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Summary of Contents for Nexcope NE620T
- Page 1 NE620T UPRIGHT BIOLOGICAL MICROSCOPE FOR PROFESSIONAL APPLICATIONS INSTRUCTION MANUAL NE620T U P R I G H T R E S E A R C H M I C R O S C O P E...
- Page 2 WE CONGRATULATE YOU on your purchase of the professional research microscope NEXCOPE NE620T. It has been carefully manufactured with materials of lasting value, and careful attention has been paid to function and reliability. This microscope is designed for a wide range of applications. It fea- tures a very high contrast, which ensures an outstanding image quality and resolution.
- Page 3 (código QR) para ver las versiones disponibles. Desidera ricevere informazioni esaustive su questo prodotto in una lingua specifica? Venga a visitare il nostro sito Web al seguente link (codice QR Code) per conoscere le versioni disponibili. www.bresser.de/download/nexcope GARANTIE · WARRANTY · GARANTÍA · GARANZIA www.bresser.de/warranty_terms We recommend that you study this manual thoroughly before operating the microscope for the first time.
- Page 4 SERVICE You can obtain additional information from your customer service: DE AT CH BE NL BE Bei Fragen zum Produkt und eventuellen Reklamationen Als u met betrekking tot het product vragen of nehmen Sie bitte zunächst mit dem Service-Center Kontakt eventuele klachten heeft kunt u contact opnemen auf, vorzugsweise per E-Mail.
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Page 5: Table Of Contents
Disposal ........................Introduction ......................2.1. What you can expect… ..................Design of NE620T ....................3.1. Product image NE620T - biological microscope ..........NE620T front/side view ..................NE620T back/side view ..................3.2. Assembly of the NE620T microscope ..............Start-up and Operation ................... -
Page 6: Important Notes
IMPORTANT NOTES IMPORTANT NOTES This chapter informs the user about the general instructions for the microscope and the im- portant safety symbols. 1.1. Imprint/validity information Imprint Bresser GmbH Gutenbergstraße 2 46414 Rhede Deutschland http://www.bresser.de For information regarding liability claims or service requests, please refer to chapters "Warranty"... -
Page 7: General Safety Instructions
IMPORTANT NOTES 1.3. General safety instructions Danger of an electric shock! This device contains electronic components that operate via a power source (power supply and/or batteries). In case of any improper use of this device, there is a risk of an electric shock. - Page 8 For this device only use accessories and spare parts that comply with the technical information. • Always use the power cord supplied by Nexcope. If an unsuitable power cord is used, Nexcope can no longer guarantee the electrical functionality and safety of the micro- scope.
- Page 9 IMPORTANT NOTES LED fluorescent attachment - CAUTION: LED radiation LED class 3B, 3W LED, FL-B (blue excitation: 460-495 nm), FL-G (green excitation: 510-550 nm) • Do not expose to the beam. Avoid irradiation of the skin! The microscope is equipped with a green and blue LED illumination. Neve look direct- ly into the illumination or directly at the illuminated specimen.
