Page 1 SEC2020 SPECTROMETER SYSTEM USER’S GUIDE - SOFTWARE - Document Version: 2.9.0 Last Revision: April 2022...
Page 2: Table Of Contents
Transmittance of Sample ....................35 7-4. Saving Transmittance Measurement .................36 Reflectance Measurement ......................37 8-1. Creating a Reflectance Measurement ................37 8-2. Setting Measurement Conditions ..................37 8-3. Reflectance of Sample .......................38 8-4. Saving Reflectance Measurement ..................39 Strip Chart (Recording Specific Wavelength) ................40 https://www.als-japan.com...
Page 3 14-6. Graph Toolbar Icons ......................87 14-7. Spectrum Data Pane ......................95 14-8. Saving Currrent Spectrum ....................98 14-9. Other Tool Bar Icons ......................102 Spectrum Correction Features ....................108 15-1. Electronic Dark Correction ....................108 15-2. Linearity Correction ......................110 Contact Information ......................... 111 https://www.als-japan.com...
Page 4: About Sec Spectra
SEC Spectra User's Guide 1. About SEC Spectra 1. About SEC Spectra SEC Spectra is a software program for the portable spectrometers system “SEC2020 Spectrometer system”. It offers a wide variety of optical measurements including spectrum, absorbance, transmittance, reflectance, Relative Irradiance, and concentration.
Page 5: Minimum System Requirements
4 GB or higher Hard Disk 100 MB or higher Screen Resolution 1024 x 768 or higher Operating System Windows 10, Windows 11 Software Microsoft .NET Framework 3.5* IMPORTANT： Versions other than Microsoft .NET Framework 3.5 will not work. https://www.als-japan.com...
Page 6: Installing Sec Spectra
"Manual" and "SEC Spectra _ ** (software installer)". Open the "SEC Spectra_**" folder and double-click "setup.exe" to start the program installation. The setup wizard starts up. On the Welcome screen(Figure 3-1), click Next to proceed the installation. Figure 3-1. Setup Wizard -- Welcome https://www.als-japan.com...
Page 7 Installing SEC Spectra On the Select Installation Folder screen, select Everyone or Just me and click Next. Figure 3-2. Setup Wizard -- Select Installation Folder On the Confirm Installation screen, click Next. Figure 3-3. Setup Wizard -- Installation Complete https://www.als-japan.com...
Page 8 Figure 3-4. Setup Wizard -- Installation Complete When you see the Installation Complete window(Figure 3-5), it means that SEC Spectra has been successfully installed. Click Close to finish. Figure 3-5. Setup Wizard -- Installation Complete https://www.als-japan.com...
Page 9: Installing Device Driver
The driver may not be installed due to unexpected errors. Figure 3-7 shows an example when the device is not recognized correctly. It will be recognized as a device called "SCAN" with a "!" mark. Figure 3-7. when the device is not recognized correctly In that case, you need to install the driver manually. https://www.als-japan.com...
Page 10 Figure 3-8. Select "Update driver" in Device Manager Select "Browse my computer for driver software" and manually specify the folder containing the "TAURUS" device driver and update the driver. Figure 3-9. Select "Browse my computer for driver software" in Update Drivers https://www.als-japan.com...
Page 11 The default path will be: C:¥Program Files¥ALS¥SEC Spectra¥Driver¥Taurus (32-bit systems) C:¥Program Files (x86)¥ALS¥ SEC Spectra ¥Driver¥Taurus (64-bit systems) When the driver is updated successfully, the following screen will be displayed, and click "Close" to complete the manual installation of the driver.
Page 12: Removing Sec Spectra
If your PC is connected to the network, open the "DotNetFX35" folder in the USB memory, click on the file "dotnetfx35.exe", and follow the on-screen instructions to install the .NET Framework 3.5. Figure 3-13. Installing .NET Framework 3.5 components After the installation is complete, click Close to finish the installation. https://www.als-japan.com...
