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Columbus Instruments Exer 3/6 Hardware Manual

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Treadmill Hardware Manual
June 8, 2022
1011-0002 v1.02
950 North Hague Avenue
Phone: (614) 276-0861
Columbus, Ohio 43204-2121 USA
Fax: (614) 276-0529
Sales:
sales@colinst.com
Toll Free: 1-800-669-5011
Service:
service@colinst.com
http://www.colinst.com

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Summary of Contents for Columbus Instruments Exer 3/6

  • Page 1 Treadmill Hardware Manual June 8, 2022 1011-0002 v1.02 950 North Hague Avenue Phone: (614) 276-0861 Columbus, Ohio 43204-2121 USA Fax: (614) 276-0529 Sales: sales@colinst.com Toll Free: 1-800-669-5011 Service: service@colinst.com http://www.colinst.com...

  • Page 3: Table Of Contents

    Treadmill Hardware Manual 1011-0002 v1.02 Table of Contents Treadmill Types ............................... 5 Exer 3/6 Treadmill ..............................6 1 – System Overview ............................6 System Specifications .......................... 6 1.2 Treadmill Stimulus Detection Overview ....................8 2 – Hardware Setup ............................. 8 2.1 Item Inventory ............................
  • Page 4 Treadmill Hardware Manual 1011-0002 v1.02 5.1 Treadmill Belt Tracking ..........................31 5.2 Treadmill Belt Tension ..........................32 5.3 Cleaning ..............................32 Modular Treadmill ..............................32 6 – System Overview ............................32 6.1 System Specifications ..........................32 6.2 Treadmill Stimulus Detection Overview ....................34 7–...

  • Page 5: Treadmill Types

    Treadmill Hardware Manual 1011-0002 v1.02 10.1 Treadmill Belt Tracking .......................... 59 10.2 Treadmill Belt Tension ........................... 59 10.3 Removal of Shocker Assembly ......................59 10.4 Cleaning ..............................59 Treadmill Types Exer 3/6 Treadmill: Modular Treadmill:...

  • Page 6: Exer 3/6 Treadmill

    Exer 3/6 Treadmill 1 – System Overview The Columbus Instruments Exer 3/6 Treadmill is an economical treadmill designed for general purpose animal exercising. The basic system consists of two separate units: the Treadmill Controller, and the Treadmill. The Treadmill can have up to 6 lanes.
  • Page 7 Treadmill Hardware Manual 1011-0002 v1.02 1.1.2 Optional Air-Puff Stimulus Stimulus Pressure: 0-2069mmHg (0-40PSI), 19-liter (5-gallon) reservoir typical Stimulus Nozzles: Six per Mouse Lane, Ø 1.4mm x 13.9mm (Ø 0.055in x 0.545in) Stimulus Duration: 200mS Stimulus Repetition Rate: 1, 2, or 3Hz Stimulus Control: 2-position toggle switch (on/off) for each lane The INTENSITY adjusts the relative sensitivity of the capacitive touch...

  • Page 8: Treadmill Stimulus Detection Overview

    This ensures that quarantined control subjects receive the same stimuli as the test subjects within an experiment session. The Exer 3/6 in “Rat” mode can support only two channels yoked: 1 & 2. The “Mouse” mode can support all six channels: 1 & 2, 3 & 4, 5 & 6.

