Page 1 Applied Biosystems Veriti Thermal Cycler ™ User Guide...
Page 3 Applied Biosystems Veriti Thermal Cycler ™ User Guide...
Page 4 © Copyright 2008, 2010 Applied Biosystems. All rights reserved. General Laboratory Use. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, IN- CLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
Page 5: Table Of Contents
Connecting to a Network Printer ....... . . 2-5 Connecting Together Multiple Instruments ......2-5 Veriti™ Thermal Cycler User Guide...
Page 6 Thermal Cyclers ..... 3-2 ™ Disposables for the 384-Well Veriti Thermal Cycler ....3-4 ™...
Page 7 XL PCR Run Method ......... . . C-7 Index Veriti™ Thermal Cycler User Guide...
Page 8 Veriti™ Thermal Cycler User Guide...
Page 9: Preface
Guide using and maintaining the Applied Biosystems Veriti Thermal Cycler. Audience This guide is intended for novice and experienced Veriti Thermal Cycler users who use the instrument to perform PCR. Assumptions This guide assumes you have knowledge of basic laboratory procedures and PCR chemistry.
Page 10: How To Obtain More Information
“How to Obtain Support” page viii. Note: Obtaining The Veriti Thermal Cycler has a Help system that describes how to use each feature Information from of the user interface. Access the Help system by touching (Help) at the bottom the Help System right corner of the instrument touchscreen.
Page 11 If payment is not included with the package, Alpha Technics bills you for Note: $150.00 plus a 10% administrative fee. The total for the purchase order is then $165.00. 5. Send the package freight prepaid to Alpha Technics at the address in step Veriti™ Thermal Cycler User Guide...
Page 12 Preface Veriti™ Thermal Cycler User Guide...
Page 13 Physical Hazard Safety ..........xvii Safety and Electromagnetic Compatibility (EMC) Standards ....xvii Veriti™ Thermal Cycler User Guide...
Page 14: Safety And Emc Compliance Information
Safety and EMC Compliance Information Safety Conventions Used in This Document Safety Alert Four safety alert words appear in Applied Biosystems user documentation at points Words in the document where you need to be aware of relevant hazards. Each alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a particular...
Page 15: Symbols On Instruments
Safety Symbols The following table describes the safety symbols that may be displayed on Applied Biosystems instruments. Each symbol may appear by itself or with text that explains the relevant hazard (see “Safety Labels on Instruments”...
Page 16: Safety Labels On Instruments
Indicates the presence of moving parts and to proceed with appropriate caution. Environmental The following symbol applies to all Applied Biosystems electrical and electronic Symbols on products placed on the European market after August 13, 2005. Instruments...
Page 17: General Instrument Safety
ATTENTION Parties mobiles. General Instrument Safety PHYSICAL INJURY HAZARD. Using the instrument in a manner not specified by Applied Biosystems may result in personal injury or damage to the instrument. Moving and PHYSICAL INJURY HAZARD. The instrument is to be moved...
Page 18: Chemical Safety
Each time you receive a new MSDS packaged with a hazardous chemical, be sure to replace the appropriate MSDS in your files. Obtain MSDSs The MSDS for any chemical supplied by Applied Biosystems is available to you free from Applied 24 hours a day. To obtain MSDSs: Biosystems 1.
Page 19: Chemical Waste Safety
Use properly configured and approved line cords for the voltage supply in your facility. Plug the system into a properly grounded receptacle with adequate current capacity. Overvoltage The Veriti Thermal Cycler has an installation (overvoltage) category of II, and is Rating classified as portable equipment Physical Hazard Safety Moving Parts PHYSICAL INJURY HAZARD.
Page 20 Safety and EMC Compliance Information U.S. and The Veriti Thermal Cycler has been tested to and complies with standard: Canadian Safety UL 61010A-1/CAN/CSA C22.2 No. 1010.1-92, “Safety Requirements for Electrical Standards Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements.”...