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Page 10: Safety Symbols Used In The Manual
IMPORTANT NOTES 1.4. Safety symbols used in the manual Danger symbols Warning of a danger point This symbol indicates information that must be read and observed. Non-observance can lead to: o Risk of injury o Malfunctions or equipment damage Warning of dangerous electrical voltage This symbol precedes information that must be read and observed. -
Page 11: Check Scope Of Delivery And Handling
IMPORTANT NOTES 1.6. Check scope of delivery • Open the packaging with care. Avoid fingerprints and sweat on the camera lens. Pre- vent the camera and some accessories from falling and being damaged. • Remove all components from the packaging and check for completeness according to the delivery note. -
Page 12: Cleaning/Maintenance/Transport
IMPORTANT NOTES 1.8. Cleaning/maintenance/transport Disconnect the device from the power supply by pulling the power plug when it is not used or in case of longer interruption of operation and before starting any work on maintenance and cleaning. Disassemble optical elements (e.g.objective, eyepiece, etc.) before cleaning. Be sure that the instrument is dry before using. -
Page 13: Protection And Storage
IMPORTANT NOTES Immersion oil • When using immersion oil, it is essential to read the relevant safety data sheet. Immersion oil irritates the skin. Therefore avoid contact with skin, eyes and clothing. • In case of skin contact: wash off with plenty of water and soap. •... -
Page 14: Disposal
IMPORTANT NOTES 1.10. Disposal Dispose of the packaging materials properly, according to their type, such as paper or card- board. Contact your local waste-disposal service or environmental authority for information on the proper disposal. Do not dispose of electronic devices in the household garbage! As per Directive 2002/96/EC of the European Parliament on waste electrical and electronic equipment and its adaptation into German law, used electronic devices must be collected separately and recycled in an environmentally friendly manner. -
Page 15: Introduction
NE620T uses an ergonomic design, high eye-point, low-hand focus mechanism, low-hand stage and other ergonomic designs to ensure the user can perform mi- croscope operation in the most comfortable situation. As a continuously upgradeable microscope, the NE620T can be extended by a variety of observation possibilities. -
Page 16: Design Of Ne620T
Illustration 001: Product image of NE620T front/side view. AUTOMATIC DESIGN BASIC DESIGN 9. Liquid Crystal Display (LCD) 1. Main body 12. Condenser with aperture diaphragm 9a. Selected objective 2. Condenser up-down knob 13. -
Page 17: Ne620T Back/Side View
Illustration 002: Product image of NE620T back/side view. BASIC DESIGN 21. Viewing head holding screw 31. Type plate with important information 22. Condenser centering screw (on both sides) 32. USB interface 23. Condenser holding screw 33. -
Page 18: Assembly Of The Ne620T Microscope
DESIGN OF NE620T 3.2. Assembly of the NE620T microscope The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly. IF NOT PRE-INSTALLED Illustration 003: Assembly of NE620T. When assembling the microscope, make sure that all parts are free of dust and dirt and avoid scratch- ing any parts or touching glass surfaces. - Page 19 DESIGN OF NE620T 3.2.1. Detailed assembly procedure Attaching the power cord and startup of the device Illustration 004: Connecting the power cord and commissioning. Move On/Off switch (V) to position I to turn Connect the cold-device plug (I) to the ap-...
- Page 20 DESIGN OF NE620T Installing objectives Turn the encoded quintuple objective nose- Illustration A/B: Installing objectives: Preparatory measures. piece (III) by hand until the LCD shows the Use the coarse focusing knob (I) to turn the programmed position for the 4x objective cross stage to the lowest position.
- Page 21 DESIGN OF NE620T Attaching the trinocular viewing head Illustration 005: Attaching the trinocular viewing head. If present pull off the black protective cap (I) from the tube lens (at the bottom of the trinocular head). Make sure not to touch any optical lenses.
- Page 22 DESIGN OF NE620T Using the C-mount camera adapters 0.5x C-mount Illustration 006: Attaching the C-mount camera adapters. Using a C-mount camera If you do not want to use a camera, please use the appropriate protective cap (V) of the If not already pre-installed, place the C-mount camera adapter to protect the interface from adapter (0.5x or 1x - depending on the cam-...
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Page 23: Start-Up And Operation
START-UP AND OPERATION 4. START-UP AND OPERATION 4.1. Setting up power supply Illustration 007: Setting up power supply. Connect the cold-device plug (1) to a suitable Switch on the device by setting the On/Off mains in (2) on the back of the microscope. switch (5) to position I on the left side. -
Page 24: Placing The Specimen
START-UP AND OPERATION 4.2. Placing the specimen Cross stage for acquisition, positioning and fixation of the specimens with clamp holder. Illustration 008: Placing the specimen on the cross stage. Turn the coarse focusing knob (1) in the di- The specimen slide (3) can easily be placed rection of the blue arrow to lower the cross by moving it into the specimen clamp holder stage a little (2). -
Page 25: Observationtubus
START-UP AND OPERATION 4.3. Observationtubus 1. Adjusting the interpupillary distance (Distance between the eyepieces) Look through the eyepieces (1) and adjust the interpupillary distance (2) so that the left and right fields of view are completely aligned. The device can be adjusted to the interpu- pillary distance of the respective observer between 47 mm and 78 mm. -
Page 26: Focusing The Specimen
START-UP AND OPERATION 4.4. Focusing the specimen 4.4.1. Focusing controls Right-hand side Left-hand side Illustration 012: Focusing controls. Fine focusing knobs are located comfortably Turn the coarse focusing knob (1) to raise or on each side of the microscope. lower the stage: The right fine focusing knob (2b) has six small recesses to make fine adjustment even Stage moves up and vice versa... - Page 27 The Liquid Crystal Display on the front of the microscope can display the different working modes of the microscope, including magnification, light intensity, SLEEP mode, and so on. NE620T can store and automatically adjust the light intensity for each objective. This im- proves work efficiency and reduces visual fatigue.