  • Page 9: Assembly

     1 x Calibration Wand  1 x Instruction Manual  1 x Treadmill Software Flash Drive (if using with Oxymax)  1 x Exer 3/6 Treadmill (shown with optional shock grid)  1 x Treadmill Lid  1 x Feces Tray ...
  • Page 10 It is recommended to use an AC power strip to provide a common access to AC power for all components of the Treadmill system and host computer. 2.2.1 Treadmill Assembly The Exer 3/6 Treadmill is about 90% assembled. • Remove any protective plastic film that may cover pieces of the treadmill.
  • Page 11 This completes the assembly of the Exer 3/6 Treadmill. 2.2.2 Downhill Running Adapter If the Exer 3/6 Treadmill came with the Downhill Running Adapter, then the system will contain the following:  1 x Right leg with rubber foot.  1 x Left leg with rubber foot.
  • Page 12 Treadmill Hardware Manual 1011-0002 v1.02 • Using one screw and nut, connect the two braces together in the middle. Do not tighten. The screw and nut should hold the braces closely together but allow them to move. • Using four screws with nuts, connect the legs and the braces together in the orientation as shown. Again, do not tighten.
  • Page 13 Treadmill Hardware Manual 1011-0002 v1.02 • Insert the inclination Rod through the hole in the front of the treadmill. Then lower the front so the treadmill rests on the Downhill Adapter feet and the Inclination Rod • Tighten all remaining screws and nuts of the legs and braces. This completes the assembly of the Downhill Adapter.
  • Page 14 Treadmill Hardware Manual 1011-0002 v1.02  Teflon tape or Teflon pipe sealant  15mm (9/16in) Open-End Wrench or Adjustable Crescent Wrench WARNING!!! Place the protective caps over the open ports of air-fittings when not in use. To setup the Air-Puff Stimulus, do the following: •...
  • Page 15 Connect the 2-circuit valve power cable between the “Valve Power” port of the Treadmill Controller and the 2-position Molex Mini-Fit Jr jack on the Exer 3/6 Treadmill. Be sure the locking lever snaps down to secure the cable to the ports.

  • Page 16: Connecting Components

    Treadmill Hardware Manual 1011-0002 v1.02 2.2.4 Electric Stimulus The stimulus grid comes pre-attached to the treadmill. To connect the stimulus grid, go to SECTION 2.3. 2.2.5 Setting Angle of Inclination or Declination The angle of inclination is set by the location of the spring pin in holes of the Inclination Rod. The holes range from 0°...
  • Page 17 Use the fasteners built into the cable ends to secure the cable to the ports. • Connect the motor cable between the “Motor” port of the Treadmill Controller and the 6-position motor cable jack on the Exer 3/6 Treadmill. Be sure the locking lever snaps down to secure the cable to the ports. •...
  • Page 18 Treadmill Hardware Manual 1011-0002 v1.02  Treadmill Stimulus Detection with AC power cord  1 x CAT5 network cable, 0.3m (1ft), 25-pin  1 or more detection cables, 1.8m (6ft), 25-pin  1 x Instruction Manual To connect the Treadmill Stimulus Detection to the Treadmill Controller: •...

  • Page 19: Treadmill Controller User Menu

    • Connect the Treadmill Stimulus Detection to the same source of AC power as the Treadmill Controller. To Connect the Treadmill Stimulus Detection to the Exer 3/6 Treadmill: • Using a detection cable, connect the “To Treadmill” port of the Treadmill Stimulus Detection to the 25- pin male D-SUB port on the back side of the treadmill.

  • Page 20: Restore Defaults

    3.2 Serial Number This option is used to see the electronic serial number which should match the Columbus Instruments serial tag on the outside of the cabinet. For service, this number may be referenced to lookup any specific details or...

  • Page 21: Run-Time Meter

    Treadmill Hardware Manual 1011-0002 v1.02 3.3 Run-Time Meter This option is used to report the total amount of time the Treadmill Controller has been powered-on. The format is Days : Hours : Minutes (DHM). The run-time meter is saved every ten minutes or when the AC power is switched off.

  • Page 22: Autocal Speed

    Verify the connection to the treadmill, if all appears correct try cycling the AC power and start the calibration again. If that doesn’t work, contact Columbus Instruments for help. Once a speed output has been detected, the low-speed calibration will automatically begin.

  • Page 23: Operating Mode

    Treadmill Hardware Manual 1011-0002 v1.02 • Acceleration Step: 0.0 to 25.5 meters/minute • Acceleration Interval: 0 to 255 seconds • End Speed: 0.0 to 102.3 meters/minute The Ending Speed ( ) is the target speed to accelerate or decelerate towards and maintain once achieved. The Acceleration Rate ( ) is determined by dividing the Acceleration Step (speed) by the Acceleration Interval (time).

