anvajo fluidlab R-300 User Manual
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anvajo fluidlab R-300 User Manual

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Summary of Contents for anvajo fluidlab R-300

  • Page 1 R-300 User manual...
  • Page 2 All the contents of this user manual, in particular text, photographs and graphics, are protected by copyright. Unless expressly stated otherwise, the copyright is held by anvajo GmbH. Please ask us if you wish to use the contents of this user manual elsewhere.
  • Page 3 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

  • Page 4: Table Of Contents

    Software Update ......................24 Maintenance ........................25 Language Settings......................25 Displaying system information ..................26 Carrying Out Measurements ................... 27 Preliminary Remarks ...................... 27 Spectrometric Measurements with the fluidlab R-300 .......... 27 6.2.1 General .......................... 27 6.2.2 Using the Cuvettes ..................... 27 6.2.3 Extinction ........................
  • Page 5 Quality Assurance ......................52 6.5.1 Sensor Status ....................... 52 6.5.2 Refresh Sensor Data ....................53 anvajo datalab ........................55 Connection to the fluidlab R-300 ................55 7.1.1 Connection via WLAN ....................55 7.1.2 Connection via Hotspot .................... 55 Data Export ........................56 Updates via datalab......................

  • Page 6: Safety Instructions/Hazard Analysis

    User Manual fluidlab R-300 Safety Instructions/Hazard Analysis Symbols Used in the User Manual and on the Device Symbol Meaning Warning! Please observe the warnings and precautions in this manual to enable you to use the product safely. Follow the instructions for use.

  • Page 7: General Safety Instructions

    Each user is responsible for complying with the safety and health regulations and determining and observing any restrictions before using the device. These restrictions include avoiding the use of hazardous substances. Please notify anvajo GmbH if any malfunctions, faults or damage to the system are observed during operation. Warning! To avoid the risk of electric shock, the system must be operated in its original condition, closed on all sides.

  • Page 8: Electromagnetic Considerations

    An LED is used during fluid measurements / reference measurements. Avoid looking directly into the active LED, as this may damage the retina. Warning! If the device is used in a manner not specified by anvajo GmbH, the protection supported by the device may be impaired. Electromagnetic Considerations The system is intended for use in an industrial electromagnetic environment.

  • Page 9: Lithium Polymer Battery

    WLAN connection. Furthermore, in such cases a decrease in the data transfer rate may be expected. anvajo GmbH will not be responsible for the loss, interception or misuse of data sent or received when using the device's wireless communication methods.

  • Page 10: Protection Against Ultraviolet Radiation (Uv)

    Warning! The device shall only be used with the accessories specified by anvajo GmbH. As far as possible, the slider should be closed during the measuring process. The device must be switched off while the measuring chamber shaft is being cleaned.
  • Page 11 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

  • Page 12: Introduction

    User Manual fluidlab R-300 Introduction A Brief Description and Intended Purpose The fluidlab R-300 is a compact and versatile fluid analysis device which has been developed for research applications, with which a large variety of liquids can be analysed quickly,...

  • Page 13: Overview

    Robust and fast image signal processing and data-parallel operations, combined with a high level of computing power, make efficient analysis of microscopic images possible directly in the fluidlab R-300 (local processing, no cloud service required). In addition, machine learning methods, such as deep neural networks, guarantee accurate counting and classification of the optical outputs with immediate quantitative results.

  • Page 14: Product Set Components

    Please check that the product set is complete, based on the delivery note. Only products and accessories that are completely free of defects should be put into operation. Damage must be reported immediately to anvajo GmbH or its representatives. Note: Please note the full part number (see chapter 9.2) as specified on the delivery note...

  • Page 15: Description Of Device

    Figure 2: Detailed description of device. Name Description USB-C socket Port for the charging cable Power on/off button Switches the fluidlab R-300 on or off Touch screen Interface for interaction with the device Protects the measuring chamber (shown open in the Slider illustration)

  • Page 16: Setup And Use

    Setup and Use Note: If used as a portable device, the fluidlab R-300 must be held in one hand and operated with the other free hand. If it is being used as a desktop device or when it is being charged, it should be placed on a stable, level, secure surface to prevent it from falling over and being damaged.

  • Page 17: Switching On The Device

    Switching On the Device Note: In order to be able to switch on the fluidlab R-300, either the device battery must be charged (partially or fully) or the USB charger must be connected to the device with the USB cable (see Chapter 4.1).