- Page 28 SLEEP PRESS 1x Illustration 016: D: LCD of NE620T: STANDBY/SLEEP mode and START/WORKING mode. In STANDBY/SLEEP mode, the light is switched off to save energy and lamp life. Press once or turn the brightness control knob (4) - both directions possible - to change from STANDBY/SLEEP mode to START/WORKING mode.
- Page 29 START-UP AND OPERATION 4.5. Bright field observation according to Koehler 4.5.1. Centering the condenser Illustration 017: Centering the condenser. PRESS and TURN the brightness control knob (1) to turn transmitted light ON Turn the condenser up-down knob (2) in the direction of the blue arrow to raise the condens- er to its upper limit.
- Page 30 START-UP AND OPERATION 4.5.2. Aperture diaphragm (condenser diaphragm) 70-80% 30-20% Illustration 018: Adjustment of aperture diaphragm. Two possible ways to adjust the aperture diaphragm (condenser diaphragm): First possibility • Turn the aperture diaphragm ring (1) to the same number as the objective magnification (2).
- Page 31 NE620T achieves the wide field of 22 mm view with 10x eyepieces for a more compre- hensive observation content and faster sample observation. The eyepiece adopts a flat field distortion-free design to prevent the edge of the field from being imaginary and stray light.
- Page 32 START-UP AND OPERATION • Immersion objectives 100x/Oil Illustration 020: The 100x oil-immersion objective. The oil immersion can only be done with a suitable 100x objective! The 100x oil objective is included in the scope of delivery. Cedar oil is included in the delivery. We recommend oil with a refractive index (RI) nD=1.515 according to ISO 8036.
- Page 33 START-UP AND OPERATION • Water-immersion objective Water (1) is used instead of cedar oil or immersion oil for 100x water-immersion objective (2). Excellent image, easy to operate better and easier cleaning and therefore better environmental performance. Water-immersion 100x objective is not included in the delivery but is available as accessory.
- Page 34 START-UP AND OPERATION 4.7. Exchange of the fuse Unplug the power plug before changing the fuse! If fuses should fail, the cause must first be determined and any techni- cal faults taken care of. Pull the cold-device plug (1) from the mains connection (2) at the back of the microscope.
- Page 35 START-UP AND OPERATION 4.8. LED lamp replacement Unplug the power plug before changing the LED lamp! In general, the LED lamp is very du- rable. If it is damaged, please contact the customer service to get a replace- ment LED. Have suitable tools ready for the change! Place the microscope on the microscope...
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Page 36: Various Observation Methods
VARIOUS OBSERVATION METHODS 5. VARIOUS OBSERVATION METHODS As a continually upgradable microscope, NE620T can be extended on basic model to show a variety of observation capabilities. The accessory sets are not included in the scope of delivery and can be ordered separately. - Page 37 VARIOUS OBSERVATION METHODS 5.1.2. Components for dark field observation Included in scope of delivery: • Infinity-corrected plan objectives (4x, 10x, 40x, 100x) Not included in the scope of delivery but available separately: • Bright field/dark field slider (BF/DF slider) 5.1.3. Settings for dark field observation Illustration 025 A-B: Settings for dark field observation: Removing the placeholder on condenser.