Page 21 About Folders ........... . 1-15 Veriti™ Thermal Cycler User Guide...
Page 22: Chapter 1 System Overview
Veriti Thermal Cycler. This user guide provides instructions for all four models of the Veriti Thermal Cycler. Functions which are not the same for each model are indicated by a comment such as “This function is only available for the Veriti 96- Well Thermal Cyclers”.
Page 23: Using The Touchscreen
Chapter 1 System Overview Detailed specifications for the Veriti Thermal Cycler are found in Appendix A, “Instrument Specifications.” Using the Touchscreen You interact with the Veriti Thermal Cycler using a touchscreen. Table 1 describes several buttons common to many of the touchscreens.
Page 24 Enter punctuation or other symbols Symbols, then the symbol(s). When you are done, touch Symbols again. Remove the contents of the entire field Delete the last letter Close the editor and save any changes Veriti™ Thermal Cycler User Guide...
Page 25 Colons (for entering times) and dashes (for entering dates) are also automatically added. Delete the last number Remove the entire contents of the field Close the editor and save any changes Veriti™ Thermal Cycler User Guide...
Page 26: Menu Overview
After you log in to the system, the Main Menu screen opens. This screen is the Screen starting point for operating the Veriti Thermal Cycler. The date, current time, and current user are shown at the bottom of the screen.
Page 27 If your system does not require login, the Manage Users button is not shown. For instruments that require login, only Administrators have access to this menu. Figure 1-6 The Admin Menu screen, available only to users with administrative privileges Veriti™ Thermal Cycler User Guide...
Page 28: About Run Methods
A run method is a set of instructions that specifies how the instrument should heat Method? and cool your samples. Run methods are stored in the instrument firmware. Predefined The Veriti Thermal Cycler firmware has ten predefined run methods Methods ® • AmpliCycle Seq Run Method ®...
Page 29 • View steps with ramp rates – Touch Options, then Ramp Rate. The fields showing times update to show the corresponding ramp rates. 3. When you are done, touch to return to the Browse Run Methods screen. Veriti™ Thermal Cycler User Guide...
Page 30 Start a run In the list of run methods, touch a run method to select it, then touch Start Run (see “Performing a Run” on page 3-18). 1-10 Veriti™ Thermal Cycler User Guide...
Page 31 If you are copying one method – touch Name, then rename the method, and/or touch (Folder) to save the run method to a different folder. If you are copying more than one method – touch (Folder) to save the run methods to a different folder. Veriti™ Thermal Cycler User Guide 1-11...
Page 32 Designating a The run method that is designated as the default method is used as the starting point Run Method as for all new methods. The Veriti Thermal Cycler uses the General PCR run method the Default (shown on page C-5) as the default, unless you change to another method.
Page 33 1. Insert the USB drive into the USB port on the front of the instrument. USB Port 2. Log in as a user with Administrator access. 3. In the Main Menu screen, touch Settings Menu, then Admin Menu. Veriti™ Thermal Cycler User Guide 1-13...
Page 34 2. Open the About the Instrument screen in one of the following ways: • Touch Log Off to return to the Login Options screen, then touch About the Instrument. • In the Main Menu screen, touch Settings Menu, then About the Instrument. 1-14 Veriti™ Thermal Cycler User Guide...
Page 35: About Folders
In addition, you may create up to nine custom folders. In a custom folder, run methods can be accessed, edited, run, and deleted only by the user who created them. In the Manage Folders screen, custom folders have a yellow button. Veriti™ Thermal Cycler User Guide 1-15...
Page 36 4. Enter up to 12 characters for the folder’s new name, then touch Done to return to the Manage Folders screen. 5. Repeat steps 3 to rename any other folders. 6. When you are done making changes to folders, touch (Close) to return to the Settings Menu screen. 1-16 Veriti™ Thermal Cycler User Guide...
Page 37: Chapter 2 Getting Started
Thermal Cycler ........2-7 Veriti™ Thermal Cycler User Guide...