  • Page 18: Exiting Energy-Saving Mode

    Display is dimmed after 2 minutes and then switched off • Power on/off button (Figure 2-2) lights up cyclically (“pulsating”) To “wake up” the fluidlab R-300 from power-saving mode and return it to the active state, proceed as follows: 1. Tap on the touch screen.
  • Page 19 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

  • Page 20: Setting Up The System

    User Manual fluidlab R-300 Setting up the System Settings Menu The settings menu can be called up at any point on the fluidlab R-300 by tapping the menu icon ( ). The fluidlab R-300 is supplied pre-configured, which means that it is not absolutely necessary to configure the settings.

  • Page 21: Configuration Of The Wlan Adapter

    R-300. The fluidlab R-300 has a built-in WLAN adapter, which can be operated in different modes that enable various functions. In the configuration, you can choose between the following modes: A) WLAN mode: Connect your fluidlab R-300 to an existing wireless network (WLAN) in order to establish connections, e.g.

  • Page 22: Hotspot Mode

    In this case, please contact your network administrator in advance. 5.2.3 Hotspot Mode The following section describes how you can allow your fluidlab R-300 to create its own WLAN (hotspot). You can then add other WLAN-enabled devices to this WLAN so that they can communicate with your fluidlab R-300.

  • Page 23: Offline Mode

    WLAN or hotspot function has been enabled. 1. Open the settings menu of the fluidlab R-300 by tapping the menu icon ( 2. Tap on the switch to the right of “WLAN” so that it is greyed out. When the switch is greyed out, WLAN mode is disabled.

  • Page 24: Maintenance

    7. The installation is complete once the system has automatically restarted. Note: If the fluidlab R-300 is connected to a WLAN with Internet access, the system will regularly and automatically check for any software updates. The fluidlab R-300 notifies you that an update is available with a green dot on the menu icon (...

  • Page 25: Displaying System Information

    User Manual fluidlab R-300 It is possible to set a different display language as follows: 1. Open the settings menu of the fluidlab R-300 by tapping the menu icon 2. Tap on the menu item “Language”. 3. Select the desired display language by tapping on the relevant name. The new setting will be applied and the display will change straightaway.

  • Page 26: Carrying Out Measurements

    Note: If the fluidlab R-300 or the sample carriers have been stored at an ambient temperature ≤ 5°C, they must be unpacked and allowed to adjust to room temperature before measurements are carried out.

  • Page 27: Extinction

    2. Open the slider on the fluidlab R-300 as described in Chapter 6.2.2. 3. Insert sample carrier adapter 02 or 03 as described in Chapter 6.2.2. 4. Select the Extinction application on the home screen of the fluidlab R-300 by tapping on it.
  • Page 28 Note: Spectral graph (375-700 nm) - The special feature for every extinction measurement with the fluidlab R-300 is that, in addition to the extinction value at the preset wavelength, the total extinction of the liquid between 375 and 700 nm is always measured.
  • Page 29 User Manual fluidlab R-300 of your liquid, even if you are interested in extinction values at multiple wavelengths. You can move the green cursor manually in the graph and thus display the extinction values of the liquid at other wavelengths. To do so, gently tap on the green point of the cursor, maintain contact with the display and move your finger slowly along the course of the graph.
  • Page 30 User Manual fluidlab R-300 13. By clicking on the the green plus key , the next extinction measurement can be performed. It is possible to (optionally) measure a blank sample again or to directly measure the next sample (Figure 8) by clicking the continue button with a green background .

  • Page 31: Kinetics

    The Kinetics application is available since software version 21.21.01 on the fluidlab R-300. Please refer to Chapter 5.3 on how to install software updates on your fluidlab R-300. Warning! An LED is used during kinetic measurements.

  • Page 32: 6.2.4.2 Determining The Reaction Kinetics Of A Liquid Sample

    2. Open the slider on the fluidlab R-300 as described in Chapter 6.2.2. 3. Insert sample carrier adapter 02 or 03 as described in Chapter 6.2.2. 4. Select the Kinetics application on the home screen of the fluidlab R-300 by tapping on it.
  • Page 33 10. Remove the blank sample from the device and insert your sample into the fluidlab R-300 in the correct orientation (Figure 11). 11. Now start the measurement of your sample by tapping on the Continue button with a green background (Figure 12).
  • Page 34 User Manual fluidlab R-300 Note: When the extinction exceeds a value of 2.5, the kinetic measurement will be aborted automatically. The device automatically saves all the measured values which were performed before the abortion. Figure 13: Sample acquisition for kinetics reaction.

