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Summary of Contents for IOTA isoCell
- Page 1 For software versions 2.1.0 and 2.1.1 v2.1r4...
- Page 2 Products bearing the symbol of a strikethrough refuse bin must not be disposed of along with unsorted refuse. For details on how to dispose of your isoCell at the end of its useful life, see section 8.1. NOTE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules.
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Page 3: Table Of Contents
Starting-up ..........................................10 3.3.5 Restarting ........................................... 11 3.3.6 Powering-off .......................................... 11 Consumables ordering ......................................11 Using the isoCell as part of the iotaSciences Cloning Platform ................11 Workflow ................................................12 Isolate ................................................12 4.1.1 GRID ..............................................12 4.1.2 Plate ..............................................13 4.1.3... - Page 4 ® bottle ..................................23 STAR Replacing the dispensing assembly ..............................24 Troubleshooting ............................................24 Disposal and recycling ......................................... 25 Disposal of isoCell ........................................25 8.1.1 EU Waste Electrical and Electronic Equipment (WEEE) Directive ............. 25 8.1.2 Users outside the European Union ............................25 Disposal of FC40 ®...
- Page 5 10.3 Medical use restrictions ....................................... 27 10.4 Registered office ........................................... 27 10.5 Legal information ......................................... 27...
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Page 6: Introduction
Do not obstruct the movement of the isoCell’s needle holder, nor of the syringe plunger • mounts. Only use the isoCell when it is set on top of a safe, flat and stable horizontal surface. • If water or other liquid has been spilled near the isoCell, safety may be compromised. -
Page 7: Getting Started
If any foreign object enters the isoCell, safety may be compromised. Contact iotaSciences • for actions or precautions. Unplug the isoCell immediately if it smokes, or produces a strange odour or unusual noise. • Continued use may cause a fire. Contact iotaSciences for actions or precautions. - Page 8 Figure 1: Key components of the isoCell. (N.B. the selection pillar is by default undeployed and therefore may not be visible..)
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Page 9: Installation
6x tissue-culture treated 60mm dishes with attached locating rings • 12x eight-tube PCR strips • 25x 1.5ml microcentrifuge tubes • 1x pipetting aid • 1x 40ml bottle of FC40 ® • STAR Installation The isoCell should be located on a flat, stable surface inside a laminar flow hood. -
Page 10: Using The Isocell
2) is where the workflow of isolating, culturing and harvesting cells is executed. Figure 2: The isoCell bed, showing correct layout of 60mm dish, microcentrifuge tubes and 2x 8-tube PCR strips (optionally replaced with 2x flat bottom 8-well strips). The workflow is executed on a 60mm tissue culture dish (supplied in the CloneG consumables kit). -
Page 11: Running Programs
For instructions on the safe disposal of FC40STAR®, refer to Section 8.2. Cells are harvested by the isoCell from the 60mm dish into the two 8-tube PCR strips (or flat bottom 8-well strips) located at the front of the bed (Figure 2). -
Page 12: Restarting
At the end of the shutdown routine, the isoCell will indicate that power can be safely switched off. At this point the user should power-off the isoCell by pressing the power button (located on the top of the isoCell). -
Page 13: Workflow
Cloning Platform: Changes to the operation of the isoCell when using it as part of the iotaSciences Cloning Platform are indicated throughout this manual using an image of the isoHub. 4 Workflow The isoCell offers a convenient and user-friendly workflow to achieve consistent and reliable outcomes from single-cell cloning experiments. -
Page 14: Plate
Rather, best practice is for the user to agitate the sample until the isoCell prompts the user to place the tube onto the bed. This helps to prevent the cells from settling, thus maximising the adherence to Poisson statistics. -
Page 15: Culture
Once the GRID-coordinates of monoclonal chambers have been determined, the program is used to store these coordinates in the isoCell’s memory. The stages in the program are as follows: Select user . The user should select their own username. If the user is using the isoCell for •... -
Page 16: Harvest
At the beginning of the programs the user must select the monoclonal chambers from which to harvest colonies. detach or extract Cloning Platform: Prior to running the isoCell programs, use the isoHub prep detach prep extract programs to firstly inspect monoclonal chambers and... -
Page 17: Redetach
. See the for more details. 4.4 Chamber states As a dish progresses through the workflow, the isoCell tracks the state of the chambers. The current input data fill feed detach state of chambers in a dish is displayed at the beginning of the extract programs, and can be viewed at any time via the data program (Section 5.1). -
Page 18: Advanced Programs
The isoCell does not record the number (if any) of times the program has been run feed on a dish. The program will operate on chambers in a clonal filled state and will return them to a clonal filled state. -
Page 19: Replace Dispensing
( ) is used to prepare the isoCell for transit. It → should be used if the isoCell is to be packed and moved to a new location. isoCell transport bracket The user must ensure that they have an available;... -
Page 20: Reset Dispensing
) is used to calibrate the dispensing → → and jetting needle positions relative to the centre of the isoCell bed. It can be used if either of the isoCell’s needles has been replaced, to ensure continued performance of the isoCell. startup... -
Page 21: Options
move buttons will cause large/fast movements, whereas with it set to 0.01 the move buttons will cause small/slow movements. Figure 3: Needle holder control screen Figure 4: Syringe control screen 5.10 Options options more settings options program ( ) allows the user to: →... -
Page 22: Set Time
→ isoCell. Cloning Platform: When the isoCell is set up to sync data with an isoHub, it will get the time from the isoHub. To change the time on the isoCell, the time must be changed on the isoHub; the isoCell will then be updated with the new time when it next syncs data isoHub instruction manual with the isoHub. -
Page 23: Setting Up Data Syncing
The device name displayed at the bottom of both the data sync screen and the data sync status screen is the name of the isoCell that will be displayed on the isoHub. It can data sync... -
Page 24: About
Ensure the isoCell and isoHub are both switched on and both are displaying the main menu screen. data sync setup On the isoCell: run the program, click , and follow the onscreen instructions. If any problems arise, please contact iotaSciences for assistance. -
Page 25: Replacing The Dispensing Assembly
(Section 5.8) to ensure this. • Cloning Platform - if you are unable to find your user data on the isoCell, try pressing the • button in the top-right corner of the screen showing the list of usernames or dish colours. -
Page 26: Disposal And Recycling
8.1.2 Users outside the European Union To dispose of your isoCell at the end of its useful life, please refer to your local waste management organisation for proper disposal practices for used electronic and electrical equipment. 8.2 Disposal of FC40 ®... -
Page 27: Technical Support
If this product is found to be defective within that time, Iota Sciences Ltd may, in its sole discretion, at no additional or delivery cost, either repair or replace the unit, provide replacement parts, or issue a full refund and rescind the contract. This warranty does not cover accidental damage, wear and tear. - Page 28 (iii) will not infringe any third party’s intellectual property rights. 10.3 Medical use restrictions The isoCell is intended for research use by professionals only. The isoCell is not intended to be used for diagnostic purposes, or for testing or treatment in humans.
- Page 29 The isoCell is manufactured by Iota Sciences Ltd, Begbroke Science Park, Begbroke Hill, Woodstock Road, Yarnton, Oxfordshire, OX5 1PF, UK. isoHub®, isoCell®, FC40STAR®, and iotaSciences® are registered trademarks of Iota Sciences Ltd. The owners of any other marks mentioned in this manual (including any other marks associated with reagents) are not related to or affiliated with, and do not endorse or sponsor us.
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