Summary of Contents for Munro AW300SG
- Page 1 ANAEROBIC WORKSTATION AW300SG MANUAL PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION Burnt Mill, Elizabeth Way Harlow, Essex, CM20 2HU UK 44-45 met@munro-group.co.uk MUNRO.6.22...
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Page 2: Table Of Contents
CONTENTS GENERAL ARRANGEMENT DRAWING OF ANAEROBIC WORKSTATION GENERAL SPECIFICATION GAS SUPPLIES ELECTRICAL SUPPLES INSTALLATION OF GAS AND ELECTRIC DRAWING ASSEMBLY INSTRUCTIONS DRAWING CHECKING THE INCUBATOR ATMOSPHERE FOR OXYGEN PREPARING THE ANAEROBIC INDICATOR SOLUTION COMMISSIONING THE CABINET 10. CHECKING THE GAS FLOW 11. -
Page 3: General Arrangement Drawing Of Anaerobic Workstation
GENERAL ARRANGEMENT OF AW300SG ANAEROBIC CABINET Removable lock lid Spotlight Humidistat Power socket Removable exterior port covers complete with retaining clips 26" (660mm) Condensation drain bottle 3"(76mm) 30" (762mm) 33" (838mm) Heating chamber Petri dish entry lock Bare hand operation ports... -
Page 4: General Specification
GENERAL SPECIFICATION MODEL AW300SG MAIN CHAMBER INCUBATION CAPACITY 300 Petri Dishes DOOR SEALING Manual Mixed Gas (10% H2 + 10% CO2 + 80% N2) OXYGEN REMOVAL FROM AIRLOCK Positive Pressure flushing FABRICATION MATERIAL Acrylic OVERALL DIMENSIONS Length 33" 838mm Depth 29"... -
Page 5: Gas Supplies
GAS SUPPLIES This Anaerobic Cabinet requires one gas cylinders fitted with a two stage regulator. The output side of the regulator should have a range of 0 - 50 p.s.i. (3.3 bar). The gas cylinder should contain: 10% Hydrogen + 10% Carbon Dioxide + 80% Nitrogen CAUTION: GAS CONTAINING MORE THAN 10% HYDROGEN SHOULD NOT BE USED UNDER ANY CIRCUMSTANCES... -
Page 6: Installation Of Gas And Electric Drawing
INSTALLING THE GAS AND ELECTRICAL SUPPLY TO THE AW300SG WORKSTATION 2 stage pressure regulator Set outlet pressure sufficient to maintain pressure at inlet to cabinet Primary gauge showing pressure in bottle On/Off tap Electrical supply 230V AC 50Hz 6mm diameter flexible... -
Page 7: Assembly Instructions Drawing
AW300SG ASSEMBLY INSTRUCTIONS DRAWING Fit the indicator pump on hook provided and plug into this socket Air Exhaust Air Inlet Powder Bung Sleeve assy. 'O' ring Port Cover Place the conditioning sachet then the catalyst in the channels Conditioner Catalyst under the tray in the order shown. -
Page 8: Checking The Incubator Atmosphere For Oxygen
CHECKING THE INCUBATOR ATMOSPHERE FOR OXYGEN The Anaerobic Indicator Solution will indicate traces of Oxygen in the incubator atmosphere by changing colour from being clear when the atmosphere is anaerobic to being blue when oxygen is present. The Indicator Solution is held in a small bottle located in the bottom of the incubator and is held in position by a plastic holder. -
Page 9: Commissioning The Cabinet
COMMISSIONING THE CABINET Check all the following points before starting to commission the workstation. Switch "OFF" the cabinet at the Mains ELCB switch. The gas flow has been set at the factory to 35 litres per minute. However during initial commissioning please check this gas flow as described on page 10 by placing a gas flow meter and pressure gauge in the gas line. -
Page 10: Checking The Gas Flow
CHECKING THE GAS FLOW To check the Gas Flow into the Workstation the following equipment is required: 1. Gas Flow Meter (0 – 50 Litres per minute) 2. Pressure Gauge (0 – 3 Bar) Connect up the equipment as shown and lift lid as shown. Gas Out Flow Meter Pressure Gauge... -
Page 11: Working Inside The Cabinet With Bare Hands
WORKING INSIDE THE CABINET WITH BARE HANDS The inside of the sleeve is always held at positive pressure by the Gas Pressure Switch, therefore if the Bung is removed from the Cuff, gas will flow out of the cabinet and no oxygen will enter. To insert the hand into the cabinet, firstly remove both port covers by placing the neoprene retainers on each side of the port as shown and put the covers in a safe place. -
Page 12: Sleeve Assembly
SLEEVE ASSEMBLY The Most likely part of the sleeve assembly to suffer damage is the cuff. This can be replaced by following the diagrams shown below. The assembly consists of four parts:- 1. The sleeve 2. The rigid cuff 3. The wrist cuff 4. -
Page 13: Removal Of Condensation From Inside The Incubator
THE REMOVAL OF CONDENSATION FROM INSIDE THE INCUBATOR Water vapour accumulates inside the incubator atmosphere from two sources, firstly from the evaporation of the media in the Petri dishes and secondly by the hydrogen and the traces of oxygen being linked together by the palladium catalyst. -
Page 14: Transferring Items Into The Incubator
TRANSFERRING ITEMS INTO THE INCUBATOR The workstation is supplied with four aluminium petri dish holders, each capable of holding ten dishes. These are used for transferring dishes into and out of the cabinet and for stacking them whilst they are being incubated. -
Page 15: Changing The Catalyst And Conditioner
the cabinet then it passes under the bottom shelf of the incubator where it first meets the sachets of Conditioner and then the sachets of Catalyst. The gas now gives up just sufficient of its heat to maintain the required temperature before finding its way back to the top of the incubator due to the suctional force of the fan. -
Page 16: Test Record Sheet
TEST RECORD SHEET MODEL AW300SG SERIAL NO 7390 CUSTOMER PUNMICRO MIXED GAS FLOW 35 L / MIN MIXED GAS INPUT PIPE SIZE 6 mm DIA INCUBATOR POSITIVE PRESSURE SETTING 6 mm WG INCUBATOR LEAK NOT DETECTED 1 HOUR TEMPERATURE SETTING 36.5 deg C... -
Page 17: Declaration Of Conformity
Declaration of Conformity Anaerobic Incubator type AW300SG Equipment Manufacturer MUNRO Ltd Address urnt Mill, Elizabeth Way Harlow, Essex, CM20 2HU UK United Kingdom ,44-45 Description of Equipment Incubator for the environmentally controlled incubation of anaerobic bacteria I certify that the apparatus identified above conforms to the requirements of Council Directive...
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