  • Page 35: Calibration Curve

    1. Prepare your samples and the blank liquid in the cuvettes and make them ready for spectrometric measurement with the fluidlab R-300. 2. Open the slider on the fluidlab R-300, as described in Chapter 6.2.2. 3. Insert sample carrier adapter 02 or 03, as described in Chapter 6.2.2.
  • Page 36 User Manual fluidlab R-300 Figure 15: “Start a test” screen of the Calibration Curve application. Note: Calibration curve list - Further down you will see the list of saved calibration curves (“calibration curve list”) (Figure 15). If you read in calibration curves for different substances and save them on the device, you can call up the created curves at a later time.
  • Page 37 User Manual fluidlab R-300 Note: Depending on whether you are determining the calibration curve by means of dilution or concentration, you must enter mandatory details. These are the highest initial concentration of the sample (conc. first sample), the dilution of the series, the sample number or the concentration of each individual sample#x (Sample#x).
  • Page 38 User Manual fluidlab R-300 Note: Force origin - If you tap on the grey button (Figure 18, left) and move it to the right, the Force origin function will be activated. This forces the curve to originate from the origin of the graph. You can switch the function off by moving the button back to the left.

  • Page 39: 6.2.5.2 Sample Quantification With Created Calibration Curve

    R- 300. 3. Open the slider on the fluidlab R-300, as described in Chapter 6.2.2. 4. Insert sample carrier adapter 02 or 03, as described in Chapter 6.2.2. 5. On the home screen of the fluidlab R-300, select the Calibration Curve application by tapping on it.
  • Page 40 User Manual fluidlab R-300 Figure 19 (left): “Start a test” screen of the Calibration Curve application. Figure 20 (right): Editing menu (green bar) for the created calibration curves. Note: Editing and deleting the created calibration curves - If you select the respective curve from the “calibration curve list”...

  • Page 41: Microscopic Measurements With The Fluidlab R-300

    µL, 10 µL and 20 µL respectively. In addition, all acella sample carriers are compatible with all standard stages for subsequent manual microscopy. 1. Open the slider (measuring chamber cover) on the fluidlab R-300 (Figure 4). 2. Check that the sample carrier adapter 01 is correctly positioned in the measuring chamber (Figure 21) of the fluidlab R-300.
  • Page 42 User Manual fluidlab R-300 Warning! You should wear gloves when filling acella sample carriers, as fingerprints on the glass may result in incorrect measurements. When drawing up the sample liquid, make sure that no air bubbles form in the pipette tip.

  • Page 43: Cell Count

    3. Insert sample carrier adapter 01, as described in Chapter 6.3.2. 4. Fill the acella as described in Chapter 6.3.2. 5. On the home screen of the fluidlab R-300, select the Cell Count application by tapping on it. 6. The “Set parameters” input screen appears (Figure 22), in which you can optionally specify the Test name for the measurement, as well as the mandatory Object size and the Dilution.
  • Page 44 9 parts dilution solution. 7. Confirm what you have entered by tapping on the green tick sign 8. Insert the sample carrier into the slot on the fluidlab R-300. Note: The device shows you in which orientation the sample carrier must be inserted into the device (Figure 23).
  • Page 45 User Manual fluidlab R-300 Figure 25 (left): “Counted objects” screen of the Cell Count application. Figure 26 (right): Histogram of cell size in the Cell Count application. Note: Warning for high cell density or cell cluster – If cell density is very high or if there are many cell clusters detected in the cell suspension, the number of cells per ml will be marked in orange in the results screen.
  • Page 46 User Manual fluidlab R-300 Figure 27 (left): Section of the cell image. Figure 28 (right): Display of the cell image in full screen mode. 13. The green plus key allows you to go directly to the next measurement with the same parameter setting, which is automatically numbered consecutively.

  • Page 47: Viability

    3. Insert sample carrier adapter 01, as described in Chapter 6.3.2. 4. Fill the acella as described in Chapter 6.3.2. 5. On the home screen of the fluidlab R-300, select the Viability application by tapping on it. 6. The “Set parameters” input screen appears (Figure 29), in which you can optionally specify the Test name for the measurement and the Cell type, as well as the mandatory Object size and the Dilution.
  • Page 48 User Manual fluidlab R-300 Figure 29: “Set parameters” screen in the Viability application. Note: The device shows you in which orientation the sample carrier must be inserted into the device (Figure 23). The device will detect if the sample carrier is missing, misaligned or unauthorised and a red information screen will be displayed (Figure 24).
  • Page 49 User Manual fluidlab R-300 13. Swipe your index finger across the screen to move to different areas of the image. Figure 30 (left): Results screen for viability measurement. Figure 30 (right): Histogram of viability measurement. Figure 31(left): Section of a cell image on the results screen, Figure 31(right): Display of the cell image in full screen mode.

  • Page 50: History

    “History” in the main screen of the fluidlab R-300 (Figure 32Figure 32: ). The measurements can be sorted by date, application or in alphabetical order (Figure 33).