Page 13 Windows system folder (C: ¥ Windows). If the message "Administrator privileges are required" is displayed *, click Continue. Figure 3-15. Copy files into a Windows system folder Figure 3-16. Click "Continue" Note: If you have any questions about "Administrator authority", please contact your system administor. https://www.als-japan.com...
Page 14 ② Right-click the file "OffLine Install NET35.bat" in the "OffLine NET35" folder and select Edit. Figure 3-17. Open file from editCopy the second line. Figure 3-18. Copy the second line. ③ Right click on "Start " icon , click on "Command Prompt (Admin)". Figure 3-19. Open "Command Prompt" https://www.als-japan.com...
Page 15 ⑥ The message "Operation completed successfully." is displayed and the offline installation is complete. Close the screen and exit. ⑦ Now, you can install SEC Spectra software. Note: Please also refer to the official Microsoft website for installing .NET Framework 3.5. https://www.als-japan.com...
Page 16: Getting Started With Sec Spectra
4-1-1. If you connect the spectrometer first… If you connect the spectrometer to your computer before starting SEC Spectra, the Spectrum_0 will be displayed automatically under the default settings of the connected spectrometer. Figure 4-2. The spectrometer is connected when the SEC Spectra is started https://www.als-japan.com...
Page 17 Next, connect the spectrometer to the computer. SEC Spectra initializes the device. Measurement is possible when initialization is completed. When the following screen appears, it means that SEC Spectra is ready for measurement. Figure 4-4. SEC Spectra is ready for measurement https://www.als-japan.com...
Page 18: Spectrum Measurement
For details of the method of setting the measuring device, please refer to "SEC2020 Spectrometer System User's Guide - Device -". 5-2. Creating a Spectrum Measurement...
Page 19 Then, enter an integration time (default: 50 ms, i.e. the exposure time for the light sensor) and a wavelength range to display on the X-axis (default: 200 nm~1025 nm, the range in the parentheses indicates the range supported by the selected device). Then, click Next. Figure 5-2. New Spectrum - Live Spectrum Settings https://www.als-japan.com...
Page 20 To specify a color, click the color patch to the right of Graph Color and select the desired color. You may simply leave the default names unchanged. When all settings are done, click OK. Figure 5-3. New Spectrum - Display Settings https://www.als-japan.com...
Page 21: Change Spectrum Acquisition Settings
Set the number of scans to calculate the average value. The higher the value, the lower the variation of the curve. Boxcar Smooths the spectrum curve. Sets the number of data to be used for the moving average calculation. The higher the value, the smoother the curve and the less prominent the changes. https://www.als-japan.com...
Page 22: Graph Toolbar
5-8. Print and Preview of Spectrum If you want to print the current spectrum, use the Print Preview and Print icons on the toolbar at the top of the spectrum diagram. Please refer to "14-9-3. Print Preview and Print" for further information. https://www.als-japan.com...
Page 23: Absorbance Measurement
6. Absorbance Measurement 6. Absorbance Measurement Absorbance measurement using the SEC2020 spectrometer system and SEC Spectra is calculated from the spectrum of light that has passed through the reference object (inject blank solvent into the cuvette) and the object to be measured (inject sample solution into the cuvette). Therefore, to measure the absorbance, first measure the transmitted light spectrum of the reference object (baseline measurement), then set the object to be measured and measure the transmitted light.
Page 24 Suggested peak value range on the window. In figure 6-3, the peak value is set within the range of 40000-60000. If the adjustment does not work, you can press Auto Configuration to set it automatically. https://www.als-japan.com...
Page 25 If the Boxcar width value is set too high, the resolution will decrease and it will not be possible to separate adjacent peaks, so set an appropriate value for your experiment. After setting all the data acquisition parameters, click Next. https://www.als-japan.com...
Page 26 (as such, please don't put the test object in place). Once the reference spectrum is captured, you can check the spectral data in the preview at the bottom of the screen. Then click Next. Figure 6-5. New Absorbance Measurement - Reference Spectrum https://www.als-japan.com...