Page 38: Before You Start
‡ At the rear of the instrument to ensure adequate airflow and cooling. § With the heated cover open # Above the instrument to provide adequate space for opening the heated cover, as shown below. 1 square. = 10 cm (3.94 in Veriti™ Thermal Cycler User Guide...
Page 39: Environmental Requirements
Pollution The Veriti Thermal Cycler has a Pollution Degree rating of 2. It may be installed in an environment that has nonconductive pollutants only, such as dust particles or wood chips.
Page 40: Setting Up The Veriti System
System, then inspect the instrument for shipping damage. ™ If the Veriti System is damaged, note the location and IMPORTANT! appearance of the damage, then contact Applied Biosystems Technical Support or your service representative (see “How to Obtain Support” page viii). ™...
Page 41: Connecting To A Network Printer
2-8). Connecting Together Multiple Instruments You Will Need Connecting your Veriti Thermal Cycler to other Veriti Thermal Cyclers allows you to use one instrument to control others. You can connect together up to 12 instruments. To connect together multiple Veriti Thermal Cyclers, you need: •...
Page 42: Powering On
2-7. Powering On 1. Verify that the heated cover is closed. 2. Press the power button on the back left side of the instrument: The instrument powers on and the Main Menu screen is displayed: Veriti™ Thermal Cycler User Guide...
Page 43: Setting Up The Veriti Thermal Cycler
1. In the Main Menu screen, touch Settings Menu. 2. In the Settings Menu screen, touch Admin Menu. 3. In the Admin Menu screen, touch Set IP Address. 4. In the IP Settings screen, set the IP address: Veriti™ Thermal Cycler User Guide...
Page 44 The date and time appear in the run report and at the bottom of many of the screens and Time on the instrument. 1. In the Main Menu screen, touch Settings Menu. 2. In the Settings Menu screen, touch Admin Menu. 3. In the Admin Menu screen, touch Set Date & Time. Veriti™ Thermal Cycler User Guide...
Page 45: Setting Up Instrument Security And User Accounts
6. Touch Done, then OK to save your changes and return to the Admin Menu screen. Setting Up Instrument Security and User Accounts By default, the Veriti Thermal Cycler has all security settings disabled, allowing anyone to use the instrument and/or change instrument settings. To change the security settings for the instrument: 1.
Page 46: Adding Users
1. In the opening screen, touch On, then Log In. 2. In the Log In screen, touch: a. The User field, then enter admin. b. The Password field, then enter password. c. Log In. 3. In the Main Menu screen, touch Settings Menu. 2-10 Veriti™ Thermal Cycler User Guide...
Page 47 The Manage Users screen is displayed: 5. Touch Add User. The Create New User Account screen is displayed: 6. Enter information for the new user. The required fields are User Name, Password, Re-enter Password, and Permission Level. Veriti™ Thermal Cycler User Guide 2-11...
Page 48 3. In the Settings Menu screen, touch Admin Menu. 4. In the Admin Menu screen, touch Manage Users. The Manage Users screen is displayed: 5. In the list, touch the user in the list, then touch Edit User. 2-12 Veriti™ Thermal Cycler User Guide...
Page 49 1. Log in as a user with Administrator access. 2. In the Main Menu screen, touch Settings Menu. 3. In the Settings Menu screen, touch Admin Menu. 4. In the Admin Menu screen, touch Manage Users. The Manage Accounts screen is displayed: Veriti™ Thermal Cycler User Guide 2-13...
Page 50: Setting Other Options
Setting Other Options Table 2 lists additional options that can be adjusted or activated. These settings apply to all users. Table 2 Additional options (and their default values) for the Veriti Thermal Cycler Option Default Value Page Cover: 105 °C...
Page 51 “Creating a Shortcut to a Run Method” on page 1-13). 1. In the Main Menu screen, touch: • Any button labeled Shortcut X (where X is a number). • Settings Menu, then Configure Shortcuts. Veriti™ Thermal Cycler User Guide 2-15...