- Page 38 VARIOUS OBSERVATION METHODS Illustration 026 C-D: Settings for dark field observation: Inserting the BF/DF slider. With the BF/DF slider an observation For optimal dark field observation: in bright field and dark field is pos- Add a drop of cedar oil/immersion oil (8) sible: between the condenser and the specimen so Loosen the right-hand screw (5) of the BF/...
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Page 39: Phase Contrast Observation
VARIOUS OBSERVATION METHODS Final check and dark field observation 1x drop of cedar oil/immersion oil between condenser and object slide Aperture diaphragm ring set to maximum position 100/ PH/D. Dark field slider mounted Field diaphragm opened to the size of the view ... - Page 40 VARIOUS OBSERVATION METHODS 5.2.2. Components for phase contrast observation Not included in the scope of delivery but available separately: • 4 plan phase contrast objectives: 10x, 20x, 40x, 100x • 2 phase contrast slider: 10/20/40 10/20/40/BF phase contrast slider match- 100/BF phase contrast slider matches with es with the 10x/20x/40x phase contrast the 100x phase contrast objective.
- Page 41 VARIOUS OBSERVATION METHODS 5.2.3. Settings for phase contrast observation 10xPh 100x/Oil Illustration 028 A-B: Settings for phase contrast: Inserting the appropri- ate objectives. Now replace the objectives with the phase All settings, including adjustment of contrast objectives (3) on the nosepiece (4). the lighting according to Koehler, were made in bright field.
- Page 42 VARIOUS OBSERVATION METHODS 10/20/40 Illustration 030 E: Settings for phase contrast: Mounting corresponding phase contrast slider. With both sliders, phase contrast and bright Insert the phase contrast slider into the corre- field (BF) observation is possible. sponding hole of condenser (8). Attach the right screw (9) of the slider and screw it tight.
- Page 43 VARIOUS OBSERVATION METHODS ALLEN WRENCH BRIGHT RING DIAPHRAGM DARK PHASE RING Illustration 031 F-G: Settings for phase contrast: Centering the phase rings. Centering of the phase rings Check the centering and overlap of the bright ring diaphragm (in the condenser) with the dark phase ring (in the objective).
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Page 44: Polarization Observation
VARIOUS OBSERVATION METHODS Final check and phase contrast observation CT replaced by original eyepiece Phase contrast objectives mounted Corresponding phase contrast slider mounted and phase rings centered Aperture diaphragm ring set to maximum position 100/ PH/D ... - Page 45 VARIOUS OBSERVATION METHODS 5.3.2. Components for polarization observation Included in scope of delivery: • Infinity-corrected plan objectives (4x, 10x, 40x, 100x) Not included in the scope of delivery but available separately: POLARIZER ANALYZER With the help of the analyzer ring you can turn the analyzer from 0° to 90°. In orthogonal polarization observa- tion, it is necessary to turn the ana- lyzer to make the vibration directions...
- Page 46 VARIOUS OBSERVATION METHODS 5.3.3. Settings for polarization observation Illustration 033: A-B: Setting for polarization: Assembly of the polarizer and analyzer. All settings, including adjustment of Remove the rubber placeholder (3) on the the lighting according to Koehler, objective nosepiece (4) and insert the ana- were made in bright field.
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Page 47: Fluorescent Observation
Fluorescence in general produces bright signals against a dark background. Source: Nexcope Structures are imaged larger than they are in reality by fluorescence microsco- py, so it is difficult to determine their size. - Page 48 VARIOUS OBSERVATION METHODS 5.4.2. Safety instructions BEFORE USE • The LED-fluorescent attachment is a precise instrument. Open the box carefully, and avoid dropping the accessories to ground and causing damage to them. • Protect the instrument from direct sunlight, high temperature, humidity, dust and strong vibration.
- Page 49 VARIOUS OBSERVATION METHODS 5.4.3. Components for fluorescent observation Components for fluorescent observation are not included in scope of delivery but available separately: Illustration 034: A-B: Components of the LED fluorescent attachment. The LED fluorescent attachment (I) uses 3W As an accessory to the LED fluorescent at- LED as illuminant and consist of two fluores- tachment you will find an orange radiation cent modules (fluorescence filter cubes, each...