Page 27 10 is used, instead of the default 1. Once the dark spectrum is acquired, you can confirm the acquired spectrum in the preview at the lower half of the window. Also, "Acquiring Dark Spectrum ..." is displayed while acquiring the dark spectrum. https://www.als-japan.com...
Page 28 Next, you can specify a name for the measurement graph window, as well as a spectrum title and a color for the graph. To specify the color, you can click on the color patch and select the desired color from the palette. Figure 6-7. New Absorbance Measurement - Display Settings https://www.als-japan.com...
Page 29: Absorbance Of Sample
Figure 6-8. Newly created absorbance measurement window 6-2. Absorbance of Sample After setting the absorbance measurement, set the sample to be measured in the cuvette holder. The figure below is an example of absorbance measurement of sample solution. Figure 6-9. Absorbance of methylene blue solution https://www.als-japan.com...
Page 30: Graph Toolbar Icons
A (absorbance) represents the light absorbed by the sample, and T (transmittance) represents the light passed through the sample, so the two are contradictory. In addition, T (transmittance) and R (reflectance) are similar, because R (reflectance) measures the reflected light, which is the light https://www.als-japan.com...
Page 31 Basically, whenever a measurement needs a reference or dark spectrum to work, the Set as reference spectrum icon or Set as dark spectrum icon will appear on the toolbar. This is the same for absorbance, transmittance and reflectance measurement. https://www.als-japan.com...
Page 32: Saving Absorbance Measurement
Click the "Save selected spectrum as..." icon on the graph toolbar to save the spectrum curve. Figure 6-17. Saving absprbance spectrum by Save selected spectrum as... icon https://www.als-japan.com...
Page 33 If you delete any of the curves, the software will not be able to display the absorbance correctly. 6-4-4. Print and Preview of Absorbance Spectrum Curve If you want to print the spectrum of the absorbance spectrum, use the "Print Preview" "Print" icons on the toolbar. For details, refer to "14-9-3. Print Preview and Print". https://www.als-japan.com...
Page 34: Transmittance Measurement
Set the Reference spectrum and Dark spectrum. On the Display Settings screen, set the Window name, Spectrum title, and Graph color. When you have finished the display settings, click OK. The measurement window is displayed as shown in Figure 7-2. https://www.als-japan.com...
Page 35: Transmittance Of Sample
Figure 7-2. Newly created transmittance measurement window 7-3. Transmittance of Sample Next, set the object to be measured and measure the transmittance. The result of measuring the sample is displayed in the figure 7-3. Figure 7-3. Transmittance of methylene blue solution https://www.als-japan.com...
Page 36: Saving Transmittance Measurement
Open Spectrum File from the File menu. For details, refer to "14-1-1. Open Spectrum File". If you want to print the spectrum of the transmittance spectrum, use the Print Preview and Print icons on the toolbar. For details, refer to "14-9-3. Print Preview and Print". https://www.als-japan.com...
Page 37: Reflectance Measurement
Set the Reference spectrum and Dark spectrum. On the Display Settings screen, set the Window name, Spectrum title, and Graph color. When you have finished the display settings, click OK. The measurement window is displayed as shown in Figure 8-2. https://www.als-japan.com...
Page 38: Reflectance Of Sample
Figure 8-2. Newly created reflectance measurement window 8-3. Reflectance of Sample Next, set the object to be measured and measure the reflectance. The figure below shows an example of the result of measuring the reflectance. Figure 8-3. Reflectance measurement of a sample https://www.als-japan.com...
Page 39: Saving Reflectance Measurement
Open Spectrum File from the File menu. For details, refer to "14-1-1. Open Spectrum File". If you want to print the spectrum of the reflectance spectrum, use the Print Preview and Print icons on the toolbar. For details, refer to "14-9-3. Print Preview and Print". https://www.als-japan.com...