Page 52 To delete a shortcut, follow the steps above, but uncheck the function to remove it from the Main Menu. Any run methods for which there is a shortcut are shown at the bottom of the list and can also be deleted. 2-16 Veriti™ Thermal Cycler User Guide...
Page 53 Removing the Samples from the Instrument ......3-25 Veriti™ Thermal Cycler User Guide...
Page 54: Chapter 3 Performing A Run
™ Disposables for 96-Well Veriti Thermal Cyclers 96-Well Tube The tray or the tray/retainer is required for the operation of the Veriti IMPORTANT! Thermal Cycler. Configurations You can use three different types of tube configurations to hold your samples, as...
Page 55 Tray/Retainer Set for Retainer ™ without Cap (0.2-mL) Veriti Systems ™ MicroAmp 8-Tube Strip (0.2-mL) ® MicroAmp Reaction Tube without Cap (0.2-mL) ™ MicroAmp 96-Well Tray ™ for Veriti Systems ™ MicroAmp Splash Free 96-Well Base Veriti™ Thermal Cycler User Guide...
Page 56: Disposables For The 384-Well Veriti Thermal Cycler
Chapter 3 Performing a Run ™ Disposables for the 384-Well Veriti Thermal Cycler There is one plate configuration for the Veriti 384-Well Thermal Cycler, shown in Figure 3-2, below. This uses the MicroAmp™ Optical 384-Well Reaction Plate. ™ ™ Figure 3-2 MicroAmp...
Page 57: Loading Samples Into The Instrument
See the appropriate section for your instrument: ™ • “Loading Samples in Veriti 96-Well Thermal Cyclers” on page 3-5 ™ • “Loading Samples in the Veriti 384-Well Thermal Cycler” on page 3-6 ™ • “Loading Samples in the Veriti 60-Well Thermal Cycler” on page 3-7 ™...
Page 58: Loading Samples In The Veriti 384-Well Thermal Cycler
Do not place the base in the sample block(s). IMPORTANT! 3. Close the heated cover. ™ Loading Samples in the Veriti 384-Well Thermal Cycler This section describes how to load samples in 384-well reaction plates. 1. Pipette the samples into the sample wells.
Page 59: Loading Samples In The Veriti 60-Well Thermal Cycler
Chapter 3 Performing a Run ™ Loading Samples in the Veriti 60-Well Thermal Cycler There is no need to use mineral oil or glycerine in the sample wells or as a vapor ® barrier over the PCR reaction mixture in the tubes. The GeneAmp...
Page 60: Creating A New Run Method
“Adding a Pause” on page 3-15). • Delete a step or stage (see “Deleting a Stage or Step” on page 3-15). • For Veriti 96-Well Thermal Cyclers, you can add a step to program ™ ™ different temperatures on the VeriFlex Blocks (see “Creating a VeriFlex...
Page 61 You can change this temperature when you perform a run with this run method. 9. Touch Notes, then enter any notes for the method. 10. Touch Save & Exit when you are done. The Browse Run Methods screen is displayed. Veriti™ Thermal Cycler User Guide...
Page 62: Editing Details
3-15) • Creating a step to program different temperatures on the VeriFlex Blocks (page 3-16) (Available only on Veriti 96-Well Thermal Cyclers.) Adding a Stage The two types of run method stages are: • Cycling – The steps in a cycling stage are repeated a specified number of times.
Page 63 1. In the Edit Run Method screen, touch anywhere in the column for a step to select it. When the step is selected, the column turns red: 2. Touch Add. A copy of the selected step is added to the run method: Veriti™ Thermal Cycler User Guide 3-11...
Page 64 3. Touch the ramp field, then enter the desired ramp rate. Ramp rates from 0.1 to 100% are valid. 4. Touch Done to close the editor. The ramp rate symbol appears next to any step with a ramp rate less than 100%. 3-12 Veriti™ Thermal Cycler User Guide...
