Omegawave OMEGAZONE STD User Manual

Laser speckle blood flow imager

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210621

Laser Speckle Blood Flow Imager

OMEGAZONE

STD

USER'S MANUAL

( LSI : ver 6.1, LIA : ver 6.0)

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Page 1 210621 Laser Speckle Blood Flow Imager OMEGAZONE USER’S MANUAL ( LSI : ver 6.1, LIA : ver 6.0)
Page 2: Table Of Contents
TABLE OF CONTENTS Page ■ CAUTION ■ SUMMARY ■ HARDWARE 1. Parts name and the function 2. Setup of the stand 4. Lens adjustment 5. Polarization adjustment 6. Block diagram ■ LSI software 1. Controls and display 2. Project Configuration File 3.
Page 3: Caution
7. Do not use this instrument as a conventional computer. ＿＿＿＿＿＿＿＿＿＿＿＿＿＿ This manual is changed without a preliminary notice. There is a copyright of this manual in OMEGAWAVE, INC. A reproduction without permission is prohibited in any kind of form. Microsoft and Windows are registered trademarks of Microsoft Corporation.
Page 4: Summary
This OMEGAZONE STD consists of the laser unit, the CMOS camera, the lens + hybrid filter, the computer-based image processor and the stand.
Page 5: Hardware
HARDWARE 1. Parts name and the function Arm Stand 4. Laser Unit 3. CMOS camera + Lens 2. Power supply 6. USB key 1. Computer-based Image Processor 1) Computer-based imager processor This computer-based imager processor shows black/white real images by laser light and tissue blood flow images calculated by the installed software.
Page 6 2) Power supply The power supply contains the laser drive circuit, the power supply for the CMOS camera and the intermediate connector for the CMOS camera. Turn on the laser switch after the laser unit and the CMOS camera were connected.
Page 7 3) CMOS camera and Lens The images are captured with the CMOS Mono-focal Lens camera. The hybrid filter is attached on the lens. This is the specialized filter combined of the polarizer and the color filter not to detect the CMOS camera direct reflection from tissue surface and the light from outside.
Page 8 4) Laser unit The laser diodes for measurement (infrared, 780 nm) and the positioning (red, 650 nm) are installed in the laser unit. The left side is the laser for measurement. The positioning laser can be turned off with the RL switch on the rear panel when it is not needed.
Page 9: Setup Of The Stand
2. Set up of the arm stand The arm stand is composed of the base, the cramp, the pole and the arm. (1) Install the cramp on the table used for measurement. (2) Stand the pole on the cramp Screws by the screws.
Page 10 The hold plate with the CMOS camera. (4) Install the hold plate with the CMOS camera on the arm stand by the four screws. (5) Attach the lens on the CMOS camera. Lens...
Page 11 (6) Install the laser unit on the hold plate by the screw knob. 専用ネジ (7) Connect the CMOS and the laser unit to the power supply by the 12 pin cables. For the GigE Ethernet cable (8)The CMOS camera has two inputs. One is for the 12-pin cable for the power supply, and the other is for the Ethernet cable for the signal.
Page 12 3. Wiring 1) Connection to the Computer-based image processor (1) Connect the AC power cable to the inlet on the left side. (2) Connect the Ethernet cable from the camera through the power supply to the Ethernet connector on the right side. (3) Connect the USB key to the USB connector on the left side.
Page 13 4. Adjustment of Lens The mono-focal lenses have the iris and the focus adjustment function. The zoom lenses have the function of zooming and the focus adjustment function. Mono-focal Zoom lens lens (1) Decide the measurement area, and set the WD (working distance). (2) Set the iris to “...
Page 14: Block Diagram
6. Block diagram 650 nm Laser 650 nm Laser diode drive circuit 780 nm Laser 780 nm Laser diode drive circuit...
Page 15: Lsi Software
LSI Software （LSI ver. 6.1） 1. Summary The LSI software is for measuring and saving blood flow images. This software consists of two views. ■Project Configuration File ■LSI Front Panel 2. Project Configuration File The display is shown after the LSI is opened. 1) Working Directory and File name The display shows the directory where images will be stored.
Page 16 2) File Name Open this “ Test “ folder and enter the file name for the image data being saved. Without assigning a specific name, “Image” will be the file name automatically. Do not use “BF” for the original file name because it is recognized as the “Blood Flow Image”...