Page 40: Strip Chart (Recording Specific Wavelength)
Strip Chart measurement, the following explanation is from the state where the "Absrbance_0" spectrum curve has been created in advance. 9-1. Creating a Strip Chart Measurement From the Measurement menu, select Strip Chart to open a New Strip Chart window: Figure 9-1. Selecting Strip Chart from the Measurement menu https://www.als-japan.com...
Page 41 Then, select the wavelength or wavelength range and press Add. If you want to add multiple wavelengths, repeat the operation selecting the desired wavelengths and press Add. Note: The software will automatically select the closest wavelength. Figure 9-2. New Strip Chart - Strip Chart Settings https://www.als-japan.com...
Page 42 Stop icon on the toolbar of the Strip Chart measurement screen that opens in the next step. You can uncheck it, enter the recording time, and set the time until it stops. Figure 9-3. Strip Chart Settings - Record Setting https://www.als-japan.com...
Page 43 Figure 9-5. The chart starts scrolling after ten minutes Note: When multiple wavelengths are added in the Strip Chart Settings, not all curves may be displayed. In this case, press the autoscale icon on the toolbar to show all curves. https://www.als-japan.com...
Page 44: Toolbar Icons
When scrolling is active, this icon has a blue frame, and when scrolling is inactive, it lacks the frame. See the following figure: Figure 9-8. The "active"(left) and "inactive"(right) states of the Strip Chart View icon https://www.als-japan.com...
Page 45 Figure 9-12. Display width of delta time 9-2-3. Reset and Stop Icon Press the reset icon to reset the acquired data of the Strip Chart. The newly imported data will be the first data. Press the stop icon to stop the Strip Chart screen. https://www.als-japan.com...
Page 46: Saving Strip Chart
Strip Chart data. A file will be formed for each wavelength measured. You can save the spectrum checked for selection at the same time. Click the save icon on the graph toolbar to save the Strip Chart data. A file is formed for each measurement https://www.als-japan.com...
Page 47: Loading Strip Chart File
Open Spectrum File from the File menu. For details, refer to "14-1-1. Open Spectrum File、14-1-2. Open Strip Chart File" . The following is an example of displaying multiple items in a New window-Same window. Figure 9-15. Displaying the StripChart results in a New window-Same window https://www.als-japan.com...
Page 48: Time Sequence Measurement (Recording Full Wanvelength)
When you move the mouse, the window closes automatically. If you want it to stay on the screen, you can click on the push-pin icon on the upper-right corner of the pane to fix it. See the following figure: Figure 10-2. The push-pin for pinning the Time Sequence window https://www.als-japan.com...
Page 49: Settings Of Time Sequence Measurement
(millisecond). In effect, recording starts as soon as you chick the Record button. Never set to zero (0) here, or there will be an error. If you want to start recording immediately by pressing Record icon, please check on Start. Figure 10-4. Setting When to start https://www.als-japan.com...
Page 50 (file type: spc), and can be played from File -> Open Time Sequence File. For details, refer "10-4. Loading and Playing Time Sequence File". Figure 10-7. Setting What to save https://www.als-japan.com...
Page 51: Recording Time Sequence Measurement
During and after recording, a green band may appear on the software screen, indicating that the spectrometer is communicating with the computer. Regardless of this indication, data will be recorded and saved according to the setting conditions. Figure 10-10. Green band display https://www.als-japan.com...
Page 52: Loading And Playing Time Sequence File
Figure 10-11. Selecting Open Time Sequence File from the File menu This opens the system standard Open window. Now locate the file you saved earlier, and then click the Open button. Figure 10-12. Selecting previously saved file in the Open window https://www.als-japan.com...
Page 53 Through playback, you can easily go back and forth to find out how the measurement reading changes. Moreover, you will notice that the time sequence file will keep playing over and over again once it is loaded until you press the Pause button. https://www.als-japan.com...
Page 54: Relative Irradiance Measurement
Set the color temperature of the reference light source. Example: It is 5500K when both the D2 and HAL lamp of the SEC2020 spectrometer system are used. It is 2800K when only the halogen lamp is used. Figure 11-2. Setting color temperature...