Page 65 AutoDelta steps are allowed only in cycling stages. Note: To create an AutoDelta step: 1. Touch a step, then Options. The options menu appears: Veriti™ Thermal Cycler User Guide 3-13...
Page 66 5. Touch OK to save the step and return to the screen that displays the run method. The AutoDelta symbol appears on the step with the AutoDelta step. In this example, Step 2 in Stage 2 is an AutoDelta step: 3-14 Veriti™ Thermal Cycler User Guide...
Page 67 Deleting a Stage 1. In the Edit Run Method screen, touch the stage or step to select it. or Step 2. Touch Delete, then OK. The stage or step is deleted from the run method. Veriti™ Thermal Cycler User Guide 3-15...
Page 68 Chapter 3 Performing a Run Creating a This function is only available for the 96-well versions of the Veriti Thermal Note: ™ Cycler. VeriFlex Step ™ The Veriti 96-Well Thermal Cyclers have six VeriFlex blocks. You can specify ™ ™...
Page 69 • Touch Time, enter the duration (in minutes and seconds), then touch Done. • Touch Ramp Rate, enter the ramp rate (as a percentage value), then touch Done. The VeriFlex Step appears in the Edit Run Method screen, with the text “Zones” in the temperature field. Veriti™ Thermal Cycler User Guide 3-17...
Page 70: Performing A Run
Reaction volumes are specific to the type of instrument: • Veriti 96-Well Fast Thermal Cycler: 5 to 40 µL • Veriti 96-Well Thermal Cycler: 10 to 100 µL • Veriti 384-Well Thermal Cycler: 5 to 30 µL •...
Page 71 After the run begins, you must interact with any remote instruments directly if you want to make changes to the run method, pause, or stop the run. When the runs are complete, the Run Reports are displayed on each instrument. Veriti™ Thermal Cycler User Guide 3-19...
Page 72 10. While the cover heats, the Cover Heating dialog box appears in the Run Monitor screen: If you want to: • Start the run immediately, before the cover reaches the specified temperature – Touch (Skip). • Stop the run – Touch (Stop). 3-20 Veriti™ Thermal Cycler User Guide...
Page 73: Monitoring A Run
Indicates that the heated cover has achieved a temperature sufficient to burn skin. ™ Stage Indicator Indicates the stage that the Veriti System is performing and the current cycle in that stage. Step Temperature Indicates the temperature for the step.
Page 74 “Changing the Number of Cycles” on page 3-22). Go to the Main Menu screen Touch (Home). From the Main Menu Screen, you can access most of the Veriti Thermal Cycler functions while the run continues. View the Status Report Touch (Status).
Page 75 • Resume the run immediately – Touch Resume Run (to the left of the dialog box). Stopping a Run In the Run Monitor screen, touch Stop, then OK. The run stops and the Run Report opens. Veriti™ Thermal Cycler User Guide 3-23...
Page 76 20 cycles. After the power returns, the run starts at step 1 of the tenth cycle and continues for 11 more cycles. If the time between the initial power failure and the time the power returns is Note: more than 18 hours, the run is not continued. 3-24 Veriti™ Thermal Cycler User Guide...
Page 77: Viewing And Printing The Run Report
Grasp the sample tray/retainer assembly or reaction plate firmly with both hands and gently rock it back and forth until the tubes (or plate) are free. Sample caps may pop off if the cover is opened when the sample block(s) temperature is above 27 °C. Veriti™ Thermal Cycler User Guide 3-25...
Page 78 Chapter 3 Performing a Run 3-26 Veriti™ Thermal Cycler User Guide...
Page 79: Chapter 4 Troubleshooting
Returning an Instrument for Service ........4-3 Veriti™ Thermal Cycler User Guide...
Page 80 Chapter 4 Troubleshooting Troubleshooting ™ Use the following table to help troubleshoot problems with your Veriti Thermal Cycler. Problem Possible Causes Check and/or Remedy Cooling rate too slow • Peltier assembly failure • Run the Cycle Performance Test. Temperature does not match •...
