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Alpha MED Scientific MED64 MED-C11 Product Manual

Med multi-well connector
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The most sensitive microelectrode array system 
for in vitro extracellular electrophysiology
Product Manual
MED Multi-well Connector
P/N: MED-C11

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Summary of Contents for Alpha MED Scientific MED64 MED-C11

  • Page 1 The most sensitive microelectrode array system  for in vitro extracellular electrophysiology Product Manual MED Multi-well Connector P/N: MED-C11...
  • Page 2 © 2016 Alpha MED Scientific Inc. All rights reserved. Version: 1.00; April 1, 2016 Alpha MED Scientific Inc.

  • Page 3: Table Of Contents

    Contents 1. MED Multi-well Connector Components and Functions 1-1. Components and their functions ..............1 2. Installation 2-1. Connecting the MED Multi-well Connector to the MED64 Head Amplifier ..3 2-2. Running the MED64-Allegro System and checking the noise ......4 3. Instruction for use 3-1.
  • Page 4 Contents MED64 Product Manual - MED Multi-well Connector (MED-C11)

  • Page 5: Med Multi-Well Connector Components And Functions

    1. MED Multi-well Connector Components and Functions 1. MED Multi-well Connector Components and Functions The MED Multi-well Connector (MED-C11) is the Connector for the MED64-Allegro System. It connects the MED Multi-well Probe to the MED64 Head Amplifier (MED-A64HE1S) allowing recording extracellular signals from 4 (or 8) cultured samples in a MED Multi-well Probe.
  • Page 6 1. MED Multi-well Connector Components and Functions Accessories (1) MED Connector Cable (20 pins) -4 sets- (2) Grounding wire (3) Test-board Figure 3. Accessories for the MED Multi-well Connector. (1) MED Connector Cable (4 sets) Connects the Output terminals to the INPUT terminals on the MED64 Head Amplifier (MED-A64HE1S). (2) Grounding wire Connects the Grounding terminal to the SIGNAL GND of the MED64 Head Amplifier (MED-A64HE1S) for grounding.

  • Page 7: Installation

    2. Installation 2. Installation 2-1. Connecting the MED Multi-well Connector to the MED64 Head Amplifier • Read product manuals for all other components of the MED64-Allegro System prior to installation. 1. Place the MED Multi-well Connector on a stable table and AWAY from any equipment which generates an electric or magnetic filed (e.g.

  • Page 8: Running The Med64-Allegro System And Checking The Noise

    2. Installation 2-2. Running the MED64-Allegro System and checking the noise Install the Test board to the MED Multi-well Connector and check the noise. The baseline noise should be around several microvolts at all 64 channels as seen in the Figure 6. If noise is larger than a few microvolts, check the followings: 1.

  • Page 9: Instruction For Use

    3. Instruction for use 3. Instruction for use 3-1. Installing the MED Multi-well Probe onto the MED Multi-well Connector 1. Clean the MED Multi-well Probe with kimwipe soaked with 70% ethanol before sliding into the Connector. • The leads in the MED Multi-well Probe contact with gold pins of the MED Multi-well Connector. (Refer to Figure 15 in the page 11) Salt residue, or even finger print can cause the contact pins to rust or accumulate residue build up.

  • Page 10: Electrode Orientation In The Med Multi-Well Connector

    3. Instruction for use 3-2. Electrode orientation in the MED Multi-well Connector The Figure 9 and 10 shows the electrodes’s orientation when the MED Multi-well Connector is installed with its terminal oriented towards the right side. Each well in the MED Multi-well Probe has 8 (or 16) reference electrodes as well as 16 (or 8) recording electrodes, those are connected independently to the terminal.

  • Page 11: Med 8Well Probe [Med-P5N801 / Med-P5N811]

    3. Instruction for use MED 8well Probe [MED-P5N801 / MED-P5N811] Figure 10. Electrode orientation for the MED-P5N801/811 (8 well) in the MED Multi-well Connector and terminal assignment. Product Manual - MED Multi-well Connector (MED-C11)

  • Page 12: Running The Med64-Allegro System

    3. Instruction for use 3-3. Running the MED64-Allegro System Signals acquired through the MED Multi-well Connector is displayed in the Mobius as following: 4 Well Probe 8 Well Probe Channel # Well # Channel # Well # Channel # Well # Channel # Well # Ch1‐16 Ch33‐49 Ch1‐8 Ch33‐40 Ch17‐32 Ch50‐64...

  • Page 13: Stimulation With The Med64-Allegro System

    3. Instruction for use 3-4. Stimulation with the MED64-Allegro System The MED64-Allegro System allows you to stimulate each well sequentially. Use Mobius Acquire MED64R2 Data w/Stim’s Step1-8 to stimulate one (or 2 channels) per well (sample) sequentially. Inactivate the Step 5-8 when the 4 Well Probe is used. Set the Trace interval (stimulus interval) for longer than 5 seconds since the amplifiers need at least 5 seconds to recover after stimulation.

  • Page 14: Troubleshoot

    4. Troubleshoot 4. Troubleshoot When noise appears during your experiments, it is important to identify the source of the noise. Likely reasons are: • Damaged electrodes of the MED Probes • Uncleaned and/or damaged contact pins in the Connector. • Loosely connected the cables. •...

  • Page 15: Maintenance

    5. Maintenance 5. Maintenance 5-1. Cleaning of the contact pins The 64 leads of a MED Multi-well Probe connect to the Connector via small contact pins. Clean the gold contact pins with following procedure if you observe noise from dirty pins. Figure 15.

  • Page 16: Sterilization

    6. Cautions 5-2. Sterilization Wipe the Multi-well Connector with a lint-free cloth soaked in 70% ethanol, and allow to dry. CAUTION: • Do NOT autoclave as it may damage the MED Multi-well Connector. 5-3. Storage Store in a cool dry area. Avoid exposure to high temperatures or humidity. 6.

  • Page 17: Terminal Assignment

    It does NOT cover damage incurred during shipment or problems which are caused by products not supplied by Alpha MED Scientific. In addition, this warranty does not cover problems resulting from alteration, accident, misuse, neglect, faulty installation, maladjustment of user controls, improper maintenance, modifications or service by anyone other than AMS or damage attributable to acts of God.

  • Page 18: Specifications

    9. Specifications 9. Specifications Connector unit MED Probe securing mechanism Slide in Out put 20-pin MDR system connector x 4 Material Aluminum (Gold for contact pins) Weight 700g 210 x 83 x 34 (mm) Dimensions MED Connector Cable Plug 20-pin MDR Plug cover Metal Length...
  • Page 19 MED-C11...
  • Page 20 April 1, 2016 Alpha MED Scientific Inc. Manufactured by Alpha MED Scientific Inc. Saito Bio-Incubator 209, 7-7-15, Saito-asagi, ©2016 Alpha MED Scientific Inc. Ibaraki, Osaka 567-0085, Japan Phone: +81-72-648-7973 FAX:+81-72-648-7974 http://www.med64.com support@med64.com...

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