Page 55 When the setting is completed, SEC Spectra will display the relative irradiance measurement graph. The figure below is an example of the relative irradiance spectrum when only the halogen lamp of the SEC2022 light source is turned on. Figure 11-3. Newly created Relative Irradiance measurement window https://www.als-japan.com...
Page 56: Relative Irradiance Sample Measurement
Open Spectrum File from the File menu. For details, refer to "14-1-1. Open Spectrum File ". If you want to print the spectrum of the Relative irradiance spectrum, use the Print Preview Print icons on the toolbar. For details, refer to "14-9-3. Print Preview and Print". https://www.als-japan.com...
Page 57: Concentration Measurement
Integration time, Number of scans to average and Boxcar width. When all the parameters are set, click Next. Set the Reference spectrum and Dark spectrum. On the Display Settings screen, set the Window name, Spectrum title, and Graph color. https://www.als-japan.com...
Page 58: Concentrationsettings
(Figure 12-2), and another is to specify the parameters of Lambert-Beer's law (Figure 12-3). Figure 12-2. Concentration Settings - Calibration Table From Known Concentration Figure 12-3. Concentration Settings - Beer's Law Settings https://www.als-japan.com...
Page 59 ⑥ If you want to delete the data, select the data row in list and click Delete icon to delete the point. ⑦ Compound name and Concentration unit entered here will be displayed in the concentration measurement graph (Figure 12-5). Figure 12-4. Configuration of Concentration Settings - Calibration Table From Known Concentration https://www.als-japan.com...
Page 60 Table button. When you have finished the settings, click Apply. Once the settings have been applied, the concentration curve will be displayed in a measurement window similar to the Strip Chart, as shown below. Figure 12-5. Newly created concentration measurement graph https://www.als-japan.com...
Page 61 Wavelength: Enter the absorption peak wavelength (nm) to be measured. Once the Compound name and Concentration unit have been entered, they will be displayed as Compound Name and unit in the concentration measurement graph. Figure 12-6. Beer’s Law Settings https://www.als-japan.com...
Page 62: Start Measuring The Concentration Of Test Solution
Except for the same toolbar icons as in Strip Char, there is a button dedicated to Concentration Setting for concentration measurement. See the following figure: Figure 12-8. Concentration Setting icon Concentration Setting icon opens the window for you to specify the Calibration Table From Known Concentration and Beer's Law Settings. https://www.als-japan.com...
Page 63: Saving The Concentration Measurement
File menu. For details, refer to "14-1-2. Open Strip Chart File". The result of one concentration measurement is calculated by multiple spectral curves. Therefore, not delete any curve. If you delete any of the curves, the result will not be calculated correctly. https://www.als-japan.com...
Page 64: Trigger Mode Settings
13. Trigger Mode Settings 13. Trigger Mode Settings The SEC2020 spectrometer system supports "trigger mode". The external TTL signal applied to the spectrometer’s I/O port controls data acquisition of the spectrometer. Please refer to chapter "Synchronization with other devices" of "SEC2020 Spectrometer System User's Guide -Devices-".
Page 65 I/O port or the software settings, as data acquisition starts when the TTL signal goes to “High level”. You can check the current mode status from Spectrum List--Mode: **. The figure below shows the state set for SW Continuous Trigger mode. Figure 13-3. Spectrum List-- Mode: SW Continuous Trigger https://www.als-japan.com...
Page 66: Trigger Off (Normal Mode)
At this time, the signal on the spectrum screen moves and data is acquired. You can check the current mode status from Spectrum List--Mode: **. The figure below shows the state set for Trigger Off (normal) mode. Figure 13-4. Spectrum List-- Mode: Trigger Off https://www.als-japan.com...