Page 81: Returning An Instrument For Service
Repairs for instruments without the Certificate of Instrument Note: Decontamination are delayed. 6. Affix the provided postage to the box, then ship the instrument to the designated facility. The repair process requires 2 to 3 weeks. Veriti™ Thermal Cycler User Guide...
Page 82 Chapter 4 Troubleshooting Veriti™ Thermal Cycler User Guide...
Page 83 Tests Using the Temperature Verification Kit ......5-11 Veriti™ Thermal Cycler User Guide...
Page 84: Chapter 5 Utilities
GeneAmp® PCR System 9700 thermal cycler. The tool is designed to create a method for the Veriti Thermal Cycler with ramp rates that simulate those on the 9700 thermal cycler. Although the ramp rates may be...
Page 85 “9700-Max-Mode-” or “9600-Mode-”, depending on the format selected in step Also enter the reaction volume, the temperature for the heated cover (if any), and any notes for this method. When you finish, touch Save & Exit. The Tools Menu is displayed. Veriti™ Thermal Cycler User Guide...
Page 86 This is indicated by “9600” or “9700M” in the Time field, when you choose to view ramp rates rather than time (see Figure 5-1). Figure 5-1 Converted run method, showing “9700M” as the ramp rate Veriti™ Thermal Cycler User Guide...
Page 87: Upgrading System Firmware
Chapter 5 Utilities Upgrading System Firmware About the If Applied Biosystems provides a new version of the firmware for the Veriti Thermal Upgrade Cycler, you may be required to load the new firmware on the instrument. You will need a USB drive and, if your instrument requires login, Administrator access to upgrade the firmware.
Page 88 6. In the Admin Menu screen, touch Upgrade Firmware. The Firmware Upgrade screen opens: ™ 7. Touch Source File to display a list of the files on the USB drive. Veriti system firmware files end with “.update”. 8. Touch the name of the file with the new firmware, then touch Select.
Page 89: Calculating Melting Temperatures
Touch Salt Concentration, then enter the salt concentration in mM. b. Touch Primer Concentration, then enter the DNA concentration in µM. c. Enter each primer sequence. 4. Touch Calculate. The melting temperature for each primer is displayed: Veriti™ Thermal Cycler User Guide...
Page 90: Viewing Instrument Statistics
1. Log in as a user with Administrator access. 2. In the Main Menu screen, touch Settings Menu. 3. In the Settings Menu screen, touch Admin Menu. Veriti™ Thermal Cycler User Guide...
Page 91 7. Remove the USB drive, if present, then toggle the switch on the back of the instrument to power off. 8. Toggle the switch again to power on the instrument and restart with the factory default settings. Veriti™ Thermal Cycler User Guide...
Page 92: Running The Cycle Performance Test
The results are saved to the file CyclePEvents.txt. 6. If the test fails, repeat the procedure. Ensure that the heated cover is closed. 7. If the test fails again, contact Applied Biosystems Technical Support. 5-10 Veriti™ Thermal Cycler User Guide...
Page 93: Tests Using The Temperature Verification Kit
• Digital thermometer – Model 4690 Digital thermometer with 9 V battery • Light mineral oil • Cotton swabs You need the appropriate model of the Temperature Verification Kit for your Veriti Thermal Cycler, as shown below. Part Veriti Thermal Cycler Model...
Page 94: Running The Temperature Verification
Running the Temperature Verification Use this test to verify that the average temperature for the sample block(s) is within ±0.25 °C of the set point temperature. Required The appropriate Temperature Verification Kit for your Veriti Thermal Cycler model Materials (see table above). Procedure This test takes 25 to 30 minutes to complete, but you can cancel before it is complete.
Page 95: Running The Temperature Nonuniformity Test
Running the Temperature Nonuniformity Test Use this test to verify that the sample well temperatures are within the threshold temperature range. Required The appropriate Temperature Verification Kit for your Veriti Thermal Cycler model Materials (see table above). Procedure This test takes 10 to 15 minutes to complete, but you can cancel before it is complete.