  • Page 24: Calibrate Shock

    Treadmill Hardware Manual 1011-0002 v1.02 • Seconds: 0 to 59 seconds The minimum time duration is 1 second. 3.10.3 Distance Limited Mode: Dist Limit S 5.0 L 100.0 100.0m A 1.0/1 E102.3 This mode limits the distance travelled in the exercise session. This is indicated by the label “L” for the distance duration which counts down as the treadmill belt moves.

  • Page 25: Exit

    Treadmill Hardware Manual 1011-0002 v1.02 • Adjust the INTENSITY knob to achieve the necessary AC current on the ammeter. Note the reported intensity as it can vary due to manufacturing tolerances from different assembled systems. • Press the MODE button to toggle the treadmill belt mode back to STOP and return to the User Menu. The electric stimulus will be switched OFF.

  • Page 26: Stand Alone General Exercising Without The Pc

    Treadmill Hardware Manual 1011-0002 v1.02 4 – Stand Alone General Exercising without the PC 4.1 Stand Alone Initialization Attention! Use the following procedure to prevent the application of an undesirable treadmill belt speed or high intensity shock upon the application of AC power. •...

  • Page 27: Controls

    Treadmill Hardware Manual 1011-0002 v1.02 In the Time Limit Mode (indicated by “T”), the odometer is replaced by a timer which is preloaded to the duration specified in the User Menu In the Distance Limit Mode (indicated by “L”), the odometer is preloaded to the distance duration specified in the User Menu •...

  • Page 28: Treadmill Stimulus Detection

    Treadmill Hardware Manual 1011-0002 v1.02 When the treadmill belt mode is set to RUN, the treadmill belt is enabled to move, and the speed can be continuously adjusted by the SPEED knob. The display will report the set speed and the mode indicator will be a progress bar that grows from left to right.
  • Page 29 Treadmill Hardware Manual 1011-0002 v1.02 • Number of Measured Exhaustions • Time-Score Percent • Visit-Score of Visits • Repetition Rate • Begin Experiment The CHANNEL knob is used to navigate the list of parameters. Clockwise rotations will move the selection arrow up, and counterclockwise rotations will move the arrow down.
  • Page 30 Treadmill Hardware Manual 1011-0002 v1.02 4.5.6 Max_NOS Maximum Number of Stimuli: For humane reasons, the presentation of the stimulus can be stopped when the subject has received a set number of stimuli. The range is from 0 to 999. When set to zero, the presentation will not stop, and the total number of stimuli received will be recorded.

  • Page 31: Maintenance

    Treadmill Hardware Manual 1011-0002 v1.02 • Press the ODOMETER RESET button on the Treadmill Controller to initialize the odometer to zero or load the duration values. • Then press the MODE button accordingly to set the treadmill belt in motion. 4.5.13 Experiment: Data View Once the experiment has started, the screen will display the Data View.

  • Page 32: Treadmill Belt Tension

    Modular Treadmill 6 – System Overview The Columbus Instruments Modular Treadmill is an economical treadmill that can be controlled via computer interface or manually. It is designed as a stand‐alone system capable of exercising one to four subjects simultaneously. The basic system consists of three separate units: the Treadmill Controller, the motor assembly, and the treadmill running lane.
  • Page 33 Treadmill Hardware Manual 1011-0002 v1.02 Angle of Inclination: -10°‐25° in 5° increments Maximum Shock Voltage: 163V, 200ms pulse Maximum Shocker Current: 0.34‐1.6mA Enclosure Dimensions ‐ Rats: 546 x 139 x 203mm (21.5 x 5.5 x 8in) Enclosure Dimensions ‐ Mice: 384 x 51 x 102mm (15.1 x 2 x 4in) Running Surface Dimensions ‐...

  • Page 34: Treadmill Stimulus Detection Overview

    USB 2.0 Type “B” Jack 6.2 Treadmill Stimulus Detection Overview The Columbus Instruments Treadmill Stimulus Detection is designed to work with the electrical or air-puff stimulus. The Treadmill Stimulus Detection will monitor the stimuli presented to each channel (lane) and the calculate various data outputs from them.