  • Page 51: Quality Assurance

    Checking the sensor status is a quality control procedure for maintaining the fluidlab R- 300's measuring functionality and accuracy. 1. Open the settings menu of the fluidlab R-300 by tapping the menu icon 2. Tap on the menu item “Maintenance”.

  • Page 52: Refresh Sensor Data

    The associated sensors must always be recalibrated if no detection takes place, even if the sample carrier appears to be positioned correctly. 1. Open the settings menu of the fluidlab R-300 by tapping the menu icon 2. Tap on the menu item “Maintenance”.
  • Page 53 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

  • Page 54: Anvajo Datalab

    The anvajo datalab is a desktop client which makes it possible to access and export the data stored on the fluidlab R-300 and to update the device. The datalab is available to download for free on our website www.anvajo.com. The fluidlab R-300 can be connected to any PC running the datalab software and to multiple PC's simultaneously.

  • Page 55: Data Export

    Click Updates via datalab If it is not possible to connect the fluidlab R-300 to a WLAN, you can also update it with a few more steps via the Hotspot and with the help of the datalab. Open the anvajo datalab desktop client.

  • Page 56: Operating And Maintenance Instructions

    Users should have basic language skills in English, in order to be able to understand the messages and program texts. The fluidlab R-300 has been designed in such a way that no special product training is required to operate it.

  • Page 57: Usage Location

    User Manual fluidlab R-300 Usage Location The fluidlab R-300 is designed for indoor use in accordance with the conditions specified in 9.1. It is possible to operate the device outdoors, if the conditions specified in 9.1 are adhered to. During measurement, it is necessary to keep the measuring environment consistent in order to obtain reliable results.

  • Page 58: Cleaning

    8.5.1 General The fluidlab R-300 does not require any special form of cleaning. Dirt should be removed with a lint-free cleaning cloth. Cleaning must be carried out in a dry condition. Contact with the biohazardous samples may result in infection. Hence, the components of the device associated with the biohazardous samples are potentially biohazardous.

  • Page 59: External Cleaning

    We recommend the following cleaning supplies: • Swabs for cleaning the sensor and measuring chamber (supplied; further supplies can be ordered from anvajo). • A soft, clean paper towel. • 99% isopropanol alcohol; do not use aggressive cleaning agents, such as benzene or acetone.
  • Page 60 User Manual fluidlab R-300 Warning! Do not point the spray nozzle of a cleaner directly at the fluidlab R-300’s light source. Warning! Do not use compressed air on the measuring chamber, as this may cause particles of dirt to settle on the internal measuring equipment. In this case, the device would have to be opened and cleaned by the service department.

  • Page 61: Maintenance Activities

    Always wear gloves when working with the measuring chamber. Maintenance Activities In principle, the fluidlab R-300 is maintenance-free. To determine that it is working correctly, comparative measurements can be carried out as described in Chapter 6.5. In addition to metrological checks, the fluidlab R-300 should be checked in accordance with DGUV (German Social Accident Insurance) Regulation 3 or local equivalent regulation for examination of electrical appliances.

  • Page 62: Decommissioning

    User Manual fluidlab R-300 Decommissioning If the fluidlab R-300 is not going to be used for a period of > 3 months, we recommend that the system should be stored in the delivery box to minimise damage and contamination. Before the system is placed out of service, it should be cleaned in accordance with Chapter 8.5.
  • Page 63 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

  • Page 64: Technical Specifications

    User Manual fluidlab R-300 Technical Specifications fluidlab R-300 specifications Power supply Mains voltage (for charger) 100 - 240 V Mains frequency (for charger) 50 / 60 Hz Operating voltage fluidlab R-300 Max. current consumption 1.5 A Max. power: 7.5 W Battery life (continuous measurement 1.7 h...
  • Page 65 User Manual fluidlab R-300 Transport/storage conditions Ambient temperature - 25°C to + +50°C (optimum: 20°C) Relative humidity Max. 95% at 25°C (optimum: 40 – 60%) Duration of transport / storage Max. 6 months/ max. 1 year Safety conditions Degree of pollution...

  • Page 66: Accessories

    User Manual fluidlab R-300 Accessories Name Part number Description external, type 31507W USB power supply unit 10048 (inLine ®) USB connection cable 10049 Length: 1.8 m [type A to type C] Microscope sample carrier with a acella20 sample carrier 10114 chamber height of 20 µm...
  • Page 67 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

  • Page 68: Declaration Of Conformity

    User Manual fluidlab R-300 Declaration of Conformity Declaration of Conformity In accordance with EC directives 2014/30/EU EMC Directive on the harmonisation of the laws of the Member States relating to electromagnetic compatibility 2014/53/EU RED Directive on the harmonisation of the laws of the Member...
  • Page 69 User Manual fluidlab R-300 Edition 202104 Vers.: 1.2...

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