Page 96 Chapter 5 Utilities 5-14 Veriti™ Thermal Cycler User Guide...
Page 97: Maintenance
Parts List ............6-6 Veriti™ Thermal Cycler User Guide...
Page 98: Scheduled Maintenance
5-13). Cleaning the Instrument Preparation Before cleaning the instrument: ™ 1. Power off the Veriti Thermal Cycler, then remove the power cord. 2. Allow the instrument to cool until the heated cover and sample block(s) reach room temperature. Cleaning the Clean the touchscreen with any commercially available LCD cleaning product.
Page 99 • After treating with bleach solution, rinse the sample block(s) thoroughly using deionized water. Removing residual bleach from the surfaces of the Veriti™ instrument using Note: water minimizes the long-term effects of bleach treatments. If the sample block(s) become contaminated with radioactivity, use a commercially available decontaminant to remove the contamination.
Page 100: Replacing Fuses
To avoid electrical shock, disconnect the power supply to the instrument, unplug the power cord, and wait at least 1 minute before working on the instrument. Veriti™ Thermal Cycler User Guide...
Page 101 5. Place the fuse compartment back into the power entry module (there is only one orientation) and close the door. 6. Press the door until it locks in place. 7. Connect the instrument power cord. Veriti™ Thermal Cycler User Guide...
Page 102: Parts List
Panel, Left 4374176 Panel, Rear (Heated Cover) 4374280 Panel, Right 4374175 Probe, Temperature Verification Kit (0.1-mL) 4375938 Probe, Temperature Verification Kit (0.2-mL) 4377670 Probe, Temperature Verification Kit (0.02-mL) 4308757 Probe, Temperature Verification Kit (0.5-mL) 4309925 Veriti™ Thermal Cycler User Guide...
Page 103: Appendix A Instrument Specifications
Printer Specifications ..........A-3 Veriti™ Thermal Cycler User Guide...
Page 104: System Specifications
USB v1.0 port for a connection to an external USB drive RS232 serial communication port for connecting the probe for the Temperature Verification Kit ™ Figure A-1 Connections for power and communications on the Veriti Thermal Cycler Veriti™ Thermal Cycler User Guide...
Page 105: Printer Specifications
0.02 mL Veriti 60-Well Thermal Cycler (PN 4384638) 0.5 mL Printer Specifications The Veriti Thermal Cycler requires a printer that: • Has an Ethernet port • Supports the LPD/LPR protocol (often called a “TCP/IP printer”) Veriti™ Thermal Cycler User Guide...
Page 106 Chapter A Instrument Specifications Veriti™ Thermal Cycler User Guide...
Page 107: Appendix B Disposables
Other Supplies for Sample Handling........B-4 Veriti™ Thermal Cycler User Guide...
Page 108 Chapter B Disposables Disposables ™ Table B-1 Disposables for the Veriti Thermal Cycler 96-Well 96-Well 384-Well 60-Well Part Part Description (0.1-mL) (0.2-mL) (0.02-mL) (0.5-mL) Number 96-Well Plates MicroAmp™ Fast 96-Well Reaction Plate (0.1 mL) 4346907 MicroAmp™ Fast Optical 96-Well Reaction Plate 4346906 with Barcode (0.1 mL)
Page 109 Chapter B Disposables ™ Table B-1 Disposables for the Veriti Thermal Cycler (continued) 96-Well 96-Well 384-Well 60-Well Part Part Description (0.1-mL) (0.2-mL) (0.02-mL) (0.5-mL) Number MicroAmp™ 8-Cap Strip N8010535 N8011535 MicroAmp™ 8-Cap Strip, Assorted Colors N8010835 MicroAmp™ Optical 8-Cap Strip...
Page 110: Other Supplies For Sample Handling
Chapter B Disposables Other Supplies for Sample Handling ™ The following parts can simplify working with your samples and the Veriti Thermal Cycler. Product Part Number MicroAmp™ 96-Well Base N8010531 MicroAmp™ Splash Free 96-Well Base 4312063 ® GeneAmp Thermal Isolation Frame 4308927 MicroAmp™...