  • Page 35: Assembly

    Treadmill Hardware Manual 1011-0002 v1.02  1 x CI-Bus Hub or CI-Bus Switch Mini  1 x USB-A to USB-B cable, 1.8m (6ft)  1 x RJ-45 communication cable, 1.8m (6ft)  Treadmill Controller with power cord  1 x Motor Cable, 2m (6ft) ...
  • Page 36 Treadmill Hardware Manual 1011-0002 v1.02 7.2.1 Treadmill Assembly • Place two of the Socket nuts into the two upper holes of one of the base plates. • Attach two standoffs to the socket nuts. Then line up another base plate with the standoffs and attach using another set of socket nuts.
  • Page 37 Treadmill Hardware Manual 1011-0002 v1.02 • Place the support bar in the third slot from the bottom, on the front of the running lane base. Place base next to motor assembly. Do not attach yet. • Set the running lane onto its base.
  • Page 38 Treadmill Hardware Manual 1011-0002 v1.02 • Attach the running lane assembly to the motor assembly by lining up the holes in the motor assembly to the standoffs and the coupler between the running lane assembly and the motor assembly. Slide the assemblies together with the coupler around both shafts.
  • Page 39 Treadmill Hardware Manual 1011-0002 v1.02 • To add extra lanes, start with the base assembly right before attaching it to the motor. • Using two more socket nuts, attach one of the additional lane base plates.
  • Page 40 Treadmill Hardware Manual 1011-0002 v1.02 • Go back to the bottom set of holes and using two more socket nuts attach two more standoffs, this time screwing the studs into the empty end of the standoffs. • Now, attach another lane base plate using another set of standoffs. Place the support bars in the third slot from the bottom, on the front of the running lane base.
  • Page 41 Treadmill Hardware Manual 1011-0002 v1.02 • Set the running lanes next to each other and attach using the coupler. • Set the running lanes onto the base.
  • Page 42 Treadmill Hardware Manual 1011-0002 v1.02 • Line up the running base with the motor assembly. Line up the standoffs with the holes in the motor assembly and the running lane with the coupler. Attach both assemblies together. Tighten coupler screws and socket nuts. •...

  • Page 43: Connecting Components

    Treadmill Hardware Manual 1011-0002 v1.02 • Insert appropriate stimulus boxes and front cap. 7.2.2 Setting Angle of Inclination The angle of inclination can be increased by moving the support bar to the next slot. The slots range from -10°-25°, in 5° increments. 7.3 Connecting Components •...
  • Page 44 Treadmill Hardware Manual 1011-0002 v1.02 • Connect one end of the shocker cable to the D type connector on the side wall of the shocker box, and the other end of the shocker cable to one of the D type connecters marked “To Shockers” located on the rear of the control unit.
  • Page 45 Treadmill Hardware Manual 1011-0002 v1.02  Treadmill Stimulus Detection with AC power cord  1 x CAT5 network cable, 0.3m (1ft), 25-pin 25-conductor male-male  1 or more detection cables, 1.8m (6ft), 25-pin.  1 x Instruction Manual To connect the Treadmill Stimulus Detection to the Treadmill Controller: •...
  • Page 46 Treadmill Hardware Manual 1011-0002 v1.02 • Connect the Treadmill Stimulus Detection to the same source of AC power as the Treadmill Controller. To Connect the Treadmill Stimulus Detection to the Modular Treadmill: • Using a detection cable, connect the “Detection” port of the Treadmill Stimulus Detection to the 25- pin male D-SUB port on the back of the treadmill lane most close to the motor assembly.

  • Page 47: Treadmill Controller User Menu

    Treadmill Hardware Manual 1011-0002 v1.02 WARNING! The stimulus detection ports on the Modular Treadmill are keyed and not interchangeable. The input port is on the left and the daisy-chain output port (for the next lane) is on the right. 7.3.2 Connecting Components with Oxymax Substitute the Oxymax CLAMS chambers with the metabolic modular treadmill lanes to use the treadmill for metabolic studies.

  • Page 48: Restore Defaults

    8.2 Serial Number This option is used to see the electronic serial number which should match the Columbus Instruments serial tag on the outside of the cabinet. For service, this number may be referenced to lookup any specific details or notes about your specific Treadmill and Controller.