- Page 50 VARIOUS OBSERVATION METHODS 5.4.4. Installation of the LED fluorescent attachment Illustration 035: Installation of the LED fluorescent attachment. Step by step… Loosen the viewing head holding screw Remove the black protective cap (III) (I) for the trinocular viewing head and care- from the bottom of the LED fluorescent attach- fully lift the microscope head (II) off the mi- ment (IV) and place the attachment correctly...
- Page 51 VARIOUS OBSERVATION METHODS VIII ROUND PLUG CONNECTION DC 5V Illustration 036: Installation of the LED fluorescent attachment. Slide the orange protective shield (VII) Connect the adapter (VIII) to the round into the two fixtures at the bottom of the LED plug connection of the LED fluorescent at- fluorescent attachment (IV).
- Page 52 VARIOUS OBSERVATION METHODS 5.4.5. Settings for reflected light fluorescent observation PRESS AND TURN Illustration 037: Settings for reflected light fluorescent microscopy. Move On/Off switch (1) to position I to turn on the microscope. BEFORE YOU START fluorescence observation, it is recommended to set the microscope in bright field mode.
- Page 53 VARIOUS OBSERVATION METHODS Final check and reflected light fluorescent observation LED fluorescent attachment safely installed and adapter connected Set the desired fluorescent module and brightness adjusted Orange protective shield mounted Transmitted light switched off Microscope on and brightness adjusted FOCUS ON THE SPECIMEN Illustration 038 B: Settings for fluorescent observation: Final check and observation.
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Page 54: Equipment Overview
EQUIPMENT OVERVIEW 6. EQUIPMENT OVERVIEW 6.1. Main specifications Components Name Specification 620T Optical system Infinite optical system Seidentopf Binocular Head, 30° Inclined, Interpipillary 47-78mm Seidentopf Trinocular Head (50:50) Viewing Head Seidentopf Trinocular Head (0:100/100:0) Digital Viewing Head ... -
Page 55: Objective Parameters
EQUIPMENT OVERVIEW 6.2. Objective parameters Numerical Working Conjugate Type Magnification Aperture Distance Distance (N.A.) (mm) ∞ 4× 0.10 20.6 ∞ 10× 0.25 17.9 Plan Achromatic ∞ 20× 0.40 Objective (NIS45) ∞ 40× 0.65 ∞ 100× (Water) 0.16 ∞ 2× 0.06 ∞... -
Page 56: Trouble Shooting Guide
TROUBLE SHOOTING GUIDE 7. TROUBLE SHOOTING GUIDE Under certain conditions performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take action as needed. If you can- not solve the problem after checking the entire list, please contact Bresser GmbH for assistance. 7.1. -
Page 57: Mechanical System
TROUBLE SHOOTING GUIDE PROBLEM CAUSE SOLUTION One side of image is blurred Condenser is not properly centered Reinstall the condenser and center or inclined the condenser with the centering screws The nosepiece is not properly Engage the nosepiece properly engaged The specimen is not clamped Fix the sample with the specimen clamp... -
Page 58: Electrical System
TROUBLE SHOOTING GUIDE 7.3. Electrical system PROBLEM CAUSE SOLUTION The lamp cannot light when No power supply Check the connection of the power the switch is turned on cord The connector of LED lamp is not Insert it correctly properly inserted into the circuit board The LED lamp is broken Replace the lamp The lamp burns out suddenly... -
Page 59: Warranty
WARRANTY 8. WARRANTY The regular warranty period is 2 years and starts on the day of purchase. For full warranty terms and services, please visit www.bresser.de/warranty_terms. - Page 60 NOTES/COMMENTS 9. NOTES/COMMENTS...
- Page 61 NOTES/COMMENTS...
- Page 62 NOTES/COMMENTS 9. NOTES/COMMENTS...
- Page 63 NOTES/COMMENTS...
- Page 64 Exclusive distribution for Germany and BeNeLux: BRESSER GmbH Nexcope Service Gutenbergstr. 2 DE-46414 Rhede Germany www.bresser.de service.nexcope@bresser.de Manual_NE-620T_en_NEXCOPE_v122021a...
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