Page 111: Appendix C Predefined Run Methods
Predefined Run Methods..........C-2 Veriti™ Thermal Cycler User Guide...
Page 112: Amplicycle Seq Run Method
Thermal Cycler and are stored in the Shared folder. These run methods are based on recommended protocols from Applied Biosystems and should be considered as starting points for the different applications. Your results may vary between instrument models, so some optimization may be needed.
Page 113: Amplitaq Gold ® Fast Pcr Run Method
DNA sequencing reactions and is only available ™ on Veriti 96-Well Thermal Cyclers. By default, the annealing temperature is set at 62 °C. Replace this temperature Note: with the melting temperature of the primer (see “Calculating Melting Temperatures” on page 5-7). Veriti™ Thermal Cycler User Guide...
Page 114: Bigdye ® Kit Fast Run Method
(Sanger) terminator sequencing, with ABI P BigDye Terminator Cycle RISM Sequencing Ready Reaction Kits. ® The pre-coded conditions in this run method are optimized for AmpliTaq ® Polymerase, FS and the cycle sequencing reagents supplied with the BigDye Terminator kits. Veriti™ Thermal Cycler User Guide...
Page 115: Fast Pcr Run Method
Fast PCR Master Mix available from www.appliedbioystems.com. General PCR Run Method Use this run method for standard PCR use. This method is set as the default run method for the Veriti Thermal Cycler. Veriti™ Thermal Cycler User Guide...
Page 116: Lms2 Run Method
10 additional cycles, allowing for slow activation of the enzyme during cycling. This provides a simple method where polymerase activity increases more slowly as product accumulates, improving specificity. Veriti™ Thermal Cycler User Guide...
Page 117: Touchdown Pcr Run Method
You can use XL PCR to amplify the control target (a 20.8-kb product from Lambda DNA) supplied in the kit. Veriti™ Thermal Cycler User Guide...
Page 118 Chapter C Predefined Run Methods Veriti™ Thermal Cycler User Guide...
Page 119: Index
Getting Started shortcuts 2-16 Maintenance steps or stages 3-15 Performing a Run users 2-13 System Overview Display Method Time button 3-22 Troubleshooting Disposables appendix Utilities documentation, related viii chemical safety chemical waste safety xvii Veriti™ Thermal Cycler User Guide Index-1...
Page 120 IMPORTANT, description power failure during a run 3-24 Information Development department, contacting viii Predefined Run Methods appendix installation category xvii printing instrument operation, safety run methods 1-12 instrument properties run report 3-25 IP address Index-2 Veriti™ Thermal Cycler User Guide...
Page 121 3-15 pausing before 3-15 skipping during a run 3-22 safety VeriFlex 3-16 before operating the instrument STOP button 3-22 chemical symbols, safety xiii chemical waste xvii System Overview chapter conventions electrical xvii guidelines xvii Veriti™ Thermal Cycler User Guide Index-3...
Page 122 TVK See temperature verification kit 5-11 upgrading firmware USB port user attention words, described users access levels 2-10 adding 2-10 changing 2-12 default administrator user name and password 2-10 deleting 2-13 Manage Users button missing Utilities chapter Index-4 Veriti™ Thermal Cycler User Guide...
Page 124 Worldwide Sales and Support Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 150 countries on six continents. For sales office locations and technical support, please call our local office or refer to our Web site at www.appliedbiosystems.com.

Applied Biosystems Veriti User Manual

Chapter 1 System Overview

Chapter 2 Getting Started

Chapter 3 Performing a Run

Chapter 4 Troubleshooting

Chapter 5 Utilities

Appendix A Instrument Specifications

Appendix B Disposables

Appendix C Predefined Run Methods

Quick Links

Need help?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for Applied Biosystems Veriti

Table of Contents