Page 67: Software Operation Methods
File menu Figure 14-2. Open Existing File On the Display Settings screen, select Select existing window to display the spectrum in the current Spectrum_0 window. Display in new window creates a window separate from Spectrum_0. Press OK. https://www.als-japan.com...
Page 68 The figure below shows the result of opening Spectrum_1 when the wavelength interval is set to 10 nm and Spectrum_0 when the default setting is set at the same time. The spectra are overlay in this way, making it easy to compare spectral differences. Figure 14-4. Select existing window to display the spectrum https://www.als-japan.com...
Page 69 Figure 14-5. Open multiple spectrum files On the Display Settings screen, check Same Window under Display in new window. If you do not check Same Window, a window will be created for each file. Figure 14-6. Display in new window -- Same Window https://www.als-japan.com...
Page 70 Click File -> Open Spectrum File in the same way that you open a spectrum file. On the Display Settings screen, select Show in New Window or Select Existing Window to display the spectrum. Figure14-8. Display the saved Strip Chart in New Window--Same Window https://www.als-japan.com...
Page 71 Select Close All Measurements from the File menu. Figure 14-9. File -- Close All Measurements 14-1-5. Page Setup and Print Press Print Preview to open the Print preview window and display the current from the File menu spectrum diagram. Figure 14-10. Print Preview window https://www.als-japan.com...
Page 72 Print button on SEC Spectra's graph toolbar to open the standard Print window: Figure 14-11. Print window In addition, the File menu also provides a Page Setup option. This feature lets you select the desired paper, orientation, and margins. Figure 14-12. Page Setup window https://www.als-japan.com...
Page 73 14-1-7. Device Information If you need to view detailed information about a connected device, go to the File menu, and then select Device Information, as shown in the following figure: Figure 14-14. Selecting Device Information from the File menu https://www.als-japan.com...
Page 74 SEC Spectra User's Guide 14. Software Operation Methods Then, the following window appears, showing details about the device: Figure 14-15. Device information details https://www.als-japan.com...
Page 75: View Menu
Device List shows the basic attributes of connected devices, such as models, serial numbers (SN), and wavelength ranges. Note: The serial number is how SEC Spectra identifies each device. This number is also used in several windows where device selection is required. https://www.als-japan.com...
Page 76 "Device User's Guide, 3-2-2 Automatic Mode". When you move the mouse cursor from View->Light Source menu or on the vertical Light Source icon on the right edge of the SEC Spectra window, the light source frame is displayed. Figure 14-19. Light Source icon https://www.als-japan.com...
Page 77 You can also adjust the size of the font for the user interface. Go to the View menu, and then select Font. You can choose the font size that best fits your needs. Figure 14-21. Selecting Font from the View menu https://www.als-japan.com...
Page 78: Application Settings
Default Settings allows you to change the default setting for each measurement (spectrum, absorbance, transmittance, reflectance and relative irradiance). The settings must be made for each measurement. Default Settings tab of Application Settings Figure 14-23. Measurement Set the default display of the measurement selected from the pull-down on the right side. https://www.als-japan.com...
Page 79 Therefore, it is necessary to adjust the total time to be short until the warning message disappears. https://www.als-japan.com...
Page 80 You can select one or more file formats to save the spectrum, absorbance, transmittance, reflectance, and relative irradiance measurement spectrum from sps, csv, and txt. You can select one or both file formats to save records in Strip Chart measurement from spt and csv. https://www.als-japan.com...
Page 81 This controls the line width of the curve on a graph. Window Background and Chart Background These two control the background styles and colors of the measurement window and the measurement graph, respectively. The available styles are None, Brush, and Solid. https://www.als-japan.com...
Page 82 This allows you to show the visible spectrum on the graph along with the measurement curve. Figure 14-27. Demonstrating Human Visble Spectrum effects in the Application Settings Note: For these graph settings, you can click "Apply" to see the effect of a change immediately. https://www.als-japan.com...
