE 30, Contichrom CUBE Combined 30, Contichrom HPLC 30: PHASE 1 Pump Type... -
Page 55: Check Valve Cleaning And Replacement
When installing new check valves, notice the outlet has a transparent washer, and the Inlet has a cross ball retainer. Also, the words INLET and OUTLET should be visible on the top of the self-flush check valves. (see figure below): page 55 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 56: Seal Wash Check Valve Replacement
Note: The Sapphire Seat is an opaque white ring. The red ruby ball can be seen through the ring. Flow is always away from the sapphire seat, as shown by the directional arrows etched on the capsules. page 56 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 57: Valve Maintenance
The only possible maintenance operations are the exchange of the rotor and the exchange of the stator. When exchanging the stator the rotor should be exchanged as well. NOTICE: page 57 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 58: Cleaning And Rotor Replacement
3. Test the valve by pressurizing the system. If it doesn’t hold pressure, contact technical service for repair. Tightening pattern of the valve stator screws page 58 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 59: Replacing Tubing Connections
1. Tight seal short connection In order to mount the tube, first slide the fitting and then the nut over the tubing as indicated above. page 59 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 60 The mounting of the tubing for the buffer selection valves is in analogy to the mounting of the tubing for the pump inlets (see above). Note that the fittings are colored and the nuts are transparent for the buffer inlet valves. page 60 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 61: Detector Maintenance
CHROMIQ SOFTWARE UPDATE Check for ChromIQ operating software updates every six months with the Service support. You will be contacted by Service in case critical updates are required. page 61 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 62: Troubleshooting
Contichrom hardware Adjust firewall settings / turn off firewall Check if the system components are found by starting the system center. The system page 62 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 63 (typically in the range of minutes up to a few hours) cyclic runs typically may be resumed without having the freeze mode affect the performance of the run for more than the current cycle. page 63 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 64 The pump used to produce the flow is not working properly see pump troubleshooting The UV1 or UV2 cell is dirty or the flow path is dirty page 64 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 65 provide constant temperature environment Calibration is required Check calibration with a standard solution of known conductivity page 65 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 66 Check calibration with a standard solution of known pH Perform conductivity calibration if necessary pH electrode broken / expired Replace pH electrode page 66 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 67 Inlet check valves are blocked flow. Based on the pressure signal, identify the affected pump. Clean or replace inlet check valves of that pump (see section on pump maintenance) page 67 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 68 Changes become effective for the next sample being collected. Fraction collector powered off or not connected Check the power and network connection of the fraction collector page 68 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 69 Wrong rack type selected Enter correct rack type in fractionation window Fractionator does not move forward Fractionator ignores “NEXT” command Turn fractionator on / off again page 69 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 70: Disposal Of The System
7.4. SYSTEM QUALIFICATION TESTS For priming the system and to test functionality of the individual components, refer to the Contichrom system qualification document. page 70 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 71: Appendix
Technical specifications: Contichrom CUBE 30 system / Contichrom CUBE 100 system for FPLC The Contichrom CUBE 30 system / Contichrom CUBE 100 system comprises the Contichrom CUBE module / Contichrom CUBE 100 module, respectively. Technical specifications: Contichrom CUBE module / Contichrom CUBE 100 module for FPLC ... - Page 72 PTFE,1/8”,1.6 / 2.0 mm i.D.(CUBE+ 30 /CUBE+ 100) Fittings: PEEK Operation Cold room (4C) compatible 2 buffer inlets (1 per pump) page 72 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 73 Operation Cold room (4C) compatible 16 buffer inlets (8 per buffer selection valve) ® Operating software ChromIQ operating software (in English) description page 73 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
- Page 74 PTFE,1/8”,1.6 / 2.0 mm i.D.(CUBE+ 30 /CUBE+ 100) Fittings: PEEK Operation Cold room (4C) compatible 2 buffer inlets (1 per pump) page 74 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 75: Appendix B - List Of Wetted Parts
Some materials used in the Contichrom system are extremely sensitive to acids (including some Lewis acids) and acid halides. System cleaning with any amount of hydrochloric acid should be avoided. page 75 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 76 Aqueous and nonaqueous solutions containing free hydrofluoric acid (HF) cannot be allowed to make contact with the flow cell, even intermittently. HF is a weak acid and will form in any acidic solution which contains page 76 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...
-
Page 77: Appendix D - Room Requirement For Operation And Storage
Maximum Power consumption: Contichrom CUBE module: 90 W Contichrom CUBE 100 module: 90 W CUBE+ module: 80 W CUBE+ 100 module: 80 W page 77 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 78: Appendix E - List Of Spare Parts
USB 2.0 Cable 880452CC PEEK Check Valve Capsules, 10 Pack 890370 Pump Head Kit (1 req'd per pump head) 890371 Pump Self-Flush Kit (1 req'd per pump head) page 78 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... -
Page 79: Appendix F - Manual Control Using System Center
[mL/min]). The red button “Q=0” immediately sets the flow rate to zero. The small grey button next to the “Q=0” button starts/resets a counter [mL], show on the right side of the grey button. The buffer page 79 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com... - Page 80 The data generated using the System Center may be recorded by clicking the “Record Data” button (9.). The data file name and path is displayed below the “Record Data” button. The System Center is closed using the “Close” button (10.) page 80 of 80 Contichrom® System Manual v2.6 / 07/2017 © ChromaCon AG www.chromacon.com...