  • Page 49: Fan Voltage

    Treadmill Hardware Manual 1011-0002 v1.02 8.5 Fan Voltage This option is used to set the output voltage of the Fans ports. If fans are installed on the treadmill system, the fan speed (by applied voltage) can be adjusted to provide necessary ventilation. The list of voltages are as follows: •...

  • Page 50: Acceleration Settings

    Treadmill Hardware Manual 1011-0002 v1.02 • Ensure the Treadmill Controller front panel toggle is set to STOP • Place speed sensor on treadmill belt and securely fasten with screw nut in position shown • Using the dial on the Treadmill Controller front panel, adjust the speed to 88.7 meters/min •...

  • Page 51: Calibrate Electric Stimulus

    Treadmill Hardware Manual 1011-0002 v1.02 • Normal: no limits are employed; the treadmill belt must be manually stopped. • Time Limit: a count-down timer will automatically stop the belt. • Distance Limit: a count-down odometer will automatically stop the belt. 8.10.1 Normal Mode: Normal...

  • Page 52: Exit

    Treadmill Hardware Manual 1011-0002 v1.02  AC ammeter (low range 0-10mA)  Alligator test leads for the AC ammeter  Alligator jumper wire  Fixed external resistance (animal body equivalent, 10,000 ohms typical, if used) Enable the stimulus for the mouse lane 1 or 6 by setting the toggle switch on the treadmill toward the lane. Install the alligator test leads into the appropriate current-measuring terminals on the AC ammeter.

  • Page 53: Stand Alone General Exercising Without The Pc

    Treadmill Hardware Manual 1011-0002 v1.02 8. Press and hold the ENTER button. 9. Then press and release the RESET button. 10. Release the FUNCTION and ENTER buttons. User Menu The screen will display: And then position you at the top of the list of options in the menu: ➢...

  • Page 54: Loading Subjects

    Treadmill Hardware Manual 1011-0002 v1.02 Release the RATE and MODE buttons. Use the INTENSITY knob to navigate the menu options. Details on each menu option is in SECTION 8. 9.3 Loading Subjects • Turn the clips that secure the lid and fold the lid on top of the cover. •...
  • Page 55 Treadmill Hardware Manual 1011-0002 v1.02 Simply replace the animals with the next group and press the ODOMETER RESET button. The duration counter will be reloaded and automatically the treadmill belt mode will RUN again. 9.4.2 Speed / Mode Turning the SPEED knob will adjust the set speed of the treadmill belt when its mode is set to STOP or RUN. Pressing the MODE button in the following ways will change the treadmill belt mode: •...

  • Page 56: Treadmill Stimulus Detection

    Treadmill Hardware Manual 1011-0002 v1.02 9.4.4 Motor Overload The power supply for the motor is protected by a thermal resettable fuse. If the motor is mechanically overloaded and/or demands excess power from the supply, then the thermal fuse will open. In this situation, !!! OVERLOAD !!! the motors speed will drop dramatically and the message “...
  • Page 57 Treadmill Hardware Manual 1011-0002 v1.02 9.5.2 Session No This option is not necessary but kept for backwards compatibility with previous models which directly connected to a printer for outputting the results of an experiment. If desired, the parameter can be set over the range of 0 to 99.
  • Page 58 Treadmill Hardware Manual 1011-0002 v1.02 period can range from 1 to 180 seconds. The percentage range is from 0 to 99%, 50% is typical. When set to zero, the presentation of stimulus will not stop, and the current time-score percentage will be recorded. 9.5.10 VS_Visits Visit-Score Visits: The presentation of stimulus can be stopped when exhaustion has been detected by measuring the number of visits to the detection grids over a specified rolling period in seconds, The period can...

  • Page 59: Maintenance

    Treadmill Hardware Manual 1011-0002 v1.02 9.5.14 Experiment: Setup View Pressing the FUNCTION button will toggle the screen between Data View and Setup View. This screen allows reviewing of the experiment parameters while an experiment is running. Use the CHANNEL knob to review all the experiment settings.
  • Page 60 Treadmill Hardware Manual 1011-0002 v1.02 Clean the inside of the exerciser with a mild soap solution. When cleaning the lids used with metabolic measurement be sure not to expose the fan to the mild soap solution.

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