Page 83 Here you specify whether to use the Savitzky-Golay filter. To use it, select the Enable filter check box, and then specify the smoothing Degree and the smoothing Mode. The available modes are Reference Curve, Reference & Measurement Curve, and Measurement Curve. https://www.als-japan.com...
Page 84 Here you specify whether to use the Kalman filter. To use it, simply select the Enable filter check box. Long Pass Filter Here you specify whether to use the long pass filter. To use it, simply select the Enable filter check box. Note: Click "Apply" to see the effect immediately after you change these smoothing settings. https://www.als-japan.com...
Page 85: Default Layout
Window menu and select Default Layout to revert to their default layout: Figure 14-29. Selecting Default Layout from the Window menu The window layout now reverts back to the way it originally was: Figure 14-30. The default window layout of the program https://www.als-japan.com...
Page 86: Quick Adjustment Field
The sensitivity of the spectrometer CCD is not linear with respect to intensity. When the linearity correction function is enabled, the correction is performed according to the linearity correction table. For details, refer to "15-2. Linearity Correction". Relative Irradiance Start relative irradiance measurement. For details, refer to "11. Relative Irradiance Measurement". https://www.als-japan.com...
Page 87: Graph Toolbar Icons
This icon automatically adjusts the X- and Y- axes to their optimal settings. The following figure shows the effect of this icon. In the figure, the X-axis is adjusted to display the entire wavelength range supported by the device, while the Y-axis is adjusted so that the curve fills the entire graph as much as possible: https://www.als-japan.com...
Page 88 This icon automatically adjusts the scale of Y-axis to show more details. The following figure shows the effect of this button. In the figure, the highest point on the curve is extended to the top of Y-axis: Figure 14-37. The effect of using Scale Y-axis automatically https://www.als-japan.com...
Page 89 This icon enlarges the graph (pulling it closer to you). Here we press Scale graph axes automatically to restore the graph to its optimal display settings, and then press Zoom In to demonstrate its effect. The following figure shows the result of pressing Zoom In three times: https://www.als-japan.com...
Page 90 This icon shrinks the graph (pushing it away from you). Here we first zoom in on the graph three times, and then press Zoom Out twice to demonstrate its effect. See the following figure: Figure 14-43. The effect of pressing Zoom Out https://www.als-japan.com...
Page 91 This icon restores the graph display settings to application defaults (i.e. the original settings when the graph is created). To demonstrate, we press this button to restore the graph in the former example to its default settings. See the following figure: Figure 14-45. The effect of Reverting graph settings to application defaults https://www.als-japan.com...
Page 92 200 to 1025 (nm), with a major step of 100. Then, we configure the Y-axis (signal intensity) range as 0 to 50000, with a major step of 5000. See the following figure for the result: Figure 14-48. Using custom X-, Y-axes settings https://www.als-japan.com...
Page 93 Figure 14-49. Checking the value of a specific wavelength with mouse The mouse cursor offers a handy way to observing a single point. To display all measurement data, SEC Spectra provides a tabular "Spectral data pane". Details will be explained in chapter 14.7. https://www.als-japan.com...
Page 94 As such, it is better to click on the curve at the wavelength position where you want to observe, rather than clicking on the empty space on the graph. Otherwise, the mark may go to where the peak value appears, instead of the X position you click. https://www.als-japan.com...
Page 95: Spectrum Data Pane
Information tabs at the lower half of the pane. The Spectral Data tab shows a scrollable table and uses the scroll bar on the right to navigate to the wavelength range you want to check. Figure 14-53. Spectrum Data Pane https://www.als-japan.com...
Page 96 After specifying Wavelength and Type, click on the green "+"symbol (i.e. the Add button). A new mark will appear at the specified wavelength position. Note: The makeable positions of SEC Spectra are located at specific intervals. As a result, the program automatically places the mark at the position closest to the specified wavelength position. https://www.als-japan.com...
Page 97 Statistics tab and read the required information. In the figure below, the table reads the current average, minimum, maximum and center of gravity. Figure 14-56. Statistics Information tab https://www.als-japan.com...