Page 17 Actual fine name is created as” Image HR BF R3T~3500No001”. HR : Mode BF : Type of image R3 : Round T~3500 : Time No001 : Number o the image The specific file name that a user can assign is the place for “Image”. Do not change the name after the specific name.
Page 18 7) Used Disk Space Displays, in bytes, the used space of the disk containing the working directory. 8) Measurement Push the “ OK “ button to go to the “ LSI Front Pane l” or the “ Cancel ” button to exit the LSI program.
Page 19: Lsi Front Panel
3. LSI Front Pan LSI Front Panel Base display Example of measurement...
Page 20 1) Video Controls These are controls to display real and real-time images of blood flow images. Click “ Real Video “ switch to show the images. Click again to stop showing the images. In the Real Video mode, only real images are displayed at 60 frames per second.
Page 21 (3) HS-AVG : High Speed AVG This mode provides an average of “ X “ High Speed images, where “ X “ is an integer value that can be controlled by adjusting the “ Multi “ value in the HS AVG box. Resolution : 212 - 160 Name of the saved file : HSABF No The interval of saving images is set by “...
Page 22 4) File Management Controls There are functions for loading images and saving images. Pushing the “ Load Image “ button allows to showing the saved images. Select the image files from “ Blood Flow Images “ or “ Real Images “ folders in the folder saved images are stored.
Page 23 l Freehand Draws a freehand region. l ROI Clear Clears ROIs at once The tools can be set on both “ Real Image” display 8) and “ Blood Flow Image “ display 9). The ROIs show the average blood flow values in it. The averaged blood flow values are displayed in “Blood Flow Value “box 11), “...
Page 24 values of the axes. The maximum number of X-axis is 10,000. The slider bar at the bottom of the graph can be used to display a specific time (number) in the graph.. 13) Blood Flow Histogram Histogram of the number of pixels corresponds to blood blow values in ROIs. The X-axis shows blood flow value in ROI, and Y-axis shows the number of pixels at a blood flow value on the X-axis.
Page 25 (d) Load Images : Same function as “ Load Image “ button in 4). (e) Save Color BF Image : Color images are saved as tif files. Select the folder for saving the images. The color bar is not saved. (f) Exit : LSI is exited.
Page 26 4. Operation of LSI 1) Setup (1) Install the tissue to be measured under the laser unit and the CMOS camera with a lens. The relation between W. D., the distance between the tissue and the lens, and the measurement area is shown in the table in Appendix.
Page 27: Lia Software
LIA Software （LIA ver.6.0） 1. Summary This LIA software is for analyzing blood flow image data saved by the LSI software. This software consists of two views. ■ Analysis Configuration File ■ LIA Front Panel 3. Analysis Configuration File When the icon of LIA is double clicked, the first display appears. 1) Working Directory and File name Assign the folder for analyzing the blood flow data saved by LSI Pro.
Page 28 Choose the file, “ Image” file in this case, and click “ OK “. This “ Image “ file includes the information about the saved images in “ Test “ folder. The file name can be changed in LSI, but not in LIA. The next display, “...
Page 29 3) Free Disk Space This column shows the free volume remaining in the data disk containing the working directory. (When the total free disk space is below 30 Mega-bytes the user is warned) 4) Used Disk Space This column shows the used volume in the disk. 5) Previous Information The information described in the LSI.
Page 30: Lia Front Panel
4. LIA Front Panel LIA Front Panel Base display Example of analysis...
Page 31 1) File Management Controls There are functions for loading images and saving images. Pushing the “ Load Image “ button allows to show the saved images. Select the image files from “ Blood Flow Image “ or “ Real Image “ folders. The whole display can be saved by “...
Page 32 5) Tool Palette Tool Palette has the tools to enclose a region of interest (ROI). The LIA has 8 ROIs. The following tools can be selected. l Line Draws a line. l Rectangular Draws a rectangle or a square. l Oval Draws an oval or a circle.
