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Summary of Contents for C.B.S. SCIENTIFIC EBU-4000
- Page 1 INSTRUCTION MANUAL Semi-Dry Blotting Systems EBU-4000 EBU-6000...
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Page 2: Table Of Contents
TABLE OF CONTENTS Page Important User Information Section 1 General Information Introduction Specifications Safety Section 2 Description of parts Unpacking EBU-4000 or 6000 Components/Assembly Section 3 Instructions for Use Blotting Unit Preparation 7-11 Section 4 References Section 5 Maintenance of Equipment... -
Page 3: Important User Information
This product was produced utilizing the highest practical standards of materials, workmanship, and design. C.B.S. Scientific warrants that the product has been tested and will meet or exceed published specifications. This warranty is valid only if the product has been operated and maintained according to the instructions provided. - Page 4 Handhabung, Veränderung des Produktes durch den Benutzer, oder unzureichende Leistungen die sich risultato di condizioni al di fuori del controllo del fabbricante. La C.B.S. Scientific non sarà in ogni caso nicht im Verantwortungsbereich von C.B.S. Scientific befinden. C.B.S. Scientific kommt unter keinen responsabile per danni incidentali o consequenziali, incluso, senza limitazioni, perdite di profitto, perdita Umständen für folgende Schäden auf:...
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Page 5: General Information
These blotting systems have transfer capabilities for gel dimensions up to 20cm x 20cm (Cat. # EBU-4000) or 35cm x 45cm (Cat. # EBU-6000). The stainless steel cathode and platinum-coated titanium anode are durable and corrosion-resistant. -
Page 6: Description Of Parts
Section 2 Description of Parts Unpacking the EBU-4000 or EBU-6000 Please verify that your unit comes complete with the following components: • Blotting chamber with stainless steel cathode, and platinum-coated anode • Safety cover with power leads • Mylar sheets (for shielding uncovered portion of anode) -
Page 7: Instructions For Use
0.8mA/cm of gel area. For gels used in the EBU-4000 (15x15cm up to a max. of 20x20cm) this translates to upwards of 300mA. In the larger EBU-6000, this can increase to 1200mA. Please ensure that an adequate power supply is being used to power the transfer. - Page 8 C.B.S. has designed this device such that it cannot be opened unless the power cords are disconnected. DO NOT attempt to open the transfer apparatus without disconnecting the power cords and shutting off the power supply. C.B.S. Scientific Co, Inc. Semi-Dry Blotters...
- Page 9 Place 3-4 pieces of saturated filter paper carefully centered over the cutout. Flatten the filter paper by rolling a glass rod over the surface. C.B.S. Scientific Co, Inc. Semi-Dry Blotters...
- Page 10 Standard electrophoresis buffers for RNA are usually 1XTAE or 1XTBE. Electro-blotting transfer occurs in low ionic strength buffer. Exchange the 1X electrophoresis bufferby soaking in 0.1X buffer for 20-30 minutes. 3. Preparation of the Apparatus C.B.S. Scientific Co, Inc. Semi-Dry Blotters...
- Page 11 Carefully lift the lid and watch for filter paper sticking to the top electrode. Remove the Nylon membrane and process according to protocol. Rinse the inside of the apparatus and the mask with ddH C.B.S. Scientific Co, Inc. Semi-Dry Blotters...
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Page 12: References
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 18.47-18.61. Towbin, J., Staehelin, T., Gordon, J., Electrophoresis transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications, PNAS, USA, Vol. 76, 4350-4354 (1979). C.B.S. Scientific Co, Inc. Semi-Dry Blotters... -
Page 13: Maintenance Of Equipment
(e.g. cracks, nicks, abrasions, or melted insulation), replace them immediately. • The platinum wire is secured to the banana jack by compression between a stainless washer and the jack nut. The nut/washer interface should be tight and free of corrosion. C.B.S. Scientific Co, Inc. Semi-Dry Blotters... -
Page 14: Equipment And Accessories
Semi-Dry Blotting System. Includes power leads and safety interlock, 20cm x 20cm. EBU-6000 Semi-Dry Blotting System. Includes power leads and safety interlock, 35cm x 45cm. MM-4000 Mylar® Mask for EBU-4000, package of 4 each MM-6000 Mylar® Mask for EBU-6000, package of 6 each C.B.S. Scientific Co, Inc. - Page 15 NOTES...
- Page 16 Fax: 858-755-0733 Check out our new and improved online ordering! www.cbsscientific.com E-mail address: sales@cbssci.com Mailing address: C.B.S. Scientific Company P.O. Box 856 Del Mar, CA 92014 Shipping address: C.B.S. Scientific Company 10805 Vista Sorrento Pkwy Suite 100 Solana Beach, CA 92121...