Page 98: Saving Currrent Spectrum
To save in CSV or TXT format, select csv files (extension .csv) or text files (extension .txt) in Save as type in the Save As window. Note: CSV or TXT format can only be used for other software. SEC Spectra can not read spectrum curves in CSV or TXT format. https://www.als-japan.com...
Page 99 Next, the Browse for Folder window will appear, select the destination folder, and then press OK when you have made your selection. All spectrum data files are saved in the specified folder. The file name is the title of the spectrum and the file extension is .sps. In this example, the saved file is "Spectrum_0.sps". https://www.als-japan.com...
Page 100 You can specify a name for the temporary log spectrum or simply use the default number as the name. For example, change the number to "intg30" on condition that the integration time is 30 ms. Figure 14-63. Renaming the temporary log spectrum https://www.als-japan.com...
Page 101 First, in the Spectrum list of the Spectrum Data pane, select the spectrum you want to delete, right-click to open the pop-up menu and select Delete. This will delete the selected spectrum curve. Figure 14-65. Selecting Delete on the pop-up menu https://www.als-japan.com...
Page 102: Other Tool Bar Icons
SEC Spectra provides a feature to automatically identify the peaks and show their values on the graph. Here is the icon to show the peak values: Figure 14-67. Peak icon When pressed, the Peak button opens the Peak Information window: Peak Information window Figure 14-68. Peak Information window https://www.als-japan.com...
Page 103 Figure 14-71. Print Preview icon The same window opens as Print and Print Preview from File menu (14-1-5. Page Setup and Print) .Pressing the Print Preview icon displays a standard Print Preview window, which shows the current spectrum measurement. https://www.als-japan.com...
Page 104 Figure 14-73. The effect of using the Set integration time automatically icon The integration time has changed from 50 ms to 13 ms after the Set integration time automatically button is pressed. And the spectrum is corrected slightly downward. https://www.als-japan.com...
Page 105 SEC Spectra offers the function of displaying FWHM in real time. You can click the FWHM icon and click on any wavelength of the spectrum. The program will automatically find the highest peak nearby and measure its FWHM and reflect it in the spectrum. Figure 14-75. FWHM icon https://www.als-japan.com...
Page 106 As you press this icon, all data in the spectrum data pane will be copied to the system clipboard. You can then paste the data into the application you want. For illustration, we paste the data into Windows Notepad in the following figures: Figure 14-77. Pasting spectrum data into Windows Notepad https://www.als-japan.com...
Page 107 In most cases, SEC Spectra acquires data continuously from the spectrometer. Clicking the Save icon will activate the Pause icon and pause continuous acquisition. If you want to resume the measurement, press Start Acquiring icon to return to continuous acquisition. Figure 14-79. Start Acquiring icon and Pause icon https://www.als-japan.com...
Page 108: Spectrum Correction Features
When the electronic dark correction is enabled in the SEC Spectra software, the dark current will be dynamic removed from the measurement curve. The following figures show two different measurement curves without and with electronic dark correction, respectively: Figure 15-1. Without electronic dark correction https://www.als-japan.com...
Page 109 Figure 15-2. With electronic dark correction To enable electronic dark correction, click on the Electronic Dark Correction button above the toolbar. To disable it, just click on the button again. See the following figure: Figure 15-3. Electronic Dark Correction icon https://www.als-japan.com...
Page 110: Linearity Correction
Figure 15-5. With linearity correction To enable linearity correction in SEC Spectra, click on the Linearity Correction icon above the toolbar. To disable it, just click on the button again. See the following figure: Figure 15-6. Linearity Correction icon https://www.als-japan.com...
Page 111: Contact Information
SEC Spectra as a new user. If you have any further questions about SEC Spectra or any suggestions to this guide, please contact us at: Manufactured by: ALS Co., Ltd Exclusive distributor: BAS Inc. Address: 1-28-12, Mukojima, Sumida-ku, Tokyo 131-0033, JAPAN...

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