Page 33 6) Blood Flow Value The average blood flow value determined from all blood flow value points within ROIs. Pushing the ZE(zero exception) button eliminates the zero flow from the calculation of the average blood flow. 7) Play Movie Plays the movie of an entire sequence images. The frame rate can be set. Push the “...
Page 34 the “ Display “ button. This is used to know the time when the treatment is done during the measurement. The markers are stamped as “ M “ in the Excel file made by “ Analyze Images” in “ Tools “ in the menu bar. 13) Function of the menu bar (1) File (a) Capture Screen : The whole display can be save in the clip board.
Page 35 Blue - Red : Used for differential mode 15). This color bar is useful to know the blood flow changes in a specific area. White : center color Blue : under the setting value Red : over the setting value. Over MAX : Colors of white or red can be selected for the blood flow value over the value set by “...
Page 36 (b) Loading ROI Select " Load " in " ROI" in the menu bar, and the list appears. Select the ROI in the folder. Click " OK ". The selected ROI appears on the display.
Page 37 Only one ROI can be attached on the image when the ROIs have the same number. ( example : only one ROI1 is shown o the image.) (c) Information of ROIs The information of saved ROIs can be obtained by selecting " Information ".
Page 38 The information is shown as the figure below. This information shows the ROIs appeared on the display. 14) Saving the setting and initial setting (1) “ Save Setting “ button saveと the setting of (a) Values of MAX and MIN, (b) Y axis of Blood Flow Plot, (c) Y axis of Histogram, (d) Figures and the positions of ROIs, and...
Page 39 15) Differential Image Function This function is for comparison of two blood flow images. The differential blood flow images are calculated from the control image. 1. Push “ Differential Mode “ button. 2. Push “ Load Image “ button. 3. Select the control image from saved “ BF images “ 4.
Page 40 5. Operaion of LIA 1) Start LIA software. Enter the information in the “ Analysis Configuration File “, and click “ OK “. 2) Push the “ Load Image “ button on the “ Front Panel “. 3) Select the folder to be analyzed and open it. 4) Select the blood flow image in the “...
Page 41: Handling In Use
Handling in use 1. Be careful to the next matters for install. (1) Install this instrument in the place where water does not hang. (2) Install this instrument in a place without fear to produce bad influence by atmospheric pressure, temperature, the humidity, ventilation, sunlight, dust, salt, and the air that includes sulfur.
Page 42 5. Do not remodel and modify. 6. Maintenance (1) Perform a periodic inspection by all means. (2) When the instrument was not used for a while, confirm that it operates normally and safely before use by all means.
Page 43: Appendix
Appendix 1. Table of measurement area by Lens for ver.6 Lens, WD and area for OZ-2 ver.6 Lens Zoom Area Lens Area Type (mm) (mm×mm) Type (mm) (mm×mm) Zoom ×10 6.2 x 4.8 Mono-focal 235 x 180 ZM10-18 ×1 60 x 44 290 x 220 ×10 10.4 x 8...
Page 44: Time For Saving Data And Capacity
2. Time for saving data and Capacity The relation between the size of the captured image and the capacity is described below. File size HDD capacity Capture Capture time [KB] [GB] Number 266,000 about about These are dependent on the condition of the HDD. 3.
Page 45: Specification
Specifications Environmental range for use and keeping 10 〜 35℃ Temperature range Humidity 30 〜 90% (non-condensing) Temperature for keeping -20 〜 60℃ Humidity for keeping 10 〜 90% (non-condensing) Atmospheric pressure 500 〜 1060 hPa Hardware Note type computer-based image processor Input voltage 115 (100～120V AC) / 230 (200～240VAC) ,50/60Hz 100W...
Page 46 Software LSI (Measurement) Mode Hi Res, Hi Speed, and HS-AVG 1) Hi Res about 2 image/sec display (High Resolution) 639 × 480 resolution 2) Hi Speed about 15 images/sec display (High Speed) 212 × 160 resolution 3) HS-AVG Averaging of Hi Speed mode (Hi Speed Average) 212 ×...
Page 47: Warranty
The warranty on this instrument, STD, shall be five (5) years from the day of purchase except the following conditions. 1) Wrong usage, the damage caused by an accident by carelessness 2) Damage and malfunction caused by fire, natural disaster and outside factors. OMEGAWAVE, INC. 2-20-3 Katamachi, Fuchu, Tokyo, Japan 183-0021...