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Related Manuals for GeneReach POCKIT
Summary of Contents for GeneReach POCKIT
- Page 1 USER MANUAL Manufacturer: GeneReach Biotechnology Corporation TEL: 886-4-24639869 / FAX: 886-4-24638255 No. 19, Keyuan 2nd Road, Central Taiwan Science Park, Taichung City 407, Taiwan www.genereach.com E-mail: sales@genereach.com / Website: 2017/03...
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Page 2: Table Of Contents
CONTENT Symbols ............................1 Section 1. Introduction ........................2 1.1 Intended Use ......................... 2 Section 2. General Information ...................... 3 2.1 Product Components ......................3 2.2 Specifications ........................3 2.3 Testing Capacity ........................3 2.4 Equipment and Materials Required But Not Provided............3 2.5 Front View .......................... -
Page 3: Symbols
Symbols Conformité Européenne EU authorized representative Supplementary fuse Grounded conductor Date of manufacturing Manufacturer Catalogue number Serial number Alternative current Caution, consult instruction for use This product is manufactured in compliance with the European Union’s Waste Electrical & Electronic Equipment (WEEE) WEEE symbol Directive 2012/19/EU. -
Page 4: Section 1. Introduction
1.1 Intended Use POCKIT is intended to provide qualitative detection of nucleic acid targets using fluorescence- based iiPCR reagents. Intended users are properly-trained laboratory technicians familiar with molecular biology techniques, and those who want to perform iiPCR or its relative reagent kits produced by GeneReach. -
Page 5: Section 2. General Information
- 200 l and 1000 l micropipette, tips, and 1.5 ml tubes - R-tubes and Caps ™ - cubee mini-centrifuge - Disposable gloves *Note: To avoid power failure, connecting POCKIT to an uninterrupted power system (UPS) and/or voltage regulator is highly recommended. -
Page 6: Front View
Releases the heat generated during reaction. A touchscreen where users key in the operation commands Control panel and view the results. A slot where users insert the SD card to run POCKIT and SD card slot save reaction raw data. Holder... -
Page 7: Rear View
2.6 Rear View ① ② Item Description Main switch To turn on/off the main power Socket To connect to the power cord 2.7 Software Version ① Item Description Software Version Current software version on POCKIT system. -
Page 8: Section 3. Important Notes
Check the device upon receiving. If any items are missing or harmed, please contact your local distributor or GeneReach immediately for replacement. Do not use any damage items as they may lead to poor performance of the test or harm the user. -
Page 9: Section 4. Operation
Section 4. Operation 4.1 Switch On Plug in the POCKIT and switch on the main power located in the back. The instrument will perform a self-test. *Note: If self-testing runs longer than 15 minutes, please see Troubleshooting. Caution: Make sure the power plug is connected to a grounded receptacle to prevent electrical shock or fire. - Page 10 2) “System Ready” will be shown after choosing the detection wavelength. 3) Before the reaction starts, to change the detection wavelength, press “CH Select” and choose again.
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Page 11: Set Up Date And Time
4.3 Set up Date and Time 1) Press “Time Setting”. 2) Touch the numbers that needs to be changed on the screen. Press “Up” and “Down” buttons to select the desired number. Press “OK” to confirm and return to the “System Ready” screen. Press “Cancel”... -
Page 12: Run Iipcr Reaction
Caution: Should any of the circumstances below occur, the results are unreliable: The lid is opened or the SD card is removed during the POCKIT reaction. The ambient temperature is not in the range of 15-35°C during the reaction. - Page 13 4) When the reaction complete, a beeping tone is made from POCKIT. Press “Buzzer OFF” to turn off the sound. *Note: If there is no beep sound after the completion of the reaction, please see Troubleshooting. 5) If the reaction temperature is abnormal during the reaction, “Temperature Warning” will be shown on the screen.
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Page 14: Display The Result
3) Description of the results: Results Description Positive Negative Undetermined Warning *Note: Please interpret the results according to the user manual of the kits used. *Note: When you see a " " symbol, please contact your local distributors or GeneReach for assistance. -
Page 15: Review The Raw Data
4.6 Review the Raw Data 1) Insert the SD card on a PC or Mac. Find the raw.csv file in the folder. 2) Please refer to the kit manual for data interpretation. 4.7 Exit the Experiment 1) Press “Abort” to pause the reaction. 2) Press “Yes”... -
Page 16: Errors Occurred During The Operation
4.8 Errors Occurred During the Operation If any Errors listed below are shown, please see Troubleshooting. -
Page 17: Switch Off
4.9 Switch Off After the reaction, switch off the main power and transfer the SD card to a PC or Mac to read the raw data. Main power Caution: Ensure the reaction has been completed before shutting down the device. -
Page 18: Section 5. Cleaning And Maintenance
Use only clean and wet paper towel or cotton swab to wipe off stains in the reaction chamber. Do not disassemble the POCKIT for cleaning as it will void the warranty. Do not use solvent, detergent or bleach to clean the POCKIT surface. -
Page 19: Troubleshooting
Troubleshooting Observation or Problems Comments and Suggestions (a) Self test runs more than 15 minutes Re-start the device. Contact your local distributors or GeneReach for assistance. (b) System crash Re-start the device, prepare new reagents in R-tube, ... -
Page 20: Reference
Reference Chang, H. G., Tsai, Y., Tsai, C., Lin, C., Lee, P., Teng, P., et al. (2012). A thermally baffled device for highly stabilized convective PCR. Biotechnology Journal, 7(5), 662-666. doi: 10.1002/ biot.201100453 Tsai, Y., Lin, Y., Chou, P., Teng, P., Lee, P., et al. (2012). Detection of white spot syndrome virus by polymerase chain reaction performed under insulated isothermal conditions.
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